Epigenesis, Genetic ; Humans ; Myelodysplastic Syndromes ; genetics ; therapy

Persicae Ramulus decoction, as the first prescription in Treatise on febrile Diseases, has been recommended by physicians of successive generations. It is also the general prescription for harmonizing yingfen and weifen, yin and yang, qi and blood. Although it only consists of five herbal medicines, it has a wider range of application and more categorized formulas than other prescriptions. Though Persicae Ramulus decoction was originally formulated to treat taiyang apoplexy, it has functions beyond the treatment of exopathic diseases. This formula is also effective in treating internal diseases, surgical diseases, gynecologic diseases, paediatric diseases, etc. KE Yun-bo praised it as the No. 1 formula among ZHANG Zhong-jin's formulas as well as the general prescription for harmonizing Yin and Yang, yingfen and weifen, resolving fleshy exterior and inducing perspiration. Professor SHI Xin-de has been expert at treating intractable diseases by using Persicae Ramulus-associated prescriptions, such as Xiaojianzhong decoction and Baohe pill for children's chronic eczema, Persicae Ramulus and Puerariae Lobatae Radix decoction and Yupingfeng powder for chronic nephritis, and Persicae Ramulus and Longgu Muli decoction for insomnia. Instead of being restricted to Chinese or Western disease names, he prescribed appropriate formulas according to syndromes, thereby achieving a good efficacy.
Adult ; Child ; Drug Combinations ; Drug Prescriptions ; Drugs, Chinese Herbal ; administration & dosage ; Eczema ; drug therapy ; Female ; Humans ; Male ; Middle Aged ; Renal Insufficiency, Chronic ; drug therapy ; Sleep Initiation and Maintenance Disorders ; drug therapy

Objective To discuss the relationships of the genotype of cytochrome P450 2C19 (CYP2C19) gene and and clopidogrel responsiveness.Methods The study enrolled 110 patients with acute myocardial infarction who were treated with clopidogrel in Tiantan Hospital of Beijing from November 2012 to May 2014.Patients were treated by aspirin and clopidogrel.CYP2C19 polymorphisms were detected by genotype testing kits.Platelet inhibition rates were measured by thrombelastography to represent the antiplatelet effect of clopidogrel.The platelet inhibition rates of clopidogrel were compared among different genotypes.Results Compared with carriers of CYP2C19 * 2 or * 3 reduced-function allele,CYP2C19 * 1 wild type had higher platelet inhibition rate of clopidogrel ((35.73 ± 19.29)％ vs.(48.30± 20.84)％),and the difference was significant(t =3.264,P＜0.05).There was no difference between intermediate metabolizer((35.72± 19.27)％) and poor metabolizer((35.75±19.89)％;P＞0.05).The frequency of wild-type homozygotes CYP2C19 * 1/* 1 was higher in responders than in low responders(frequency of low reaction group CYP2C19 * 1/* 1 14 cases (40.0％),* 1/.2 10 cases(28.6％),* 1/* 3 4 cases(11.4％),* 2/* 2 5 cases(14.3％),* 2/* 3 2 cases (5.7％),frequency of reaction group were 45 cases (60.0％),15 cases (20.0％),4 cases (5.3 ％),7 cases (9.3％),4 cases(5.3％);x2 =3.838,P =0.05).Conclusion Polymorphism of gene CYP2C19 is associated with different responses to clopidogrel.CYP2C19 * 2/ * 3 reduced-function allele is associated with low response to clopidogrel.

Environmental Exposure ; adverse effects ; Hearing Loss ; etiology ; Humans ; Noise, Occupational ; adverse effects ; Occupational Diseases ; etiology ; Occupational Exposure ; adverse effects ; Risk Factors ; Smoking ; adverse effects ; Vibration ; adverse effects

Objective To search for the CT and MRI morphological changes in the child patients with cerebral palsy. Method The CT scranning and MRI were carried out in 150 child patients with cerebral palsy, andthe results were analysed. Results In 150 cases, 97 cases (54.6 per cent) have abnormal findings of CT scranning. Diffuse ence-phalatrophy (42 cases), encephalomalacia focus (24 cases) porencephaly (9 cases), and localized ence-phalatrophy (7 cases) ect are the common CT morphological changes in the patients with cerebral palsy.In the 53 patients with normal findings of CT scranning, MRI was carried out for 30 cases and 25 cases(83.3 per cent) were abnormal. The delayed myelinogeny of white matter of brain is the main MRI mor-phalogical abnormality. Conclusion CT scranning and MRI have important value on the etiological diagnosis for child patientswith cerebral palsy. The combination of them can improve the diagnostic accordance rate.

Catheter Ablation ; Humans ; Hypertension ; physiopathology ; surgery ; Kidney ; innervation ; Sympathetic Nervous System ; physiopathology

Calcium ; metabolism ; Growth Substances ; metabolism ; Humans ; Pulmonary Fibrosis ; drug therapy ; metabolism ; prevention & control ; Taurine ; therapeutic use

Objective To measure serum thrombopoietin(TPO)levels in thrombocytopenic conditions of aplastic anemia(AA)and idiopathic thrombocytopenic purpura(ITP)and explore the clinical significance of the changes of TPO levels.Methods Serum TPO concentration was determined in AA(n=13),ITP(n=20)and normal controls(n=17)by using an enzyme linked immunosorbent assay(ELISA).Results Serum TPO level in AA patients was much higher than that in normal controls[(683\^48?414\^73)ng/L vs (99\^41?73\^84)ng/L,P0 05].Furthermore,there was an inverse correlation between TPO concentration and platelet count in AA patients(r=-0\^71,P

To explore the the effect of RSV M 2-1 gene expression on the growth of human lung adenocarcinoma PAa cell line, the recombinant RSV M 2-1 gene eukaryotic plasmid PXJ 41/ M 2-1 was transfected to the human lung adenocarcinoma PAa cell line. Expression of the M 2-1 gene was examined by RT PCR and Western blot. The growth of the human lung adenocarcinoma PAa cell was observed by MTT curve, flow cytometry, the capacity of inherence, colony forming units and inoculation of nude mouse. The results showed that ①The desired fragments of M 2-1 gene were digested by restriction enzyme and RT PCR, respectively. ②The bands of M 2-1 gene protein were found by Western blot. ③ The ratio of transfected PAa cells in S phase of cell cycle was decreased, but increased in G 2 /M phase. Colony forming efficiency and units of the transfected PAa cell were increased, but the capacity of its inherence was decreased. ④Tumorogensising time of the transfected PAa cell in nude mouse was delayed, but its growing speed was increased. The tumor tissue transformed to some extent from adenocarcinoma to squamous carcinoma was found in the nude mouse. It is suggested that the M 2-1 gene may promote the growth of PAa cells, which may implicate some relationship between RSV and lung cancer.