OBJECTIVE Employing biological and chemical monitoring methods in steam sterilization qualification tests,to assure the qualification of the steam sterilizers. METHODS BI PCD and B-D test pack for three consecutive testings in empty chamber were used to qualify steam sterilizers. RESULTS Biological and chemical monitoring methods were the most effective monitoring techniques for steam sterility assurance. CONCLUSIONS The qualification tests should be executed exactly with the introduction of steam sterilizer in OR sterile supply division to assure medical safety.

OBJECTIVE To explore the process safety management of the details of its application,storage and disinfection.METHODS Poutine managements of every link of the EO disinfection Process such as the responsibility,operation procedure,regularly inspection,etc were carried out.RESULTS There were no accident occured since April,2004,when the EO disinfection machine began to work.Every monitoring sign was within the permitted scope.CONCLUSIONS Strictly management of every key procedure is the safety guarantee for the whole process of EO disinfection.

OBJECTIVE To explore the methods of management of ethylene oxide disinfection and evaluate its effects.METHODS The biological and chemical indicatons were used to monitor the effects in the disinfection process.The operating procedures were carried out strictly.RESULTS In 150 times disinfection processes 4.6% were not up to the standards in the two kinds of indicators.The main reason was incorrect operations in the whole process.CONCLUSIONS Strictly management and correct monitoring are very important to improve the disinfection quality for safety operation.

Genetic Heterogeneity ; Humans ; Magnetic Resonance Imaging ; methods ; Neoplasms ; diagnosis ; genetics ; pathology ; Positron-Emission Tomography ; methods ; Tomography, X-Ray Computed ; methods

Coronary Vessel Anomalies ; diagnosis ; Diagnostic Imaging ; Humans ; Myocardial Bridging

Objective:To study the impact of rotary cell culture system(RCCS) on the quality and activity of fetal islets of pancreas after cryopreservation and resuscitation.Methods:The fetal islets of pancreas were assigned into three groups averagely,experiment group 1,2 and control group.In experiment group1 and 2,fetal islets of pancreas were cultivated in RCCS and common culture respectively.While in control group,fresh fetal islets of pancreas were cultivated in RCCS all the time.We resected the fetal pancreas and digested with Collagenase V and purified with ficoll solution.Then standard cryopreservation step was adopted,and after resuscitation,the islets of pancreas were cultivated continuously.And the quantity,quality and activity of the islets of pancreas,and insulin stimulation test results in all groups were detected.Results:After purification,islets got from one fetal pancreas reached 2,331.98-5,115.43 IEQ(average 3,551.27? 253.76 IEQ) .The survival rate,the insulin release and stimulation index in islets of pancreas of group 1 incubated in RCCS were higher than those in common culture.Conclusion:RCCS are good for the growth and proliferation of islets of pancreas,and the islets of pancreas cultivated in RCCS have better insulin secreting capacity.RCCS combined standard cryopreservation method may further improve the cryopreservation effect.

OBJECTIVE:To establish a method for the content determination of 1-phenyl-7-(3-methoxy-4-hydroxy) phe-nyl-5-ol-3-heptanone in active ingredients of Mongolian medicine compound shudage-4. METHODS:HPLC was performed on the column of Intersil ODS-3 with the mobile phase of acetonitrile-0.5%phosphoric acid at the flow rate of 1.0 ml/min,the detection wavelength was 210 nm,temperature was 30 ℃,and the volume was 20 μl. RESULTS:The linear range of 1-phenyl-7-(3-me-thoxy-4-hydroxy) phenyl-5-ol-3-heptanone was 0.102-1.02 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.52%;average recovery was 97.10%(RSD=1.80%,n=6). CONCLUSIONS:The method is accurate,sim-ple and reproducible,and can provide basis for the quality control of Mongolian medicine compound shudage-4.

Objective To study the morphological changes of diaphragm of rats with omethoate poisoning and the protection of penehyclidine hydrochloride.Methods The experimental model of Wistar rats was made by eliac injection ofomethoate,96 rats was divided randomly into four groups in average:the hrinematched control group (Group NO);the omethoate intoxication matched control group (Group PO);atropine and pralidoxime chloride cure group (Group AC); penehyclidine hydrochlofide and pralidoximc chloride cure group (Group PC).The whole blood cholinesterase (ChE) and creatinekinase (CK) activtitise were measured 2 h after poisoning.To observe the morphological changes of diaphragmat different time from 1 to 7 days.Results All the poisoned rats showed that the diaphragmatic histologic damage of the penehyclidine hydrochloride and pralidoxime chloride cure group was slighter than that of atropine cure group.Conclusion A possible reasons of the respiratory muscle paralysis conduced by AOPP was the putrescence of diaphragm muscle fiber, and penehyclidine hydrochloride could definitely protect the diaphragm of rats with omethoate poisoning,and we could deduce that it prevented from intermediate myasthenia syndrome.

Objective To compare the biomechanical properties between the two-level fixation by im- plantation of pedicle-screws into the adjacent upper and lower vertebrae of the fractured vertebra and the three-level fixation by implantation of pedicle screws into the fractured vertebra and its adjacent upper and lower vertebrae in the treatment of thoracolumbar burst fractures.Methods Eight fresh frozen calf spines were used in this study.Each specimen was tested in four models:intact model as the control,L1 burst fracture model,two-level fixation model, and three-level fixation model.The L1 burst fracture model was created on a biaxial material testing machine (MTS858 Bionix test system,America).During the experiment,the flexion,extension,bilateral bending and axial rotation loadings were applied to the specimens and the range of motion(ROM)was measured with a three-dimensional laser analysis apparatus and the stiffness was calculated subsequently.One-way statistical analysis was used.Results The ROMs under six different loadings in the fracture model became larger obviously(P＜0.05)and the stiffness decreased(P＜0.05).The ROMs in both fixation models were smaller than those in the other models(P＜0.01)and the stiffness increased distinctly(P＜0.05).There were no significant differences in ROMs and stiffness between the two-level fixation and three-level fixation models(P＞0.05).Conclusion Two-level fixation provides similar biomechanical stability as three-level fixation does in the reconstruction of unstable thoracolumbar fractures.

Objective To study the expression of CDK4 andβ-Catenin and their relevance in glioma. Methods We used immunohistochemistry to detect the expression of CDK4 andβ-Catenin in forty-five glio-ma tissues and eight normal tissues.According to the classification standard of WHO in 2000 classify and grade the tissues.Results There were significant differences of CDK4 andβ-Catenin expressions between normal tis-sues and glioma tissues(P<0.01).The expression of CDK4 and β-Catenin had positive correlation with the pathological grades of glioma and histological type and increased(P <0.05).Furthermore,the expression of CDK4 was positively correlated with the expression ofβ-Catenin in glioma(r=0.52,P<0.01).Conclusion The increased expression of CDK4 andβ-Catenin may have correlation with malignant change of glioma and oc-curance of glioblastoma,and their combination is expected to become an important indicator in assessing malignant glioma.