Animals ; Cytidine ; analogs & derivatives ; pharmacology ; DNA Methylation ; drug effects ; DNA Modification Methylases ; metabolism ; Gene Knockout Techniques ; Histone Acetyltransferases ; metabolism ; Histone Deacetylase Inhibitors ; pharmacology ; Humans ; Hydroxamic Acids ; pharmacology ; Neoplasms ; genetics ; physiopathology ; therapy ; RNA Interference ; Valproic Acid ; pharmacology

Objective To develop an informatized continuous education system for theater nurse based on PDCA theory.Methods The system was gifted with the functions of learning planning, learning execution, learning checking, data management and system management based on PDCA theory, informatized technology and other learning systems.Results The system behaved well in overall flow and configuration, man-machine interaction and application, which facilitated the nurses to learn with fragmented time.Conclusion The nursing continuous education is enhanced greatly with the system.

Cancer stem cell hypothesis has become a new theory. According to this theory, cancer stem cells play a decisive role in car-cinogenesis, progression, and metastasis. This theory offered new ideas and strategies for cancer treatment. However, only a small fraction of cells in a tumor were cancer stem cells. Therefore, the identification and enrichment of these cells are significant challenges for numerous researchers conducting further study. Micro-sphere culture has been an effective tumor stem cell enrichment technolo-gy in recent years. The application of this technique makes the cancer stem cell research process easy. In addition, it makes the evalua-tion of tumor progression and prognosis of patients in clinical possible. This article will focus on the applications of this technology, and microsystem integration will be described in detail.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Aged ; Catgut ; utilization ; Female ; Humans ; Male ; Middle Aged ; Sleep Initiation and Maintenance Disorders ; therapy

Objective To explore the contribution of endoplasmic reticulum stress to cardiac myocyte apoptosis in mouse congestive heart failure induced by Doxorubicin.Methods Doxorubicin (DOX)was administered intraperitoneally to rats,2 mg/kg doses over 8 weeks.Echocardiographic and hemodynamics measurements were obtained at 8 weeks after treatment.Then left ventricular (LV) samples were collected.The successfully established rats were divided into 3 groups (Fu Shen Ke Li 40 mg/kg,60 mg/kg,and 80 mg/kg).After 5 weeks post the operation,an echocardiographic was performed on all the rats under narcotism,Western blot to test the B-cell lymphoma-2 (Bcl-2),myeloid cell leukemia-1 (Mcl-1),Bcl-2-Associated X (Bax),Caspase-3,C/EBP homologous protein (CHOP),and 78-kDa glucose-regulated protein(GRP78),and reverse transcription-polymerase chain reaction (RT-PCR) to test the mRNA expression levels of Mcl-1,and NOXA.Results The left ventricular end-diastolic diameter (LVEDD),left ventricular end systolic diameter (LVESD),left ventricular ejection fraction (LVEF) prompted modeling success The left ventricular end-diastolic diameter (LVEDD),left ventricular ejection fraction (LVEF),and fractional shortening (FS) have been improved in high and middle groups of Fu Shen Ke Li.Compared to the model group,higher expression levels of Bcl-2,and Mcl-1 were,lower expressions of Bax,NOXA,CHOP,and GRP78 were (P ＜ O.05) in 3 groups of Fu Shen Ke Li.Compared to the model group,there were higher mRNA expression level of Mcl-1 was,lower mRNA expression of NOXA was (P ＜ 0.05) in 3 groups of Fu Shen Ke Li.Conclusions Fu Shen Ke Li depresses endoplasmic reticulum stress,which can reduce the apoptosis of myocardial cells,and ultimately achieve the purpose of treatment of heart failure.

To investigate the relationship between endothelium dysfunction and experimental vasospasm, twenty-eight New Zealand white rabbits were randomly allocated into three groups: sham operation control group(Group 1, n=8), balloon endothelial denudation + normal diet group (Group 2, n=8) and balloon endothelial denudation + hypercholesterol diet group (Group 3, n=12). Angiography was performed to detect the vasospasm induced by ergonovine and endothelium-dependent vasodilator response induced by acetylcholine before and immediately after denudation and 8 week later. Visible vasospasm was induced at the denuded sites in group 3, and endothelium dysfunction was also found in the same locations. Positive correlation was showed between vasospasm and endothelium dysfunction. Endothelium dysfunction resulted from balloon endothelial denudation and hypercholesterolemia may play an important role in the pathogenesis of experimental vasospasm.

