Ocular ischemic syndrome(OIS) is a disease seen in cardiology,ophthalmology,neurology and neurosurgery,which is characteristic by brain and ocular symptoms caused by carotid artery obstruction or stenosis.The diagnosis of OIS may be difficult because of its variable presentations and its concealed onset.What is more,its pathogenesis is not completely clear,and therefore its treating efficacy is dissatisfactory so far.The current study on OIS is focused on experimental research.So establishment of suitable animal model is important.Recently years,the creating method of OIS model made a great progress.The selection of model animals,extablishing methods of models and comparison of various models were summarized.

Objective To investigate the low back pain(LBP)of clinic nurses and find out the understanding of related knowledge of LBP in nurses,and improve the occupational protection of nurses.Methods Investigation was carried out in 692 nurses by self-designed questionnaires.Results The incidence of LBP in nurses was 97.4%.The knowledge of LBP and the related occupational protection in nurses were greatly deficient.Conclusions The hospital should pay attention to the protection of LBP in nurses and improve their health.

Background Fungal keratitis can cause serious damage to visual function of corneal infective disease,which is more difficult to treat.In recent years,injecting antifungal drugs to the corneal stroma not only enrich the treatment of the disease,but also achieve good clinical effects.Correctly selecting drug kinds and drug concentration can improve the cure rate,and reduce adverse reactions after treatment,but the related research is rare.Objective This study was to observe the clinical effects of corneal stroma fluconazole injection with different concentration for the treatment of fungal keratitis.Methods Prospective study was performed.One hundred and two patients (102 eyes) diagnosed as fungal keratitis were included from May 2012 to January 2015 in Jizhong Energy Xingtai Mining General Hospital.The patients were randomly divided into 3 groups,The eyedrop treatment group (29 eyes) received 0.5％ fluconazole eyedrops and 5％ natamycin eyedrops treatment.The 0.1％ fluconazole group (35 eyes) and 0.2％ fluconazole group (38 eyes) received 0.1％ and 0.2％ fluconazole corneal stroma injection after eyedrop treatment,respectively.Each group underwent potassium hydroxide wet examination and fungal cultures.The curative effect and adverse reactions were observed.Results Fusarium 41.2％ (42/102),Aspergillus 21.6％ (22/102)and Alternaria mold 17.6％ (18/102) ranked the top three pathogenic species.The distribution of pathogenic fungus among the 3 groups were significantly different (x2 =3.763,P＞0.05).The cure rate of eyedrop treatment group was 44.8％ (13/49),which was significantly lower than 0.1％ fluconazole group (74.3％,26/35) and 0.2％ fluconazole group (81.6％,31/38) (x2 =5.782,9.854;both at P＜0.05).The cure rate was significantly different between 0.1％ fluconazole group and 0.2％ fluconazole group (x2=0.566,P＞0.05).The average cure time of eyedrop treatment group,0.1％ fluconazole group and 0.2％ fluconazole group were (36.28 ± 10.39),(29.14± 7.86) and (21.34 ± 8.57) days,respectively,with a significant difference among the three groups (F =5.336,P=0.006).The acuity of vision was significantly increased after treatment in the 0.1％ fluconazole group and 0.2％ fluconazole group (t =3.009,4.695;both at P ＜ 0.01).The average number of injection in the 0.1％ fluconazole group was (5.71 ± 2.97) times,which was higher than (5.13 ± 1.80) times in the 0.2％ fluconazole group (t=4.471,P＜0.05).Four cases in 0.2％ fluconazole group with eye irritation were observed.After diclofenac sodium eyedrops treatment,the symptoms of 3 cases were disappeared.One case was cured by conjunctival flap covering method.All the cases were followed up for 1-3 months,no adverse reactions and recurrence was found.Conclusions Corneal stroma fluconazole injection is an effective method for treatment of fungal keratitis.In order to reduce the occurrence of adverse reactions,0.1％ fluconazole injection in corneal stroma is recommend for light fungal keratitis patients;for moderate and heavy fungal keratitis patients,0.2％ fluconazole injection in corneal stroma can be performed after failure of 0.1％ fluconazole treatment.

