Ocular ischemic syndrome(OIS) is a disease seen in cardiology,ophthalmology,neurology and neurosurgery,which is characteristic by brain and ocular symptoms caused by carotid artery obstruction or stenosis.The diagnosis of OIS may be difficult because of its variable presentations and its concealed onset.What is more,its pathogenesis is not completely clear,and therefore its treating efficacy is dissatisfactory so far.The current study on OIS is focused on experimental research.So establishment of suitable animal model is important.Recently years,the creating method of OIS model made a great progress.The selection of model animals,extablishing methods of models and comparison of various models were summarized.

Background Fungal keratitis can cause serious damage to visual function of corneal infective disease,which is more difficult to treat.In recent years,injecting antifungal drugs to the corneal stroma not only enrich the treatment of the disease,but also achieve good clinical effects.Correctly selecting drug kinds and drug concentration can improve the cure rate,and reduce adverse reactions after treatment,but the related research is rare.Objective This study was to observe the clinical effects of corneal stroma fluconazole injection with different concentration for the treatment of fungal keratitis.Methods Prospective study was performed.One hundred and two patients (102 eyes) diagnosed as fungal keratitis were included from May 2012 to January 2015 in Jizhong Energy Xingtai Mining General Hospital.The patients were randomly divided into 3 groups,The eyedrop treatment group (29 eyes) received 0.5％ fluconazole eyedrops and 5％ natamycin eyedrops treatment.The 0.1％ fluconazole group (35 eyes) and 0.2％ fluconazole group (38 eyes) received 0.1％ and 0.2％ fluconazole corneal stroma injection after eyedrop treatment,respectively.Each group underwent potassium hydroxide wet examination and fungal cultures.The curative effect and adverse reactions were observed.Results Fusarium 41.2％ (42/102),Aspergillus 21.6％ (22/102)and Alternaria mold 17.6％ (18/102) ranked the top three pathogenic species.The distribution of pathogenic fungus among the 3 groups were significantly different (x2 =3.763,P＞0.05).The cure rate of eyedrop treatment group was 44.8％ (13/49),which was significantly lower than 0.1％ fluconazole group (74.3％,26/35) and 0.2％ fluconazole group (81.6％,31/38) (x2 =5.782,9.854;both at P＜0.05).The cure rate was significantly different between 0.1％ fluconazole group and 0.2％ fluconazole group (x2=0.566,P＞0.05).The average cure time of eyedrop treatment group,0.1％ fluconazole group and 0.2％ fluconazole group were (36.28 ± 10.39),(29.14± 7.86) and (21.34 ± 8.57) days,respectively,with a significant difference among the three groups (F =5.336,P=0.006).The acuity of vision was significantly increased after treatment in the 0.1％ fluconazole group and 0.2％ fluconazole group (t =3.009,4.695;both at P ＜ 0.01).The average number of injection in the 0.1％ fluconazole group was (5.71 ± 2.97) times,which was higher than (5.13 ± 1.80) times in the 0.2％ fluconazole group (t=4.471,P＜0.05).Four cases in 0.2％ fluconazole group with eye irritation were observed.After diclofenac sodium eyedrops treatment,the symptoms of 3 cases were disappeared.One case was cured by conjunctival flap covering method.All the cases were followed up for 1-3 months,no adverse reactions and recurrence was found.Conclusions Corneal stroma fluconazole injection is an effective method for treatment of fungal keratitis.In order to reduce the occurrence of adverse reactions,0.1％ fluconazole injection in corneal stroma is recommend for light fungal keratitis patients;for moderate and heavy fungal keratitis patients,0.2％ fluconazole injection in corneal stroma can be performed after failure of 0.1％ fluconazole treatment.

Objective:To find a better way to protect the security and interests of vulnerable groups by exploring the problem of protecting the vulnerable groups in drug clinical trial from the perspectives of ethics committees,organization and researchers.Methods:According to the relevant literature and the actual situation of the hospital,this paper analyzed the security issues of vulnerable groups comprehensively.Results:Only the ethics committees,organization and researchers work together,can it protect the security and interests of vulnerable groups to the greatest extents.Conclusion:Further research on the security of vulnerable groups not only promotes the development of human health,but also plays a decisive role in improving the protection of subjects in drug clinical trial.

