Head ; surgery ; Humans ; Neck ; surgery ; Reconstructive Surgical Procedures

AIM:To investigate the effects of ursolic acid ( UA) on the migration and invasion of human lung cancer cell line A549, and to explore its mechanism .METHODS:The cell viability was detected by MTT assay .The ex-pression of miRNA-133a was detected in the A549 cells treated with UA by real-time PCR.The miRNA-133a mimics and inhibitor were transfected into the A 549 cells, and the transfection efficiency was analyzed by real-time PCR.The cell mi-gratory and invasive abilities were determined by wound healing and Transwell methods , respectively .RESULTS:The via-bility of the human lung cancer A549 cells was significantly inhibited by UA in a dose-dependent manner (P<0.05).IC50 of UA (24 h) for lung cancer A549 cells was 31.04 μmol/L.UA treatment significantly inhibited the migratory and inva-sive abilities of A549 cells in a concentration-dependent manner , accompanied by significantly elevation of miRNA-133a expression.The mimics and inhibitor of miRNA-133a significantly upregulated and downregulated the expression of miRNA -133a in the transfected A549 cells, respectively.In addition, the viability of the A549 cells was decreased extremely after tansfected with the miRNA-133a mimics (P<0.01), so did the results of the cell migration and invasion test .The A549 cells tansfected with the miRNA-133a inhibitor showed an opposite changes of the cell viability , migration and invasion . CONCLUSION:UA inhibited the viability , migration and invasion of lung cancer A 549 cells by elevating the expression of miRNA-133a.

0.05).CONCLUSION A similar MMN exists in guinea pigs and human.MMN has a process of development,differentiation and maturation with aging. Guinea pigs can serve as the candidate animal for MMN research.Comparing with ABR,MMN is more sensitive to evaluate the central auditory function.

Objectives To evaluate the effects of“the Seven Acupoints of the Cranial Base”(SACB) in improving quality of life(QOL)of patients with PD. Methods The study Subjects were seventy(70)patients with a diagnosis of Parkinson's disease. The Subjects were divided into two groups, i.e. acupuncture group and drug group.The former was treated by SACB every two days,while the latter took Levodopa and Benserazide Tablet250mg tid. The subjects all received treatment for 6 months. PDQ-39,UPDRSⅡand PDSS were adopted to measure quality of life at 4 time points. Results The total effective rate of the treatment group was 85.71%, the control group was 71.43%. And there was a statistical difference of level distribution between the two groups(P<0.01). The total score of PDQ-39, UPDRSⅡ and PDSS of patients in the treatment group [(20.41± 11.64), (9.09±5.02), (126.31±8.37)]were significantly improved compared with the control group[(27.48± 8.69), (10.29±4.93), (109.94±8.11), P<0.01]. The 5 dimension score of PDQ-39, and the 4 single item score of UPDRSⅡ in the treatment group[(8.60±5.07), (3.20±2.08), (2.34±1.73), (1.20±1.68), (1.63±1.96),(1.31±0.68), (0.77±0.94), (0.91±0.74), (0.77±0.65)]were significantly improved compared with the control group[(11.69±4.11), (5.09±3.72), (5.37±1.99), (2.86±2.33), (2.74±2.08), (1.74±0.98), (1.31±0.93), (1.43±0.78), (1.20±0.68), P<0.01 or 0.05]. Conclusion SACB can markedly and multi-dimensionally improve the QOL of patients with PD and has a higher efficacy than Levodopa and Benserazide Tablet.

Objective:To discuss the application of tracer methodology in the management of medical high-value consumables, find out the problems and existing risks, and propose possible solutions. Methods: The system tracing based on high-value consumables and the case tracing based on patient were used to track and check the medical high-value consumables in hospital. The inspectors summarized and discussed the issues as well as the possible risks after inspection, to develop the appropriate improvements.Results: There were many problems that were found in the management of medical high-value consumables in this tracing program, of which the main aspects were the species introduction, the implementation of procurement and relative departments, and the organization and leadership among departments.Conclusion:The management of medical high-value consumables is an important part of hospital management, and the tracer methodology provides an effective guarantee for the medical equipment management in hospital.

