1.Analysis on tigecycline and commonly used anti-bacterial agents resistance in Gram-positive cocci and Acinetobacter baumannii
Gaoli CHEN ; Lihua XIN ; Ling YANG
International Journal of Laboratory Medicine 2014;(9):1143-1144,1163
Objective To understand tigecycline and commonly used anti-bacterial agents resistance in Gram-positive cocci and Acinetobacter baumannii .Methods 445 strains of Gram-positive cocci and 83 strains of Acinetobacter baumannii were collected . VITEK 2 Compact automated bacterial identification and susceptibility analysis system was employed to identify Gram-positive coc-ci and Acinetobacter baumannii and their bacterial sensitivities toward tigecycline and commonly used anti-bacterial agents were de-tected by broth microdilution method for antimicrobial susceptibility testing (MIC assay) and Kirby-Bauer disk diffusion susceptibil-ity test(K-B) ,respectively .Results 155(34 .8% ) strains of Staphylococcus aureus ,78(17 .5% ) Enterococcus feces ,56(12 .6% ) Staphylococcus haemolyticus ,45(10 .1% ) Staphylococcus epidermidis ,43(9 .7% ) Enterococcus faecalis ,18(4 .1% ) human Staphy-lococcus ,16(3 .6% ) Wolfowitz Staphylococcus ,8(1 .8% ) birds Enterococci and 26(5 .8% )other strains were found in 445 cases of Gram-positive cocci .The rate of penicillin-resistant Staphylococci was 92 .4% and the rates of Staphylococci′s resistance to eryth-romycin ,clindamycin ,oxacillin and ciprofloxacin were all over 50% .Enterococci ,which was sensitive to linezolid and tigecycline ,was resistant naturally to many drugs .The tigecycline sensitive rate ,intermediate rate and resistance rate acquired by MIC assay for multi-drug resistant and pan-resistant Acinetobacter baumannii were 57 .1% (47/83) ,42 .9% (36/83) and 0 .0% (0/83) ,respec-tively ,while those by K-B methods were 2 .4% (2/83) ,40 .5% (34/83) and 57 .1% (47/83) ,respectively .Conclusion Tigecycline showes good antibacterial activity toward Gram -positive cocci and Acinetobacter baumannii .
3.Expression of HLA-G and IL-10 in patients with acute leukemia.
Jin YANG ; Shao-Lin ZHAO ; Xin-Ling YANG
Chinese Journal of Hematology 2012;33(2):135-137
Acute Disease
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Adolescent
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Adult
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Aged
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Child
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Female
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HLA-G Antigens
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metabolism
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Humans
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Interleukin-10
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metabolism
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Leukemia
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metabolism
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Male
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Middle Aged
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Young Adult
5.Prospective study of the Perigee system in the treatment of anterior pelvic organ prolapse
Qiuying LAI ; Xin YANG ; Ye ZHU ; Chen TAN ; Mei LING
Chinese Journal of Obstetrics and Gynecology 2016;(2):103-108
Objective To evaluate the short-term efficacy of Perigee system in the treatment of anterior pelvic organ prolapse. Methods From October 2012 to September 2014, 59 patients with pelvic organ prolapse, pelvic organ prolapse quantitation system (POP-Q) were diagnosed as anterior pelvic organ prolapse Ⅲ degree and above were performed Perigee anterior pelvic floor reconstruction, while some patients combined with sacrospinous ligament suspension, posterior wall repair or posterior pelvic reconstruction surgery for pelvic prolapses. Pelvic floor distress inventory-short form 20 (PFDI-20), pelvic organ prolapse-urinary incontinence sexual questionnaire-12 (PISQ-12) were evaluated, and postoperative POP-Q were used to analyze the changes of the indexes and postoperative complications. Results In 59 patients, the average operation time was (99±29) minutes, the average intraoperative blood loss was (119± 92) ml. The median postoperative follow-up time of 59 cases was 17.5 months (range:8-30 months), median follow-up time of subjuctive symptoms was 21.2 months (range:11-34 months), the total score of PFDI-20 was compared with the preoperative, and the difference was statistically significant (5.6 versus 27.8, P<0.01). It was statistically significant of PISQ-12 score before and after surgery (34±3 versus 36±4, P<0.05). Short-term anatomical cure rate was 98%(58/59), 1 cases (2%, 1/59) in recurrence, 2 cases (3%, 2/59) of erosion. Conclusion This results show that the Perigee system is effective and reliable in the treatment of anterior pelvic organ prolapse.
