Objective To investigate the drug resistance of Acinetobacter baumannii strains isolated during 2014 in a hospital of Beijing.Methods Isolated bacteria were cultured by routine method,identified and performed drug susceptibility test by bacteria a-nalysis system.Statistical analysis of Acinetobacter baumannii was conducted by Whonet5.6 software.Results A total of 226 Acin-etobacter baumannii strains were isolated from January 2014 to December 2014 in Fengtai Teaching Hospital of Capital Medical U-niversity.These strains showed the lowest resistance rates to minocycline and Cefoperazone-sulbactam(23.0% and 30.1%).The other antimicrobial resistance rates were more than 50.0%.The resistance rate of Acinetobacter baumannii varied from one depart-ment to another.Pandrug-resistant and multi-drug resistant Acinetobacter baumannii were 71.2%,1 9.0% respectively. Conclusion The resistant rates of Acinetobacter baumannii strains to a variety of antibiotics are high.It is essential to strengthen monitoring the drug resistance of Acinetobacter baumannii,as well as use antibiotics reasonable and separate patients to control Acinetobacter baumannii infection.

Action Potentials ; Animals ; Female ; Heart ; physiology ; Male ; Membrane Transport Proteins ; genetics ; Mice ; Mice, Knockout ; Myocardium ; metabolism ; Patch-Clamp Techniques ; Sodium Channels ; physiology

Adult ; Aged ; Asteraceae ; Drugs, Chinese Herbal ; adverse effects ; Female ; Hepatic Veno-Occlusive Disease ; chemically induced ; Humans ; Male ; Middle Aged ; Retrospective Studies

Background Research determined that hypoxia inducible factor-1(HIF-1)is associated with the hypoxia response in normal organ,and it plays an important role during the embryon development.It is also proved that the expression of vascular endothelial growth factor(VEGF)is upregulated in embryon retina.But,whether the action of HIF-1 and VEGF is correlated is unclear. Objective The aim of this study was to investigate the dynamic change of expression of HIF-1α and VEGF in retina during embryonic development and explore the role of HIF-1α and VEGF along with retinal evolvement.Methods The different embryon ages of SD rats were obtained from 30 clean pregnant SD rats by cesarean surgery.The retinas were isolated from embryon 10一day,12一day,14-day,16-day and 20-day rats respectively.The expressions of HIF-1 α and VEGF protein in retina were semi-quantitatively and qualitatively determined by immunochemistry,and the expressions of HIF-l α and VEGF mRNA in retina with different-embryon-phase and adult rats were detected by RT-PCR. Results The hishly level of expression of HIF-1 α and VEGF protein were found in the cellular nuclei and cytoplasm of retina in embryon 10一and 12-day rats.With the increase of embryon age.the expression of HIF-1α and VEGF protein in retina lowed(F=56.70,P<0.01；F=60.78.P<0.01).Compared with the adult rats,the expressions of HIF-1α and VEGF protein in retina were higher from embryon 0-day through 20-day rats(all P<0.01).The significantly positive correlation was found in the expression level between HIF-1α and VEGF protein throughout the experimental duration(r=0.96,P=0.00).Followed the same pattern,the expression levels of HIF-1α and VEGF mRNA in retinas were also enhanced in the embryon 10- and 12-day rats.Identically,a gradually weakened trend was seen in the expressions of HIF-1 α and VEGF mRNA in retinas as the increase of gestational age of rats(F=68.84,P<0.01；F=96.49,P<0.01),and the exDressions of HIF-1 α and VEGF mRNA decreased in the adult rats compared with different embryon-phase rats,showing a statistically significant difference between them(all P<0.01). Conclusion The expressions of HIF-1 αand VEGF in retina appear a dynamic alteration from hish to low during the embryonic development of rat retina,indicating that HIF-1α/VEGF pathway might participate in the process of embryo development of rat retina.

Objective To investigate the familial incidence of multiple primary carcinoma in Chinese hereditary nonpolyposis colorectal cancer patients (HNPCC) in Northeast China.Methods By family line investigation,multiple primary carcinoma (MPC) spectrum' s characteristics of 509 patients in 85 families registered in strict conformity with the HNPCC Amsterdam criteria Ⅱ were analyzed retrospectively.Results Of the 85 HNPCC families,multiple primary carcinoma developed in 55 patients in 25 families,among them 45 patients had metachronous carcinoma in 17 families,16 patients had synchronous carcinoma occurred in 12 families,6 patients with both synchronous carcinoma and metachronous carcinoma in 4 families.Conclusions Multiple primary carcinoma developed in significantly high incidence in Northeast China in Chinese hereditary nonpolyposis colorectal cancer patients,the most common MPC are colorectal cancer and endometrial cancer.

