Carcinoma, Hepatocellular ; pathology ; Diagnosis, Differential ; Humans ; Liver Cirrhosis ; pathology ; Liver Neoplasms ; pathology ; Precancerous Conditions ; pathology

Adenocarcinoma ; metabolism ; pathology ; Adenoma ; pathology ; Bile Duct Neoplasms ; metabolism ; pathology ; Bile Ducts, Intrahepatic ; CA-19-9 Antigen ; metabolism ; Cadherins ; metabolism ; Caroli Disease ; pathology ; Cholangiocarcinoma ; pathology ; Cystadenocarcinoma ; metabolism ; pathology ; Cystadenoma ; metabolism ; pathology ; Cysts ; pathology ; Diagnosis, Differential ; Hamartoma ; pathology ; Humans ; Keratin-19 ; metabolism ; Keratin-20 ; metabolism ; Keratin-7 ; metabolism ; Liver Diseases ; pathology

[Objective]To introduce a surgical technique used in the treatment of giant cell tumor of bone.[Method]Twenty-one patients were treated in our hospital from 1995 to 2003 for bone tumors of Campanacci Ⅰ~Ⅱ.All patients underwent curettage and "T" shape autologous bone transplantation and phenol.The patients were followed up for 18~24 months.[Result]Eighteen patients were healed on the basis of the duration,all knees presented no function limited.Two patients had local recurrence 8 to 10 months after the treatment.[Conclusion]For the giant cell tumor of bone around the knee,curettage and adjuvant "T" shape autologous bone transplantation is a good alternative method of reconstruction.

[Objective]To analyze the reasons of the missed and misdiagnosis of popliteal artery injury associated with knee dislocations, so that the patients can be diagnosed as early as possible.Amputation can be avoided.[Method]Twelve patients admitted in our hospital 1998～2003 who were misdiagnosed in other hospitals.Five patients had compound injured.The misdiagnosis time was 8～50 hours,meaned 22.6 hours.Five patients had an arteriogram after registes,one of them was recanalizated in radiology department.Other 6 patients were oqerated with saphenous vein bypass grafting,2 patients were anastomosed the end to the end and 3 patients were amputated directly.[Result]Three patients were healed completely,2 patients remained ischemic symptoms,4 patients were operated again with amputation.[Conclusion]There are four reasons for the missed and misdiagnosis:a.The doctors were not familiar with the anatomy of the popliteal artery;b.The knee was automatically reduction in the process of transmitting.There was no deformity when arriving at the hospital;c.The patients were severely injuried and the knee dislocations was ignored.d.The physical examination was not carefully done.

Caveolae are specialized lipid rafts that form flask-shaped invaginations of the plasma membrane. Many researches show that caveolae are involved in cell signaling and transport. Caveolin-1 is the major coat protein essential for the formation of caveolae. Recently, several reports indicated that the other caveolae-associated proteins, Cavins, are required for caveola formation and organization. It's worth noting that Cavin-1 could cooperate with Caveolin-1 to accommodate the structural integrity and function of caveolae. Here, we reviewed that the relationship between Cavins and Caveolins and explore the role of them in regulating caveolae.
Animals ; Caveolae ; physiology ; Caveolin 1 ; metabolism ; physiology ; Caveolins ; metabolism ; physiology ; Humans ; Membrane Proteins ; metabolism ; physiology ; RNA-Binding Proteins ; metabolism ; physiology

AIM:To investigate the construction of oligonucleotide microarray specialized for pancreatic adenocarcinoma-associated genes and its preliminary application of detecting differential expressed genes in pancreatic cancer.METHODS:Pancreatic cancer related genes were purposely selected,and oligonucleotide microarray was prepared by spotting oligonucleotide probes on glass slides coated with APS-PDC.Labeled cDNA targets for hybridizations were synthesized by reverse transcription from total RNA in the presence of Cy5-dCTP and Cy3-dCTP,respectively.Hybridized microarray was scanned by Agilent laser scanner,and the aquired image was analyzed by Imagene3.0 software.The intensity ratios of Cy3 and Cy5 were calculated.To confirm the expression profiles of these genes,quantitative reverse transcription-PCR (QRT-PCR) was carried out for CDC25B and TUSC3 genes,and β-actin gene was taken as internal control.The product of PCR was quantitated by comparative Ct method.RESULTS:The signal of microarray hybridization was clear,and the images had a lower background and higher signal-noise ratio.In comparison with normal pancreas,twenty -four differentially expressed genes were identified which included seventeen up-regulated and seven down-regulated genes.The results of QRT-PCR demonstrated that the expressions of CDC25B and TUSC3 in pancreatic cancer were up-regulated and down-regulated respectively,which is consistent with microarray results.CONCLUSION:The oligonucleotide microarray specialized for pancreatic cancer is desirable for its specialty and sensitivity,which can simultaneously and parallelly detect multiple pancreatic cancer-associated genes.

Animals ; Arrhythmias, Cardiac ; chemically induced ; prevention & control ; Cats ; Endothelin-1 ; adverse effects ; Medulla Oblongata ; drug effects ; Metoprolol ; pharmacology ; gamma-Aminobutyric Acid ; pharmacology

Humans ; Proteomics ; methods ; Silicosis ; metabolism ; Specimen Handling ; methods

Objective To investigate the position sense in chronic non-specific low back pain (CNLBP) patients, and the correlation to pain and endurance of psoas muscles. Methods From January, 2014 to May, 2015, 30 patients with CNLBP and other 30 healthy subjects were measured spine flexion and extension position sense absolute error (AE) and psoas endurance with Biodex System 3 synchronously, and the patients were self-assessed the pain in lumbar with Numerical Rating Scale (NRS). The Pearson correlation coefficient of AE to pso-as endurance and NRS was calculated. Results AE of flexion and extension was both more in the patients than in the healthy (t>1.862, P<0.05), and the psoas muscle endurance was less (t=2.307, P<0.05). AE of flexion (r=-0.71) and extension (r=-0.47) negatively correlated with the psoas muscle endurance in the patients, and positively correlated with NRS score (r=0.78 for flexion, and r=0.49 for extension). AE mildly negative correlated with the psoas muscle endurance (r=-0.38 for flexion, and r=-0.29 for extension). Conclusion Spine sensation has been impaired in CNLBP patients, and may associate with pain and psoas muscles endurance loss.