This study is to investigate the effects of ceramide on GSTA1 expression in Caco-2 cells. After being exposed to ceramide for a fixed time, GSTA1 protein expression was detected by Western blotting analysis; GSTA1 mRNA expression was detected by real time PCR; dual luciferase assay was used to analyze GSTA1 transcriptional activity and GSTA1 activity was determined toward androstanedione (AD) as substrate. The data showed that ceramide can significantly induce the expression of protein and GSTA1 mRNA, and increase transcriptional activity and enzyme activity of GSTA1. The results demonstrated that ceramide may increase resistance to chemotherapeutics in Caco-2 cells by up-regulating the expression of GSTA1.

Resveratrol(RES)is a kind of antitoxin material produced by plant angainst fungi,it has many biological activites, which can restrain a variety of microorganisms.RES was reviewed in this paper with a variety of inhibition microbial func-tions in recent years,and summarized the antibacterial mechanisms of interfering with cell wall protein synthesis,cell mem-brane damage and RsbA mediating antibacterial factor formation.

Objective:To observe the clinical efficacy of acupuncture with medication for post-stroke depression.Methods:A total of 96 patients with post-stroke depression were randomized into an observation group and a control group,with 48 cases in each group.The control group was treated with oral fluoxetine hydrochloride capsules,and the observation group was treated with acupuncture on the basis of the treatment of the control group.After 6 months of treatment,the traditional Chinese medicine (TCM) symptom scores,and Hamilton depression scale (HAMD) component scores and total scores of the two groups before and after treatment were observed,and the clinical efficacy and adverse reaction rate were compared between the two groups.Results:The total effective rate was 95.8％ in the observation group and 83.3％ in the control group,and the difference between the two groups was statistically significant (P＜0.05).After treatment,the intra-group differences in TCM symptom scores and HAMD score of both groups were statistically significant (all P＜0.01).In the observation group,the scores of indifferent expression,emotional restlessness,and sentimentality in the TCM symptoms were statistically different from those in the control group (all P＜0.01).The scores of desperate factor and sleep disorder factor and total score in HAMD in the observation group were significantly different from those in the control group (all P＜0.01).The incidence of adverse reactions was 6.3％ in the observation group and 4.2％ in the control group,and the difference was not significant between the two groups (P＞0.05).Conclusion:Acupuncture plus medication is an effective method for post-stroke depression,and it can further improve the patient's bad mood.

Objective To investigate the thawing modes on stability of coagulation control products after frozen,looking for a new theoretical basis for cost control and the quality and safety of laboratory.Methods Using ACL TOP 700 automated co-agulation analyzer and supporting the same batch of reagents and quality control materials conduct of the study:after daily QC,recycled the remaining control materials immediately and dispensed into two EP tubes and frozen at-40℃,respectively thawed by room-temperature and 37℃water bath after 24 hours,and examined together with the date of quality control ma-terial,got 20 pairs of data for analysis the financial impact of two alternate ways on coagulation QC parameters.Results For the room-temperature thawing group,FIB high value increasedby an average 0.23 g/L (t=4.026 9,P<0.05);TT normal value average reduction of 0.46 s (t=-3.813 8,P<0.05),TT high value reduced by an average 0.41s (t=-3.972 8,P<0.05);D-Dimer low-value increased by an average of 14.75 ng/ml FEU (t=2.281 6,P<0.05),while APTT,PT normal and high value,FIB normal value,D-Dimer high value were no significant difference after thawing (P>0.05).For the 37℃water bath group,both normal and high value of APTT,PT,FIB,TT and D-Dimer were no significant difference after tha-wing (P>0.05).Conclusion The commercialization of coagulation control materials can be for the second QC,just follow the principle of rapid after melting and timely detection,other laboratories can be used as a reference.

