Objective To investigate the low back pain(LBP)of clinic nurses and find out the understanding of related knowledge of LBP in nurses,and improve the occupational protection of nurses.Methods Investigation was carried out in 692 nurses by self-designed questionnaires.Results The incidence of LBP in nurses was 97.4%.The knowledge of LBP and the related occupational protection in nurses were greatly deficient.Conclusions The hospital should pay attention to the protection of LBP in nurses and improve their health.

Objective To investigate the leptin in continuous ambulatory peritoneal dialysis(CAPD)and its influen- cing factors.Methods 30 CAPD patients and 30 henhhy controls were enrolled in the study.Leptin was measured by ELISA.C reactive protein(CRP)by transmisson turbidmetry.Body mass index(BMI),fasting insulin(FINS), blood urea nitrogen(BUN),serum creatinine(Scr)etc were also determined.These patients were classified to two nutritional categories based on SGA normal nutrition and malnutrition.The relationship between serum leptin concentra- tion and these indices was analysed.Results Serum leptin levels in CAPD group was significantly higher than those of healthy control(P

OBJECTIVETo observe the clinical efficacy of Jiakangling Capsule (JC) combined with reduction of 1311 in treatment of Graves hyperthyroidism.

METHODSTotally 387 Graves hyperthyroidism patients were randomly assigned to the treatment group (200 cases) and the control group (187 cases). Patients in the treatment group took JC combined with reduction of 131I. The 131I dosage per gram of thyroid tissue was 50-80 microCi. They additionally took JC one week after taking 1311 for one consecutive month. Patients in the control group took 131 routinely as one disposable treatment. The 131I dosage per gram of thyroid tissue was 70-120 microCi, without using JC or other anti-thyroid drugs. All patients were reexamined after 24-month treatment. Whether hyperthyroidism was cured, incurred, or permanent was observed. Efficacies of thyroglobulin antibody (TGAb) and thyroid microsome antibody (TMAb) were compared between the two groups.

RESULTSCompared with the control group, the incurred ratio increased in the treatment group [3.2% (6/187) vs. 16.0% (32/200), P < 0.01], the incurred ratio of strong positive TGAb and TMAb patients increased [3.5% (2/57) vs. 27.1% (16/59), P < 0.01], the permanent hypothyroidism ratio decreased [21.1% (12/57) vs. 3.4% (2/59), P < 0.05 ].

CONCLUSIONJC combined with reduction of 1311 was superior in treating Graves hyperthyroidism induced permanent hypothyroidism than routine 1311 treatment, especially for strong positive TGAb and TMAb patients.

Autoantibodies ; Capsules ; Drugs, Chinese Herbal ; therapeutic use ; Graves Disease ; drug therapy ; Humans ; Hyperthyroidism ; drug therapy ; Hypothyroidism ; Iodine Radioisotopes

Objective To establish the quality standard for Guishao Zhenxian Tablets. Methods Radix Glycyrrhizae,Radix Codonopsis,Radix Bupleuri were identified by TLC. The content of paeoniflorin in the preparation was determined by HPLC. Results The spots of TLC color spectrum were distinct and there was nointerference from the negative control. The calibration curve was linear over the concentration range of 0.245 2～0.735 6 ?g for paeoniflorin (r=0.999 7) and the average recovery was 98.3%,RSD=1.52% (n=5). Conclusion The established quality standard is convenient and reliable,and can be used to control the quality of Guishao Zhenxian Tablets more effectively.

