Objective To investigate the effects of different concentrations of sevoflurane on adhesion and expression of CD 44 in human breast cancer cell line MCF‐7 .Methods The cells were randomly divided into 4 groups :control group and 3 sevoflurane groups exposed to 1 .7% ,3 .4% and 5 .1% sevollurane for 2 ,4 and 6 h respectively .Cell adhesion rate was detected by adhesion test and the expression of CD44 mRNA and protein was determined by qRT‐PCR .Results in Treat 1 ,2 ,3 ,the adhesion rate at 2 ,4 ,6 h were lower than that before treat ,mRNA expression of CD44 reduced (P<0 .05);the adhesion rate of Treat 2 and Treat 3 at 4 and 6 h were lower than that of 2 h ,and the mRNA expression of CD44 reduced (P<0 .05);the adhesion rate of Treat 2 and Treat 3 at 6 h were lower than that of 4 h ,and the mRNA expression of CD44 reduced (P<0 .05);compared with Control group ,the adhesion rate of Treat 1 ,2 and 3 at 2 ,4 ,6 h were all lower ,mRNA expression of CD44 reduced (P<0 .05);the differences between each group were statisyicaly significant(P<0 .05) .Conclusion Sevoflurane can inhibit cell adhesion in a concentration and duration of exposure dependent manner ,and its mechanism could be related with the down regulation of CD44 expression .

Arterio-Arterial Fistula ; complications ; Coronary Aneurysm ; complications ; Heart Ventricles ; Humans ; Male ; Middle Aged

Objective To study the effect and mechanism of the Fosinopril combined with Fenofibrate on the prevent?ing of diabetic retinopathy. Methods A total of 150 viripotent ICR mice(100 male mice, 50 female mice) were randomly di?vided into five groups(n=30), including A group (Sham group), B group (Model group), C group [Fosinopril prevented group, 20 mg/(kg·d)], D group [Fenofibrate prevented group, 400 mg/(kg·d)] and E group (Fosinopril combined with Fenofibrate pre?vented group). The expression levels of Bax and Bcl-2 gene mRNA were determined by RT-PCR method. TUNEL staining method was used to detect the apoptosisi of retinal cells. Results The Bcl-2 mRNA of A group, Bax mRNA of B group were higher than those of other four groups. Bcl-2 mRNA of E group was higher than that of C group and D group, while the Bax mRNA was lower than those of two groups(all P<0.05). The TUNEL index of B group was the highest than other groups, which of E group

BACKGROUND: The formation of long-term memory includes acquisition, consolidation and reconsolidation. Reconsolidation is very important for the new memory transforming into stable memory. Stress is an important environmental factor in the process of learning and memory. Corticosterone is very important for stress response. At present, research about the impact of corticosterone on reconsolidation of novel object recognition memory is less. Thus, it is very important to test the impact of corticosterone on reconsolidation of novel object recognition memory in rats.
OBJECTIVE: To analyze the effects of corticosterone on the reconsolidation of novel object recognition memory in rats.
METHODS: (1) The rats were intraperitoneal y injected with corticosterone (0.1, 1 and 3 mg/kg) immediately after reactivation. The discrimination index was used to assess memory performance and calculated as the difference in time exploring the novel and familiar objects. (2) The rats were intraperitoneal y injected with corticosterone (3 mg/kg) 6 hours after reactivation. The discrimination index was used to assess memory performance and calculated as the difference in time exploring the novel and familiar objects. (3) The rats were intraperitoneal y injected with corticosterone 3 mg/kg without reactivation. The discrimination index was used to assess memory performance and calculated as the difference in time exploring the novel and familiar objects.
RESULTS AND CONCLUSION: Immediate injection of corticosterone 3 mg/kg after reactivation significantly decreased the discrimination index. At 6 hours after reactivation or without reactivation, corticosterone administration did not impact the discrimination index. These results confirmed that corticosterone administration immediately after reactivation impairs the reconsolidation of novel object recognition memory. The damage effect depends on the reactivation experience and the specific time window after reactivation.

Objective To explore the correlation between self-concept and subjective quality of life among children with tic disorders (TD). Methods The Piers-Harris Children's Self-concept Scale (PHCSS) and the Inventory of Subjective Life Quality (ISLQ) were complet-ed by 65 children with TD and 71 normal children from March to September, 2015; and analyzed with single factor analysis, correlation analysis and multi factors regression analysis. Results There was no significant difference in PHCSS scores between the TD children with and normal ones (t<1.624, P>0.05). The ISLQ scores of school life, self-awareness and cognitive component were less in the TD children than in the normal ones (t>2.051, P<0.05). The PHCSS subscores correlated with ISLQ subscores (P<0.05), except the following:PHCSS scores of behavior did not correlate with ISLQ scores of peer relationship, school life, living environment, self-awareness and cognitive com-ponent;PHCSS scores of intelligence and school environment did not correlate with ISLQ scores of family life, peer interaction and the liv-ing environment;PHCSS scores of physical appearance and properties did not correlate with ISLQ scores of family life and living environ-ment;PHCSS scores of anxiety did not correlate with ISLQ scores of family life, peer interaction and cognitive component;PHCSS scores of affiliation did not correlate with ISLQ scores of peer interaction;and PHCSS total score did not correlate with ISLQ scores of family life and living environment. PHCSS scores of affiliation were the factor related with the quality of life (β=0.301, P<0.05). Conclusion The quali-ty of life may be impaired in the children with TD, which may associate with the self-concept and need further intervention.

