Objective To investigate the low back pain(LBP)of clinic nurses and find out the understanding of related knowledge of LBP in nurses,and improve the occupational protection of nurses.Methods Investigation was carried out in 692 nurses by self-designed questionnaires.Results The incidence of LBP in nurses was 97.4%.The knowledge of LBP and the related occupational protection in nurses were greatly deficient.Conclusions The hospital should pay attention to the protection of LBP in nurses and improve their health.

Objective To investigate the leptin in continuous ambulatory peritoneal dialysis(CAPD)and its influen- cing factors.Methods 30 CAPD patients and 30 henhhy controls were enrolled in the study.Leptin was measured by ELISA.C reactive protein(CRP)by transmisson turbidmetry.Body mass index(BMI),fasting insulin(FINS), blood urea nitrogen(BUN),serum creatinine(Scr)etc were also determined.These patients were classified to two nutritional categories based on SGA normal nutrition and malnutrition.The relationship between serum leptin concentra- tion and these indices was analysed.Results Serum leptin levels in CAPD group was significantly higher than those of healthy control(P

OBJECTIVETo observe the clinical efficacy of Jiakangling Capsule (JC) combined with reduction of 1311 in treatment of Graves hyperthyroidism.

METHODSTotally 387 Graves hyperthyroidism patients were randomly assigned to the treatment group (200 cases) and the control group (187 cases). Patients in the treatment group took JC combined with reduction of 131I. The 131I dosage per gram of thyroid tissue was 50-80 microCi. They additionally took JC one week after taking 1311 for one consecutive month. Patients in the control group took 131 routinely as one disposable treatment. The 131I dosage per gram of thyroid tissue was 70-120 microCi, without using JC or other anti-thyroid drugs. All patients were reexamined after 24-month treatment. Whether hyperthyroidism was cured, incurred, or permanent was observed. Efficacies of thyroglobulin antibody (TGAb) and thyroid microsome antibody (TMAb) were compared between the two groups.

RESULTSCompared with the control group, the incurred ratio increased in the treatment group [3.2% (6/187) vs. 16.0% (32/200), P < 0.01], the incurred ratio of strong positive TGAb and TMAb patients increased [3.5% (2/57) vs. 27.1% (16/59), P < 0.01], the permanent hypothyroidism ratio decreased [21.1% (12/57) vs. 3.4% (2/59), P < 0.05 ].

CONCLUSIONJC combined with reduction of 1311 was superior in treating Graves hyperthyroidism induced permanent hypothyroidism than routine 1311 treatment, especially for strong positive TGAb and TMAb patients.

Autoantibodies ; Capsules ; Drugs, Chinese Herbal ; therapeutic use ; Graves Disease ; drug therapy ; Humans ; Hyperthyroidism ; drug therapy ; Hypothyroidism ; Iodine Radioisotopes

Objective To obtain the Tat-GFP fusion proteins with penetrating activity and labeled with green fluorescence protein (GFP), and to explore the cell membrane penetrating activity of Tat-GFP in MCF-7 cells. Methods The plasmid pET-24a-Tat-GFP was transformed into Escherichia coli BL21 cells. Different concentrations (0.5 and 1.0 mmol · L-1 ) of isopropyl-β-D-thiogalactopyranoside (IPTG ) and cell culture temperatures (22℃ and 37℃)were used to optimize the protein expression.The Tat-GFP proteins in supernatant were purified using Ni-IDA resins. Western blotting analysis was used to identify the Tat-GFP protein, and confocal laser scanning microscope (CLSM ) was used to examine the cell penetration of Tat-GFP protein. Results There was no significant difference in the Tat-GFP protein production induced by 0.5 and 1.0 mmol·L-1 IPTG;however,the low temperature (22℃)-induced BL21 cells expressed more Tat-GFP proteins than that at 37℃ induction.The Western blotting analysis results showed that GFP antibody could specifically recognize the proteins in PVDF membranes in dose-dependent manner;the CLSM results indicated the distribution of green fluorescence in cytoplasm and nucleus of MCF-7 cells.Conclusion The Tat-GFP protein highly expresses in the supenatant of Escherichia coli i BL2 1 cells at low temperature;the obtained Tat-GFP protein with green fluorescence preserves the cell penetrating activity.

Objective To study the differentiation of bone marrow mesenchymal stem cells in artery tissue under different calcification environments.Methods We made a vascular calcification model using warfarin,vitamin K1 and vitamin D3.After the model was successfully made,we took artery tissue of normal SD rat arteries and calcified arteries co-cultured with MSC,which were divided into three groups.The normal group included normal artery tissue with MSC; calcified inducers group included calcified inducers (dexamethasone with β-glycerophosphate and ascorbic acid),normal arterial tissue and MSC; calcification group included calcified artery tissue and MSC.Each group was cultured for 3 weeks.On the 10th day of the experiment,osteoprotegerin (OPG) protein secretion was detected by ELISA.After three weeks,changes of cell morphology were observed using inverted microscope,and total protein content and alkaline phosphatase (ALP) activity were detected by ELISA.In additional,the Ror2 (receptor tyrosine kinase-like orphan receptor 2) mRNA expression was detected by using RT-PCR method.Results MSC in calcification group spontaneously proliferated and differentiated to osteoblast-like cells.Compared with normal group,calcification group showed that the total protein content,ALP activity of bone metabolism markers,OPG were significantly elevated,while Ror2 mRNA expression was significantly decreased.MSCs in calcified inducers group did not differentiate to osteoblast-like cells,and the total protein content and OPG were increased,while ALP activity had no significant difference as compared with the normal group.However,Ror2 mRNA expression was lower in calcified inducers group than in normal group,while was higher than that in calcification group.Conclusions MSCs proliferate into bone-like differentiation in vascular calcification environment and aggravate the vascular calcification.And in normal vascular with calcified inducers environment,MSCs proliferate into smooth muscle cell differentiation and rehabilitate the vascular calcification.These phenomenons may be related to the Ror2 expression in artery.