Several reconstruction techniques are available after gastrectomy. Roux-en-Y reconstruction following distal gastrectomy is the reference in terms of long-term functional and endoscopic outcomes. It is the preferred reconstruction for benign lesions and early gastric cancer. In patients with advanced gastric cancer, BillrothII (reconstruction is an acceptable alternative. After total gastrectomy, Roux-en-Y reconstruction is the simplest solution, with satisfactory functional outcome. Addition of a jejunal reservoir seems to improve long-term outcome after total gastrectomy and could be of benefit to patients with good prognosis. After distal or total gastrectomy, hand-sewn anastomoses should be preferred because of lower costs. Mechanical sutures can facilitate transhiatal esophagojejunostomy. After proximal gastrectomy, esophago-gastric anastomosis is the basic reconstruction method. Gastric remnant is made into gastric tube in the operation. The effect of pyloroplasty remains controversial, and further study is needed to improve the quality of life after operation.
Anastomosis, Roux-en-Y ; methods ; Gastrectomy ; Gastroenterostomy ; methods ; Humans ; Reconstructive Surgical Procedures ; methods ; Stomach Neoplasms ; surgery

BACKGROUND: Conservative treatment receives poor results in treating humeral intercondylar fractures,due to the over-long time fixation,and the recovery of joint function would be restricted.Early functional exercises should be based on stable internal fixation.OBJECTIVE: To explore the effectiveness of bone plate internal fixation in treating humeral intercondylar fractures under AO principle.METHODS: Totally 37 cases with humeral intercondylar fractures at the Department of Orthopaedics,Laibin People's Hospital from June 2000 to November 2005 were collected,under AO/ASIF classification,cases were assigned into three groups: type C1consisted of 11 cases,C2 consisted of 21 cases and C3 comprised 5 cases.All the cases were treated by olecranon osteotomy medial approach open reduction and then treated with dual anatomical plate internal fixation.Flexion and extension activities were done at 1 day after operation.Physical therapy was performed at 1 day after removing drainage.According to the images and clinical follow-up,slightly resistance exercise could be done at 4 weeks after operation,and gradually increased the load after8-12 weeks.RESULTS AND CONCLUSIONS: A total of 36 patients were included in the follow-up with 13-26 months,mean 19.5 months.All the bone fractures were recovered.Earlier operative reduction and exercise function would got better elbow joint recovery.All results demonstrated that dual anatomical plate treatment under AO principle provides firm internal fixation for patients with humeral intercondylar fractures,which is good for early exercise and functional recovery.

