1.Recent advances in DNA demethylation in tumor therapy.
Chinese Journal of Pathology 2009;38(5):355-357
Animals
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Cytidine
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analogs & derivatives
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pharmacology
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DNA Methylation
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drug effects
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DNA Modification Methylases
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metabolism
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Gene Knockout Techniques
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Histone Acetyltransferases
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metabolism
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Histone Deacetylase Inhibitors
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pharmacology
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Humans
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Hydroxamic Acids
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pharmacology
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Neoplasms
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genetics
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physiopathology
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therapy
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RNA Interference
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Valproic Acid
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pharmacology
2.Micro-sphere culture gathers cancer stem cell and its potential clinical application
Chinese Journal of Clinical Oncology 2017;44(3):136-141
Cancer stem cell hypothesis has become a new theory. According to this theory, cancer stem cells play a decisive role in car-cinogenesis, progression, and metastasis. This theory offered new ideas and strategies for cancer treatment. However, only a small fraction of cells in a tumor were cancer stem cells. Therefore, the identification and enrichment of these cells are significant challenges for numerous researchers conducting further study. Micro-sphere culture has been an effective tumor stem cell enrichment technolo-gy in recent years. The application of this technique makes the cancer stem cell research process easy. In addition, it makes the evalua-tion of tumor progression and prognosis of patients in clinical possible. This article will focus on the applications of this technology, and microsystem integration will be described in detail.
3.Effects of Fu Shen ke li on myocardial cell apoptosis and endoplasmic reticulum stress-related protein expressions in rats with adriamycin induced heart failure
Journal of Chinese Physician 2017;19(4):556-559
Objective To explore the contribution of endoplasmic reticulum stress to cardiac myocyte apoptosis in mouse congestive heart failure induced by Doxorubicin.Methods Doxorubicin (DOX)was administered intraperitoneally to rats,2 mg/kg doses over 8 weeks.Echocardiographic and hemodynamics measurements were obtained at 8 weeks after treatment.Then left ventricular (LV) samples were collected.The successfully established rats were divided into 3 groups (Fu Shen Ke Li 40 mg/kg,60 mg/kg,and 80 mg/kg).After 5 weeks post the operation,an echocardiographic was performed on all the rats under narcotism,Western blot to test the B-cell lymphoma-2 (Bcl-2),myeloid cell leukemia-1 (Mcl-1),Bcl-2-Associated X (Bax),Caspase-3,C/EBP homologous protein (CHOP),and 78-kDa glucose-regulated protein(GRP78),and reverse transcription-polymerase chain reaction (RT-PCR) to test the mRNA expression levels of Mcl-1,and NOXA.Results The left ventricular end-diastolic diameter (LVEDD),left ventricular end systolic diameter (LVESD),left ventricular ejection fraction (LVEF) prompted modeling success The left ventricular end-diastolic diameter (LVEDD),left ventricular ejection fraction (LVEF),and fractional shortening (FS) have been improved in high and middle groups of Fu Shen Ke Li.Compared to the model group,higher expression levels of Bcl-2,and Mcl-1 were,lower expressions of Bax,NOXA,CHOP,and GRP78 were (P < O.05) in 3 groups of Fu Shen Ke Li.Compared to the model group,there were higher mRNA expression level of Mcl-1 was,lower mRNA expression of NOXA was (P < 0.05) in 3 groups of Fu Shen Ke Li.Conclusions Fu Shen Ke Li depresses endoplasmic reticulum stress,which can reduce the apoptosis of myocardial cells,and ultimately achieve the purpose of treatment of heart failure.
4.Study on theater nurse learning system based on PDCA theory
Chinese Medical Equipment Journal 2015;36(5):66-69
Objective To develop an informatized continuous education system for theater nurse based on PDCA theory.Methods The system was gifted with the functions of learning planning, learning execution, learning checking, data management and system management based on PDCA theory, informatized technology and other learning systems.Results The system behaved well in overall flow and configuration, man-machine interaction and application, which facilitated the nurses to learn with fragmented time.Conclusion The nursing continuous education is enhanced greatly with the system.
