OBJECTIVE: To determine the immunoregulatory mechanism of artesunate both in vitro and in vivo. METHODS: The immunosuppressive action of artesunate was investigated through lymphocyte proliferation and cytotoxicity was also detected in vitro. The delayed-type hypersensitivity (DTH) model in mice was used for the investigation of artesunate in vivo. The mRINA expression of T-bet, GATA-3, ROR-γt and IL-17 gene was determined by RT-PCR. ELISA assay was used to detect the contents of IL-17, and Western blot assay was applied to investigate the p38 mitogen-activated protein kinase (MAPK) activation. RESULTS: Artesunate could inhibit ConA-induced T lymphocyte and LPS-induced B lymphocyte proliferation in vitro significantly, which also showed lower toxicity than dexamethasone. Meanwhile, topical application of artesunate could both suppress the increase on ear thickness in DTH mice and inhibit the thymus index and spleen index. Furthermore, artesunate was found to regulate the expression level of transcription factor (T-bet in the GATA-3), and down-regulate ROR-γt and IL-17 expression. What's more, the production of IL-17 was decreased in ear tissue. Finally, artesunate was observed to decrease p38 mitogen-activated protein kinase (MAPK) activation. CONCLUSION: Artesunate, as a new immunomodulatory agents, might contribute to performing immunosuppression through modulating the balance of treg/Th17 and anti-inflammatory.

OBJECTIVE:To provide reference for the timely and accurate judgment of drug fever induced by Piperacillin sodi-um and tazobactam sodium for injection and rational drug use in the clinic. METHODS:The medication,clinical manifestations, laboratory examination results and the physician treatment of 30 inpatients with drug fever induced by Piperacillin sodium and tazo-bactam sodium for injection from Sept. 2013 to Sept. 2014 were analyzed retrospectively and statistically. RESULTS:Most of the drug fever induced by Piperacillin sodium and tazobactam sodium for injection occurred in continuous 7 to 14 d medication,and the cumulative dosages were between 1.3 to 2.7 g/kg;73.3% of the 30 patients had fever during the intravenous drip,with body temperature mainly≥38.5 ℃;the elevation of eosinophil and slightly increase of serum C-reactive protein (CRP) and blood cell sedimentation rate (ESR) could be used as observation indexes of drug fever,but the leucocyte reduce could’t be suitable;the body temperature dropped to normal within 24 to 48 hours after stopped using it. CONCLUSIONS:The drug fever induced by Piperacillin sodium and tazobactam sodium for injection has no correlation with the patients’gender or age,and no special diagnos-tic criteria. But it has certain correlation with duration of medication,cumulative days and dosages and it can be used as reference of judgment with the combination of hematological examination index. Clinicians should improve the understanding and attention about drug fever to stop using suspicious drug in time.

Objective To compare the efficacy and safety of intravenous infusion of recombinant human brain natriuretic peptide(rhBNP)versus Sodium Nitroprusside on treating patients with decompensated acute heart failure.Discuss rhBNP 's effect on connective tissue of heart.Methods A total of 44 patients characterized of decompensated acute heart failure were enrolled in this study.The patients were randomLy allocated to rhBNP group who receive rhBNP(1.5?g/kg bolus intravenous injection followed by 0.0075?g/(kg?min)for 24 hours,n=23)and Sodium Nitroprusside group(10 ?g/ rain for 24 hours,n=21 ).Blood pressure,heart rate,dyspnea grade,24 hours fluid in-and-output and improvement in patient symptoms and signs were evaluated and adverse events were documented.Results Dyspnea and symptom im- provements were much more significant in rhBNP group compared to Sodium Nitroprusside group(P

? Intraoperative floppy iris syndrome is a particular symptom in phacoemulsification, and it is often occurred in patients with the history of using alpha-1 adrenergic antagonist before cataract surgery. Although the pupil be fully mydriatic before the operation, the progressive constricting of the pupil, the relaxing of iris even the iridoptosis and the unstable anterior chamber still can be found during the phacoemulsification. If treated inappropriately, severe complications and terrible outcomes may happen, which would influenced the surgery effectives. This paper reviewed the epidemiological characteristics, pathogenesis, risk factors, clinical manifestations, complications, and coping strategies of the intraoperative floppy iris syndrome.

