Objective: To study the anti-tumor constituents from the roots of Actinidia valvata Dunn. Methods: Column chromatographic techniques were used to isolate and purify the chemical constituents of the plant and their structures were elucidated by spectroscopic analysis, including 1HNMR, 13CNMR and MS. The cytotoxic activities of some individual compounds were examined by MTT assay. Results: Ten triterpenoids: 2α, 3α, 24-trihydroxyurs-12-en-28-oic acid(1), asiatic acid(2), corosolic acid(3), 2α, 3α, 23, 24-tetrahydroxyurs-12-en-28-oic acid(4), 2α, 3α, 13β, 24-tetrahydroxyurs-11-en-28-oic acid-13-lactone(5), 2α, 3α, 24-trihydroxyolean-12-en-28-oic acid(6), 2α, 3α, 19α, 24-tetrahydroxyurs-12-en-28-oic acid(7), 2α, 3α, 24-trihydroxyurs-12, 20(30)-dien-28-oic acid(8), 2α, 3β, 24-trihydroxyurs-12-en-28-oic acid(9), and oleanolic acid (10), and one plant sterol β-citosterol(11) were isolated from the CHCl3 fraction of the roots of Actinidia valvata Dunn. The cytotoxic activities of five compounds (1, 2, 3, 4, and 7) against A549, LOVO and HepG2 cell lines were evaluated. Conclusion: Compounds 3-8 are isolated from this plant for the first time. Compounds 2 and 3 possess cytotoxic activity against LOVO and HepG2 cell lines, and the cytotoxic activity decreases with the increase of polarity of individual compound.

OBJECTIVE: To provide references for the correct understanding and accurate implementation of the physical and chemical analysis general chapters of the Chinese Pharmacopoeia 2020 Edition volume . METHODSE: The principles, processes, and main considerations of the development of the physical and chemical analysis general chapters of the Chinese Pharmacopoeia 2020 edition volume IV are introduced, and the content and main characteristics are summarized and analyzed. RESULTS: According to the goals and key tasks of the Chinese Pharmacopoeia 2020 edition, the principles of scientificity, versatility, operability, gradual and sustainable development, and harmonization with international standards, the physical and chemical analysis committee of the Chinese Pharmacopoeia commission carry out the development of the physical and chemical analysis general chapters of the Chinese Pharmacopoeia 2020 edition volume . Further expand the application of advanced and mature analytical techniques, strengthen the applicability of analytical methods and the development of the external pollution control methods for Chinese medicinal materials, and improve the harmonization of analytical techniques and impurity control requirements with international technical requirements. CONCLUSION: The physical and chemical analysis general chapters of Chinese Pharmacopoeia 2020 edition volume provide method and technical support to ensure the safety, effectiveness and controllable quality of China's pharmaceuticals, and play an important and positive role in encouraging the application of advanced analytical technology, strengthening drug regulatory measures, and enhancing the international influence of the Chinese Pharmacopoeia.

OBJECTIVE: To provide reference for introducing new methods and technologies to the physical and chemical testing methods general chapters in Chinese Pharmacopoeia Volume . METHODS: By reviewing the general chapters of drug physical and chemical testing methods (appendices)in the new editions of European, US, Japanese and British Pharmacopoeias, the harmonization results of the Pharmacopoeia Discussion Group (PDG)and the guidelines of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH), the authors summarized the overview and new, revised and harmonized texts of physical and chemical testing methods, and made a preliminary comparison with the related testing methods in Chinese Pharmacopoeia (2015 edition) Volume . RESULTS: The new and revised text on the physical and chemical testing methods in the new editions of European, US, Japanese and British Pharmacopoeias are mainly concentrated in elements impurities, spectrometry and chemometrics. Chinese Pharmacopoeia can learn from the technology of identification tests, spectrometry and chromatography, impurities analysis and functionality-related characteristics of pharmaceutical excipients. CONCLUSION: Chinese Pharmacopoeia should be based on the actual level of China's pharmaceutical industry, follow the principles of progressive and sustainable development, introducing new drug testing technologies and methods to strengthen the quality control of drugs and improve drug controllability.

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This article studies the status quo of professional psychological pressures among 211 doctors in Peking University and influencing factors.It can be inferred that the most important source for professional psychological pressures comes from overloaded job.Certain countermeasures are also raised from a humanistic perspective on national,social,organizational,and individual levels,aiming to relieve clinicians' professional psychological pressures and fully develop their working potentials.

