Animals ; Cytidine ; analogs & derivatives ; pharmacology ; DNA Methylation ; drug effects ; DNA Modification Methylases ; metabolism ; Gene Knockout Techniques ; Histone Acetyltransferases ; metabolism ; Histone Deacetylase Inhibitors ; pharmacology ; Humans ; Hydroxamic Acids ; pharmacology ; Neoplasms ; genetics ; physiopathology ; therapy ; RNA Interference ; Valproic Acid ; pharmacology

Objective To develop an informatized continuous education system for theater nurse based on PDCA theory.Methods The system was gifted with the functions of learning planning, learning execution, learning checking, data management and system management based on PDCA theory, informatized technology and other learning systems.Results The system behaved well in overall flow and configuration, man-machine interaction and application, which facilitated the nurses to learn with fragmented time.Conclusion The nursing continuous education is enhanced greatly with the system.

Cancer stem cell hypothesis has become a new theory. According to this theory, cancer stem cells play a decisive role in car-cinogenesis, progression, and metastasis. This theory offered new ideas and strategies for cancer treatment. However, only a small fraction of cells in a tumor were cancer stem cells. Therefore, the identification and enrichment of these cells are significant challenges for numerous researchers conducting further study. Micro-sphere culture has been an effective tumor stem cell enrichment technolo-gy in recent years. The application of this technique makes the cancer stem cell research process easy. In addition, it makes the evalua-tion of tumor progression and prognosis of patients in clinical possible. This article will focus on the applications of this technology, and microsystem integration will be described in detail.

Objective To explore the contribution of endoplasmic reticulum stress to cardiac myocyte apoptosis in mouse congestive heart failure induced by Doxorubicin.Methods Doxorubicin (DOX)was administered intraperitoneally to rats,2 mg/kg doses over 8 weeks.Echocardiographic and hemodynamics measurements were obtained at 8 weeks after treatment.Then left ventricular (LV) samples were collected.The successfully established rats were divided into 3 groups (Fu Shen Ke Li 40 mg/kg,60 mg/kg,and 80 mg/kg).After 5 weeks post the operation,an echocardiographic was performed on all the rats under narcotism,Western blot to test the B-cell lymphoma-2 (Bcl-2),myeloid cell leukemia-1 (Mcl-1),Bcl-2-Associated X (Bax),Caspase-3,C/EBP homologous protein (CHOP),and 78-kDa glucose-regulated protein(GRP78),and reverse transcription-polymerase chain reaction (RT-PCR) to test the mRNA expression levels of Mcl-1,and NOXA.Results The left ventricular end-diastolic diameter (LVEDD),left ventricular end systolic diameter (LVESD),left ventricular ejection fraction (LVEF) prompted modeling success The left ventricular end-diastolic diameter (LVEDD),left ventricular ejection fraction (LVEF),and fractional shortening (FS) have been improved in high and middle groups of Fu Shen Ke Li.Compared to the model group,higher expression levels of Bcl-2,and Mcl-1 were,lower expressions of Bax,NOXA,CHOP,and GRP78 were (P ＜ O.05) in 3 groups of Fu Shen Ke Li.Compared to the model group,there were higher mRNA expression level of Mcl-1 was,lower mRNA expression of NOXA was (P ＜ 0.05) in 3 groups of Fu Shen Ke Li.Conclusions Fu Shen Ke Li depresses endoplasmic reticulum stress,which can reduce the apoptosis of myocardial cells,and ultimately achieve the purpose of treatment of heart failure.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Aged ; Catgut ; utilization ; Female ; Humans ; Male ; Middle Aged ; Sleep Initiation and Maintenance Disorders ; therapy

To investigate the relationship between endothelium dysfunction and experimental vasospasm, twenty-eight New Zealand white rabbits were randomly allocated into three groups: sham operation control group(Group 1, n=8), balloon endothelial denudation + normal diet group (Group 2, n=8) and balloon endothelial denudation + hypercholesterol diet group (Group 3, n=12). Angiography was performed to detect the vasospasm induced by ergonovine and endothelium-dependent vasodilator response induced by acetylcholine before and immediately after denudation and 8 week later. Visible vasospasm was induced at the denuded sites in group 3, and endothelium dysfunction was also found in the same locations. Positive correlation was showed between vasospasm and endothelium dysfunction. Endothelium dysfunction resulted from balloon endothelial denudation and hypercholesterolemia may play an important role in the pathogenesis of experimental vasospasm.

Several reconstruction techniques are available after gastrectomy. Roux-en-Y reconstruction following distal gastrectomy is the reference in terms of long-term functional and endoscopic outcomes. It is the preferred reconstruction for benign lesions and early gastric cancer. In patients with advanced gastric cancer, BillrothII (reconstruction is an acceptable alternative. After total gastrectomy, Roux-en-Y reconstruction is the simplest solution, with satisfactory functional outcome. Addition of a jejunal reservoir seems to improve long-term outcome after total gastrectomy and could be of benefit to patients with good prognosis. After distal or total gastrectomy, hand-sewn anastomoses should be preferred because of lower costs. Mechanical sutures can facilitate transhiatal esophagojejunostomy. After proximal gastrectomy, esophago-gastric anastomosis is the basic reconstruction method. Gastric remnant is made into gastric tube in the operation. The effect of pyloroplasty remains controversial, and further study is needed to improve the quality of life after operation.
Anastomosis, Roux-en-Y ; methods ; Gastrectomy ; Gastroenterostomy ; methods ; Humans ; Reconstructive Surgical Procedures ; methods ; Stomach Neoplasms ; surgery

Animals ; Chronic Disease ; Disease Models, Animal ; Female ; Gene Expression Regulation, Developmental ; Hyperoxia ; Immunohistochemistry ; Lung ; growth & development ; metabolism ; Lung Diseases ; metabolism ; Oxygen ; Pregnancy ; RNA, Messenger ; metabolism ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Transforming Growth Factor beta1 ; genetics ; metabolism

Objective To screen the proteins interacting with AMP-activated protein kinase ?2(AMPK?2)in rat brain cDNA library by bacterial two-hybrid system,and to investigate the biological role and the regulatory mechanism of AMPK?2 in brain.Methods The recombinant pBT-AMPK?2 was used as the bait to screen a rat fetal brain cDNA library by bacterial two-hybrid system.The plasmids of positive colonies were extracted and analyzed by DNA sequencing and BLAST search in GenBank.Results Seven proteins binding to AMPK?2 were screened out,including polyubiquitin,small heat shock protein 8,phosphofructokinase,cytochrome C oxidase subunit Ⅰ(COXⅠ),HLA-B-associated transcript 3(BAT3)isoform 1,protein tyrosine phosphatase receptor type D(Ptprd)and islet-brain 1(IB1).Conclusion Polyubiquitin,HSP8,phosphofructokinase,COXⅠ,BAT3 isoform 1,Ptprd and IB1 can interact with AMPK?2 in brain.

Objective To observe the relation of estradiol-stimulated protein (esp1) expression with chromosome ploidy in A549 and Hela cells. Methods Total RNA was extracted from A549 and Hela cells respectively for cDNA. pIRE-S2-EGFP-ESP1 plasmid was transfected into the 2 corresponding tumor cells. Real-time quantitative PCR was used to detect esp1 gene expression. The chromosome changes were analyzed by VIDEOTEST-KARYO 3.1 and chromosome ploidy was analyzed with flow cytometry. Results After the tumor cells were transfected with pIRE-S2-EGFP-ESP1 plasmid, the expression of esp1 gene was up-regulated (P