Background Fungal keratitis can cause serious damage to visual function of corneal infective disease,which is more difficult to treat.In recent years,injecting antifungal drugs to the corneal stroma not only enrich the treatment of the disease,but also achieve good clinical effects.Correctly selecting drug kinds and drug concentration can improve the cure rate,and reduce adverse reactions after treatment,but the related research is rare.Objective This study was to observe the clinical effects of corneal stroma fluconazole injection with different concentration for the treatment of fungal keratitis.Methods Prospective study was performed.One hundred and two patients (102 eyes) diagnosed as fungal keratitis were included from May 2012 to January 2015 in Jizhong Energy Xingtai Mining General Hospital.The patients were randomly divided into 3 groups,The eyedrop treatment group (29 eyes) received 0.5％ fluconazole eyedrops and 5％ natamycin eyedrops treatment.The 0.1％ fluconazole group (35 eyes) and 0.2％ fluconazole group (38 eyes) received 0.1％ and 0.2％ fluconazole corneal stroma injection after eyedrop treatment,respectively.Each group underwent potassium hydroxide wet examination and fungal cultures.The curative effect and adverse reactions were observed.Results Fusarium 41.2％ (42/102),Aspergillus 21.6％ (22/102)and Alternaria mold 17.6％ (18/102) ranked the top three pathogenic species.The distribution of pathogenic fungus among the 3 groups were significantly different (x2 =3.763,P＞0.05).The cure rate of eyedrop treatment group was 44.8％ (13/49),which was significantly lower than 0.1％ fluconazole group (74.3％,26/35) and 0.2％ fluconazole group (81.6％,31/38) (x2 =5.782,9.854;both at P＜0.05).The cure rate was significantly different between 0.1％ fluconazole group and 0.2％ fluconazole group (x2=0.566,P＞0.05).The average cure time of eyedrop treatment group,0.1％ fluconazole group and 0.2％ fluconazole group were (36.28 ± 10.39),(29.14± 7.86) and (21.34 ± 8.57) days,respectively,with a significant difference among the three groups (F =5.336,P=0.006).The acuity of vision was significantly increased after treatment in the 0.1％ fluconazole group and 0.2％ fluconazole group (t =3.009,4.695;both at P ＜ 0.01).The average number of injection in the 0.1％ fluconazole group was (5.71 ± 2.97) times,which was higher than (5.13 ± 1.80) times in the 0.2％ fluconazole group (t=4.471,P＜0.05).Four cases in 0.2％ fluconazole group with eye irritation were observed.After diclofenac sodium eyedrops treatment,the symptoms of 3 cases were disappeared.One case was cured by conjunctival flap covering method.All the cases were followed up for 1-3 months,no adverse reactions and recurrence was found.Conclusions Corneal stroma fluconazole injection is an effective method for treatment of fungal keratitis.In order to reduce the occurrence of adverse reactions,0.1％ fluconazole injection in corneal stroma is recommend for light fungal keratitis patients;for moderate and heavy fungal keratitis patients,0.2％ fluconazole injection in corneal stroma can be performed after failure of 0.1％ fluconazole treatment.

Objective To study the role of corn oil in the change of serum glucose, serum lipids and insulin in type 2 diabetes rats with insulin resistance, and attempt to find out its mechanism. Method High fat and sugar diet plus low-dose streptozotocin (STZ) was given to establish type 2 diabetes rat with insulin resistance model, the rats of which were randomly divided into normal group, model group, corn oil (0.8 mg/kg) group, rosiglitazone group. After Ig 6 weeks, fasting blood glucose (FBG), total cholesterol(TC), triglyceride(TG), low density lipoprotein(LDL), high density lipoprotein (HDL), fasting insulin (FINS), and insulin resistance index (HOMA-IR) were detected. Results Corn oil can elevate the level of HDL in diabetic rat, lower the levels of FBG, TC, TG, LDL, FINS and HOMA-IR. Conclusion Corn oil can improve insulin resistance, and decrease the serum glucose and lipid of type 2 diabetic rats.

Objective To observe the protective effect of Mongolian medicine Carthamus tinctorius in acute liver injury induced by D-GlaN(D-galactosamine)in rat models. Methods The acute liver injury model was established by intragastric administration D-GlaN in rats. The levels of serum aspartate aminotransferase(AST),alanine aminotransferase(ALT),glutathione(GSH-Px),the content of tumor necrosis factor-α(TNF-α)in liver tissue and the apoptosis of hepatocytes were determined. Results Mongolian medicine safflower can significantly reduce the activity of serum ALT and AST and liver tissue GSH-Px,and the degree of cell apoptosis;however,no obvious change of the TNF-αcontent was observed. Conclusion The Mongolian medicine Carthamus tinctorius has protective effect in D-GlaN-induced acute liver injury rats probably via anti-oxi-dative and anti-apoptotic abilities.

