1.Observation on clinical effects of acupuncture plus external medicine application for cervical radiculopathy
Ji-Xin WU ; Yong ZHI ; Ying LÜ
Journal of Acupuncture and Tuina Science 2018;16(3):171-175
Objective:To observe the clinical effects of acupuncture plus external medicine application for cervical radiculopathy.Methods:A total of 98 patients with cervical radiculopathy were randomly divided into an observation group and a control group based on the random digital table.The observation group (50 cases) was treated by acupuncture plus external medicine application and the control group (48 cases) was treated by acupuncture alone.The patients received the treatment every day.Ten sessions made a course.Cervical spondylosis symptom scale was used to assess the cases before and after the treatment.The clinical efficacy was compared between the two groups after the treatment.Results:The total effective rate was 98.1% in the observation group versus 87.6% in the control group with a statistically significant difference between the two groups (P<0.05).The cure rate was 52.0% in the observation group versus 18.8% in the control group with a significant difference between the two groups (P<0.05).There was no significant difference in the score of cervical spondylosis symptom scale between the two groups before the treatment (P>0.05).It increased with a significant difference in both groups after the treatment (both P<0.05),while a significant difference was found in the results between the two groups after the treatment (P<0.05).Conclusion:Acupuncture plus external medicine application is more effective than acupuncture alone for treating cervical radiculopathy.The method also effectively improves the clinical symptoms.
2.Comparison of surface marker of monocyte-derived dendritic cells between cord blood and adult peripheral blood
ji-hong, QIAN ; tong-xin, CHEN ; xi, WANG ; ying-ying, JIN ; jian-xing, ZHU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(10):-
0.05).The percentage of CD40 positive cells in CBMC-derived DC was lower than that in PBMC-derived DC[(34.80?7.77)% vs(54.37?9.57)%,P
3.Isolation and characterization of cancer stem cell-like sphere-forming cell subpopulation from the human nasopharyngeal cell line CNE-2
Tianxing JI ; Xin ZHANG ; Xiangyong LI ; Caihong LI ; Ying HUANG ; Keyuan ZHOU ; Tao LI
Chinese Journal of Clinical Oncology 2013;(13):754-757
Objective: The present study aimed to isolate and characterize a cancer stem cell-like sphere-forming cell subpopula-tion. Methods: By using a spheroid culture stem cell-conditioned medium, we isolated a subgroup of cancer stem-like cells from naso-pharyngeal cancer cell lines. Chemotherapy resistance was analyzed by using methyl thiazolyl tetrazolium, and clone-forming capabili-ty was determined by using softer agar clone formation assay. Finally, we verified the expression of the stemness-specific gene andβ-catenin by using immunocytochemistry staining and RT-PCR. Results: The lower-differentiated nasopharyngeal cancer lines con-tained more sphere-forming cells, whereas sphere-forming cells were not observed in the high differentiated nasopharyngeal cancer line CNE-1. Compared with CNE-2, CNE-2S (sphere-forming cells derived from CNE-2) exhibited higher chemotherapy resistance and clone-forming ability. Interestingly, the stemness genes BMI-1, ABCG2, and ALDH1 exhibited higher expression in CNE-2S than in CNE-2. β-catenin, a vital transcription factor of the WNT pathway related to stem cells, exhibited higher expression in CNE-2s cellular nucleus and plasma than in CNE-2. Conclusion: We isolated a subgroup of stem-like nasopharyngeal cancer sphere-forming cells. This discovery paves the way for the development of therapeutic strategies aimed at eradicating tumorigenic subpopulations in nasopharyn-geal cancer.
4.Multiple lymphomatous polyposis of intestine: report of a case.
Cai-qin WANG ; Zhong-xin SHI ; Jing JIANG ; Ji-hong ZHANG ; Ying ZHANG ; Qian WANG
Chinese Journal of Pathology 2011;40(5):341-342
Antigens, CD20
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metabolism
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CD5 Antigens
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metabolism
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Colonic Neoplasms
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complications
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metabolism
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pathology
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surgery
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Cyclin D1
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metabolism
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Diagnosis, Differential
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Female
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Humans
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Ileal Diseases
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complications
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pathology
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surgery
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Ileocecal Valve
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Intestinal Neoplasms
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complications
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metabolism
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pathology
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surgery
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Intestinal Polyps
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complications
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metabolism
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pathology
;
surgery
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Intussusception
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complications
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pathology
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surgery
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Leukemia, Lymphocytic, Chronic, B-Cell
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metabolism
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pathology
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Lymphoma, Mantle-Cell
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complications
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metabolism
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pathology
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surgery
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Middle Aged
5.Screening and taxonomic identification of endophytic fungi with antitumor and antioxidant activities from Artemisia lactiflora.
