In this study, we aim to investigate the protective effects of astragaloside on ultraviolet A (UVA)-induced photoaging in human fibroblasts and its possible mechanisms.

A total of 450 residents from three neighborhood committees of Ba'nan were selected to assess their willingness to accept paid influenza vaccine and the influencing factors.Among them,273(60.7％) were willing to accept paid influenza vaccine.Age,economic status,health,flu recognition and vaccine approval were the major influencing factors of vaccination.

A few of patients with lumbar disk herniation having a separation of clinical symptoms and signs and imaging features, can be found in clinic, but the traditional theory of direct mechanical compression of nerve roots by herniated nucleus pulposus can't be used to explain this abnormal protrusion of lumbar intervertebral disc. The clinical symptoms and signs of the atypical lumbar disk herniation are affected by multiple factors. The indirect mechanical compression and distraction effect of spinal nerve roots may play an important role in the occurrence of the separation, and the appearance of abnormal clinical symptoms and signs is closely related to the migration of herniated nucleus pulposus tissue, transmission of injury information in the nervous system, and the complex interactions among the nucleus pulposus, dural sac and nerve roots. Moreover,the changes of microcirculation and inflammation secondary to the herniated nucleus pulposus tissue, the hyperosteogeny in the corresponding segment of the lumbar vertebrae and the posture changes all results in a diversity of symptoms and signs in patients with lumbar intervertebral disc herniation. Besides, there exist congenital variation of lumbosacral nerve roots and vertebral bodies in some patients, and the misdiagnosis or missed diagnosis of imaging finding may occur in some cases. However, the appearance of a separation of clinical symptoms and signs and imaging examination in patients may be caused by a variety of reasons in clinic. The exact mechanism involved in the interaction among nucleus pulposus tissue, dural sac and nerve root, secondary changes of pathophysiology and biomechanics around the nucleus pulposus, the determination of lesioned responsible segments, and how to overcome the limitations of imaging all need the further researches.
Humans ; Intervertebral Disc Displacement ; complications ; diagnosis ; Lumbar Vertebrae ; Nerve Compression Syndromes ; etiology

OBJECTIVE: To discuss the influences of the concentrations of sodium cholate, cholesterol and drug, preparation temperature, ultrasonic time and power on the particle size and encapsulation efficiency of Flos Magnoliae volatile oil nano-liposome through film-ultrasonic wave technique together with membrane filter method. METHODS: The nano-liposomes were prepared by film-ultrasonic wave technique together with membrane filter method. The drug content in liposomes was determined by gas chromatography and the particle size was determined by laser light scattering granularity equipment. RESULTS: By film-ultrasonic wave technique together with membrane filter method, the liposomes in the range of 30-40 nm were prepared. The prepared nano-liposomes demonstrated good decentralization and homogeneity, and the encapsulation efficiency was (91.7+/-3)%. CONCLUSION: Film-ultrasonic wave together with membrane filter method is a simple method to fabricate nano-magnoliae liposome.

INDENA SPA Company in Italy is a multi-national company that produces and sells plant extracts. Based on its own re- search advantages in the field of Ginkgo biloba preparation, the company protects its own products market effectively through building patent portfolio around the patents of its opponent. Based on the multi-angle analysis for patent portfolio of G. biloba preparation from the aspects of application time, legal status, technical development route, and patent portfolio layout, this article provides technical reference on research and development of G. biloba preparation, and the author suggest that Chinese applicants learn techniques and layout experiences of other patents fully to enhance the level of research and patent protection level.
Chemistry, Pharmaceutical ; economics ; legislation & jurisprudence ; Ginkgo biloba ; chemistry ; Italy ; Patents as Topic ; Plant Extracts ; chemistry ; economics ; isolation & purification

Adolescent ; Adult ; Combined Modality Therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Laser Coagulation ; Male ; Middle Aged ; Phytotherapy ; Retinal Detachment ; drug therapy ; surgery

Objective To establish a rapid method for the isolation of murine peritoneal macrophages. Methods Abdominal lavage was performed with NS and the peritoneal macrophages was purified from the lavage,which was later transferred into high glucose DMEM cul-ture medium. Cell viability was measured via the typan blue staining. Purity was observed via Wright's stain. Results High purity macropha-ges with typical morphology were obtained. Conclusion A simple and realistic method was set up for the isolation of murine peritoneal mac-rophages.

Objective To explore the relationship between nuclear factor-?B(NF-?B) and(P-glycoprotein)((P-gp)) on K562/A02 cells.Methods NF-?B and P-gp of K562/A02 cells cultured with PDTC in different concentrations and time were detected by immunohisochemisty,and the relationship between them was analyzed.Results In K562/A02 cells,the expression of NF-?B and P-gp were positively correlated,and were concentration-dependent and the time dependent.Conclusion The mechanism of NF-?B regulating anti-apoptosis of K562/A02 cells is related with MDR1.

Objective To explore clinicopathological features and prognosis of juvenile granulosa cell tumor (JGCT).Methods Patients were divided into JGCT group (n=10) and the adult granulosa cell tumor (AGCT) group (n=10).The tumor samples were examined by HE and immunohistochemical methods.Results Age of JGCT group ranged from 7-31 years (average 20.5 years);90% occurred before 30 years old.Diameter of the tumors ranged from 5.5 cm to 15.0 cm,average 9.8 cm.Characteristic features included nodular arrangement,irregular formation of follicles containing muein and luteinization, atypical hyperplasia of ceils and high mitotic activity.Nuclei grooved and Call-Exner bodies were absent or rare.Survival rate was 90% in 5 years.Age of AGCT group ranged from 14-74 years (average 45.1 years);AGCT occurred mostly in over 40 years old.Atypical hyperplasia of cell,mitotic activity and luteinization were absent or rare.Nuclei grooved and eall-Exner bodies were common.Survival rate was 100% in 5 years.Immunohistochemical staining were positive for p53 at 70%,PCNA at 90% in 10 cases of JGCT and p53 at 10%,PCNA at 20% in 10 cases of AGCT(P

Objective To verify the accuracy of detecting part of biochemical markers of the new"B&G System".Methods Blood serum creatinine(SCr),urea nitrogen(BUN),serum uric acid(UA),Ca or P of the same serum example were detected by"B&G System"and Hitachi 7170S automatic biochemistry analysis instrument (7170S) respectively.Each biochemical marker was divided into two teams on the basis of measure values of 7170S, and then measure values of two methods were compared.If there were significant differences,the biochemical mark- ers's detection program of"B&G System"was corrected,and then measure values of two methods of corresponding biochemical markers were compared.Results Two methods' measure values of SCr of abnormal team,BUN of each team,SUA of each team and P of each team weren't significantly different,measure values of SCr of normal team,Ca of two teams were significantly different.After corrected"B&G System",two methods' measure values of sCr of normal team,Ca of two teams weren't significantly different.Conclusion"B&G System"through correction could accurately detect biochemical markers,and it's worth applicating in clinic.