6.ROLE OF ENDOTHELIUM DYSFUNCTION IN EXPERIMENTAL VASOSPASM
Qiang FU ; Xiangyang FU ; Xin CHEN
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
To investigate the relationship between endothelium dysfunction and experimental vasospasm, twenty-eight New Zealand white rabbits were randomly allocated into three groups: sham operation control group(Group 1, n=8), balloon endothelial denudation + normal diet group (Group 2, n=8) and balloon endothelial denudation + hypercholesterol diet group (Group 3, n=12). Angiography was performed to detect the vasospasm induced by ergonovine and endothelium-dependent vasodilator response induced by acetylcholine before and immediately after denudation and 8 week later. Visible vasospasm was induced at the denuded sites in group 3, and endothelium dysfunction was also found in the same locations. Positive correlation was showed between vasospasm and endothelium dysfunction. Endothelium dysfunction resulted from balloon endothelial denudation and hypercholesterolemia may play an important role in the pathogenesis of experimental vasospasm.
7.Pay attention to digestive tract reconstruction after curative resection of gastric cancer.
Chinese Journal of Gastrointestinal Surgery 2013;16(2):104-108
Several reconstruction techniques are available after gastrectomy. Roux-en-Y reconstruction following distal gastrectomy is the reference in terms of long-term functional and endoscopic outcomes. It is the preferred reconstruction for benign lesions and early gastric cancer. In patients with advanced gastric cancer, BillrothII (reconstruction is an acceptable alternative. After total gastrectomy, Roux-en-Y reconstruction is the simplest solution, with satisfactory functional outcome. Addition of a jejunal reservoir seems to improve long-term outcome after total gastrectomy and could be of benefit to patients with good prognosis. After distal or total gastrectomy, hand-sewn anastomoses should be preferred because of lower costs. Mechanical sutures can facilitate transhiatal esophagojejunostomy. After proximal gastrectomy, esophago-gastric anastomosis is the basic reconstruction method. Gastric remnant is made into gastric tube in the operation. The effect of pyloroplasty remains controversial, and further study is needed to improve the quality of life after operation.
Anastomosis, Roux-en-Y
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methods
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Gastrectomy
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Gastroenterostomy
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methods
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Humans
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Reconstructive Surgical Procedures
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methods
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Stomach Neoplasms
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surgery
8.Diagnosis and treatment of testicular torsion
Jincao FU ; Xin GOU ; Mingchao XIAO
Journal of Third Military Medical University 2003;0(09):-
Objective To investigate the reason why the misdiagnostic rate of testicular torsion is high and improve its diagnosis and treatment.Methods The clinical data of 20 cases of testicular torsion were retrospectively analysed.Results All 20 cases had the symptom of sudden colic of scrotum and went to hospital in 2 h.Only 5 cases were diagnosed as testicular torsion in 6 h by color Dopple ultrasonography.Three cases were cured by surgical detorsion and the testicles of 17 cases were removed for necrosis.The other testicle of all 20 cases was fixed.Conculusion The early diagnosis of testicular torsion is the most important and color Dopple ultrasonography is a reliable easy way.The sufficient knowledge of this disease by the doctors is crucial in improving the diagnosis and treatment.
9.A comparative study of choledocholithotomy by open and laparoscopic surgery
Zhuangwen ZHANG ; Xin FU ; Yuanjian LI
Chinese Journal of General Surgery 1993;0(02):-
Objective To compare the clinical results of choledocholithotomy by laparoscopic surgery and open surgery.Methods One hundred and Sixty-nine patients with cholecystoithiasis combined with choledocholithiasis were divided into laparoscopic surgery group(85 cases of laparoscopic choledocholithotomy,LCD-TD) and open surgery group(84 cases of open choledocholithotomy,OCH-TD).The clinical data in the two groups were compared and analyzed.Results The amount of intraoperative bleeding,the recover time of intestinal peristalsis after treatment,length of hospital stay,cases of postoperative pain and biliary fistulas in laparoscopic surgery group were evidently better than those of open surgery group(all P
10.Difference between the bone marrow and peripheral blood engraftment evidence after allogeneic hematopoietic stem cell transplantation by PCR-STR
Wenjie XIA ; Yongshui FU ; Xin YE
Chinese Journal of Blood Transfusion 2001;0(06):-
Objective To observe the difference between the bone marrow and peripheral blood engraftment evidence after allogeneic haemopoietic stem cell transplantation(Allo-HSCT) using PCR-STR. Methods DNA from peripheral blood or bone marrow of donors and recipients in different phases were extracted,and 16 STR loci with high polymorphism were amplified by PCR.Separation of the PCR products and fluorescence detection were performed by ABIprism 3100 Genetic Analyzer with capillary electrophoresis.Results The 16 patients included in the study had different levels of engraftment.Twelve patients displayed complete chimerism,while 5 patients showed mixed chimerism.One patient was keeping continuance of remission.The decrease of donor DNA amounts in mixed chimerism was earlier in bone marrow than that in peripheral blood(P