OBJECTIVE:To evaluate the efficacy and safety of hydrochloric fasudil(administered at different time after onset)for acute cerebral infarction(ACI).METHODS:90 patients developed ACI within 72 h were randomly divided into trial group and control group of 45 each.The trial group was subdivided into trial group 1(developed ACI within 24 h)and trial group 2(developed ACI for more than 24 h).The control group received Compound Radix Salviae Miltiorrhizae injection for 15 days while the trial groups received Fasudil Hydrochloride for 15 days.Improvement of neurological function was evaluated using China Stroke Scales(CSS).RESULTS:The CSS of trial group 1 and trial group 2 was significantly decreased as compared with that of control group,and the CSS in trial group 1 was markedly decreased than in trial group 2,all showing significant differences(P

Objective To explore the influence of lifestyle intervention on obesity and diet behavior in children with simple obesity.Methods 80 simple obesity children were chosen as research subjects,the parents were given cognitive behavior intervention,the children were given lifestyle interventions to control weight.The changes of obesity degree and dietary behavior were observed after the interventions.Results After the intervention,the parents in the adjustment of dietary structure,limit the time of watching TV,limit of snack in.take were significantly higher than those before intervention(P ＜ 0.05).Children reduced meat,snack intake,watch TV time more than those before intervention(P ＜ 0.05).Obesity degree was significantly lower than that before intervention(P ＜ 0.05).Conclusion The lifestyle intervention is beneficial to change unhealthy life style in obese children,and to reduce weight.

Objective: To analyze the regional differences of the health resource allocation in Guangdong Province from 2002 to 2011, and provide the scientific basis for regional health planning and health resource allocation optimizing. Methods: The Theil Index was used to analyze the regional differences of the health resource allocation in Guangdong Province. Results: The health resource per capital is consistent with the national level, while differences existed in different areas, and the gap of the differences has been broaden during this period. Conclusions: The differences are caused by the difference distribution of material resources, human resources, the faulty of system and mechanism. Therefore, the hygienic resources disposition should be balanced establish reasonable mechanism to improve the equity of health human resources, strengthen the community health service as a breakthrough during the health system reform, and promote the equal access to basic medical and health services to narrow the gap of the regional differences and promote the equity of health resource allocation.

In this study, we aim to investigate the protective effects of astragaloside on ultraviolet A (UVA)-induced photoaging in human fibroblasts and its possible mechanisms.

Objective To observe the changes of eosinophils (EOS) in peripheral blood in patients with acute myocardial infarction (AMI).To investingate the association of EOS in peripheral blood and acute myocardial infarction.Methods The single-centre study enrolled 50 acute myocardial infarction patients (23 cases were NSTEMI and 27 cases were STEMI) who were treated in Beijing Tiantan Hospital from January 2014 to July 2014.Fifty medical individuals who were randomly chosen from the same period of time.White blood cell counts,EOS counts and percentages, levels of Troponin Ⅰ of all individuals were determined.Compare White blood cell counts,EOS counts and percentages between two groups.Association of EOS in peripheral blood and Troponin Ⅰ in patients with acute myocardial infarction was evaluated by Pearson correlation.Results The levels of eosinophils counts ((0.02±0.01) × 109/L vs.(0.24±0.14) × 109/L) and percentages ((0.40±0.49) ％ vs.(1.25±0.54)％) in AMI patients were significantly lower than those in the control group (t =11.083, P＜0.01;t=8.242,P＜0.01).There was significant inverse correlation between EOS counts and percentage and levels of Troponin Ⅰ(r=-0.390,-0.387;P＜0.05).Conclusion Patients with acute myocardial infarction presented decreased eosinophils counts and percentages.EOS counts and percentage and levels of Troponin Ⅰ presented negative correlation.It was close relation of EOS and acute myocardial infarction.