Yi-Xin QIAN ; Ji-Chuan KANG ; Bang-Xing LEI ; Lu WANG ; Ying HUANG
China Journal of Chinese Materia Medica 2014;39(3):438-441
Artemisia lactiflora is an important medicinal plant in China. The antitumor and antioxidant activities of the extracts of 54 endophytic fungi from the plant were screened via MTT assay and DPPH scavenging radical assay, respectively. The bioactive strains were identified based on similarity of 5.8S gene and internal transcribed spacer (ITS) sequences. The results showed that extracts from ten (18.5%) isolates exhibited antitumor activity, and which from two (3.7%) isolates exhibited antioxidant activity. The Alternaria sp. GYBH47 strain was simultaneously having antagonistic activity against HL-60 leukemia, MCF-7 breast and COLO205 colon cell lines, and Phomopsis sp. GYBH42 strain having cytotoxic and antioxidant activities. The results indicated that endophytic fungi from Artemisia lactiflora are potential resources to find valuable bioactive components.
Antineoplastic Agents
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chemistry
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pharmacology
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Artemisia
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microbiology
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Biphenyl Compounds
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metabolism
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Cell Line, Tumor
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Endophytes
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chemistry
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classification
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physiology
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Free Radical Scavengers
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chemistry
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pharmacology
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Fungi
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classification
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physiology
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Humans
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Picrates
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metabolism
6.Study on Preparation and Quality Standard of Jiekang Lotion
Ying JI ; Shailong NIU ; Zhilin WU ; Feng SHI ; Xin LIU ; Yilei GUO
China Pharmacy 2005;0(15):-
OBJECTIVE: To prepare gynecological Jiekang lotion and to establish its quality standard. METHODS: The Caffeotannic acid in Flos Lonicerae, the Berberine in Cortex Phellodendri and the Ammothamnine in Radix Sophorae Flavescentis were identified by TLC; HPLC was adopted to determine the content of Osthole with Hypersil DOS C18 as chromatograpic column. The mobile phase consisted of acetonitrile-water (54∶46) with a flow rate of 1.1mL? min-1. The detection wavelength was set at 321nm. RESULTS: Both the chief components in Jiekang lotion and the corresponding reference substances showed the identical discrimination reactions. The linear range of Osthole was 13~130?g?mL-1. CONCLUSION: The method is sensitive in qualitative reaction, specific in TLC identification, accurate in HPLC quantitation and recurrent. The established standard is applicable for the quality control of Jeikang lotion.
7.Evaluation and clinical significance of coronal bone structure matching in distal radius fractures
Xin ZHANG ; Jiaqing JI ; Ying YAO ; Shanzhu LI ; Jiaqian ZHOU ; Yong YIN ; Feng YUAN ; Jian FAN
Chinese Journal of Orthopaedics 2021;41(6):368-375
Objective:To study the coronal bone structure matching of distal radius in normal population and some patients with postoperative distal radius fracture, and to explore the clinical significance of coronal bone structure reduction of distal radius fracture.Methods:CT scans of 80 asymptomatic wrists were performed. Mimics 20.0 and 3-Matic research software were used to measure the matching data of coronal bone structure of the distal radius. Total of 44 patients with distal radius fractures treated with open reduction and volar plate fixation were collected. According to the data coronal bone structure of the distal radius, the matching group was in the normal range, and the mismatching group was less than the normal range. X-ray films were used to evaluate fracture healing, humeral height, ulnar angle and palm tilt angle at 3 months and 12 months after operation. The clinical indexes of wrist pain, wrist function, grip strength and activity were recorded in 2 groups. The DASH score was used for evaluation, and statistical comparisons was made between the two groups of related indicators.Results:The coronal bone structure matching value of the distal radius in 80 normal adults was 45.0%±16.2%. All the 44 patients with distal radius fracture were followed up for an average of 16 months. The postoperative wound healing was good, and the bone healing standard was reached 3 months after the operation. 3 months after surgery, radius height, ulnar deviation angle and palmar inclination angle of the mismatched group were all smaller than those of the matched group, but the differences had no statistical significance. The pronation angle in the mismatched group (68.82°±11.62°) was lower than that in the matched group (76.91°±9.14°), and the difference was statistically significant ( t=2.567, P=0.014). The DASH score in the mismatched group (15.53±2.36) was higher than that in the matched group (13.62±2.52), and the difference was statistically significant ( t=2.591, P=0.013). 12 months after surgery, the VAS score of the matched group (2.08±2.95) was less than that of the mismatched group (2.95±1.24), and the difference was statistically significant ( t=2.348, P=0.024). There was no significant difference in wrist range of motion, grip strength and DASH score between the two groups. Conclusion:The coronal bone structure matching of distal radius is about 45.0% in normal population. Early wrist dysfunction, limited pronation, and wrist pain may occur when the postoperative matching degree of the distal radius fracture is not within the normal range.
