Objective To investigate the effect of amyloid on the expression of kinesin superfamily(KIFs) genes in cortex of Wistar rats. Methods The animal model of Alzheimer's disease was prepared by injection of amyloid into brain ventricle of rats.The learning impairments were verified by water maze test.The levels of mRNA for six kinesin superfamily genes,kinesin,KIFlb,KIFlc,KIF3a,KIF3c,and KIF4,in cortex of rats,were estimated by real-time RT-PCR. Results Two weeks after amyloid injection,the rats showed significant learning impairments.In cortex,the expression of KIF genes was increased after amyloid injection.Conclusion\ The increase of KIF gene may be an evidence for the excitotoxity of amyloid to neurons.

Objective To investigate the effect of isochlorogenic acid B(ICAB)on CCl4-induced acute liver injury(ALI)in mice. Methods The animal model of CCl4-induced ALI in mice was established and then the protective effect of ICAB was evaluated using this model. Serum levels of alanine transaminase(ALT)and aspartate aminotransferase(AST),hepatic levels of malondialdehyde (MDA)and the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were measured by spectrophotometric methods. Liver cell morphology was observed by hematoxylin and eosin staining method and the effects of ICAB on the protein expres-sion of nuclear factor erythroid-2-related factor 2(Nrf2),heme oxygenase-1(HO-1)and quinone oxidoreductase 1(NQO1)in mice he-patocyte were determined by Western blot method. Results ICAB(5,10 and 20 mg/kg)significantly protected against CCl4-induced liver injury by reducing the elevated levels of serum aminotransferases and hepatic MDA and remarkably restored the impaired antioxi-dants. Meanwhile,the histopathological changes were also attenuated in mice. In addition,ICAB could induce the protein expression of Nrf2 and promote its nuclear translocation,and further increase the protein expression of HO-1 and NQO1. Conclusion ICAB has protective effect against CCl4-induced ALI in mice,which is mainly due to its ability to promote the nuclear translocation of Nrf2 and decrease the oxidative stress.

BACKGROUND: The denervated skeletal muscle is chiefly characteristic as the loss of neurotrophic factor, which may cause the muscle atrophy degeneration and fibrosis. OBJECTIVE: To investigate the effect on the muscle satellite cells and muscle atrophy by the implantation of basic fibroblast growth factor (bFGF) released through silicon tube in the gastrocnemius. DESIGN, TIME AND SETTING: Randomly control animal experiment was finished at the Animal Experiment Laboratory of Shenyang Medical College Fengtian Hospital in November 2006. MATERIALS: Twenty-eight Wistar healthy male rats (weighing 250-300 g) were adopted. Silicon tube was prepared by the encapsulation with bFGF or physiological saline, and sealed with 502 glue. METHODS: The sciatic nerve of left lower limb was cut off, and then gastrocnemius was taken out. All of 28 Wistar rats were divided into experiment group and control group randomly, each group involved 14 rats. The experimental gastrocnemius was encapsulated with silicon tube containing bFGF, while the control group with physiological saline. MAIN OUTCOME MEASURES: At day 30 postoperatively, the following indicators were evaluated: wet weight of gastrocnemius and wave amplitude of muscle fibrillation potential; proliferating cell nuclear antigen positive nuclei on surface of muscle fibers, diameter and section area of gastrocnemius fiber under light microscope; ultrastructure of gastrocnemius under electron microscope. RESULTS: The number of muscle satellite cell nuclei in the experimental group was more than that in the control group (P

On the basis of the theories of air sterilization, the Virobuster steritubes are very effective in the removal and inactivation of airborne bacteria species, viral particles, fungi and spore by using the high-intensity UV lamp and close-in UVC treatment room with high reflecting sputtering aluminum, the sterilization efficiency were above 99.999%～99.999 9% when the atmosphere passed through the germicidal tubes. As a technical breakthrough, the Virobuster steritubes could overcome the problems of traditional air sterilization and decontamination technology, the air sterilization could reach LOG-6 sterilizing effect. By analyzing of the workings principles and structure characteristics of Virobuster steritube, the optimization design of air sterilization and decontamination is set up for our public buildings air and hospital sterile room.

The incidence rate of nonalcoholic fatty liver disease (NAFLD) has been increasing in China in recent years and has reached 11％-15％.At present,the multiple-hit hypothesis is considered the main pathogenesis of NAFLD,yet the specific pathogenesis remains unknown.This article elaborates on the roles of carcinoembryonic antigen-related cell adhesion molecule 1 highly expressed in the liver,vitamin D/vitamin D receptor axis,and psychological factors in the development and progression of NAFLD,in order to provide new directions for the research on the pathogenesis of NAFLD in future.

The data of autism in online question and answer communities of Zhidao.baidu.com and Yahoo!Answers were analyzed, which showed that the understanding of online question and answer communities users in China and USA was quite different.The users in USA mastered the basic knowledge of diseases much better than those in China and more detailed questions were asked by users in USA than by those in China.The clarity, completeness, accuracy and operability are the most important factors for selection of answers.Autism and its countermeasures have been well and widely accepted in American society.