OBJECTIVE:To explore the effects of Compound Ligusticum chuanxiong drop pill on hemorheology and blood gas in model rats with diabetic nephropathy(DN). METHODS:60 rats were randomly divided into normal control group,model group, positive control group [Irbesartan tablet,0.013 g/(kg·d)] and Compound L. chuanxiong drop pill high-dose,medium-dose,low-dose groups [0.4,0.2,0.1 g/(kg·d)],10 in each group. Except for normal control group,rats in other groups were given high-sugar high-fat feed and intraperitoneally injected streptozotocin to reduce DN model. After 7 d modeling,rats in each group received relat-ed drugs intragastrically,once a day,10 mL/kg;normal control group and model group received equal volume of purified water in-tragastrically. After 64 d,hemorheology indicators [whole blood viscosity(high-shear,medium-shear and low-shear),plasma viscosi-ty,whole blood reduced viscosity] and blood gas indicators(carbon dioxide partial pressure,oxygen partial pressure,hemoglobin content,oxygen saturation,ratio of oxygenated hemoglobin to total protein) of rats were determined,hematoxylin-eosin staining was used to observe the pathological changes in kidney tissue. RESULTS:Compared with normal control group,hemorheology indi-cators and carbon dioxide partial pressure in model group were significantly increased(P<0.05 or P<0.01),oxygen partial pres-sure,hemoglobin content,oxygen saturation,ratio of oxygenated hemoglobin to total protein were significantly decreased(P<0.05 or P<0.01),with severe kidney damage. Compared with model group,except the improvement of carbon dioxide partial pressure, oxygen partial pressure,hemoglobin content in rats were not significant in Compound L. chuanxiong drop pill low-dose group,the above-mentioned indicators in other medicine groups were significantly improved(P<0.05 or P<0.01);the kidney tissue damage was improved to varying degree. CONCLUSIONS:Compound L. chuanxiong drop pill can significantly improve the hemorheology indicators of DN model rats,reduce blood viscosity,improve microcirculation and capacity of the blood to transport oxygen,and has certain improvement effect on kidney tissue morphology of DN rats.

Objective To investigate the expressions of excision repair cross complementation group 1 ( ERCC1) and Ki67 in patients with breast cancer, and the relationships between their expressions and sensitivity of platinum-based chemotherapy. Methods Totally, 129 cases were pathologically diagnosed as breast cancer.Paclitaxel and carboplatin were used simultaneously. Chemotherapy regimen was as follows:Gemcitabine 1 000 mg??( m2 )-1 , IV drop on day 1 and 8;cisplatin 25 mg??( m2 )-1 , IV drop on day 1-3, for six cycles ( 21 days a cycle ) . ERCC1 and Ki67 expression in tumor tissue was observed by immunohistochemical analysis.Platinum-based chemotherapy sensitivity and survival of patients with different levels of ERCC1 and Ki67 expression were analyzed. Results In 129 patients, 18 cases were ERCC1 and Ki67 double-negative ( ERCC1-Ki67-) , and the clinical effective rate and 3-year cumulative survival rate were 88.89%and 83.33%, respectively.Twenty-four cases were ERCC1 positive but Ki67 negative ( ERCC1+Ki67-) , and the clinical effective rate and 3-year cumulative survival rate were 50. 00% and 62.50%, respectively.Thirty-three cases were ERCC1 negative but Ki67 positive (ERCC1-Ki67+), and the clinical effective rate and 3-year cumulative survival rate were 54. 55% and 60. 60%, respectively. Fifty-four patients were ERCC1 and Ki67 double-positive ( ERCC1+Ki67+) , and the clinical effective rate and 3-year cumulative survival rate were 22.78% and 31. 48%, respectively.Compared with ERCC1-Ki67- group, the clinical treatment efficiencies of cisplatin-based chemotherapy in ERCC1+Ki67- group, ERCC1-Ki67+ group, and ERCC1+Ki67+ group were significantly decreased ( P<0. 05 ) . The clinical treatment efficiency in patients of ERCC1+Ki67+ group with cisplatin-based chemotherapy was significantly decreased as compared with ERCC1+Ki67- group and ERCC1-Ki67+ group (P<0.05).Compared with ERCC1- Ki67- group, three-year cumulative survival rate in patients of ERCC1+ Ki67- group and ERCC1- Ki67+ group, ERCC1+Ki67+ group was significantly decreased ( P<0. 05 ) . Compared with ERCC1+Ki67-group and ERCC1-Ki67+group, three-year cumulative survival rate in patients of the ERCC1+Ki67+group was significantly decreased ( P<0.05) . Conclusion The expression levels of ERCC1 and Ki67 in breast cancer were high. Their expression levels are closely related with clinical efficiency of platinum-based chemotherapy.