6.Data-mining of clinical treatment of hypertensive disease by professor Zhou Yixuan
Mei DONG ; Ling XIN ; Fan YANG ; Xiaohua DAI
International Journal of Traditional Chinese Medicine 2015;(2):165-168
Objective To investigate the clinical experience and scientific theory for treating hypertension by Zhou Yixuan. Methods The clinical records of 149 patients with hypertension treated by Zhou Yixuan were input into the database, and the terms were standardized. The Chinese medical diagnosis , therapeutic principles, prescriptions and Chinese herbs were analyzed. Results The syndromes of 149 patients with hypertension appeared frequently in the phlegm dampness, qi deficiency and blood stasis, qi and yin deficiency, hyperactivity of liver-yang, liver and kidney yin deficiency. The prescriptions appeared frequently in Shenling-Baizhu powder, Buyang-Huanwu decoction Shengmai powder, Tianma-Gouteng Yin, Liuwei-Dihuang pill. The Chinese herbs appeared frequently in honey-fried milkvetch root, finger citron, Szechwan lovage rhizome, danshen root, Chinese angelica, fructus corni, white peony alba, red peony root, prepared largehead atractylodes rhizome, spine date seed, orange fruit, coix seed, tangshen, silktree albizia bark, and leech. Conclusions The syndromes of hypertension appeared in deficiency complicated with excess. Qi deficiency, qi and yin deficiency, liver and kidney yin deficiency were deficiency syndromes. Hyperactivity of liver-yang, blood stasis and phlegm were empirical syndromes. Zhou Yixuan was good at treating hypertension with the methods of benefiting vital energy, nourishing yin, regulating vital energy, promoting blood circulation, reducing phlegm and calming the nerves.
7.Clinical observation on treating severe neonatal diaper rash with hip bath and oxygen blowing
Liqing YANG ; Ling HE ; Qin YUAN ; Jiao NIE ; Ping XIN
Chinese Journal of Practical Nursing 2013;(2):19-20
Objective This research is to observe the treatment effects of hip bath and oxygen blowing on severe neonatal diaper rash.Methods 289 neonates with severe diaper rash were randomly divided into three groups.91 cases in the flat tube group,102 cases in the round tube group and 96 cases in the control group.Three groups of neonates were cleaned on the hips and perineuma after poops and then dried with wet tissues and Mupirocin Ointment.The control group was treated with the above-mentioned method.The oxygen blowing groups were treated with hip bath of 1:5000 chameleon solution twice a day.Blowing the hips with oxygen five minutes every time after hip bath.The method of blowing oxygen was that oxygen humidifying containers as normal oxygen aspiration facilities was not filled with water,whose oxygen flowing volume standed at 10 L/min and whose tube blew at the afflicted parts until being dry.The oxygen blower held the oxygen exit and blowed at the afflicted parts in the round tube group and the oxygen blower flattened the oxygen exit and blew at the afflicted parts in the fiat tube group.The treatment effects will be compared among the three groups four days later.Results The cure period of the round tube group was obviously shorter than that of the control group,and the cure period of the fiat tube group was remarkably shorter than that of the round tube group.The total effective rate in the round tube group was obviously higher than that of the control group and the total effective rate in the fiat tube group was obviously higher than that in the round tube group.The difference had a statistical significance.Conclusions The treatment effects for the severe neonatal diaper rash with hip bath and oxygen blowing are remarkable and the oxygen blowing effects with fiat tubes are better than those with round tubes.
