3.Combined Effect of Arsenite and Cigarette Smoke Solution on NF-?B in Lymphocytes of Rats
Journal of Environment and Health 2007;0(09):-
Objective To investigate the effect of sodium arsenite combined with cigarette smoke solution on NF-?B in rat lymphocytes. Methods Rat lymphocytes were divided into 4 groups: the arsenite treatment group, the CSS treatment group, the arsenite and CSS treatment group, and the control group. Electrophoretic mobility shift assays was used to detect levels of NF-?B DNA binding. Western blot was used to detect protein expression of I?B?. Results Levels of NF-?B DNA binding in the CSS treatment group and the arsenite treatment group were significantly increased (P
4.Combined Use of Allergen Skin Prick Test and Specific Immunoglobulin E Antibody on Allergen Diagnosis in Asthmatic Children
li, XIANG ; zhen, LI ; yi-xin, REN
Journal of Applied Clinical Pediatrics 2006;0(21):-
Objective To analyze the advantages of combined analysis of allergen skin prick test(SPT)and phadiatop/specific IgE antibody on the allergen diagnosis in asthmatic children.Methods Inhalant allergen SPT and Phadiatop test were done in 57 asthmatic children.Thirty-three cases of those asthmatic children were measured serum specific IgE antibody against dermatophagoids pteronyssinus.Results Dermatophagoids,molds and pets were the main inhalant allengens in asthmatic children.The positive rates of SPT and Pha-(diatop) in 57 asthmatic children were 86% and 79%,respectively,and the consistence rate between SPT and Phadiatop was 86%.Five cases with negative Phadiatop were confirmed to have molds allergy via SPT and molds specific IgE test.The consistence rate of dermatophagoids pteronyssinus SPT and specific IgE was 97%.Conclusion It is helped to improve the sensitivity and specificity of allergen diagnosis in asthmatic children when doctors combined analyze the results of allergen skin prick test and specific IgE test.
6.Diagnostic values of antibody to citrullinated human fibrinogen in rheumatoid arthritis
Yi ZHAO ; Xin TIAN ; Zhanguo LI
Journal of Peking University(Health Sciences) 2003;0(04):-
Objective: To study the prevalence of antibody to citrullinated human fibrinogen(ACF) in rheumatic diseases and the diagnostic values in rheumatoid arthritis.Methods: Human fibrinogen was deiminated by the peptidylarginine deiminase(PAD) in the presence of Ca~(2+) in vitro.ACF was detected by ELISA in 352 patients with rheumatic diseases(183 RA,121 SLE and 48 OA) and 108 healthy controls.The sensitivity and specificity in RA were calculated and the correlations between ACF and the clinical and laboratory parameters were analysed by SPSS statistical software.Results: Compared with SLE(25.62%),OA(18.75%) and healthy control(2.78%) respectively,the patients with RA had a significantly higher level of ACF(67.21%,P
7.Cytogenetic Toxic Effects of Copper Sulfate on Root Tip Cells of Zea mays L
Lan YI ; Guoqing LI ; Xin ZHAO
Journal of Environment and Health 1993;0(03):-
Objective To investigate the cytogenetic toxic effects of copper sulfate on root tip cells of Zea mays L.Methods The root tip cells of Zea mays L were treated by copper sulfate as mutagen at the doses of 0.05,0.10,0.25,0.50,1.00 g /L for 24 hours.The mitotic index,the micronucleus rate and the chromosome aberration rate of tip cells of Zea mays L were measured.Results Compared with the negative control group,the micronucleus rates and the mitotic index in 0.05,0.10,0.25,0.50,1.00 g /L groups and the chromosome aberration rates in 0.05,0.10,0.25,0.50 g /L groups increased significantly(P
9.Effect of cardiac troponin T on prognosis in the elderly patients with chronic heart failure
Xin YI ; Feng-Huan HU ; Li-Xin ZHANG ; Lei AN ;
Chinese Journal of General Practitioners 2003;0(05):-
Objective To study the effect of cardiac troponin T(cTnT)on the prognosis of chronic heart failure in the elderly patients.Methods Serum level of cTnT,and serum activities of creatine kinase (CK)and creatine kinase-MB(CK-MB)were measured in 60 elderly patients of chronic heart failure with varied classes of cardiac function.Cardiac events,including malignant cardiac arrhythemia,repeated or aggravated heart failure or cardiac death,in patients with increased cTnT during hospitalization were compared to those without it.Results Serum level averaged(89.1?13.6)mmol/L for CK and(7.9? 1.4)mmol/L for CK-MB in 20 patients with Class Ⅱ cardiac function by New York Heart Association (NYHA)classification and without increased cTnT,(88.2?13.4)mmol/L for CK and(7.5?1.8) mmol/L for CK-MB in 19 patients with Class Ⅲ cardiac function and four of them with increased cTnT,and (93.3?14.2)mmol/L for CK and(8.1?1.6)mmol/L for CK-MB in 21 patients with Class Ⅳ cardiac function and 14 of them with increased cTnT.Malignant cardiac arrhythemia occurred in six,repeated heart failure in eight,and death in three of 18 patients with increased cTnT,and one,two and one in 22 patients without increased cTnT,respectively.Incidence of cardiac events was higher in patients with increased cTnT than those without it(P
10.Percutaneous transluminal septal myocardial ablation versus septal myectomy for treatment of patients with hypertrophic obstructive cardiomyopathy: a Meta-analysis
Xin YI ; Xuejun JIANG ; Xiaoyan LI ; Yi GUO ; Gaoke FENG
Chinese Journal of General Practitioners 2013;(6):455-460
Objective To evaluate the efficacy of percutaneous transluminal septal myocardial ablation (PTSMA) and septal myectomy (SM) for treatment of hypertrophic obstructive cardiomyopathy.Methods We searched all clinical controlled trials of PTSMA and SM in treatment of hypertrophic obstructive cardiomyopathy in Cochrane Library,PubMed,Web of Science,Wanfang Database and CNKI update to August,2012.The selected studies in Chinese and English were used for Meta-analysis with software RevMan 5.0.Results Five studies including 286 cases (PTSMA:149,SM:137) were included in the study.Left ventricular outflow tract pressure was significantly reduced by SM treatment compared with PTSMA (OR =9.07,95% CI:0.96-17.18,P =0.030).There were no significant differences in interventricular septum thickness (OR =1.15,95% CI:-0.79-3.10,P =0.240),left atrial diameter (OR =1.95,95% CI:-0.16-4.07,P =0.070),left ventricular end-diastolic (OR =0.70,95% CI:-0.79-2.19,P =0.360),end systolic diameter (OR =1.14,95 % CI:-1.65-3.93,P =0.420),degree of symptom improvement (chest pain:OR =1.07,95 % CI:0.35-3.23,P =0.910;syncope:OR =0.32,95% CI:0.08-1.23,P =0.100),and postoperative mortality (OR =1.63,95%CI:0.46-5.76,P =0.450) between PTSMA and SM treatment; but the rates of pacemaker implantation (OR =5.91,95% CI:2.04-17.11,P =0.001) and reintervention treatment (OR =6.62,95%CI:1.20-36.62,P=0.030) in patients with PTMSA were significantly higher than those with SM.Conclusions There is no evidence to indicate that PTSMA can completely replace SM,as an alternative choice the therapy can be used according to left ventricular out flow tract pressure,symptoms,anatomy of target vessel and other conditions.