Objective In this study,the authers investigate the effect of lumbar arachnoid space continuous drainage to prevent and reverse cerebral vasospasm (CVS) angiographically following experimental subarachnoid hemorrhage (SAH).Methods SAH model was achieved by cisternal magna twice injections of autolugous blood.Thirty adult mongrel dogs presented with SAH were randomly divided into five groups: early-drainage group,later-drainage group,early-puncture group,later-puncture group and control group. The lumbar puncture two groups started to puncture and exclude blood cerebral spinal fluid(CSF) 2 hours later following the second injection and on day 5 respectively,while the drainage two groups started to drain at the same time.The degrees of CVS were analyzed through cerebral angiography(%reduction of basilar artery diamiter,%RBAD). Results ①Compared with early puncture group and control group,early drainage group had less cases of CVS and the degrees of CVS were more slight which relieved within shorter period than other two groups.②Compared with later puncture group and control group,later drainage group had less cases of CVS and the period of CVS was shorter.③Compared with later drainage group,there were less cases of CVS and the degrees of CVS were more slight in early drainage group.Conclusions Lumbar subarachnoid space continuous drainage may prevent and reverse the CVS following SAH.Early lumbar subarachnoid space continuous drainage should be presented with better consequence.

Objective To observe the impact of Aspergillus fumigatus conidia on the early apoptosis of alveolar epithelial cells.Methods A549 cells was first stimulated with tumor necrosis factor-α(TNF-α)or staurosporine (STS)to induce cell apoptosis,and then co-cultured with Aspergillus fumigatus conidia of three different concentrations (5 × 10 5 ,5 × 10 6 ,5 × 10 7 )simultaneously.The changes of the apoptosis rate of A549 cells were detected by flow cytometry.Results The early apoptosis of A549 cells was increased markedly after stimulated with TNF-α ([14.94 ± 0.82 ]% vs [3.26 ± 0.74]%,χ2 =9.18,P <0.05)and STS ([18.10±1 .00]% vs [3.26±0.74]%,χ2 =12.32,P <0.05). When Aspergillus fumigatus conidia of different concentrations were added,the apoptosis rate was decreased (χ2 =31 .23,P <0.05 ).The early apoptosis of A549 cells was negatively correlated with the concentration of Aspergillus fumigatus conidia (rTNF = - 0.92,r STS = - 0.94,both P < 0.05 ). Conclusion Aspergillus fumigatus conidia inhibits the early apoptosis of A549 cells induced by TNF-αor STS and the inhibit effect increases with the concentration of Aspergillus fumigatus conidia.

Objective To discuss the feasibility and safety of treatment on acute ruptured wide necked intracranial aneurysms with endovascular stent and Guglielmi detachable coils (GDS).Methods The coronary stents were first implanted across the neck of 57 acute ruptured wide necked intracranial aneurysms with microcatheters introducing into the aneurysm sac through the stent mesh. GDCs were used to embolize the aneurysms. Results 56 procedures were successful with only one failure of stent implantation due to tortuous vessel. Over 90% occlusion were achived in 56 embolized aneurysms. The patients recovered well with patency of the parent arteries.Conclusion Endovascular therapy with stent implatation and GDC placement is a safe and feasible approach for the treatment of acute ruptured wide-necked intracranial aneurysms.

Objective To observe the growth inhibiting effect of tea polyphenols on implanted mouse breast cancer(EMT_6),and offer the foundation for the next study on its inhibiting angiogenesis effect and its molecular mechanism.Methods Mammary adenocarcinoma EMT_6 was cultured and propagated,then implanted on female BALB/c mice.Tea polyphenols was peroral administrated and local injected.Anticancer function was determined with regular methods.Result After tea polyphenols were taken by oral and local injection on EMT_6 mammary adenocarcinoma mice,the inhibitory ratio of tumor are respectively 37.43% and 40.94%.Conclusion Tea polyphenols had significant inhibitive effect on implanted breast cancer.This offered the foundation for the next intensiVe study on its inhibiting angiogenesis effect and its molecular mechanism.

Objective:To optimize the determination technology for the total amino acids in Runing pills. Methods:The process was optimized by L9 (34 ) orthogonal design using the hydrolysis temperature, hydrolysis time and hydrochloric acid concentration as the evaluation factors,and the content and yield of total amino acids as the evaluation indices. Results: The optimal determination condi-tions were as follows:the hydrolysis temperature was 110℃, the hydrolysis time was 22 h and the hydrochloric acid concentration was 6 mol·L-1 . Conclusion:The optimal determination technology can be applied in the total amino acids from Runing pills,which pro-vides the basis for the further research of Runing pills.