BACKGROUND:An animal model of myocardial infarction is of great significance for the study of the pathogenesis, pathophysiology and evaluation of treatment methods of human coronary artery disease. Many researchers aim to improve survival rate and the success rate of model induction.
OBJECTIVE:To improve the method of making a rat model of myocardial infarction.
METHODS:One hundred Wistar rats were divided into model group (n=80) and sham-operated group (n=20). The rats were intraperitoneal y anesthetized with 3%sodium pentobarbital. Tracheostomy was operated. An automatic breathing apparatus was utilized for assisted breathing. A left anterior thoracotomy was performed at the fourth intercostal space. The left anterior descending coronary artery was ligated. Sham-operated rats underwent similar surgery but without coronary artery ligation. The thoracic cavity was closed layer by layer. The breathing apparatus was removed and tracheal intubation was also removed. To prevent suffocation, the trachea and the neck incision were not closed.
RESULTS AND CONCLUSION:Heart morphology and hematoxylin-eosin staining demonstrated that the success rate of establishing myocardial infarction model was 98.6%. The survival rate of model group at 3 weeks after surgery was 88.75%. Total y nine rats died, including seven dead rats during operation and within 24 hours after operation, and two dead rats in later stage. The survival rate of the sham-operated group was 100%. We modified the method of establishing a rat model of myocardial infarction and improved the operation success rate and survival rate of rats, such as anesthesia method, longitudinal incision during tracheotomy, without closing the trachea after operation, and the method of opening and closing the chest.

Objective To evaluate the similarity of dissolution profiles of self-produced and original drug cefuroxime axetil tab-lets.Methods Based on the dissolution detection method in the Chinese Pharmacopoeia,the dissolution behaviors of the self-pro-duced preparation and original drug were investigated in pure water,0.1 mol/L hydrochloric acid,0.05 mol/L hydrochloric acid, pH4.5 and pH6.8 medium and the rotation speed of 50,25,75 r/mim.The stripping curves were evaluated by the f2 factor method. Results In the rotation speed of 50 r/min,the f2 factors in different mediums were 57.65,79.17,73.56,66.83 and 62.33 respec-tively;the medium was 0.1 mol/L hydrochloric acid with the rotation speed of 25,75 r/min,the f2 factors were 65.35 and 78.48 re-spectively.Conclusion The stripping curves of self-produced preparation and original drug are similar under various conditions.

Objective To analyze the clinical methods of type Ⅲ Pilon fracture internal fixation. Methods The internal fixation with steel plate were performed in 36 cases with Ruedi-AllgoWer type, Ⅲ Pilon fracture and all patients had early post-operative rehabilitation functional exercise. Results Thirty-six cases were followed up for an average period of 16 months. The fracture healed completely in all cases and the successful rates of good function reached 88.9% (32/36) according to YU Guang-rong's evaluation standard. Conclusion For Ruedi-AUgower type Ⅲ Pilon fracture, appropriate surgical time combined with internal fixation with steel plate and early post-operative function exercise showed satisfactory therapeutic effect.

Books ; Language ; Linguistics ; Medicine, Chinese Traditional ; Translations

The role of bacterial biofilms in the chronic diseases has been recognized more and more. The experts in the centers for disease control and prevention of the United States estimate that 65% of human bacterial infection is involved with the biofilms. In the recent study. it has been proved that there is a high correlation between the bacterial biofilm and the pathogenesis and prognosis of chronic rhinosinusitis(CRS). In order to elucidate the role of bacteria films in the pathogenesis of CRS, we reviewed various method of testing the bacterial biofilms in CRS, and summarized the related researches. Based on the detection researches and summaries, we conclude that bacterial biofilm can contribute to the continuance and development (diffusion and repeated) of CRS. It can lead to the continuous inflammation by influencing the immune system of the mucous membrane. While there is little research on the bacterial biofilm. Further researches will be needed for the exact mechanism of the bacterial biofilm in CRS. in order to find more effective therapeutic method and targets.
Biofilms ; Chronic Disease ; Humans ; Sinusitis ; microbiology ; pathology