Objective:To observe the effect of enriched rehabilitation training on cognitive function, plasma mir-146a-5p microRNA precursor levels and inflammatory factors in persons with post-stroke cognitive impairment (PSCI).Methods:Fifty-eight persons with PSCI were randomly divided into an observation group and a control group, each of 29. The observation group was given enriched rehabilitation training, while the control group was provided with conventional cognitive rehabilitation training. The Montreal Cognitive Assessment Scale (MoCA), the Digit Span Test (DST), parts A and B of the Trail Making Test (TMT A-B) and the Modified Barthel Index (MBI) were used to assess the subjects′ cognitive functioning and their ability in the activities of daily living (ADL). Plasma levels of mir-146a-5p, IL-6 and TNF-α were detected before and after the treatment.Results:After treatment, the average MOCA, DST and MBI scores, as well as the average TMT A-B times had improved significantly for both groups. However, the observation group′s averages were significantly better than those of the control group on all three tests. After the treatment, the average plasma expression of miR-146a-5p had increased significantly in both groups, but the increase in the observation group was significantly greater. Plasma IL-6 and TNF-α levels were significantly lower than before the treatment, with the average TNF-α level in the observation group significantly lower than that of the control group.Conclusions:Enriched rehabilitation training can improve the cognition of stroke survivors more effectively than conventional cognitive rehabilitation training. That may be related to the up-regulation of plasma miR-146a-5p and reducing inflammation.

Objective To explore the short?term functional outcomes of the reconstruction of the proximal humerus by re?verse shoulder arthroplasty after tumor rescetion. Methods 8 patients who underwent reverse shoulder arthroplasty after tumor resection between January 2013 and December 2014. 5 were female and 3 were male, mean aged was 38 years old (25-61). 2 chon?drosarcomas and 6 giant cell tumors. Enneking stageing of 2 cases with chondrosarcoma were stageⅠB and stageⅡB. 6 giant cell tumors were Campanacci stage 3, meanwhile 3 cases had pathological fractures. The deltoid and axillary nerve were intact in all patients by image analysis before the operation. The proximal humerus was resected according to Malawer typeⅠresection. Then reverse shoulder arthroplasty reconstruction and bone graft was performed. The follow?up was scheduled, and the patient received X?ray examination of the shoulder. The range of motion of the shoulder was measured, the Constant?Murley score and musculoskel?etal tumor society(MSTS) score was recorded. Results The mean duration of the operation was 2.7 h (2-3.5 h). The bleeding in the operation was 510 ml (300-850 ml). The mean length of humerus resection was 8 cm (6-10 cm). The allografts were used in 7 cases and reimplantation after tumor bone deactivation was used in one. The latissimus dorsi transfer were performed in 2 cases. The rotator cuff were resected 1-1.5 cm from the great and lesser tubercles. The follow?up was 13 months (3-26 months). No infec?tion, dislocation, or loosening of prosthesis was found by the last follow?up. The X?ray showed the case who received reimplanta?tion after tumor bone deactivation had achieved bone union 1 year postoperation,7 cases received allograft had still nonunion at the host?graft junction. Bone resorption were found in all cases in different extent but the prosthesis were stable. No local recur?rence of the tumor was found. At last follow?up, active abduction was 155° (100°-175° ) and active forward elevation was 150° (115°-170°) and Constant?Murley score was 76%(68%-87%). The MSTS score was 92%(87%-97%). Conclusion The func?tional outcomes of the reconstruction of the proximal humerus by reverse shoulder arthroplasty after tumor rescetion was satisfied in early period. The reverse shoulder arthroplasty can be used in younger patient, but long?term results need further study.

