Objective To investigate the clinical values of preoperative through-the-scope(TST) stent implementation by analyzing its effectiveness and reliability in patients with acute colonic obstruction caused by proximal colonic cancer.Methods From June 2008 to June 2014,36 patients with proximal colonic cancer accompanied by acute obstruction were analyzed.Obstructive sites consisted of ileocecal junction(n =4),ascending colon(n =20),and transverse colon near the hepatic flexure(n =12).A-chievement ratio of the colonic tumor resection,the incidence rate of complications,operation time,and hospitalization were evaluated.Results TTS was successfully placed in all patients.All patients showed relief of obstructive symptoms within 1 ~2 days.Surgical resection was performed after 5 ~7 days.A total of 34 patients got a radical colonic tumor resection,while the other two patients had liver and pelvic metas-tasis.All patients were welly recovered without complications of anastomotic leakage,intraperitoneal infec-tion and bleeding.Conclusion For patients with acute colonic obstruction caused by proximal colonic cancer,preoperative through-the-scope(TST)stent implementation is a cost-effective and safe method.It reduces trauma and pain,and enhances life quality for patients,which is worthy of being spread.

Background Treatment of corneal ulceration by transplanting drug-loaded amniotic membrane has been used widespreadly abroad,however,seldom study is found in China up to now.Objective This study was to explore the sustained release property and the efficacy of the drug-loaded amniotic membrane.Methods The bacteriostatic area of amniotic membrane fragments immersed with different concentrations (5,20,30 g/L) of levofloxacin for different time points (5,15,30,60 minutes) was evaluated by in vitro test.Bacterial corneal ulceration models were established in 20 rabbits by injected 0.7 MCF staphylococcus aureus suspension (0.1 ml) into the central corneal stroma to form the cloudy area of 4.0-6.0 mm.Then the rabbits were randomized into two groups.Regular amniotic membrane transplantation was performed laterally and 0.5％ levofloxacin drops was topically administered after operation in the amnion+levofloxacin drops group,and drug-loading amnion transplantation was used in the drug-loading amnion group.Aqueous humor of 0.1 ml was collected in 30 minutes,1 hour,2,3.5,5.5 hours after levofloxacin was administered and 1 day,3,7,10,14,21 days after operation for the detect of levofloxacin level with high-performance liquid chromatography.The corneal symptom was scored based on McNeill's criteria in 1 week,2 weeks and 4 weeks and the ulceration area was assessed under the slit lamp in the first week.The pathological examination was carried out in the fourth week after surgery.Results The mean bacteriostatic area was bigger with the increase of levofloxacin concentration,and bacteriostatic area in amnion immersed for 15 minutes was bigger than that of 5 minutes (P＜0.01).The levofloxacin concentration of aqueous humor after transplantation was decreased by extending the time,and that in 30 minutes and 5.5 hours after operation was (0.873±0.264) mg/L and (0.106±0.027) mg/L,respectively,in the amnion+levofloxacin drops group,and that in day 1,3,7 after surgery in the drug-loading amnion group was higher than at 30 minutes in the amnion+levofloxacin drops group,showing all significant differences (all P =0.00).In the first,second and fourth week after operation,the corneal symptom score was 1.7±0.6,1.3±0.5,0.2±0.4 in the drug-loading amnion group and 2.2±0.8,2.0±0.6,1.5±0.8 in the amnion+ levofloxacin drops group,with the significant differences among the different groups and time points (Fgroup =9.49,P =0.01 ;Ftime =22.96,P=0.01).The corneal ulceration area was (1.6±0.6) mm2 in the drug-loading amnion group and (3.2±0.8) mm2 in the amnion+levofloxacin drops group 1 week after operation,showing a significant difference between them (t =3.98,P =0.00).Histopathological revealed that the various layers of cornea tissue appeared irregular arrangement in the amnion + levofloxacin drops group 4 weeks after operation with 1-2 layers of new squamous epithelium.Disorder hypothallus structure,more inflammatory cells and residual vascular cavity were visible.However,new squamous epithelium of 4-5 layers was seen in the drug-loading amnion group,and inflammatory cells and residual vascular cavity were less than the amnion+levofloxacin drops group 4 weeks after operation.Conclusions Levofloxacin-loaded amniotic membrane can sustained release levofloxacin and maintain an effective drug concentration in aqueous humor,which improves the treating efficacy for staphylococcus aureus corneal ulceration.