Objective To investigate the common causes of acute renal failure(ARF) in the elderly hospitalized patients and the effect of continuous peritoneal dialysis(PD) by bedside intubation.Methods The data of hospitalized patients with ARF including 28 elderly patients (mean age 68.2?9.6 yrs) and 13 adult patients (mean age 39.4?9.1 yrs) were studied.Of them,32 patients received PD by bedside intubation.The nosogenesis of ARF was analyzed and the effect of emergency PD by bedside cmpared between two groups.Results Among various causes,prerenal factors accounted for 32.1%,infection and wound of bad control 39.3%,unreasonable use of nephrotoxic drugs 14.3%,which totally accounted for 85.7%.Of 21 elderly patients (76%) receiving PD treatment,11 (52.4%) acquired completely remission,5 (23.8%) turned to chronic PD,the successful rate of intensive care being 81.0%,and the death rate being 19.1%.Conclusion Prerenal factors,infection,wound of bad and unreasonable use of nephrotoxic drugs are the common causes of ARF in the elderly hospitalized patients.PD by bedside intubation is safe,simple and effective.

Objective To compare the safety and recent efficacy between transcatheter and surgical closure of secondum atrial septal defect over 50 years of age. Methods Retrospective analysis was done on 66 patients over 50 years of age who underwent transcatheter (30 cases) and surgical (36 cases) closure of secondum atrial septal defect.The median age was 60.8?7.1 years old in the transcatheter closure group and (57.5?6.3) years old in the surgical closure group( P=0.04).The median sizes of atrial septal defect in the transcatheter and the surgical closure group were 24.9?7.2 mm and 30.4?11.0 mm respectively (P=0.02). We compared the recent efficacy、 complications and prognosis between two groups. Results The instant procedural success rate was 93.3% for the transcatheter closure group and 100% for the surgical group (P=0.85). 2 procedures in the transcatheter closure group failed, 1 patient (2.6%) in the surgical group died during hospitalization and 9 patients (25.0%) underwent Devage tricuspid annuloplasty. Newly onset atrial arrhythmias occurred in 3.3% in the transcatheter closure group and 22.2% in the surgical closure group (P=0.03). After the procedures, right atrial and ventricular dimensions were decreased from 38.0?9.0 mm to 35.2?10.1 mm and from 44.2?8.6 mm to 36.2?9.0 mm in the transcatheter closure group whereas in the surgial group, the atrial and ventricular sizes fell from 44.1?8.2 mm to 38.2?9.5 mm and 49.0?10.2 mm to 37.1?11.0 mm respectively (P

Objective To analyze the pathological diagnosis of patients with clinically diagnosed eczema,to describe the spectrum of skin diseases tending to be misdiagnosed as eczema,and to investigate factors associated with their misdiagnosis.Methods A retrospective study was performed on 400 patients who were clinically diagnosed as eczema and received pathological examination at the dermatology clinic of Peking University Third Hospital from August 2006 to April 2013.Skin biopsy specimens were re-reviewed for these patients,and pathological diagnosis was made in combination with clinical presentations.Results Of the 400 outpatients with clinically diagnosed eczema,110 (27.5％) were finally diagnosed as non-eczema skin diseases pathologically,including 16 cases of psoriasis,13 bullous pemphigoid (BP),11 lichen planus (LP),9 cutaneous amyloidosis,8 mycosis fungoides (MF),14 skin malignancies,and 39 other skin diseases.The highest misdiagnosis rate was observed in people aged 60 to 79 years (33.9％) and lesions at the genital sites (46.2％).Conclusions Many conditions tend to be misdiagnosed as eczema in clinic,including psoriasis,BP,LP,cutaneous amyloidosis,MF and skin malignancies.Misdiagnosis is rather frequent in elderly people and eczematous lesions in genital areas,and pathological examination should be taken actively for uncertain cases.

