OBJECTIVE:To promote physicians and pharmacists obtain thorough knowledge about drug fever and its main features,clinical manifestation and identification evidence so as to improve the level of diagnosis and treatment. METHODS:The step and process of clinical pharmacists involving in medical consultation and intervention for drug fever were introduced and related knowledge of drug fever was interpreted term-by-term. RESULTS:Drug fever was common in the process of medical diagnosis and treatment. Once occurrence time of drug fever,feature of thermal variation and accompanying body reaction were observed, the relevant diagnosis can be conducted. CONCLUSION:Clinical pharmacists involving in ward around and medical consultation contribute to medical diagnosis,treatment and patient using drug safely,effectively,economically and rationally.

Breast Neoplasms ; complications ; therapy ; Female ; Humans ; Leukemia, Hairy Cell ; complications ; therapy ; Middle Aged ; Treatment Outcome

Objective To study the effects of tri-ortho-cresyl phosphate(TOCP) on mitochondrial membrane permeability in hen's nerve tissues and investigate the mechanism of the organophosphorus ester-induced delayed neurotoxicity(OPIDN).Methods Adult Roman hens were randomly divided into four groups,including three treated groups and one control group(24 in each group).The hens in the experimental groups were treated with TOCP by gavage at the single dosages of 185,375 and 750 mg/kg respectively.TOCP was dissolved in corn oil and administered at 0.65 ml/kg.The control hens received an equivalent volume of corn oil by gavage.The hens were sacrificed on the 1st,5th,15th and 21st day after treatment and the cerebrum,cerebellum,spinal cord were dissected and homogenized in ice bath.The mitochondria in these nerve tissues were extracted to determine the changes of the membrane permeability and membrane potential.Results Compared with the control group,no significant increase of the mitochondrial membrane permeability in the cerebrum was observed in treated groups.In the cerebellum,the membrane permeability in the 185 mg/kg group had no significant changes,while in the 375 and 750 mg/kg groups it increased significantly on the 1st and 5th day after TOCP treatment(P

Genetic Predisposition to Disease ; Humans ; Pneumoconiosis ; complications ; genetics ; Pulmonary Fibrosis ; etiology ; genetics

Antifungal Agents ; therapeutic use ; Child, Preschool ; Cryptococcosis ; drug therapy ; Fluconazole ; therapeutic use ; Humans ; Male

AIM: To investigate the anti - tumor effect of Weimaining (WMN) on a murine Lewis lung carcinoma (3LL) and the influence on the cell cycle. METHODS: The inhibitory rate of WMN in 3LL growth was detected by replicating the model of 3LL. The effect of the drug on 3LL cell cycle and the influence of the drug on the expression of cy clin D1 protein were investigated by flow cytometry and immunohistochemical staining. RESULTS: The results showed that the inhibitory rate of drug in 3LL is 19. 14%, 33.59%, 40. 63% and 51.56% respectively at dosage ranging from 100,cells in G0 -G1 phase and decreases the expression of cyclin D1 protein. CONCLUSION: WMN inhibits the growth of 3LL cells in vivo by decreasing the expression of cyclin D1, blocking the cells in G0 - G1 phase and preventing the cells transition from G1 to S phase while DNA is replicated.

BACKGROUND:The blood supply is essential for the repair of the meniscus after the suture of it in the injured knee joint. According to the condition of blood supply, the meniscus was divided into three zones by Arnoczk in 1982. The absolute vascularization zone, in which the distance to the combination part of meniscus and synovial membrane is inferior to 3mm, is called the red zone. The absolute devascularization zone, in which the above-mentioned distance is superior to 5 mm, is called the white zone.And the relative vascularization zone with the distance between 3 mm to 5mm is called red-white zone.OBJECTIVE: To observe and discuss the anatomical features of the blood supplyof the meniscus.DESIGN:Single sample observation.SETTING:Department of Orthopaedics, Huaxi Hospital of Sichuan University.PARTICIPANTS: The study was conducted in Huaxi Hospital of Sichuan University during the period from September 2000 to March 2001.Nineteen cases were collected, including 19 knees (38 menisci) from the subjects, 17 male and 2 female, whose age varied from 21 to 43 years old.All the samples were obtained in the amputation operation, the isolated limbs were without the change of re-transplantation and the voluntary donations from the non-minorities living in Sichuan.meniscus samples: 7 knees (14 menisci) were taken. After the femoral artery intubation and infusion of the heparinized normal saline, the vascular bed had no blood and then was infused with the normal saline and ink of 0.5 volume fraction and the infusion pressure was 13 kPa. The infused meniscus samples underwent the dehydration of gradient ethanol,the transparency of dimethylbenzene, and preservation with ilex pedunculosa, and then the blood supply of the menisci was observed and distribution of the meniscus section: 6 knees (12 menisci) with fresh and uninfused menisci were taken. The sections obtained at the sagittal plane,the coronal plane and the horizontal plane underwent hematoxylin-eosin staining. Then the tissues structure and blood supply of the meniscus samples were observed with light microscope. Another 6 knees (12meniscus) underwent the specific CD34 antibody immunohistochemical staining. Then the tissue structure and blood supply distribution of the meniscus samples were observed with the light microscope.in the devascularization zone of the meniscus.RESULTS: Nineteen cases with 38 menisci all went into the final result resourced from the medial and lateral genicular arteries and the middle genicular artery. The small vessels from them form the peripheral capillary vessel plexus and annular vessel meshwork in the synovium and joint capsule and then go into the menisci for the nutrition of the lateral The apparent lunar artery is sent by the vessel plexus around the meniscus into the body of the meniscus and arranges in the inferior,middle and superior layers and dominates 25%-30% of the zone around The vessels of the angular part of the semilunar plate cover the whole meniscus. The nutrition is supplied by the membranous synovium on the surface of the meniscus.Rich blood supply was observed in the anterior and posterior angular laminated blood supply in the body of the meniscus was newly devascularization zone of the meniscus is supplied from the joint synovial fluid is explicit.

