Carcinoma, Hepatocellular ; complications ; Hemangioma ; complications ; diagnosis ; immunology ; pathology ; Hepatitis B, Chronic ; complications ; Hepatocytes ; cytology ; pathology ; Humans ; Liver Cirrhosis ; complications ; Liver Neoplasms ; complications ; Male ; Middle Aged ; Platelet Endothelial Cell Adhesion Molecule-1 ; immunology ; Spleen ; immunology ; pathology ; Tomography, X-Ray Computed

Animals ; Aspartame ; pharmacology ; Carbon Tetrachloride ; administration & dosage ; Disease Models, Animal ; Ethanol ; administration & dosage ; chemistry ; Liver ; pathology ; ultrastructure ; Liver Cirrhosis, Experimental ; chemically induced ; pathology ; Male ; Plant Oils ; administration & dosage ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Sweetening Agents ; pharmacology

OBJECTIVETo optimize extraction and purification methods of acidic polysaccharide from Moerella iridescens (MIAP).

METHODSWith alkali extraction process and orthogonal experiment,the time consumption,temperature,pH value of the solution and alcohol concentration during the extraction were optimized. The crude products were deprived of protein,pigment and ion,then were purified with DEAE-cellulose ion-exchange chromatography and verified with Sephadex G-100 and cellulose acetate membrane electrophoresis,and examined with infrared spectrum.

RESULTSThe optimized extraction conditions were as follows: extraction time 6 h,extraction temperature 70 degree,the solution pH 8.0 and the concentration of alcohol precipitation 70%. Intuitive features showed that the MIAP was pure white crystalline granular with slight dark brown color. The purification results demonstrated that the target MIAP was eluted and identified as a homogeneous components by DEAE-cellulose ion exchange column,Sephadex G-100 and cellulose acetate membrane electrophoresis. Infrared spectral scanning suggested that MIAP was α-D-type terminated glucopyranose. Intuitive features showed that MIAP was soft and cottony white.

CONCLUSIONThe extraction process with orthogonal test has been optimized and the acidic polysaccharide from Moerella iridescens is successfully isolated.

Animals ; Bivalvia ; chemistry ; Chromatography, DEAE-Cellulose ; methods ; Polysaccharides ; isolation & purification

Hepatitis B virus infection is a serious threat to human life and health. The approved anti-HBV drugs including interferons and nucleos(t)ide analogues have serious adverse effect, rebound phenomena after drug withdrawal, and drug resistance. And the cccDNA cannot be completely eliminated by both of them, which is the reason why a complete cure for hepatitis B cannot be achieved. Therefore, developing anti-HBV drugs directly targeting protein or nucleic acid of HBV remains a current public health priority. Based on the analysis of representative literature from the last decade, this article reviews recent developments in small molecule inhibitors directly targeting HBV from a medicinal chemistry perspective.

The direct acting substances of Cuscuta chinensis in vivo were preliminarily identified through the correlation analysis of "metabolites-effect identification" model. The ovariectomized female rats were i.g administered with 95% ethanol extract part, 40% ethanol elution part and n-butanol extract part of Cuscuta chinensis. The serum fingerprints of different parts and times of administration were established by UPLC/Q-TOF-MS. At the same time, serum estradiol (E₂), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels were detected. Bivariate correlation analysis and grey correlation analysis were used to screen estrogenic components. The results showed that nine direct acting substances in vivo highly related to estrogen effect were found in the drug containing serum, which were hyperoside, astragalin, methyl quercetin glucuronide, quercetin-diglucuronide, quercetin, apigenin, isoquercitrin, kaempferol glucuronide and kaempferol. We can preliminarily screen out the direct acting substance of estrogen effect of Cuscuta chinensis in vivo based on the research idea of serum spectrum effect correlation. It provides a reliable basis for revealing the estrogeneffective substances of Cuscuta chinensis and confirming the quality markers. This experiment was approved by Harbin University of Commerce Ethics Committees (Approval No. HSDU2020-065).

Objective To evaluate the two commercial cytomegalovirus IgG ELISA diagnostic kits.Methods Anti-CMV quality control panel QTC711 and seroconversion Panel PTC901 from BBI,seroconversion panel SCP-CMV-001 (RP-003) and SCP-CMV-002 (RP-019) from BIOMEX,and 2163 samples from three population groups were detect by the two kits.The inconsistent sample were retest by Diasorin CMV-IgG ELISA kit and Mikrogen recomblot CMV-IgG kit.Results Three seroconversion panel result show that the average detected positive time of A kit is 25 days earlier than B kit,the sensitivity of A kit is same as Abbott Imx CMV.The two kits detect 607 pregnant woman samples,8 were inconsistent,the coincidence rate is 98.68％; 512 outpatients samples,7 were inconsistent,the coincidence rate is 98.63％ ; 1044 Pediatric population samples,74 were inconsistent,the coincidence rate is 92.91％ ; the coincidence rate of Pediatric group is lower than other two groups.161 negative samples detect by A and B kits,and 89 samples positive by A kit but negative by B kit,were retest by Diasorin CMV-IgG ELISA kit,the positive coincidence rate of A and Diasorin kit is 100％,negative coincidence rate is 93.64％,total coincidence is 95.62％ ; the negative coincidence rate of B and Diasorin kit is 100％,total coincidence is 68.92％,78 samples were negative by B kit.12 A and Diasorin kit detect inconsistent samples and 6 consistent samples were retest by recomblot CMV-IgG,14 results were positive,4 results were negative.Conclusion The detection window period of A kit is shoter than B kit.The coincidence rate of A and B kit for pregnant women and outpatient population is higher than pediatrie population.The inconsistent samples were retest by imported kits,A kit show high consistent with imported kit than B kit.