8.Protective effect of chrysin regulates AMPK-NLRP3 signaling mediated pyroptosis to alleviate hepatic fibrosis
Yu-xin ZHANG ; Hao-lin GUO ; Ji-feng LI ; Ying DONG ; Yong YANG ; Ting BAI
Acta Pharmaceutica Sinica 2023;57(9):2669-2676
This study investigated the protective effect of chrysin on hepatic fibrosis by regulating AMP-activated kinase (AMPK)-NOD-like receptor protein 3 (NLRP3) mediated pyroptosis pathway. The hepatic fibrosis model of mice was established by thioacetamide (TAA)
9.Serum-free media for articular chondrocytes in vitro expansion.
Xin-xin SHAO ; Neil A DUNCAN ; Lin LIN ; Xin FU ; Ji-ying ZHANG ; Chang-long YU
Chinese Medical Journal 2013;126(13):2523-2529
BACKGROUNDIn vitro chondrocyte expansion is a major challenge in cell-based therapy for human articular cartilage repair. Classical culture conditions usually use animal serum as a medium supplement, which raises a number of undesirable questions. In the present study, two kinds of defined, serum-free media were developed to expand chondrocytes in monolayer culture for the purpose of cartilage tissue engineering.
METHODSBovine chondrocytes were expanded in serum-free media supplemented with fibroblast growth factor-2 and platelet-derived growth factor or fibroblast growth factor-2 and insulin-like growth factor. Expansion culture in a conventional 10% fetal bovine serum (FBS) medium served as control. Fibronectin coating was used to help cell adhesion in serum-free medium. Next, in vitro three-dimensional pellet culture was used to evaluate the chondrocyte capacity. Cell pellets were expanded in different media to re-express the differentiated phenotype (re-differentiation) and to form cartilaginous tissue. The pellets were assessed by glycosaminoglycans contents, collagen II, collagen I and collagen X immunohistological staining.
RESULTSChondrocytes cultured in serum-free media showed no proliferation difference than cells grown with 10% FBS medium. In addition, chondrocytes expanded in both serum-free media expressed more differentiated phenotypes at the end of monolayer culture, as indicated by higher gene expression ratios of collagen type II to collagen type I. Pellets derived from chondrocytes cultured in both serum-free media displayed comparable chondrogenic capacities to pellets from cells expanded in 10% FBS medium.
CONCLUSIONThese findings provide alternative culture approaches for chondrocytes in vitro expansion, which may benefit the clinical use of autologous chondrocytes implantation.
Animals ; Cartilage, Articular ; cytology ; Cattle ; Cell Dedifferentiation ; Cells, Cultured ; Chondrocytes ; cytology ; physiology ; Culture Media, Serum-Free ; Fibronectins ; pharmacology ; Real-Time Polymerase Chain Reaction ; SOX9 Transcription Factor ; genetics
10.Progress of sulfur fumigation and modern processing technology of Chinese traditional medicines.
Tu-Lin LU ; Xin SHAN ; Lin LI ; Chun-Qin MAO ; De JI ; Fang-Zhou YIN ; Yong-Ying LANG
China Journal of Chinese Materia Medica 2014;39(15):2791-2795
Infestation, moldy and other phenomenon in the processing and storage of Chinese herbal medicines is a problem that faced in the production of Chinese traditional medicine. The low productivity of traditional processing methods can not guarantee the quality of Chinese herbal medicines. Sulfur fumigation is the first choice of grassroots to process the Chinese herbal medicine with its low cost and easy operation. Sulfur fumigation can solve some problems in the processing and storage of Chinese herbal medicines, but modern pharmacological studies show that long-term use of Chinese traditional medicine which is fumigated by sulfur can cause some serious harm to human liver, kidney and other organs. This paper conducts a review about the application history of sulfur fumigation, its influence to the quality of Chinese herbal medicines as well as domestic and foreign limits to sulfur quantity, and a brief introduction of the status of modern processing technologies in the processing of food and some Chinese herbal medicines, the problems ex- isting in the Chinese herbal medicines processing, which can provide a reference basis for the further research, development and application of investigating alternative technologies of sulfur fumigation.
Fumigation
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adverse effects
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methods
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Medicine, Chinese Traditional
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methods
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Quality Control
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Social Control, Formal
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Sulfur
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chemistry
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Technology, Pharmaceutical
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methods