Objective To explore the relationship of psychological flexibility and the anxiety ,depression in ischemic cerebrovas‐cular disease (ICVD) patients .Methods Two hundred cases of ischemic cerebrovascular disease were collected ,and their anxiety and depression level and psychological flexibility were measured by self‐evaluation of anxiety scale (SAS) ,self‐evaluation of depres‐sion scale (SDS) ,acceptance and action questionnaire‐Ⅱ (AAQ‐Ⅱ ) and cognitive fusion questionnaire (CFQ ) .According to the test results ,the patients with anxiety and depression were divided into different degree :degrees :normal ,mild ,moderate or severe . Results The AAQ‐Ⅱ and CFQ of the patients with anxiety depression were significantly higher than normal emotional ICVD pa‐tients (P<0 .01);there were statistical significance in the difference of the level of AAQ‐Ⅱ and CFQ and the degree of anxiety and depression in these patients (P<0 .01);the level of AAQ‐Ⅱ and the degree of anxiety depression was positively correlated (r=0 .778 ,P<0 .01 ;r=0 .759 ,P<0 .01);the level of CFQ and the degree of anxiety depression was positively correlated (r=0 .785 , P<0 .01 ;r=0 .774 ,P<0 .01);with SAS ,SDS ,as dependent variable ,the AAQ‐Ⅱ ,CFQ as independent variables ,through multiple linear regression analysis ,suggested that psychological flexibility was the impact factor of anxiety depression .Conclusion For pa‐tients with ICVD ,psychological flexibility is closely related to the degree of anxiety depression .Psychological flexibility is one of the predictor of anxiety depression .

Objective To construct miR-223 knockdown lentivirus vector and provide a tool for further study of the function of miR-223. Methods According to the Invitrogen miR-RNAi online design tool, a pair of complementary oligo?nucleotides encoding miR-223 mature sequence was designed, annealed and ligated with pcDNA6.2-GW/EmGFP-miR vec?tor. Then miR-RNAi expression cassette was cut and subcloned into lentiviral pCDH-CMV-MCS-EF1-copGFP vector. The lentiviruses were packaged and titered, and then ST2 cells were infected with viruses. The efficiency of infection was calcu?lated, and the knockdown of endogenous miR-223 was detected by using real-time RT-PCR. Results Restriction enzyme digestion and sequencing results showed that miR-223 lentivirus construct was successfully made. Lentivirus that knock?down miR-223 expression packaged and infected of target cells. The expression of GFP green fluorescent protein accounted for 80%-90%and the virus titer was 1×109 PFU/mL. The infection efficiency reached 90%. Compared with negative control virus, miR-223 knockdown lentivirus significantly down-regulated the expression of miR-223 in ST2, and was 31%(n=3, t=15.091, P<0.05). Conclusion miR-223 knockdown lentivirus is successfully made. It provides a tool for further studying the function of miR-223.

Objective To assess the efficacy of cardiac resynchronization therapy (CRT) using real-time three-dimen?sional echocardiography (RT-3DE) and speckle tracking imaging (STI) in patients with heart failure. Methods Thirty-one patients with chronic heart failure were examined by RT-3DE and STI before CRT and 3, 6 months after CRT. The values of left ventricular end-diastolic volume (LVEDV), end-systolic volume (LVESV) and left ventricular ejection fraction (LVEF) were measured. RT-3DE was used to evaluate the minimum regional volume for 16 segments (Tmsv-16SD) and the ratio of Tmsv16SD to R-R interval, and the maximum time difference (Tmsv-16Dif). STl was used to evaluate the ratios of standard deviation of the time to peak longitudinal radial and circumferential strain to R-R interval (Tls-16SD%, Trs-16SD%,Tcs-16SD%) from the beginning of Q wave of electrocardiography to the point minimal systolic volume of all 16 segments and the maximum time difference. Results The values of LVEDV and LVESV were smaller 3 and 6 months after CRT than those before CRT. The value of LVEF was significantly improved after CRT (P < 0.05). The values of Tmsv-16SD%, Tmsv-16Dif%, Tls-16SD%, Trs-16SD%, Tcs-16SD%and T-Dif%were gradually decreased after CRT (P<0.05). The values of Tmsv-16SD%, Tmsv-16Dif%,Trs-16SD%and Tcs-16SD%were negatively related with LVEF (r=-0.79,-0.82,-0.69 and-0.74(P<0.05). The better consistency was found between RT-3DE and STI methods(Kappa=0.77). Conclusion Re?al-time three-dimensional echocardiography and speckle tracking imaging can provide more accurate quantitative informa?tion of cardiac resynchronization therapy for assessment of treatment and prognosis in patients with heart failure.