Heparinases,a kind of polysaccharide lyases,can degrade heparin and heparin sulfate to low molecular weight polysaccharides.It has been noted that many bacteria have heparinases although only few of them have been purified and characterized.Heparinases I,II and III from Flavobacterium heparinum have been extensively studied for many years and been commercialized recently.Heparinases have some important applications in the industry and clinic as well as in the determination of heparin structure,which is a very important anticoagulant drug used world-widely.The recent progresses in isolation of heparinase-producing bacteria,genome mapping of heparinase homologs in sequenced bacteria and archaea genomes,purification of heparinases and the study of their biochemistry and regulation were reviewed.The recombinant expression of these enzymes as well as important applications of heparinases and their potential applications in the future will also be highlighted.

AIM To investigate the influence on change of SOD and MDA in the blood of rats which had cellular immunoreactive colitis by Fengliao Changweikang Granule (CWKG). METHODS Male Wistar rats were divided into six groups. Numbers of each group were 32. Animal model of rat cellular iumynoreactive colitis was made by TNBS and ethanol mixed liguid enema. Rats were killed at the 1st, 3rd, 7th and 21st day respectively. Gross morphologic observation of intestinal wall was made, SOD vitality and MDA content in blood were determined. RESULTS At day 7 and 21, the intestinal wall damage criterion value of large dosage group was lower than model group ( P

Objective To investigate the characteristic of intelligence of children with attention deficit hyperactivity disorder (ADHD).Methods 60 ADHD children were estimated with Wechsler Intelligence Scale for Children-fourth edition (WISC-Ⅳ). Results The full scale intelligence quotient (FSIQ) and 4 indices of ADHD children were lower than the average (P<0.05). All the subtests scores of ADHD children were lower than the average (P<0.05) except decoding. Conclusion The general intelligence and abilities of verbal comprehension, reasoning,working memory and operation speed are in the normal range in ADHD children, but poorer than normal children.

Objective To screen the mutations of MYOC gene in a Chinese primary open angle glaucoma (POAG) family from Cbengqing and investigate the relationship between the mutations in MYOC/TIGR gene and POAG.Methods In a large 4-generation glaucoma family, myocilin gene (MYOC) was screened in 39 family members, 8 of which were confirmed patients. Normal controls included 100 normal Chinese subjects.The known mutations of MYOC gene ( including G34C, C136T, G144T, G227A, C624G,G736A, C1009G, A1036G, C1081T, G1099A, G1138A, A1139C, T1430A, C1441A and C1442T) were detected by single strand conformation polymorphism(SSCP) , po]ymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis and DNA sequencing.Results G227A mutation was detected in 2 POAG patients and 1 asymptomatic patient, but not in the controls.Cl009del mutation was identified in all patients of the pedigree and an offspring member but not in the controls. No other mutations were detected.Since the C1009del mutation was revealed for the first time, a new GenBank number FJ237047 correponding to ACI62293 was applied.Conclusions The G227A mutation is a known site and there is no relationship between G227A mutation and glaucoma. But C1009del may be related to glaucoma which suggests that morbidity could be higher in the relatives of POAG than the controls.

Aim To investigate whether necroptosis mediates chemical hypoxia-induced HT22 mouse hippocampal cell injury and inflammation.Methods HT22 hippocampal cells were exposed to cobalt chloride (CoCl2) to establish a model of the chemical hypoxia-induced injury and inflammation.The expression level of RIP3 (an index of necroptosis) was determined by Western blot.Cell counter kit-8 (CCK-8) assay was used to test the cell viability.Lactate dehydrogenase (LDH) activity in the culture medium was measured with commercial kits.Mitochondrial membrane potential (MMP) was examined by rhodamine123 staining followed by photofluorography.The intracellular level of reactive oxygen species (ROS) was detected by 2', 7'-dichlorfluorescein-diacetate (DCFH-DA) staining followed by photofluorography.The secretion levels of interleukin-1β (IL-1β) and tumor necrosis factor-a (TNF-α) were measured by ELISA.Results Treatment of HT22 hippocampal cells with 600 μmol·L-1 CoCl2 for 36 h markedly induced cytotoxicity, leading to a decrease in cell viability to (52.0±2.65) % , indicating that chemical hypoxia-induced cellular injury model was successfully set up.Besides, CoCl2 induced considerable injuries and inflammation, evidenced by increases in LDH activity, ROS production, MMP loss, as well as the secretion levels of IL-1β and TNF-α.Co-treatment of the cells with 40~100 μmol·L-1 Nec-1 (a specific inhibitor of necroptosis) and CoCl2 markedly attenuated the decrease in viability induced by CoCl2, reaching the best anti-cytotoxicity inhibitory effect at 80 μmol·L-1.Meanwhile, the co-treatment with 80 μmol·L-1 Nec-1 blocked the above injuries and inflammatory response induced by CoCl2.In addition, treatment of HT22 hippocampal cells for 6~48 h up-regulated the expression of RIP3, and Nec-1 alleviated the up-regulation of RIP3 expression level induced by CoCl2.Conclusion Necroptosis mediates chemical hypoxia-induced HT22 hippocampal cell injury and inflammation.