Objective To investigate the diagnostic value of D-dimer age-adjusted threshold in elderly patients with Pulmonary thromboembolism(PTE).Methods Collected patients older than 50 years of suspected PTE,who visited Yan′an People′s Hospital and the Hospital Affiliated to Yan′an University from June 2015 to September 2016,using the revised Geneva criteria for clinical probability assessment firstly.The low-risk group was excluded from the study;the patients with moderate-to-high risk were performed D-dimer detection and CTPA.All patients determined both by D-dimer age-adjusted thresholds and traditional thresholds,comparing the diagnostic efficacy of the two methods subsequently.Results 163 patients were diagnosed with PTE by CTPA among the 549 subjects,the positive rate was 29.69％.The diagnostic sensitivity of plasma D-dimer was 83.44％,78.53％,the specificity was 17.88％,31.61％ respectively,the missed diagnosis rate was 16.56％,21.47％,respectively.The misdiagnosis rates were 82.12％ and 68.39％,respectively,and the Younden index was 0.013 2 and 0.101 4 respectively.All subjects were divided into four groups according to age:50-60 years,60-70 years,70-80 years,and equal or greater than 80 years age group.Compared with the traditional threshold,the misdiagnosis rates of the four groups of age-adjusted thresholds decreased by 4.00％,15.70％,21.36％ and 17.39％,respectively and the specificity was increased by 1.23,1.65,2.56 and 3.00 times,respectively.Conclusion The diagnostic accuracy of the age-corrected threshold is higher than the traditional threshold;combined with clinical practice,the optimal threshold is determined by the ROC curve,the clinician can serve as a reference.

OBJECTIVE:To observe the effect of Guben yangxue oral liquid on cyclophosphamide (CTX)-induced suppression of the immune and hematopoietic system in mice. METHODS:Immunosuppressive model mice were induced by intraperitoneal injection of CTX 50 mg?kg-1. Different dose groups were Guben yangxue oral liquid at the same time with Zhenqi fuzheng capsules as control. Several index such as peripheral blood cells, the weight of immune organ, serum hemolysin were determined. RESULTS:The levels of white blood cells (WBC,87.3、26.3、7.96 g?kg-1), lymphocytes (L,87.3 g?kg-1), neutrophils (N,87.3、26.3、7.97 g?kg-1), hemoglobin (Hb,87.3、26.3 g?kg-1), red blood cell (RBC,87.3、26.3 g?kg-1), platelets (PLT,87.3、26.3 g?kg-1), sp- leen index(87.3,26.3 g?kg-1), thymus index(87.3 g?kg-1), serum hemolysin (87.3、26.3、7.96 g?kg-1)in CTX-induced immunosuppressive mice were increased after administrated of Guben yangxue oral liquid. CONCLUSION:Guben yangxue oral liquid can enhance the non-specific immunity and humoral immune function of CTX-induced immunosuppressive mice, and it can improve CTX-induced hematopoietic dysfunction significantly.

Objective To study the antibacterial effect and mechanism of Resveratrol(RES)on Staphylococcus aureus stand-ard strains (ATCC 25923).Methods Using the microdilution protocol,to obtain minimal inhibitory concentrations(MIC)of RES on Staphylococcusaureus standard strains.Transmission electron microscope (TEM)and scanning electron microscope (SEM)were tested to obtain the effect of Staphylococcusaureus on Ultrastructure.Results The MIC of RES to Staphylo-coccusaureus standard strains ATCC 25923 were 0.256 mg/ml.Compared with control group,transmission electron and scanning electron microscope observation showed that after the act of RES on S.au standard strains,the cell morphology of S.au standard strains changed serious deformation,cells in all shapes and size,cell boundary roughness,cell wall defected and ruptured,and osteoporosis of edgeo,looseness of its structure,the nuclear membrane rupture,local showed vacuolar characteristics.Cell ultrastructure was obvious damaged and inhibition.Conclusion Rseveratrol had obvious inhibition and destruction to the standard strains of staphylococcus aureus,and the inhibition mechanism may be related to the destruction of cell membrane permeability.

ObjectiveTo investigate the clinicopathologic characteristics of different nation nonHodgkin lymphoma (NHL)in Urumqi of Xinjiang Uygur Autonomous Region.MethodsFour hundred and sixty-six cases of NHL were collected.Their sections including HE and immunohistochemical staining were examined again for diagnosis and classification.ResultsAmong 466 cases NHL,B cell neoplasm was 369cases (79.2 ％) and T cell lymphoma was 97 cases (20.8 ％).193 cases (41.4 ％) occured in the lymph node and 273 cases (58.6 ％) extranodal lymphoma.The most common subtypes were diffuse large B-cell lymphoma,small lymphocytic lymphoma/chronic lymphocytic leukaemia,extranodal marginal zone lymphoma of mucosaassociated lymphoid tissue,NK/T cell lymphoma,peripheral T-cell lymphoma,follicular lymphoma.Tlymphoblastic lymphoma (T-LBL) and anaplastic large cell lymphoma (ALCL) in the Uygur were higher than the Han [7.5 ％ (9/120) vs 1.3 ％ (4/308),x2 =11.276,P=0.001; 4.2 ％ (5/120) vs 2.3 ％ (7/308),x2 =1.137,P =0.286],and T/NK cell lymphoma in the Han was higher than the Uygur [7.1 (22/308) vs 3.3 ％ (4/120),x2 =2.196,P =0.138].There was no statistically significance between the Uygur and Han in different subtypes of B-NHL (P ＞0.05).ConclusionIn Urumqi aera,the incidence is higher in extranodal lymphoma than that in nodal.B-NHL is more in Urumqi area than that in other area in our country.There is no difference obviously for mobility B-NHL in the Uygur and Han,but T-LBL and ALCL in the Uygur are higher than those in the Han,and T/NK cell lymphoma in the Han is higher than that in the Uygur.It is suggested to confirm further whether the different associated with different nation and region.