Objective:To study the expression of IQ motif containing GTPase activating protein 1 (IQGAP1 )in oral squamous cell cancer(OSCC)tissue,and to explore the effects of IQGAP1 on cell proliferation and invasion as well as its underlying mechanism. Methods:Expression levels of IQGAP1 in tumor and adjacent normal tissues were examined by western blot and RT-PCR.OSCC cell line SCC-4 cells was transfected with the recombinant plasmid-pcDNA3.1 -IQGAP1 by lipofectamine,and then treated with an Akt in-hibitor.The phosphorylation of Akt,cell proliferation and invasion were detected by western blot,MTT assay and Transwell invasion as-say respectively.Results:Protein and mRNA expression levels of IQGAP1 were higher in cancer tissue than in adjacent normal tissue (P <0.05).Transfection of pcDNA3.1 -IQGAP1 increased IQGAP1 expression,enhanced the capability of cell proliferation and inva-sion (P <0.05),increased p Akt level in the cells.Preconditioning with an Akt inhibitor reduced p Akt level.Furthermore,silencing Akt pathway blocked the increase of cell proliferation and invasion induced by IQGAP1 overexpression(P <0.05).Conclusion:IQ-GAP1 overexprission can mediate the ability of proliferation and invasion of OSCC cells by regulating the activation of Akt pathway.

Objective To investigate the low back pain(LBP)of clinic nurses and find out the understanding of related knowledge of LBP in nurses,and improve the occupational protection of nurses.Methods Investigation was carried out in 692 nurses by self-designed questionnaires.Results The incidence of LBP in nurses was 97.4%.The knowledge of LBP and the related occupational protection in nurses were greatly deficient.Conclusions The hospital should pay attention to the protection of LBP in nurses and improve their health.

Biomarkers ; Hepatitis B ; therapy ; virology ; Hepatitis B Surface Antigens ; Hepatitis B virus ; Humans

Teaching quality is the basis of higher education.This article compared the results of teaching supervision and evaluation between campus and affiliated hospitals of Qiqihar Medical University in 2011.Differences were found out and corresponding strategies were proposed in order to improve the teaching quality and train high-quality talents.

Circulating microparticles (MPs) derive from a variety of cells,including platelets,leukocytes,endothelial cells and erythrocytes.In addition,tumor cells release MPs into blood.MPs are formed as a result of membrane lipid remodeling and proteolytic cleavage of cytoskeleton.Exposure of phosphatidylserine (PS) and tissue factor (TF) provide a surface for the assembly of Xase and prothrombianse to activate coagulation and induce thrombosis.Considerable levels of platelet-derived PS+ MPs are detected in healthy subjects.However,the level of PS+ and TF+ MPs,the latter of which predominantly derived from monocytes,greatly increased in thrombotic diseases,such as sepsis,cancer,and myocardial infarction.Therefore,MPs could be a useful biomarker for the evaluation of thrombosis risk.

Thyroid nodules in childhood are less prevalent but more often to be malignant than those in adulthood. The evaluation of thyroid nodules includes history collection, physical examination, laboratory examination, imaging and ifne-needle aspiration biopsy (FNAB). A history of neck irradiation, previous thyroid diseases and thyroid neoplasms in the family are high risk factors of malignant nodules. Thyroid ultrasonography is the ifrst choice of thyroid nodules assessment. FNAB is the best way to discriminate between benign and malignant lesions. Treatment option for benign thyroid nodules is follow-up. Surgery is the most effective treatment option for malignant or suspicious thyroid nodules.

Objective To investigate correlation between severity of acute pancreatitis and abnormal glucose metabolism and its prognosis.Methods A total of 158 cases of patient admitted with acute pancreatitis in the Department of Digestion of our hospital from December 2004 and December 2012 were selected,and were divided into two groups:mild acute pancreatitis (MAP; n =68) and severe acute pancreatitis (SAP; n =90) according to disease severity.The patient's sugar metabolism such as fasting plasma glucose (FPG),postprandial plasma glucose (PPG) and acute physiology and chronic health evaluation Ⅱ (APACHEII) scores of two groups were detected.Correlation analysis was carried out between glucose metabolism index and APACHEII score.The prognosis of two groups was observed.Results The FPG,PPG,fasting insulin levels (FINS),and insulin resistance index (IRI) levels of SAP group were significantly higher than that of MAP group.The insulin sensitivity index (ISI) level was lower than that of MAP (P ＜0.01).APACHE Ⅱ score of acute pancreatitis (AP) patient was positively correlated with FPG,PPG,FINS,and IRI (P ＜0.05,P ＜ 0.01),and was negatively correlated with ISI (P ＜0.05).The incidence of pseudocyst (infection,abscess,and necrosis),acute lung injury or acute respiratory distress syndrome (ARDS),and death in SAP group were significantly higher than that in MAP group (P ＜ 0.05,P＜ 0.01).Conclusions There was a close relationship between AP severity and abnormal glucose metabolism.Therefore,in treating AP,ones should be actively controlled acute pancreatitis to prevent and reduce pancreatic necrosis,decrease blood glucose to normal range,and reduce morbidity and mortality.