8.Obsevsation on the effect of docetaxel combined with 5-fluorouracil and cisplatin in the treatment of patients with advanced gastric cancer
Ling ZHAO ; Ming HUANG ; Yinshan YANG ; Hua TAN ; Xin ZHAO
Chinese Journal of Primary Medicine and Pharmacy 2013;20(7):971-973
Objective To evaluate the efficacy and adverse effects of docetaxel combined with 5-fluorouracil in treating patients with advanced gastrice cancer.Methods 130 patients with advanced gastric cancer were randomly divided into observation group and control group,observation group treated with docetaxel combined with 5-fluorouracil,the control group treated with 5-fluorouracil and cisplatin,the clinical efficacy after the treatment was observed.Results The clinical efficiency rate of the observation group was significantly higher than that of the control group,the rate of leukopenia,thrombocytopenia and gastrointestinal reactions of the observation group was significantly lower than the control group,the quality of life scores of the two groups after treatment were all improved,but that of the observation group was significantly higher than the control group,the difference was statistically significant(P < 0.05).Conclusion Docetaxel combined with 5-fluorouracil was effective in treating advanced gastric cancer.It worth further clinical research and application.
9.Effect of Shengmai injection combined with norepinephrine on apoptosis of lym-phocyte and apoptosis protein in MODS rats
Ling YANG ; Chunyuan YIN ; Xin Lü ; Guichen ZHANG
Chinese Journal of Immunology 2016;32(8):1133-1136,1140
Objective:To study the effect of the Shengmai injection combined with norepinephrine on cellular immunity in rats with multiple organ dysfunction syndrome .Methods:40 healthy male rats were randomly divided into the control group ,the Shengmai injection group ,the norepinephrine group and the Shengmai injection combined with norepinephrine group of 10 rats in each group after MODS rat model were established by intraperitoneal injection of zymosan .The plasma level of TNF-α, IL-1β, IL-12 and IL-2 were detected by ELISA ,and the protein expression of caspase-3,caspase-9 and caspase-8 in spleen were tested by IHC during 6 h,24 h,48 h.And the lymphocyte number of apoptosis and the ratio of Th 1/Th2 were tested by flow cytometry.Results: ① Compared with the control group, the plasma concentrations of TNF and IL-1βwere not significant increased in the Shengmai injection group , the norepinephrine group and the Shengmai injection combined with norepinephrine group ,as the extension of treatment time ,the plasma concentration of TNF and IL-1βwere significant lower in treatment groups than control group .While the plasma concentration of IL-12 and IL-2 were significantly increased in treatment groups , and the combined treatment group was more obvious .② Compared with control group,the protein expression of caspase-3,caspase-9 and caspase-8 in spleen were decreased in the Shengmai injection group , the norepinephrine group and the Shengmai injection combined with norepinephrine group ,and the combined treatment group was more obvious.③The lymphocyte number of positive apoptosis were significantly decreased , and the Th1/Th2 ratio were increased in the Shengmai injection group ,the norepinephrine group and the Shengmai injection combined with norepinephrine group ,and the combined treatment group was more obvious .Conclusion: The Shengmai injection combined with norepinephrine can reduce the inflammatory reaction and improve the immune function in the rat with multiple organ dysfunction .
10.Traditional Dispensing Mode vs. Automatic Oral Drug Dispensing Mode in Single Dose Administration System
Jianhua SONG ; Yang WANG ; Yin LI ; Ling TAN ; Xin HU
China Pharmacy 1991;0(04):-
OBJECTIVE:To evaluate the merits and demerits of automatic oral drug dispensing mode as compared with traditional dispensing mode.METHODS:The medication data in our hospital after adoption of single dose administration system were collected and summarized analytically in respect of drug quality management.RESULTS:The automatic oral drug dispensing mode is superior to traditional dispensing mode.CONCLUSION:Under single dose administration system,the application of automatic oral drug dispensing mode is conductive to the improving of pharmaceutical care level.However,the problems existing in its clinical use remain to be further studied.