Objective To investigate the effects of warming-needle acupuncture on the levels of matrix metalloproteinase-1 (MMP-1) and interleukin-1β (IL-1β) in the articular cartilage in knee osteoarthritis induced by immobilization in rabbits.MethodsA total of 30 adult male Japanese white rabbits were randomly assigned into three groups by random number table method: a normal group, a model group and a treatment group, with 10 rabbits in each group. Knee osteoarthritis was induced by 6 weeks of immobilization. The rabbits in the treatment group received warming-needle acupuncture after modeling at points of “Neixiyan” (EX-LE4), “Dubi” (ST35), “Zusanli” (ST36) and “Yanglingquan” (GB34) for 4 weeks. The level of MMP-1 and IL-1β in the articular cartilage of the knee were determined with radioimmunological assay.ResultsThere were significant difference in the levels of MMP-1(0.16 ± 0.02 ng/mg, 0.37 ± 0.02 ng/mg, and 0.28 ± 0.03 ng/mg;F=258.251) and IL-1β (0.21 ± 0.01 pg/mg, 0.34 ± 0.02pg/mg, and 0.31 ± 0.04 pg/mg;F=127.112) among the normal group, model group and treatment group. The levels of MMP-1 and IL-1β both in the model group and treatment group were significant higher than those in the normal group (allP<0.01), while the levels of MMP-1 and IL-1β in the treatment group were significant lower than those in the model group (allP<0.01).ConclusionWarming-needle acupuncture can effectively reduced the levels of MMP-1 and IL-1β in the articular cartilage in knee osteoarthritis induced by immobilization in rabbits.

Objective To explore prognostic factors of renal cell carcinoma and investigate the association of neutrophil-to-lymphocyte ratio (NLR) with the prognosis of renal cell carcinoma (RCC) in patients who received nephrectomy treatment.Methods We retrospectively reviewed the records of 1325 patients with renal cell carcinoma who underwent nephrectomy between January,2008 and December,2012.We retrospectively analyzed the clinicopathologic characteristics of patients.The optimal cutoff value for NLR was determined using receiver operating characteristic curve (ROC) analysis.We defined them as high NLR group when NLR ≥ 2.7 and low NLR group when NLR ＜ 2.7.Overall survival (OS) and recurrence free survival (RFS) were estimated using the Kaplan-Meier method and compared using the logrank test.Multivariate models were used to analyze the association of NLR with clinicopathologic outcomes.Results By the end of the study, 1220 cases were followed up.The follow-up rate was 92.1％.Mean follow-up was 40 months (range 2 months to 87 months).The three-year and five-year overall survival rate were 91.3％ and 86.9％, respectively.Meanwhile the three-year and five-year recurrence free survival rate were 88.2％ and 85.8％ ,respectively.2.7 was selected as the optimal cutoff value to differentiate between low NLR and high NLR.A NLR ≥2.7 was significantly associated with worse 5-year overall survival and worse 5-year recurrence free survival than a NLR ＜2.7,91.4％ vs 87.3％ ,89.6％ vs 71.9％ (P ＜0.05).Age ＞65, presentation mode with symptom, higher tumor stage, higher Fuhrman grade,histologic subtype,neutrophil count ≥ 4.5, lymphocyte count ＜ 1.7, NLR ≥ 2.7 significantly correlated with poor OS on univariate analysis.Multivariate analysis revealed that higher tumor stage, preoperative NLR ≥ 2.7 at diagnosis were poor independent prognostic factors for OS of renal cell carcinoma.Conclusion High NLR was independent poor predictor of renal cell carcinoma.