AIM:To investigate the effects of ursolic acid ( UA) on the migration and invasion of human lung cancer cell line A549, and to explore its mechanism .METHODS:The cell viability was detected by MTT assay .The ex-pression of miRNA-133a was detected in the A549 cells treated with UA by real-time PCR.The miRNA-133a mimics and inhibitor were transfected into the A 549 cells, and the transfection efficiency was analyzed by real-time PCR.The cell mi-gratory and invasive abilities were determined by wound healing and Transwell methods , respectively .RESULTS:The via-bility of the human lung cancer A549 cells was significantly inhibited by UA in a dose-dependent manner (P<0.05).IC50 of UA (24 h) for lung cancer A549 cells was 31.04 μmol/L.UA treatment significantly inhibited the migratory and inva-sive abilities of A549 cells in a concentration-dependent manner , accompanied by significantly elevation of miRNA-133a expression.The mimics and inhibitor of miRNA-133a significantly upregulated and downregulated the expression of miRNA -133a in the transfected A549 cells, respectively.In addition, the viability of the A549 cells was decreased extremely after tansfected with the miRNA-133a mimics (P<0.01), so did the results of the cell migration and invasion test .The A549 cells tansfected with the miRNA-133a inhibitor showed an opposite changes of the cell viability , migration and invasion . CONCLUSION:UA inhibited the viability , migration and invasion of lung cancer A 549 cells by elevating the expression of miRNA-133a.

A total of 450 residents from three neighborhood committees of Ba'nan were selected to assess their willingness to accept paid influenza vaccine and the influencing factors.Among them,273(60.7％) were willing to accept paid influenza vaccine.Age,economic status,health,flu recognition and vaccine approval were the major influencing factors of vaccination.

BACKGROUND:As a good candidate for bioresorbable bone graft, carbonate apatite monolith can be prepared by sintering procedure;however, sintering can cause carbonate loss and result in a much lower rate of
biodegradation compared to the human bone, thereby influencing the formation of new bone.
OBJECTIVE:To fabricate low-crystal inity carbonate apatite monolith as bone graft and test its properties. METHODS:Calcium carbonate monolith prepared by carbonation of Ca(OH) 2 monolith was treated in 1 mol/L
RESULTS AND CONCLUSION:The calcium carbonate completely transformed into low-crystal inity B-type
carbonate apatite after treated for 14 days. Diametral tensile strength of the final product was (10.27±1.08) MPa, which is adequate as a reconstruction material for bone defect. The carbonate content was (4.80±0.50)%, similar to that of the nature bone. The molar Ca/P was 1.63±0.01, indicating the Ca-deficient carbonate apatite is
obtained. The present method al ows an easy fabrication of low-crystal inity B-type carbonate apatite block with adequate strength and without sintering process.
ammonium dihydrogen phosphate solution at 60 ℃ up to 14 days. Diametral tensile strength was examined for biomechanical properties;X-ray diffraction analysis, Fourier transform infrared spectroscopy, scanning electron microscope observation and chemical analyses (carbonate, calcium and phosphate content) were also performed for physical and chemical properties.

In this study, we aim to investigate the protective effects of astragaloside on ultraviolet A (UVA)-induced photoaging in human fibroblasts and its possible mechanisms.

Apoptosis ; Child ; Heart Failure ; metabolism ; physiopathology ; Humans ; Signal Transduction ; Sympathetic Nervous System ; metabolism ; physiopathology ; Ventricular Remodeling

Objective To investigate the clinical value of Holter monitor(DCG) in the diagnosis and treatment of dilated cardiomyopathy (DCM).Methods According to NT-proBNP ＞ 2 000pg/mL,left ventricular ejection fraction (LVEF) ≤40％,New York Heart Association (NYHA) rated Ⅲ-Ⅳ,67 patients with DCM were divided into the high risk group and nonhigh risk group.All kinds of arrhythmia patients proportion was compared between the two groups,in order to understand the risk factors of DCM and arrhythmias.Results In high risk group,18 cases had ventricular tachycardia,the incidence rate was 66.7％,which was significantly higher than that of non high risk group (2 cases,5.0％) (x2 =29.271,P ＜ 0.01).While other arrhythmia,atrial premature beats,single premature ventricular contraction,atrial fibrillation and so on,had no statistical difference (P ＞ 0.05).Conclusion The DCM patients with various types of arrhythmia,ventricular tachycardia in patients directly influence the severity and prognosis,Holter monitor has an irreplaceable role.

Attention Deficit Disorder with Hyperactivity ; therapy ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Phytotherapy ; methods

Biomedical Research ; Humans ; MicroRNAs ; Neoplasm Invasiveness ; genetics ; Neoplasm Metastasis ; genetics

Bone Transplantation ; Humans ; Mandible ; transplantation ; Transplantation, Homologous

Objective To study the etiology,clinical presentation,MR images,treatment and prog-nosis of spontaneous spinal epidural and subdural hematoma.Methods From1995to2002,6cases diag-nosed as spontaneous spinal epidural and subdural hematoma were reviewed retrospectively.All of the pa-tients were males aging from30to68years with an average of 47years.The onset was acute while the pa-tients were in resting or light activity without prior history of any infection,administration of drugs or trau-ma.One patient had the history of hypertension,and another one was found to have diabetes after admission.The clinical characteristics were sudden pain involving neck,shoulder,chest and back,then dysfunction of motion and sensation of extremities and sphincter within few minutes.4cases of spontaneous spinal epidural hematoma were located at T 5~6 ,T 2~4 ,C 4~5 and C 6 ~T 1 respectively,and2cases of spontaneous spinal subdu ral hematoma at C 7 ~T 1 and C 7 ~T 2 respectively.Results All cases were proved with surgery and pathology.MR imaging showed occupying mass in the posterior or posteriolateral region inside the spinal canal.T 1 -weighted imaging was most useful owing to its typical signal changes with the hematoma.T 2 -weighted gra-dient echo sequence was capable to identify epidural and subdural spinal hematoma.Total laminectomy and hematoma debridement were performed.The outcome was closely related to the interval between the onset and surgery.The patients were followed up from6to18months with an average of 10months.All cases showed no significant improvement of spinal cord function except 5cases with incomplete recovery of sphincter function.Conclusion Spontaneous spinal epidural and subdural hematoma are rare entities.MRI is a most helpful diagnostic tool for this disorder.It should be stressed that early diagnosis and prompt surgical intervention are of great importance for prognosis of spontaneous spinal epidural hematoma.