Cell Line, Tumor ; Gene Expression ; Genetic Vectors ; Humans ; Lentivirus ; genetics ; Lipase ; genetics ; Membrane Proteins ; genetics

OBJECTIVETo evaluate the operation method and prevention from complications of extracranial carotid stenosis.

METHODSThree cases of carotid angioplasty and stenting for 271 patients with extracranial carotid stenosis were performed from October 2001 to June 2008. Before the operation, take Clopidogrel for 75 mg/d, Aspirin Delayed-Release Capsules for 100 200 mg/d, Simvastatin for 40 mg 1/night, for 5 - 10 d. Then treat continuous vein infusion Heparin 50 mg/d for 2 d. After the operation, continue antiplatelet and reduce blood fat therapy.

RESULTSAll 300 carotid stenting were successfully accomplished. DSA showed that the diameter of stenosed segment of carotid artery was markedly enlarged, and all clinical ischemia signs were improved remarkably. Seven cases suffered from complications in one week after operation and one died. Following up 3 - 24 months in 226 patients, restenosis were found in 5 cases, among which 45 patients were evaluated at follow-up by means of ultrasonic examination for 36 months or so, no restenosis was found. No ischemic attack occurred at follow-up.

CONCLUSIONSThe satisfactory effect and safety are achieved in the therapy of carotid stenosis by carotid angioplasty and stenting. Correct intraoperative treatment and skilled techniques are the key points of success.

Adult ; Aged ; Aged, 80 and over ; Angioplasty, Balloon ; Carotid Stenosis ; surgery ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Stents ; Treatment Outcome

OBJECTIVETo screen and verify differentially expressed genes in prostate cancer.

METHODSUsing DNA microarray, we screened differentially expressed genes in prostate cancer tissue and its adjacent tissue followed by verification by PCR.

RESULTSA total of 1 444 genes were found to be differentially expressed (differentiation ≥ 1.5-fold; P≤ 0.05) in the prostate cancer tissue, of which 769 (53%) were up-regulated and 675 (47%) down-regulated. Fifty percent of the differentially expressed genes showed a 1.5- to 2-fold differentiation, including 396 up-regulated and 182 down-regulated ones. Additionally, 308 up-regulated and 334 down-regulated genes exhibited a >2- to 5-fold, 46 up-regulated and 78 down-regulated genes a > 5- to 10-fold, and 19 up-regulated and 81 down-regulated genes a > 10-fold differentiation. Verification by subjecting 15 most significantly up-regulated and another 15 most markedly down-regulated genes to quantitative real-time PCR (qRT-PCR) showed that most of the genes had a transcriptional profile similar to that in the microarray data, with a Pearson correction coefficient of 0.83 between the microarray data and qRT-PCR results. Totally, 10 significantly differentially expressed genes were identified.

CONCLUSIONDNA microarray analysis provides reliable information on differentially expressed genes in prostate cancer and benign tissues. The 10 significantly differentially expressed genes verified by qRT-PCR could possibly become new bio-markers and specific molecules for tumor identification.

Cell Differentiation ; Down-Regulation ; Gene Expression ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; Oligonucleotide Array Sequence Analysis ; Polymerase Chain Reaction ; Prostatic Neoplasms ; genetics ; Transcriptional Activation ; Up-Regulation

OBJECTIVESTo evaluate the curative effect of embedding deep dorsal vein of penis on erectile dysfunction (ED).

METHODSThree patients with venous ED and 2 patients with mixed factors of artery and vein were treated by the embedding deep dorsal vein of penis. For evaluating curative effect the 5 patients were visited after operation.

RESULTSTwo months after surgery, the 3 patients of venous ED achieved satisfactory intercourse and the 2 patients with mixed factors of artery and vein had sufficient erection for intercourse after taking 50 mg Sildenafil. The 5 cases kept the above-mentioned curative effect during the follow up period between 3 to 12 months (7 months on avenage).

CONCLUSIONSThe embedding deep dorsal vein surgery, having small surgical wounds and few complications, is an effective treatment for venous ED.

Adult ; Humans ; Impotence, Vasculogenic ; surgery ; Male ; Penis ; blood supply ; surgery ; Veins ; surgery