Objective:To study the effects of Yiqi Huoxue Ruanjian Jiedu(YHRJ) drug serum on Ca 2+ and mitochondrial membrane potential (△?m) in human hepatocellular carcinoma cell line BEL-7402.Methods: YHRJ decoction was composed of Astragalus Root, White Atractylodes Rhizome, Radix Notoginseng, Fructus Polygoni Orientalis, Radix Actindiae Argutae, Flos Campsis, Pangolin Scales, Oldenlandia diffusa Roxb, and Herba Scutellariae Barbatae. Twelve New Zealand rabbits were randomly assigned into 3 groups: NS group, YHRJ equivalent dose group(YHRJD) and YHRJ high dose group(YHRJG), which were fed with NS,YHRJ equivalent dose decoction (5 times as large as normal human dose) and high dose YHRJ decoction(10 times as large as normal human dosage), respectively. Drug serum was prepared after the rabbits were fed with drugs for 5 times. BEL-7402 cells were divided into 6 groups according to different drugs, including NS group, NS+DDP group, YHRJ equivalent dose group, YHRJ equivalent dose+DDP group, YHRJ high dose group, and YHRJ high dose +DDP group. The concentrations of NS,YHRJD and YHRJG were adjusted to 100 ml/L ,and the concentration of DDP was adjusted to 4.5 mg/L. Flow cytometry was applied to detect the concentrations of Ca 2+ and △?m 8, 12, 24 and 48 h after the drugs were added.Results: The Ca 2+ concentrations at 12, 24 and 48 h after treatment were significantly higher than those of the control group(P

BACKGROUND:Perioperative treatment of emergency liver transplantation for acute hepatic failure is extremely different from common liver transplantation, due to complex conditions, high risk, several complications, and high mortality. OBJECTIVE:To summarize the experience of emergency liver transplantation for acute hepatic failure during the perioperative period, and to increase the success rate in treatment of acute hepatic failure. METHODS:A retrospective analysis was undertaken on the clinical data of 38 cases undergone emergency liver transplantation for acute hepatic failure. There were 21 male and 17 female, who aged 15-69 years. Among them, 23 cases had hepatitis B virus (including 2 cases with hepatitis B and C virus), 7 cases had Wilsons disease, 3 cases had mushroom poisoning, 2 cases had unknown liver damage, 1 case had Tripterygium wilfordi poisoning, 1 case had decompensation after partial liver resection due to trauma, and 1 case had liver transplantation from corpse. RESULTS AND CONCLUSION:The survival time of the involve patients was 13-1 740 days, and the median survival time was 634 days. Perioperative survival rate was 76%, 1-year survival rate was 63%, and 2-year survival rate was 58%. During the perioperation nine cases died of brain edema and intracranial hypertension, renal failure, severe pulmonary infection, multiple organ failure, coagulation disorders (intracranial hemorrhage, upper digestive tract hemorrhage), acute respiratory distress syndrome and primary graft non-function. At present, emergency liver transplantation is stil the most effective way for acute liver failure. Hemorrhage, infection and rejection are the leading causes of the death. Each perioperative treatment is of great significance for the success of liver transplantation and long-term survival.

BACKGROUND:Construction of vascularized bone substitutes to mimic free vascularized fibular grafting in treating large bone defects stil remains chal enges to researchers during the past years.
OBJECTIVE:To design and construct a new vascularized tissue-engineered bone graft using pre-differentiated adipose derived stem cells, fibrin sealant and porous calcium phosphate cement scaffold.
METHODS:Adipose derived stem cells isolated from rats were directly differentiated to endothelial cells and then incorporated in fibrin sealant and porous calcium phosphate cement scaffolds in vitro. Subsequently, the different composites of the three groups including vascularized tissue-engineered bone scaffold (group A), fibrin sealant and porous calcium phosphate cement scaffold (group B) and porous calcium phosphate cement scaffold (group C) were directly embedded into the quadriceps of the rats. Histological quantitative analysis and western blot assay were conducted 2 and 4 weeks after implantation.
RESULTS AND CONCLUSION:The pre-differentiated adipose derived stem cells were demonstrated in good condition after 7 days co-culturing with the fibrin sealant and porous calcium phosphate cement scaffold. The results of in vivo experiments showed that the scaffolds were in-grown together with fibrous connective tissues and blood vessels. Newly formed vessels and immature capil aries were observed in the group A. The vessel density, vessel diameter and vascular endothelial growth factor C expression in the group A were significantly higher than those in the groups B and C. Our findings demonstrated that compared with simply fibrin sealant and porous calcium phosphate cement scaffold, the combination of pre-differentiated adipose derived stem cells (endothelial differentiation) and fibrin sealant can achieve rapid angiogenesis of porous calcium phosphate cement scaffold.