Enlightened by the essay Affection of Nutrition Factors On Endemic Fluorosis written by professor Li Guang-sheng ,and integrated with the contradictory phenomena in three times epidemic invest igations,the author thought:in epidemic factors of fluorosis,endemic fluorosis not only is related with fluoride intake perday,but also related with nutritional factors,especially calcium in food.The article discussed on countermeasures of prvention of endemic fluorosis in views of the above.

Background and purpose:The epidermal growth factor receptor (EGFR) is commonly overexpressed in a variety of solid tumors, and has important roles in cancer pathogenesis and progression. EGFR thus provides a rational target for cancer therapy. We studied siRNA-mediated inhibition of epidermal growth factor receptor expression and its biologic effects in different human cancer cell lines (A431, HeLa and SPC-A-1). Methods:Cells were transfected with chemically synthesized siRNA-EGFR. EGFR mRNA was quantified by real-time PCR and was detected by immunofluorescence staining and flow cytometry. The biologic effects on cell growth were assessed by colony-formation assay.Results:siRNA-EGFR significantly decreased mRNA level of EGFR by 73.9 %, 44.6 % and 57.7 %, protein expression of EGFR by 77.0 %, 61.3 % and 65.2 %, and reduced colony number by 27.2 %, 53.9 % and 59.1 % in A431, HeLa and SPC-A-1, respectively.Conclusions:Our data suggested that RNA interference could downregulate EGFR and inhibit colony forming ability and EGFR expression at mRNA/protein levels in human cancer cell lines with different pathological types. siRNA could be one of the promising strategies in future targeted cancer therapy.

Objective To study the expression and significance of indoleamine-2,3-dioxygenase(IDO)in non-small-cell lung cancer(NSCLC).Methods Thirty-eight cases of lung cancer tissues and 10 cases of paracancerous normal lung tissue were collected.The semiquantitative immunohistochemistry was used to detect the positive rate of IDO and fluorescent quantitative PCR to detect the level of IDO mRNA in the lung tissues respectively.Results The positive rate of IDO and the level of IDO mRNA in NSCLC group were significantly higher than in paracancerous normal lung tissues(P＜0.05).There was no significant difference in the positive rate of IDO and the level of IDO mRNA between the adenocarcinoma group and the squamous cell carcinoma group(P＞0.05).Statistically,there was significant difference in positive rate of IDO and the level of IDO mRNA among low,middle and high differentiation groups(P＜0.05).There was significant difference in positive rate of IDO and the level of IDO mRNA between the lymph node metastasis and non-metastasis groups(P＜0.05).Conclusion The high expression of IDO in NSCLC is correlated with the degree of differentiation and the metastasis of lymph nodes,but not with the pathological types.

Objective To construct expression vectors that Renilla reniformis (Rluc) fused with neurotensin type 1 receptor (NTSR1),and to investigate the interaction between NTSR1 and other receptors,as well as intercellular signal transduction mechanism mediated by neurotensinl-R.Methods The human NTSR1 gene was amplified by PCR using the plasmid pcDNA3.1-hNTSR1 as template.The PCR product was digested,ligased with the plasmid pRluc and then be transformed into the competent cell Top10.The construct was identified by DNA sequencing.The recombinant plasmid was transiently transfected into human embryonic kidney 293 ( HEK293 )cells,and the expression of pRluc-hNTSR1-pcDNA3.1 was detected by confocal microscopy and Western blot.Results The fragment of 1257 bp was amplified by PCR,and the DNA sequences were identical with the gene in GenBank ( NM_002531 ).Western blot showed a band about 90kDa.Confocal microscopy showed that NTSR1 was expressed on the plasma membrane.Conclusion The pRluc-hNTSR1-pcDNA3.1 eukaryotic expression vector is successfully constructed,and the expression vector can be used to investigate the interaction between NTSR1 and other receptors,as well as intercellular signal transduction mechanism mediated by neurotensinl-R,which will provide new target for drug development.