Objective To evaluate the effects of different reconstruction settings of knowledge-based iterative reconstruction technique (IMR) on noise reduction and image quality in hepatic contrast-enhanced CT(CECT) at different radiation dose levels.Methods Patients who underwent hepatic CECT because of their diseases were enrolled in this prospective study.According to random number table,patients were randomly assigned to two groups (standard dose group,SD-group,n=29; low dose group,LD-group,n=25).All patients underwent both non-enhanced CT and triphasic CECT scan including hepatic arterial phase (HAP),portal-venous phase (PVP) and delayed phase.PVP images of SD-group were acquired with tube voltage of 120 kVp and tube current-time products of 250 mAs,and 80 kVp and 500 mAs for LD-group.PVP images were reconstructed with both filtered back projection (FBP) and IMR techniques.Settings applied in IMR reconstructions consisted of body routine (BR) and body soft tissue (BST) with three levels (L1 to L3),and image series included:L1BR,L2BR,L3BR,L1BST,L2BST and L3BST.Subjective assessment of image quality including low contrast detectability (LCD),image distortion (ID) and diagnostic confidence (DC) as well as objective image quality including image noise,signal to noise ratio (SNR) and contrast to noise ratio (CNR) were compared between groups.Effective radiation dose was recorded.Objective image quality and subjective image quality were compared by using Friedman H test and ANOVA,respectively.In addition,Student t test was used to compare effective radiation doses between groups.Results In SD-group,IMR settings of L2BST,L3BST scored highest in LCD with median score of 4;L3BST and L2BST scored lowest in ID with median score of 3,while FBP and L1BR scored highest with median score of 4; L1BR,L2BR and L1BST scored highest in DC with median score of 4,while L3BST scored lowest with median score of 3.In LD-group,the distribution of all reconstruction settings scores in LCD and ID were similar to those in SD-group; however,L1BST scored highest with median score of 4,and FBP scored lowest with median score of 2 in DC.There were statistical differences in LCD,ID and DC among all the reconstruction settings for both groups (P＜0.05).In terms of FBP technique,the image noise,SNR and CNR were (9.8±2.0)HU,13.3±3.3 and 6.0±1.9 in SD-group,and (16.2±4.1)HU,12.9±3.3 and 6.6± 2.3 in LD-group,respectively.In SD-group,image noise exhibited a trend of decrease from L1BR to L3BST [from (4.5±0.9) HU to (3.2±0.2)HU],while SNR,CNR trended to increase (SNR:from 21.4±1.4 to 24.6±4.6; CNR:from 9.4±2.2 to 10.9±2.9,respectively).The image noise,SNR and CNR in LD-group showed the same trends as SD-group [image noise:from (5.8±0.2)HU to (3.9±0.6)HU; SNR:from 26.3±4.5 to 33.1±6.9; CNR:from 13.6±4.0 to17.4±5.1,respectively].In both groups,there were statistically differences in image noise,SNR and CNR among all IMR settings and FBP (F values were 15.50 to 131.39,P＜0.01).The effective radiation dose of the LD-group was significantly lower than that of the SD-group [(4.19±0.22) versus (7.32±0.58) mSy,t=15.27,P＜0.01).Conclusions IMR can reduce image noise and improve image quality in hepatic CECT at both standard and low dose levels.L1BR,L2BR and L1BST are the most optimized reconstruction settings for the standard dose protocol,while L1BST performs best for the low dose protocol.

To design a military hospital bonus accounting system. The system used Java as the fore-ground development tool and SQL Server 2005 as the background database, which extracted data from the military hospi-tal cost accounting management system to calculate the bonus with some formula. The system had four functional modules to complete department benefit accounting, bonus calculation, bonus summarization and bonus inquiry. The system decreases the workload and contributes to the standardized management of the accounting.

Objective To investigate the effects of heat-killed Staphylococcus aureus (HKSA) on the apoptosis and expression of iNOS and IL-1β in THP-1 monocytes in the presence of high concentration of glucose.Methods THP-1 cells were cultured in medium containing 25.0 mmol/L(HG) or 5.5 mmol/L (LG,control) D-glucose for 12 h-8 d.The THP-1 cells cultured for 6 d were extracted on the 0-48 h with or without HKSA,then apoptosis and expression of iNOS and IL-1β were examined.Apoptosis was analyzed by flow cytometry and expressions of IL-1β and iNOS were quantitated by real-time PCR.Results The expression of iNOS and IL-1β in THP-1 monocytes was increased significantly in the presence of high concentration of glucose for 12-48 h(P＜0.05),reaching the highest level at 24 h and returned to baseline after 4 d.The expression was significantly lower than that of control after 4-6 d.Apoptosis rate was also increased significantly after 48 h to 4 days.HKSA infection enhanced apoptosis,but inhibited the expression of iNOS and IL-1 β in the presence of high concentration of glucose.The expression of iNOS and IL-1β increased significantly at 6 h(P＜0.01),reaching the highest level at 12 h,but the levels were significantly lower than those in control groups (P＜0.05).Conclusion These data suggest that high concentration of glucose can interfere with the anti-bacterial function of monocytes by reducing their expression of iNOS and IL-1β and enhancing their apoptosis.