OBJECTIVE: To observe the hypoglycemic effect of raspberry ketone and its mechanism on diabetic model mice induced by alloxan. METHODS: Healthy male KM mice were used to establish diabetic models by injecting alloxan via tail vein, and then randomly divided into model control group, metformin group (90 mg · kg-1), and raspberry ketone low, middle and high (200, 400, 800 mg · kg-1) dose groups. Normal control group was set. All groups had been treated for 20 d. Fasting blood glucose (FBG) was measured on 0, 7 and 14 d. After 20 d, glucose tolerance test was carried out. Fast insulin (FINS) of the mice were measured, and the insulin sensitivity index(ISI) was calculated by determining the contents of FBG and FINS. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malonaldehyde (MDA) in serum were measured. Pathological changes of pancreas and insulin expression were examined. RESULTS: Raspberry ketone could effectively control the increase of fasting plasma glucose (P < 0.05) and reduce the role of the area under the glucose curve. Compared with the model group, the levels of FINS, SOD and GSH-Px activity were significantly improved (P < 0.05), and MDA content was decreased (P < 0.05) in the high dose raspberry ketone group. The pathological symptoms of pancreas were relieved in the high dose group. CONCLUSION: Raspberry ketone can effectively control blood glucose, protect pancreatic islet β cells, and improve insulin secretion in diabetic mice by effectively inhibiting the oxidative stress.

Acute Disease ; Animals ; Dimethoate ; analogs & derivatives ; poisoning ; Disease Models, Animal ; Male ; Mice ; Muscle Weakness ; chemically induced ; pathology ; Muscles ; pathology

20 children aged 3 years ±3 months with dental caries were randomly divided into 2 groups(n =1 0).The caries tissue was re-moved by conventional bur and Er:YAG laser respectively without the use of mouth speculum,mouth gag and binding belts.In laser group the success rate of treatment at first visit and return visit was higher(P <0.05)and children's dental anxiety grad was lower(P <0.05).The Er:YAG laser is more effective in easing children's dental anxiety and in the dental treatment.

0 05),but the difference was significant after administration of Cisapride two weeks and four weeks later(7 00?3 12,4 20?2 39 vs 4 90?2 45,2 10?1 19,P0 05),but the difference was statistically significant with Cisapride administrated(r=0 47,P

Purpose:To obtain the optimum irradiation technique in different plans of conventional radiotherapy(2DRT)and conformal radiotherapy(3DCRT)and between different conformal radiotherapy techniques for esophageal carcinoma as compared by 3D-TPS.Methods:10 patients with esophageal carcinoma were enrolled in this study.The conventional treatment plan and four conformal technique plans were created for each patient.Plan 1 was the same as the 2DRT,plan 2 consisted of anterior and two posterior oblique fields,plan 3 consisted of posterior and two anterior oblique fields,plan 4 consisted of 2 dynamic arc fields.Different plans were compared using DVH.Results:①On average,PTV of 2DRT was the same as that of 3DCRT in DVH.The difference in maximum dose to spinal cord was obvious.The left lung dose in D_(mean)?D_(min)?V_(30) in 2DRT was significantly increased as compared to 3DCRT(P

Objective: To break the seed dormancy and improve the germination rate of the Nirtaria sibirica seeds. Methods: N. sibirica seeds were treated with soaking in warm water, storing with moisture sand, and immersing seed in H2SO4 (98%) and gibberellic acid (GA), then the germination rate, germination vigor, and germination peak period of the seeds were determined. Results: The germination rate and germination vigor were 30% and 10% under soaking in warm water. The seeds began to germinate on the day 7 and reached the germination peak on the day 10.The germination rate and germination vigor were 55.5% and 26.5% under storing with moisture sand for 37 d. The seeds began to germinate on the day 2 and reached the germination peak on the day 10.The germination rate and germination vigor were 86.8% and 60.3% under storing with moisture sand for 95 d. The seeds began to germinate on the day 2 and reached the germination peak on the day 7.The germination rate and germination vigor were both 90.0% under being treated with 98% H2SO4 for 2 h. The seeds began to germinate on the day 1 and reached the germination peak on the day 4.Seeds after being treated with 98% H2SO4 for 2 h were sowed on the dark medium of MS + 0.5 g/mL BA + 0.5 g/mL GA, and the germination rate and germination vigor both reached to 98.1%. The seeds began to germinate on the day 1 and reached the germination peak on the day 4. Conclusion: The dormancy of N. sibirica seeds is caused by hard seed vessels. The best way of breaking the seed dormancy is first treated with 98% H2SO4 for 2 h and then cultured in the dark medium of MS + 0.5 g/mL BA + 0.5 g/mL GA, which could effectively break the dormancy of hard seeds and reach a high germination rate.