Objective To investigate the correlation of clinical stage and treatment plan with prognosis in incidental prostatic carcinoma.Methods From 2004 to 2010,a total of 1076 patients diagnosed as BPH underwent transurethral plasma kinetic enucleation of prostate (TUPKEP) in our hospital,and their clinical data were respectively analyzed.Among them,42 cases were found to have incidental prostatic carcinoma.The correlation of clinical stage and treatment plan with prognosis in incidental prostatic carcinoma were observed.Results Among 1076 BPH patients undergoing TUPKEP operation,42 cases (3.9％) aged 56-88 years were found to have incidental prostatic carcinoma,which all displayed as prostatic adenocarcinoma including 18 cases at T1 a stage and 24 cases at T1b stage.10 cases received endocrinal therapy,14 cases received bilateral orchiectomy,14 cases underwent radical prostectomy and 4 cases were treated with watchful waiting.Patients were followed up for 24 to 96 months.The survival rates were 100.0％ and the rates of progression were 0.0％,20.0％,0.0％ in T1 a patients received watchful waiting,androgen-deprivation therapy and radical prostectomy,respectively.Total survival rate was 100.0％ and total rate of progression was 11.1％ in T1a patients.The survival rates were 71.4％,100.0％ and the rates of progression were 42.9％,0.0％ in T1 b patients received androgen-deprivation therapy and radical prostectomy,respectively.Total survival rate was 83.3 ％ and total rate of progression was 25.0 ％ in T1 b patients.Compared with T1 b patients,the total survival rate was higher (x2=18.19,P＜0.01) and the rate of progression was lower in T1 a patients (x2 =6.52,P＜0.05).Conclusions The survival rate in T1 a patients accepted androgen-deprivation therapy is similar to that in T1 a patients with watchful waiting.Compared with T1a patients,the survival rate is lower but the rate of progression is higher in T1 b patients received active androgen-deprivation therapy.The survival rate is higher in T1 b patients treated with radical prostectomy than in those received other treatments.Watchful waiting is acceptable for T1 a patients.T1 b patients should be treated with radical prostectomy for a better effect.

Objective To observe and compare the accurate dynamic changes of types Ⅰ, Ⅲ, Ⅴ, and Ⅵ collagens in the wound healing processes of the rabbit cornea undergoing laser epithelial keratomileusis (LASEK) or photorefractive keratectomy (PRK) and to investigate the possible mechanisms of corneal haze and myopic regression. Methods New Zealand White rabbits were divided into 8 groups: normal control group (n=6), 1, 7 d, and 1, 3, 4, 5, and 6 month groups (n=14). Every rabbit underwent LASEK in one eye while the other one with PRK. We used Western blot analysis to compare the wound healing process of dynamic changes of types Ⅰ, Ⅲ, Ⅴ, and Ⅵ collagens in the rabbit cornea at every time point. The results were analyzed with data analysis software. Results Western blot analysis showed that after LASEK, the cornea wound healing with types Ⅰand Ⅲ collagens were much faster than that of PRK, and the wound response was also much weaker. Whereas for types Ⅴ and Ⅵ collagens, their dynamic changes were similar between LASEK and PRK, and they both reached the peak value at 3 month after the surgery, but LASEK group returned to normal earlier than PRK group. The value of these two types of collagens after PRK was higher than that by LASEK. The changes of these four types of collagens may offer us at least partial explanation to the difference of formation of corneal haze and refractive regression. Conclusion There are significant differences between LASEK and PRK in types Ⅰ, Ⅲ, Ⅴ, and Ⅵ collagens or the time of reacting, reaching apex, and returning to normal. LASEK has slighter intensity of reaction. The results indicate that there is excessive aggradation of collagens after PRK, which may be the histological foundation of obvious haze and myopia regression.

Objective to evaluate the clinical efficacy and safety of ADSCs infusion in patients with type 2 diabetes mellitus(t2DM). Methods Autologous ADSCs were cultured and identified. totally 48 cases of type 2 diabetes patients were divided into two groups:24 cases in A group re-ceived ADSCs and conventional treatment,while the other 24 cases in B group received conventional treatment. With 15 months′follow-up,FPG,2h-PGh,HbA1c,FC-P,AUCC,insulin medication changes and security on post-treatment 3,6,12 and 15 months were calculated. Results the AD-SCs which cultured met the clinical application of standards. FPG,2h-PGh and HbA1c in both groups were significantly decreased,and the values in A group were more impressive than B. there were significant improvement in FC-P,AUCC and insulin of A group compared with B group. there was not treatment related adverse events. Conclusion the clinical data demonstrate that the treatment with ADSCs can improve metabolic control and beta cell function in patients with t2DM. Moreover,the safety of the treatment method was considerable.