Objective To discuss the relationships of the genotype of cytochrome P450 2C19 (CYP2C19) gene and and clopidogrel responsiveness.Methods The study enrolled 110 patients with acute myocardial infarction who were treated with clopidogrel in Tiantan Hospital of Beijing from November 2012 to May 2014.Patients were treated by aspirin and clopidogrel.CYP2C19 polymorphisms were detected by genotype testing kits.Platelet inhibition rates were measured by thrombelastography to represent the antiplatelet effect of clopidogrel.The platelet inhibition rates of clopidogrel were compared among different genotypes.Results Compared with carriers of CYP2C19 * 2 or * 3 reduced-function allele,CYP2C19 * 1 wild type had higher platelet inhibition rate of clopidogrel ((35.73 ± 19.29)％ vs.(48.30± 20.84)％),and the difference was significant(t =3.264,P＜0.05).There was no difference between intermediate metabolizer((35.72± 19.27)％) and poor metabolizer((35.75±19.89)％;P＞0.05).The frequency of wild-type homozygotes CYP2C19 * 1/* 1 was higher in responders than in low responders(frequency of low reaction group CYP2C19 * 1/* 1 14 cases (40.0％),* 1/.2 10 cases(28.6％),* 1/* 3 4 cases(11.4％),* 2/* 2 5 cases(14.3％),* 2/* 3 2 cases (5.7％),frequency of reaction group were 45 cases (60.0％),15 cases (20.0％),4 cases (5.3 ％),7 cases (9.3％),4 cases(5.3％);x2 =3.838,P =0.05).Conclusion Polymorphism of gene CYP2C19 is associated with different responses to clopidogrel.CYP2C19 * 2/ * 3 reduced-function allele is associated with low response to clopidogrel.

The present study describes systematically the ultrastructural changes of blood and some organs of rabbits, goats and rats with severe decompression sickness. In our experiment, the animals were exposed to pressure of 0.6 MPa and 1.0 MPa for 60 minutes respectively, to induce severe decompression sickness. The results are as follows: 1. Abnormality of platelets. The granules of platelets decreased and the platelet pseudopods were formed with adhesion and aggregation of platelets. 2. Deformation of erythrocytes. Triangle-like or shell-like cells, dacryocytes, twisted cells, and Ⅰ—Ⅲ type echinocytes were found. 3. Changes of endotheliocytes of the vascular wall became flattened. The space between these cells significantly widened (up to 4-18?m), and blood cells could be found to have wedged into it. 4. Formation of air-blood surface by the intravascular blood. 5. Alteration of organelies. There occured the expansion of rough endoplasmic reticulum, the degranulation and rupture of membranes, as well as the swelling and dissolution of mitochondria.

Objective To study the effect of hydroxychloroquine (HCQ) on the suppressors of cytokine signaling (SOCS) in the kidney of MRL/lpr mice and evaluate the protection of HCQ on lupus nephritis.Methods Twenty-six rats were randomly divided into 3 groups:the control group which was consisted of 6 mice; the MRL/lpr group which was consisted of 10 mice; and the HCQ group which was consisted of 10.24-hour urinary protein level and anti dsDNA levels were tested.The expressions of SOCS-1 and SOCS-3 protein were determined by western blot.Analysis of variance was used to determine statistically significant differences between the two groups.A P value less than 0.05 was considered to be statistically significant.Results Compared with the control group,the levels of 24-hour urinary protein [(2 356.6±509.3) mg vs (440.3±90.1)mg] and anti ds-DNA (128.7±32.3 vs 14.9±1.4) were significantly increased in the MRL/lpr group,the expression levels of SOCS-1 and SOCS-3 protein were increased.After treated with HCQ,the levels of 24-hour urinary protein [(70.8±11.1) mg vs (2 356.6±509.3) mg] and anti-dsDNA (111.8±330.1 vs 128.7±32.3)were significantly decreased,the expression level of SOCS-1 and SOCS-3 protein decreased significantly (P＜ 0.05).Conclusion HCQ can inhibit SOCS expression in the kidney of lupus mice,reduce proteinuria and delay progression of immune disorders and lupus nephritis.