BACKGROUND: Vascular dementia is a syndrome characterized by cognitive impairment, which is induced by ischemic or hemorrhagic apoplexy or ischemic-anoxic brain injury. It is assigned to "imbecility", "dementia", "sluggish consciousness", "susceptible to forget things" etc. By traditional Chinese medicine. Impairments of memory and cognition etc. Caused by dementia seriously affect psychosomatic health and quality of life of elderly people.OBJECTIVE: To observe the effects of shuanggen cephalocathartic decoctum on learning, memory and spatial discrimination ability of vascular dementia rats.DESIGN: Randomized control trail taking experimental animals as objects.SETTING: Teaching and Research Section of Basic Theory of Traditional Chinese Medicine, Preclinical Medicine College, Nanjing University of Traditional Chinese Medicine.MATERIALS: The experiment was performed in Laboratory of Viscerastate of Teaching and Research Section of Basic Theory of Traditional Chinese Medicine, Preclinical Medicine College, Nanjing University of Traditional Chinese Medicine, from January to February 2004. A total of 40 male SD rats were selected and randomly divided into sham operation group, model group, model plus traditional Chinese medicine group and model plus Western medicine group, with 10 in each group. Before the experiment, all the rats were adaptively fed for one week, free drinking and eating before and during the experiment. Medicine and reagent are shuanggen cephalocathartic decoctum and Nimotop tablet, and main experimental apparatuses are DTT-2 jumping apparatus and SMG-2 water maze apparatus.METHODS: Blood was collected from left ventricle under aseptic condi tion, and dried in incubator at 37 ℃, after trituration, it was sifted with sieve of 200μm meshes and embolus was prepared. 1 mg embolus and 0.3 mL physiological saline was mixed and shaken up into suspension. Common carotid artery was temporarily occluded with a vascular clamp, and external carotid artery was retrogradely intubated and the embolus suspension was injected into encephalon to establish model of multiple cerebral infarction. In sham operation group, canulate tubule was intubated only to the bifurcation of common carotid artery, with no fluid injected in. Before the experiment, rats in model group and sham operation group were normally fed, while rats in model plus traditional Chinese medicine group were additionally given shuanggen cephalocathartic decoctum [4 g/(kg·d)] infusion and rats in model plus western medicine group were additionally given Nimotop [2 mg/(kg·d)] infusion, once a day for consecutive 15 days. Passive escape jumping experiment was conducted on rats in each group on the 9th day.Recorded the reaction time for rat jumping onto the rubber cushion after electric shock and the frequency of mistakes of suffering electric shock due to jumping down from the platform in 3 minutes, and took the results as learning indexes. The test was repeated on the 10th day. Put the rat into the box for 3-minture adaptation, then put it on the rubber cushion; recorded the latency for rat first jumping down from the rubber cushion and the frequency of jumpingdown from the rubber cushion in 3 minutes, and took the results as memory indexes. Water maze spatial discrimination experiment was conducted on rats in each group on the 11th day, recorded the frequency of mistakes of rats entering dead end and the latency for landing on safe platform. Every time, took a 40-second rest after swimming. Each rat was trained 10 times a day for consecutive three days. MAIN OUTCOME MEASURES: Results of passive escape jumping experiment and water maze spatial discrimination experiment. RESULTS: Each group had a rat dead in the process of operation except sham operation group in which all rats moved normally. Totally 37 rats entered results analysis. ① Results of passive escape jumping experiment: On the 9th day, compared with model group, the reaction time of rats in sham operation group, model plus traditional Chinese medicine group and model plus western medicine group significantly shortened (P < 0.01 -0.05), the frequency of mistakes in 3 minutes remarkably decreased in sham operation group and model plus traditional Chinese medicine group (P < 0.05); on the 10th day, the latency was significantly prolonged in sham operation group as compared with model group (P < 0.05); compared with model group, the frequency of mistakes in 3 minutes significantly decreased in sham operation group, model plus traditional Chinese medicine group and model plus western medicine group (P < 0.05). ② Results of water maze spatial discrimination: On the 3rd day, compared with model group, the frequency of mistakes of rats entering dead end significantly decreased in sham operation group, model plus traditional Chinese medicine group and model plus western medicine group (P < 0.001-0.05); the frequency of mistakes in model plus traditional Chinese medicine group obvi ously decreased as compared with model plus western medicine group (P < 0.05). During the 2nd and 3rd days of water maze experiment, the time for the rat reaching terminal was significantly shortened in sham operation group, model plus traditional Chinese medicine group and model plus western medicine group as compared with model group (P < 0.05).CONCLUSION: Shuanggen cephalocathartic decoctum can significantly improve learning and memory ability of rat model of vascular dementia, and is more effective than Nimotop in the aspect of improving spatial discrimination ability of rat.

BACKGROUND:Cord tissues,which immune system is relatively non-development with low expression of MHC,rely on the protection of the mother's immune system.Accordingly,studies underlying biological characteristics of umbilical cord mesenchymal stem cells (MSCs) arouse more and more attention.OBJECTIVE:To isolate,separate,and culture MSCs from the tissues of umbilical cord,and to explore the ability of multi-directional differentiation.DESIGN,TIME AND SETTING:The in vitro cytology experiment was performed at the Laboratory of Tissue Engineering of Sichuan University from October 2006 to May 2007.MATERIALS:The umbilical cord of healthy neonate with 37-40 weeks gestational age was obtained from delivery room of the Second West China Hospital,Sichuan University.METHODS:Pieces of umbilical cord were dissected along its long axis,vessel was pulled away,then cord was tied together with a suture creating "loops",then it was placed into collagenase solution,after 6-8 hours,suspended cells obtained from the loops suspension were centrifuged and cultured with adherent method,pass,aged when single cell-derived colonies were appeared.Finally,the 5th generation of cells was induced differentiation towards osteoblasts and lipoblasts.MAIN OUTCOME MEASURES:Morphology,growth,surface antigen expression,as well as the multiple differentiation potential of umbilical cord MSCs.RESULTS:The adherent cells obtained from umbilical cord after removal vessels showed short rod-like or fusiform shape,which can be proliferated and form single cell-derived colonies.Cells isolated from the umbilical cord high expressed integrin markers (CD29,CD51,CD71) but not hematopoietic lineage markers (CD34,CD45 and HLA-DR).These colonies can differentiate into osteoblasts that produce mineralized matrices,stained by alizarin red and alkaline phosphatase,and differentiate adipocytes that accumulate lipid vacuoles and are demonstrated by morphology,stained by oil red.CONCLUSION:Umbilical cord tissue contains a high number of MSC-like elements forming colonies that may be successfully expanded in culture.The proliferation colonies present matrix ceil immunophenotypes,and can differentiate into osteobiasts and adipocytes.