Ion channels are membrane proteins that are found in a number of viruses and which are of crucial physiological importance in the viral life cycle.They have one common feature in that their action mode involves a change of electrochemical or proton gradient across the bilayer lipid membrane which modulates viral or cellular activity.We will discuss a group of viral channel proteins that belong to the viroproin family,and which participate in a number of viral functions including promoting the release of viral particles from cells.Blocking these channel-forming proteins may be"lethal",which can be a suitable and potential therapeutic strategy.In this review we discuss seven ion channels of viruses which can lead serious infections in human beings: M2 of influenza A,NB and BM2 of influenza B,CM2 of influenza C,Vpu of HIV-1,p7 of HCV and 2B of picornaviruses.

Objective To investigate the effect of preemptive cytomegalovirus infection(CMV) on acute rejection (AR) and assess the value of preemptive prophylactic antivirus therapy. Methods Retrospective study of 116 transplant recipients from September 2001 to February 2005 was made. The CMV infection was diagnosed by CMV-PP65 antigen tests. According to the result of CMV-PP65 antigen, 116 transplant recipients were divided into preemptive CMV infected group and non-infected group. The preemptive CMV infected recipients were subdivided into valacyclovir-treated group and non-valacyclovir-treated group. The impact of preemptive cytomegalovirus infection on acute rejection was analyzed. At the same time, CMV-PP65 antigen in 35 healthy subjects was detected. Results Seventy-four recipients had preemptive CMV infection, and the infection rate of renal transplant recipients was higher than healthy subjects (63.8% vs 14.3%, P

Objective To understand drug resistance in patients with human immunodeficiency virus infection and acquired immunodeficiency syndrome (HIV/AIDS)after antiretroviral therapy(ART).Methods Articles about drug resistance in HIV/AIDS patients after ART were retrieved from Wangfang data,China National Knowledge Infrastructure(CNKI),and PubMed. Meta analysis were performed.Results 15 articles (1 English and 14 Chinese) were extracted,quality score of 3,4,and 8 articles was 6,7,and 8 respectively. Heterogeneity of the included stud-ies showed the difference was significant (Q= 45.98,P<0.001),there was heterogeneity,random-effects models was conducted for Meta analysis,which revealed that the overall drug resistance rate in HIV/AIDS patients in China was 4% (4% -5% );Begg's test showed that P= 0.012,there was publication bias. After articles were excluded through sensitivity analysis,the combined effect was still 4% (4% -5% ).Conclusion The overall drug resistance rate in Chinese HIV/AIDS patients after ART is not high.

Objective To evaluate the value of MSCT three-dimensional reconstruction postprocessing technique for operation of Scoliosis Orthomorphia. Methods 18 cases of scoliosis were performed with MSCT to be reconstructed by MPR, VRT. The three-dimensional reconstruction images displayed traverse, coronal and sagittal plane and measures data. Results By measuring data of 18 cases by means of the methods above, all parameters were accurate. Conclusion The three-dimensional reconstruction of MSCT makes up the disadvantages of X-ray. Any retrospective reconstruction can be done to the collected data. So patients can avoid being projected repeatedly and radiation can be reduced. Various parameters of the vertebrae concerning spine, spinal canal and the pedicle plane can be offered, providing reliable reference for pre-operations.

Objective To measure the volume of liver, the volume of mass of liver and the ratio of mass/righe lobe with the Volume Evaluation of MSCT, as a reference of liver function after resection of liver mass. Methods All cases were scanned and reconstructed with MSCT,and the volume of right liver, the volume of mass of right liver and its ratio were obtained with Volume Evaluation of MSCT. Results The method of measuring volume of liver with Volume Evaluation is simple and accurate. Conclusion It can be a important reference before and after resection of liver mass.