Objective To observe the effects of Qingzhi soft capsule(QSC) on improving experimental memory in mice.Methods The model of vascular dementia was made by means of bilateral carotid artery ligation and reperfusion after 8 minutes in mice.Another model of learn and memory disorder was induced by subcutaneous injection of sodium nitrite.The effects of Qingzhi soft capsule on memory disorder were evaluated by step-down test,Y-maze test in mice.Results Compared with sham-operated group,for the mice model of vascular dementia suffered cerebral ischemia and neural cell injury,the step down latency of animal model was shorter(P

Objective: To investigate the influence of the closed re-infusion of intestinal juice and EN on the treatment of intestinal fistula.Methods: The study group was composed of 21 patients of intestinal fistula from October 2004 to December 2006.The fistulae was in the 50 cm of the proximate jejunum and caused by trauma and operatin.The leak amount of intestinal juice was more than 1 000 mL.The protein level in serum and the absorption rates of the glucose,fat and nitrogen after the reinfusion of the intestinal juice were measured.Resutls: The protein level in serum and the absorption rates of the glusose,fat and nitrogen was increased after the re-infusion of the intestinal juice.Conclusion: The absorption of the glucose,fat and nitrogen in EN and the protein level in serum can be increased after the closed re-infusion of the intestinal juice.

Objective To examine the effect of father parenting stress and paternal marital satisfaction on father involvement,and to explore the mediating role of maternal perceptions of father involvement in father involvement and maternal marital satisfaction.Methods 300 children's fathers and mothers from 5 kindergartens in Beijing city,Dezhou city,Wuhu city were surveyed with the Chinese version of the PSI/SF,the father involvement questionnaire and the marital satisfaction questionnaire.Results The score of paternal marital satisfaction was (3.98±0.75),(3.84±0.76) for maternal marital satisfaction,(2.26±0.50) for father parenting stress,(2.60±0.49) for father involvement,(2.50±0.50) for maternal perceptions of father involvement.Correlation analysis showed that father parenting stress was negatively correlated with father involvement(r=-0.36,P＜0.01),paternal marital satisfaction was positively correlated with father involvement (r=0.33,P＜0.01),father involvement was positively correlated with maternal marital satisfaction(r=0.36,P ＜0.01),father involvement was positively correlated with maternal perceptions of father involvement (r=0.48,P＜0.01).A structure equation model consisting of father parenting stress,father involvement and marital satisfaction had a better degree of fitting:GFI =0.99,NFI =0.99,CFI =0.99,RMSEA＜0.001.Maternal perceptions of father involvement mediated the effect of father involvement on maternal marital satisfaction (effect value:0.41,P＜0.05).Conclusion Father parenting stress and paternal marital satisfaction could significantly predict father involvement,father involvement could significantly predict maternal marital satisfaction.These studies verified the spillover effect and the crossover effect between spousal subsystem and parental subsystem.

Objective To identify the member of the caspase family proteases involved in γ-radiation-induced apoptosis in HL-60 cells and to study the expression of the caspase gene in normal, apoptotic cells and in immortal tu mor cells. Methods By using degenerate oligonucleotide primers encoding the highly conserved peptides that were pre sent in all known caspases, we performed RT-PCR on poly(A)RNA from γ-radiation-induced apoptotic HL-60 cells. Caspase-3 mRNA in apoptotic HL-60 cells and in human tumor cell lines was analyzed by Northern blot. Results The amplified DNA fragment was identified with caspase-3 cDNA by cloning and sequencing. The Northern blot analysis of caspase-3 mRNA of different human tumor cell lines showed that the caspase-3 gene transcript was more highly ex pressed in leukemia cell lines and the SH-SY5Y cell line than in HeLa and MCF-7 cells. It was more highly expressed in the radiation-induced apoptotic HL-60 cells than in control HL-60 cells. Conclusion These results indicated that caspase-3 was involved in γ-radiation-induced apoptosis in HL-60 cells. The high level of expression of caspase-3 may aid efforts to understand the insensitivity of some tumor cells to radiation, their inherent ability to survive, and apop tosis.

