BACKGROUND: The denervated skeletal muscle is chiefly characteristic as the loss of neurotrophic factor, which may cause the muscle atrophy degeneration and fibrosis. OBJECTIVE: To investigate the effect on the muscle satellite cells and muscle atrophy by the implantation of basic fibroblast growth factor (bFGF) released through silicon tube in the gastrocnemius. DESIGN, TIME AND SETTING: Randomly control animal experiment was finished at the Animal Experiment Laboratory of Shenyang Medical College Fengtian Hospital in November 2006. MATERIALS: Twenty-eight Wistar healthy male rats (weighing 250-300 g) were adopted. Silicon tube was prepared by the encapsulation with bFGF or physiological saline, and sealed with 502 glue. METHODS: The sciatic nerve of left lower limb was cut off, and then gastrocnemius was taken out. All of 28 Wistar rats were divided into experiment group and control group randomly, each group involved 14 rats. The experimental gastrocnemius was encapsulated with silicon tube containing bFGF, while the control group with physiological saline. MAIN OUTCOME MEASURES: At day 30 postoperatively, the following indicators were evaluated: wet weight of gastrocnemius and wave amplitude of muscle fibrillation potential; proliferating cell nuclear antigen positive nuclei on surface of muscle fibers, diameter and section area of gastrocnemius fiber under light microscope; ultrastructure of gastrocnemius under electron microscope. RESULTS: The number of muscle satellite cell nuclei in the experimental group was more than that in the control group (P

Objective To evaluate the protective effect of prior ?-adrenergic blocker therapy to patients who have PCI therapy.Methods We analyzed 210 consecutive patients undergoing ?-adrenergic blocker,of whom 105 did ?-adrenergic blocker therapy,105 had not.CK-MB、E、NE were tested before PCI,and 6~8 h,16~24 h after PCI.Procedural complications in hospital and 1-year outside outcomes were evaluated.Results (1)There was no significant difference between the two groups on CK-MB、E、NE.(2)Both the in-hospital(2.1% vs 4.1%)and one year (5.5% vs 7.1%)mortality was lower in ?-group than in no-? group.Conclusion Prior ?-adrenergic blocker therapy can reduce the incidence of cardic events.

Adenoviruses, Human ; Child ; Humans ; Orthomyxoviridae ; Respiratory Syncytial Viruses ; Respiratory Tract Infections ; virology

Objective To develop a stable and sensitive microemulsion liquid chromatography method by using oil-in-water microemulsion mobile phase;To simultaneously determine five major active lignans, namely, schizandrin, schisandrol B, schisantherin A, deoxyschizandrin, and schisandrin B, in Schisandrae Sphenantherae Fructus and Schisandrae Chinensis Fructus. Methods The quantitative determination was conducted by using Kromasil C18 column (250 mm × 4.6 mm, 5μm). The influence of the composition of the microemulsion system on separation effect and retention time was studied. The method was optimized to sodium dodecyl sulfonate 3.3%(SDS, W/V), n-octanol 1.2% (V/V) and n-butanol 6.6% (V/V) in 20 mmol/L phosphate buffer (adjusted to pH 7.0 with orthophosphoric acid and sodium hydroxide). The flow rate was set at 0.7 mL/min. The compound peaks were detected by UV at 254 nm. The column temperature was 25 ℃. Results Under the optimized conditions, baseline separation of 5 lignans was achieved in 20 min, and the separation was satisfying. Schizandrin, schisandrol B, schisantherin A, deoxyschizandrin, and schisandrin B were in linear relation within the ranges of 2.0-155.0, 8.3-166.7, 8.8-175.8, 8.3-166.7, 8.9-177.1μg/mL (r>0.999). The limit of detection ranged from 0.06μg/mL to 0.18μg/mL, and the limit of quantitation ranged from 0.17μg/mL to 0.55μg/mL. The average recovery values were between 97.22%and 100.8%. Conclusion This method is simple, accurate and reliable, and can be successfully applied to simultaneous determination of five major active lignans in 13 batches of Schisandrae Sphenantherae Fructus and Schisandrae Chinensis Fructus from different producing areas in China.

Animals ; Diet ; Enterocolitis, Necrotizing ; etiology ; metabolism ; Humans ; Infant, Newborn ; NF-kappa B ; physiology ; Platelet Activating Factor ; chemistry ; metabolism ; Risk Factors ; Tumor Necrosis Factor-alpha ; physiology

Objective To explore diagnosis and prognosis of heart failure in children by measuring brain natriurettic peptide(BNP) and cardiac troponin I(CTnI).Methods Forty-one children subjects with heart failure were chosen,and then they were divided into three stages,early stage of heart failure,stage of heart failure and stage of recovery according to the ROSS cardiac function score method.In contrast with the former group,41 healthy children were chosen as control group.BNP and CTnI levels of blood plasma were analyzed by radioimmunoassay(RIA) and chemiluminescent immunoassay(CLIA) respectively,the relationship between the BNP level of blood plasma and prognosis of CHF group was analyzed.Results At early stage of heart failure,BNP and CTnI levels began to rise.They amount to highest peaks at stage of heart failure and went down at stage of recovery.But they were still higher than those of control group(P

0.05).No partial side-effect of the medicine were discorvered in the observation process.Conclusion Long-term inspiration of low-dose GCS to treating the babies and infants with asthma has reliable security.

Objective: To explore the relationship between continuous cropping obstacle and autotoxicity of Angelica sinensis, autotoxic effect and organic compounds of rhizosphere soil water extract were determined. Methods: Distilled water (CK), water extract of rhizosphere soil (100, 125, 250, and 500 mg/ mL) were applied to testing their effect on early development of A. sinensis. Seed germination rate, germination index, elongation of radicle and embryo were recorded, and GC-MS was conducted for the compound identification in the extract. Results: The water extract at concentraion as low as 125 mg/mL significantly inhibited the germination and seedling growth of A. sinensis, and this inhibitory effect generally increased with the increase of the concentration of water extracts. Seventeen compounds in rhizosphere soil water extract were identified, including organic acids, ketones, aldehydes, esters, and hydrocarbons, most of them are allelophathic substance. Conclusion: Water extracts from A. sinensis rhizosphere soil have inhibitory effects on A. sinensis germination and seedling growth, and this inhibitory effect generally increased with the increases of the water extract concentration at a certain ranges. In conclusion, there is autotoxicity in continuous cropping of A. sinensis, which is one of the causes of problems associated with the continuous cropping obstacle of a single plant species.

With the existence of capsules,lens regeneration occurs in some mammals after extracapsular lens extraction.It is usually thought that lens regenerates from resident lens epithelial cells (LECs) in the capsule.However,lens regeneration dose not mean simple redupilication of development,and transparency of the lens is affected by irregular growth,migration and transdifferention of the resident LECs.Previous studies mainly focus on the dysplasia of LECs,but update theory about lens regeneration is proposed to be associated with stem cells.Some views and suggestions in lens regeneration are concerned in current researches to better illuminate the mechanism and therapy of posterior capsular opacity.