OBJECTIVE: Explore the TGF-beta1 and collagen fibers in chronic nasal sinusitis each type and degree of expression of the normal control group and in the nasal mucosa epithelial tissue remodeling and the role of TGF-beta1 and collagen deposition relationship. METHOD: Sixty-two patients experimental group (CRSwNP of 21 cases. CRSs NP group of 15 cases. 11 cases of recurrent nasal polyps; control group 15 cases specimens for immunohistochemistry and masson collagen staining. Manual counting the number of positive cells by the Mann-Whitney U test to analyze the expression in experimental group and the control group. Experimental group and control group between the single-factor analysis of variance with a One-Way ANOVA analysis. RESULT: Experimental group and control group were expressed TGF-beta1 with collagen deposition. Which, TGF-beta1 in CRSsNP group was significantly lower than the control group (P < 0.05), CRSwNP group was significantly increased compared with CRSsNP group (P < 0.05); masson collagen staining, CRSsNP group was significantly lower than the control group (P < 0.01), recurrent nasal polyps group than in the control group was significantly increased (P < 0.05). TGF-beta1 and collagen staining masson positive correlation between (P < 0.01 ). CONCLUSION TGF-beta1 and collagen deposi tion and chronic nasal-sinusitis tissue remodeling has its relevance. Furthermore. TGF-beta1 expression increased with excessive deposition of collagen fibers also positively correlated.
Adult ; Case-Control Studies ; Chronic Disease ; Collagen ; metabolism ; Humans ; Middle Aged ; Nasal Mucosa ; metabolism ; Rhinitis ; metabolism ; pathology ; Sinusitis ; metabolism ; pathology ; Transforming Growth Factor beta1 ; metabolism ; Young Adult

Objective To describe the morphological characteristics of foveal retinoschisis and retinal detachment in highly myopic eyes. Design Retrospective observational case series. Participants Twenty-nine patients (38 eyes) of high myopia with foveal retinoschisis and retinal detachment. Methods All patients with foveal retinoschisis and retinal detachment were performed complete ophthalmic examinations, B-scan and studied cross-sectional images of the maculae with optical coherence tomography (OCT). 10 eyes underwent vitreous surgery. Main Outcome Measures Morphological characteristics. Results On biomicroscopy, the maculae of all 38 eyes had a microcystic appearance and shallow elevation without macular hole. B-scan showed posterior retinal edema or with 0.5 to 2mm detachment. OCT disclosed foveal retinoschisis and retinal detachment in all eyes. The neuroretina splitted into a thick inner layer and a thin outer layer or a thin inner layer and a thick outer layer or a thin inner layer, a thick inter layer and a thin outer layer. A hyperreflective preretinal structure resembling the posterior hyaloid was stretched over the retinoschisis and drew the retina. Vitreous surgery performed on 10 eyes, including posterior vitreous detachment, removal of the premacular vitreous cortex. In all eyes, foveal detachment and retinoschisis gradually decreased in height, and attained foveal attachment finally. Conclusion Foveal retinoschisis and retinal detachment in highly myopic eyes with staphyloma is better characterized with OCT than with biomicroscopy. Vitreomacular traction forms the posterior hyaloid and the staphyloma maybe the major cause of it.

Objective To study the influence of diabetes on the CK activity in different tissues of streptozotocin induced diabetic rats.Methods Serum samples,heart and skeletal muscles,brain and bladder tissues were collected from both streptozocin induced diabetic rats and control group.CK was measured by enzymatic method.Results The body weight,heart weight,and brain weight reduced significantly compared with control group(P

Radionuclide imaging was widely used in the diagnosis and treatment of skeletal diseases.It is of early diagnostic value in metastatic osteosarcoma，osteomyelitis and Paget's disease,and early affirmative value in vitality of transplanted bone.It also demonstrates a significant analgesic effect on theosteosarcoma and metastatic osteosarcoma.

Objective To study the expression of PTEN,Fas and DCR3 in human gastric cancer and evaluate their effects on carcinogenesis,cell proliferation,invasion and metastasis of gastric carcinoma,as well as clinical significance.Methods The expression of PTEN,Fas and DCR3 were detected by immunohistochemical method (streptavidin-peroxidase) in the tissues of 75 primary gastric carcinomas and 15 gastric ulcers.Results The positive expression rate of DCR3 in gastric carcinoma tissues was significantly higher than that in normal tissues,and the expression of PTEN and Fas was obviously lower than that in normal tissues (P<0.01).The expression of DCR3 was negatively correlated with Fas and PTEN respectively (r=-0.720,P<0.001; r=-0.336,P<0.001).There was a positive correlation between PTEN and Fas.The expression of PTEN,Fas and DCR3 was closely correlated with lymph node metastasis,clinical stage,histological differentiation grade and tumor depth of gastric cancer,but not related to sex,age and tumor size (P>0.05).Conclusion PTEN,DCR3 and Fas have mutual restrictive relationships in the process of invasion and metastasis in gastric cancer.Detecting the expression of PTEN,Fas and DCR3 in gastric cancer is helpful to judge the malignant biological behavior of gastric cancer at a different angle,which may be considered as reliability index in clinic diagnosis,judging curative effect and monitoring the prognosis of gastric carcinoma.