Objective:To establish a method for the determination of chloride and sulfate in lithium carbonate crude drug by sup-pressed ion chromatography. Methods:The analysis column and the guard column was Thermo IonPac AS11(250 mm × 4 mm) and IonPac AG 11(50 mm ×4 mm), respectively. Potassium hydroxide solution(10 mmol·L-1) generated by an eluent generator(EG) was used as the eluent at a flow rate of 1. 0 ml·min-1 . And the temperature of column and conductivity cell was 30℃ and 35℃, re-spectively. The suppressor electric current was 36 mA, and the injection volume was 10 μl. Results:The linear range of chloride ion and sulfate ion was 1. 0-20μg·ml-1 with r of 0. 995 1 and 0. 997 3,respectively, and the recovery was 106. 6%(RSD=2. 6%,n=6) and 100. 3%(RSD=1. 9%,n=6), respectively. Conclusion:The method is accurate and reliable, and can be used in the determina-tion of chloride and sulfate in lithium carbonate crude drug.

Objectives To provide outline for the Research & Development department of hospitals making research strategy by analyzing the quantity of publications and their cited status for the top 20 hospitals in SCI and MEDLINE, and the top 100 hospitals in domestic publications in 2008. Methods The number of publications and their cited times for the first 20 hospitals in SCI and MEDLINE, and the top 100hospitals in domestic publications in 2008 were analyzed with bibliometric analysis. Results Compared with 2007, the increased number and ratio of publications in SCI and MEDLINE for the top 20 hospitals were 1050, 51.19% and 658, 15.04%, respectively; for top 100 hospitals, the average decreased number of domestic publications was 2.08, and the number of cited publications was increased 202.52 and the increased ratio was 13.51%. Conclusions The SCI publications from top 20 hospitals increase sharply, the increase of the MEDLINE articles from the top 20 hospitals is slowly. The negative growth is showed in the number of domestic articles for the top 100 hospitals. In addition, the increase of cited articles is slowly and disproportion.

Objective To explore the expression of cell apoptosis regulatory elements in esophageal canceration course. Methods 60 specimens of esophageal carcinoma and corresponding adjacent no cancerous tissue were detected quantitatively by propidium iodide(PI) stain and indirect immunofluorescence of fluorescein isothiocyanate(FITC) using flow cytometry(FCM) equipment. Results Expression of bcl-2 and c-FLIP in esophageal carcinoma was significantly higher, and Fadd, Caspase-8 and Caspase-3 were significantly lower than that in adjacent tissues (P <0.01). There was positive correlation between Fadd and Caspase-8 gene (P <0.01 or P <0.05). The expression of Fadd was positively correlated to Caspase-8. Comparing with normal epithelium and dysplasia tissue, DNA content and heteroploid cells were increased significantly in esophageal carcinoma. Conclusion During the esophageal canceration course, expressions of relevant cell apoptosis regulatory elements were abnormal. DNA content and heteroploid cells were increased significantly in esophageal carcinoma.

Objective To investigate the authors, the institution and endemic distribution of these authors of Chinese Journal of Orthopaedics, and understand the current situation and problems in the field of Orthopaedics. Methods The authors, the co-authors and the core authors of papers published in Chinese Journal of Orthopedics from 2005 to 2009 were quantitatively analyzed using literature search through CMCI.The distribution of institution, region and highly quantitative organization were also analyzed. Results The 1182 papers were published by the Journal within 5 years. There were 1108 papers with one or more co-authors; the cooperative rate and degree were 93.74% and 5.02, respectively. 74 papers were written by single author, which accounted for 6.26% of all papers. 615 authors (75.18% of all first authors) published only one paper. There were 295 (24.96%) papers from 67 (8.19%) core authors. 601 (50.85%) papers were from 20 high-productivity institutes. The top three high-productivity institutes were Tianjin hospital, Beijing Jishuitan hospital and Nanjing Drum Tower hospital of Nanjing University Medical School. The number of papers were 89 (7.53%), 75 (6.35%) and 43 (3.64%), respectively. The number of papers from the teaching hospital,provincial or municipal hospitals, military hospitals were 722 (61.08%), 226 (19.12%) and 157 (13.28%),respectively. According to the distribution of Province, Beijing was the first of place with 264 (22.34%) papers, followed by Shanghai by 160 (13.54%) papers and Tianjin by 136 (11.51%) papers. The distribution range of the authors has been expanded to five countries. Conclusion The influence of the Chinese Journal of Orthopaedics has greatly increased. Authors of the Chinese Journal of Orthopaedics have a highly cooperative rate. There is a group of active and talented core authors who has a great influence on the journal. But the number of core authors and the papers published by core authors need to be expanded.