Objective: To analyze the diagnostic methods and risk factors of female lung cancer, and to investigate the role of flberoptic bronchoscopy in the early diagnosis of lung cancer. Methods: The bronchoscopy data of 238 female lung cancer patients seen in our hospital between January 2005 and December 2008 were enrolled. The fiberoptic bronchoscop-ic features with clinical imaging, main risk factors of lung cancer and the pathological types were analyzed. χ~2 test was used for statistical analysis. Results: The major pathological type of lung cancer was adenocarcinoma in females younger than 40 years and squamous cell carcinoma in patients older than 60 years. The proliferative type was correlated with squa-mous cell carcinoma. Its imaging manifestion was a mass and the main risk factor was smoking. The infiltrating type was correlated with adenocarcinoma. Its imaging manifestation was mainly pleural effusion and the main risk factor was indoor soot inhalation. Conclusion: Females exposed to indoor soot inhalation for a long time especially those with a number of risk factors should be offered a screening with low dose CT (LDCT) regularly. Fiberoptic bronchoscopy and cytohistology are of great value for early diagnosis of lung cancer in those with abnormal findings in CT.

AIM: To investigate the relationship between somatostatin and the pathologic type, estrogen receptor,DNA ploidy of nuclei in tumor cells of breast cancer.METHODS: 67 cases of primary breast cancer and 25 cases of benign breast tumor were examined by immunohistochemical stretomyces avidin peroxidase method. 26 cases of breast cancer selected at random were analyzed by flow cytometry.RESULTS: Somatostatin expressed significantly higher in low malignant breast cancer than that in high malignant breast cancer ( P

Objective To analyze the pathological diagnosis of patients with clinically diagnosed eczema,to describe the spectrum of skin diseases tending to be misdiagnosed as eczema,and to investigate factors associated with their misdiagnosis.Methods A retrospective study was performed on 400 patients who were clinically diagnosed as eczema and received pathological examination at the dermatology clinic of Peking University Third Hospital from August 2006 to April 2013.Skin biopsy specimens were re-reviewed for these patients,and pathological diagnosis was made in combination with clinical presentations.Results Of the 400 outpatients with clinically diagnosed eczema,110 (27.5％) were finally diagnosed as non-eczema skin diseases pathologically,including 16 cases of psoriasis,13 bullous pemphigoid (BP),11 lichen planus (LP),9 cutaneous amyloidosis,8 mycosis fungoides (MF),14 skin malignancies,and 39 other skin diseases.The highest misdiagnosis rate was observed in people aged 60 to 79 years (33.9％) and lesions at the genital sites (46.2％).Conclusions Many conditions tend to be misdiagnosed as eczema in clinic,including psoriasis,BP,LP,cutaneous amyloidosis,MF and skin malignancies.Misdiagnosis is rather frequent in elderly people and eczematous lesions in genital areas,and pathological examination should be taken actively for uncertain cases.

Objective To assess the efficacy of cardiac resynchronization therapy (CRT) using real-time three-dimen?sional echocardiography (RT-3DE) and speckle tracking imaging (STI) in patients with heart failure. Methods Thirty-one patients with chronic heart failure were examined by RT-3DE and STI before CRT and 3, 6 months after CRT. The values of left ventricular end-diastolic volume (LVEDV), end-systolic volume (LVESV) and left ventricular ejection fraction (LVEF) were measured. RT-3DE was used to evaluate the minimum regional volume for 16 segments (Tmsv-16SD) and the ratio of Tmsv16SD to R-R interval, and the maximum time difference (Tmsv-16Dif). STl was used to evaluate the ratios of standard deviation of the time to peak longitudinal radial and circumferential strain to R-R interval (Tls-16SD%, Trs-16SD%,Tcs-16SD%) from the beginning of Q wave of electrocardiography to the point minimal systolic volume of all 16 segments and the maximum time difference. Results The values of LVEDV and LVESV were smaller 3 and 6 months after CRT than those before CRT. The value of LVEF was significantly improved after CRT (P < 0.05). The values of Tmsv-16SD%, Tmsv-16Dif%, Tls-16SD%, Trs-16SD%, Tcs-16SD%and T-Dif%were gradually decreased after CRT (P<0.05). The values of Tmsv-16SD%, Tmsv-16Dif%,Trs-16SD%and Tcs-16SD%were negatively related with LVEF (r=-0.79,-0.82,-0.69 and-0.74(P<0.05). The better consistency was found between RT-3DE and STI methods(Kappa=0.77). Conclusion Re?al-time three-dimensional echocardiography and speckle tracking imaging can provide more accurate quantitative informa?tion of cardiac resynchronization therapy for assessment of treatment and prognosis in patients with heart failure.

Tumor cell metastasis is a process of extracellular matrix hydrolyzed by protease, where tumor cells traverse the defect in the extracellular matrix into the lymphatic system and the capillaries to form new metastasis hematogenously.Matrix metalloproteinases (MMPs) are a kind of enzymes closely related to the metastasis.MMP, especially the relationship between MMP-9 and endometrial cancer metastasis in this paper will be summarized to get a better understanding about it.