A pot experiment was conducted to study effect of drought stress on leaf physiological characteristics and growth of one year old Stellaria dichotoma seedlings. The result showed that plant height and shoot dry weight significantly decreased with decrease in soil water content; however, root length and root dry weight increased at light drought stress and decreased at severe drought stress. The result also showed that with the decrease of soil water content, proline content in S. dichotoma leaves decreased then increase, while solube protein content decreased. Activities of SOD and POD in S. dichotoma leaves significantly decreased as soil water content decreased, while activity of CAT significantly decreased at severe drought stress. Membrane permeability in S. dichotoma leaves increased, while MDA content decreased then increased as soil water decreased. These results suggest that S. dichotoma had osmotic stress resistance ability and reactive oxygen scavenging capacity at light drought stress, which caused S. dichotoma growth was no inhibited at a certain extent drought stress.
Droughts ; Plant Leaves ; enzymology ; growth & development ; metabolism ; Plant Proteins ; metabolism ; Plant Roots ; enzymology ; growth & development ; metabolism ; Proline ; metabolism ; Seedlings ; enzymology ; growth & development ; metabolism ; Stellaria ; enzymology ; growth & development ; metabolism ; Water ; metabolism

To reveal the colonization characteristics in host of endophytic biocontrol bacteria NJ13 isolated from Panax ginseng, this study obtained the marked strain NJ13-R which was double antibiotic resistant to rifampicin and streptomycin through enhancing the method of inducing antibiotic. The colonization characteristics in ginseng and its biocontrol efficiency against Alternaria spot of ginseng in the field were studied. The results showed that the strain could colonize in root, stem and leaf of ginseng and the colonization amount was positive correlated with inoculation concentration. Meanwhile, the strain could infect and then transfer in different tissues of ginseng The colonization amount of strain in roots and leaves of ginseng increased first and then decreased. However, the tendency of colonization amount of strain in stems was ascend at first and then descend slowly, and was more than that in roots and leaves along with time, which had a preference to specific tissue of its host. In field experiment, the endophytic bacteria NJ13 was proved to be effective in controlling Alternaria leaf spot of ginseng. The biocontrol efficiency of fermentation broth at the concentration of 0.76 x 10(8) cfu x mL(-1) reached 75.62%, which was close to the controlling level (73.06%) of 0.67 mg x L(-1) 50% cyprodinil WG.
Alternaria ; physiology ; Antibiosis ; Bacillus ; growth & development ; isolation & purification ; physiology ; Endophytes ; growth & development ; isolation & purification ; physiology ; Panax ; growth & development ; microbiology ; Plant Diseases ; microbiology

Objective Set up virtual experimental teaching platform of medical molecular biology, and explore effective operating system of virtual experiment teaching. Methods 400 students of majored in clinical medicine in Kunming Medical University in Grade 2011 were randomly divided into the virtual experiment teaching group (n = 195 ) and the traditional experiment teaching group (n = 205 ). We realized the teaching effect by questionnaire survey, and analyzed the final exam results of two groups statistically. Results The experiment teaching way of virtual experiment has been widely accepted by students, and it could help students to understand and master experiment operations and theory knowledges.No statistical difference was found between two groups on the final exam. Conclusion Virtual experiment technology as a new teaching method has a lot of advantages, but it can't completely replace traditional experiments. We should use both the two kinds of teaching methods reasonally in the medical molecular biology experiment teaching.

Objective To determine the impact of PEE with different ethanol concentration extract on the activity of tyrosinase in B16 melanoma and to explore the feasibility of PEE used as skin whitening agent make use of a model organism .Methods 30%, 50%, 70%, 95%, different concentration of ethanol reflux got four kinds of PEE alcohol extract, respectively.And selected the most active inhibition to mushroom tyrosinase ( PEE30、PEE50、PEE70、PEE95).The effect of extracts from the selected on B16 cell model was determined by MTS, NaOH splitting decomposition, L-DOPA oxidation progress.The change in protein expression level after ethanol extract of PEE was determined by Western bolt.Results The capacity of inhibition to mushroom tyrosinase in each concentration of PEE extraction, but PEE70 had the most active inhibition.When challenged with B16 cell model, PEE70 showed the capacity of decrease in tyrosinase activities and melanin synthesis (P<0.01) and had a does dependence ;it also decreased Tyrosinase and TRP-1 expression compared with control B16 cells.Conclusion These data support the idea that PEE can restrain melanogenesis, through its inhibitory effect on the activity of tyrosinase.