Biomarkers ; Hepatitis B ; therapy ; virology ; Hepatitis B Surface Antigens ; Hepatitis B virus ; Humans

Objective:To study the expression of IQ motif containing GTPase activating protein 1 (IQGAP1 )in oral squamous cell cancer(OSCC)tissue,and to explore the effects of IQGAP1 on cell proliferation and invasion as well as its underlying mechanism. Methods:Expression levels of IQGAP1 in tumor and adjacent normal tissues were examined by western blot and RT-PCR.OSCC cell line SCC-4 cells was transfected with the recombinant plasmid-pcDNA3.1 -IQGAP1 by lipofectamine,and then treated with an Akt in-hibitor.The phosphorylation of Akt,cell proliferation and invasion were detected by western blot,MTT assay and Transwell invasion as-say respectively.Results:Protein and mRNA expression levels of IQGAP1 were higher in cancer tissue than in adjacent normal tissue (P <0.05).Transfection of pcDNA3.1 -IQGAP1 increased IQGAP1 expression,enhanced the capability of cell proliferation and inva-sion (P <0.05),increased p Akt level in the cells.Preconditioning with an Akt inhibitor reduced p Akt level.Furthermore,silencing Akt pathway blocked the increase of cell proliferation and invasion induced by IQGAP1 overexpression(P <0.05).Conclusion:IQ-GAP1 overexprission can mediate the ability of proliferation and invasion of OSCC cells by regulating the activation of Akt pathway.

Objective:To find a better way to protect the security and interests of vulnerable groups by exploring the problem of protecting the vulnerable groups in drug clinical trial from the perspectives of ethics committees,organization and researchers.Methods:According to the relevant literature and the actual situation of the hospital,this paper analyzed the security issues of vulnerable groups comprehensively.Results:Only the ethics committees,organization and researchers work together,can it protect the security and interests of vulnerable groups to the greatest extents.Conclusion:Further research on the security of vulnerable groups not only promotes the development of human health,but also plays a decisive role in improving the protection of subjects in drug clinical trial.

Objective: Based on the same clinical laboratory automatic blood coagulation analyzer method comparison of different brands. Discuss different between blood coagulation analyzer test results are comparable or check whether the deviation of the results in the permitted range. Method:collecting 112 cases of fresh plasma specimens, using ACL ADVANCE and Sysmex CA7000 two kinds of blood coagulation analyzer, respectively prothrombin time (PT), activated partial prothrombin time (APTT), thrombin time (TT) and fibrinogen (FIB), and compare the results, to medical decision level of judging system error detection system for clinical result, and calculate the two blood coagulation instrument testing results of the correlation coefficient and linear regression equation. Results:the ACL ADVANCE and Sysmex CA7000 blood coagulation analyzer test of PT, APTT, TT and FIB result comparison, there were no statistically significant difference (t=0.974, t=0.649, t=0.765, t=1.163;P>0.05), the correlation coefficient respectively;Medical decision level of system error were less than half the total margin of error. Conclusion:to the ACL ADVANCE as standard test system, Sysmex CA7000 determination results have clinical acceptability, two blood coagulation test results with good correlation. Should be conducted on a regular basis in the same laboratory coagulation analyzer compares different experiments, to ensure the comparability of the same in the laboratory test results.

[ ABSTRACT] AIM: To study the influences of tangeretin ( TGN) on the growth and invasion of non-small-cell lung cancer ( NSCLC) cells, and to explore the molecular mechanisms.METHODS: The A549 cells were treated with different concentrations of TGN in vitro.The relative cell activity was determined by MTT assay.The apoptotic rate was an-alyzed by flow cytometry with Annexin V-FITC/PI staining.The number of the invasive cells was measured by Transwell as-say.The mRNA expression of MMP-2 and MMP-9 was detected by RT-PCR, and the protein levels of Ki67, Cyt C, caspase-3, cleaved caspase-3, MMP-2, MMP-9, Akt, p-Akt and p-PI3K were determined by Western blotting analysis. RESULTS:TGN inhibited the proliferation of A549 cells in a dose-dependent manner ( P<0.05) along with the low ex-pression level of proliferation biomarker Ki67.TGN up-regulated the protein levels of Cyt C, caspase-3 and cleaved caspase-3 (P<0.01) and promoted the apoptosis of A549 cells in a dose-dependent manner.Moreover, TGN down-regu-lated the invasion-related molecules MMP-2 and MMP-9 at the mRNA and protein levels, and the number of invasive cells reduced with the increase in the concentration of TGN.The protein levels of p-Akt and p-PI3K in the A549 cells was re-duced (P<0.05), and no difference of the cell viability in the cells treated with different concentrations of TGN was ob-served after blocking PI3K/Akt signaling pathway using LY294002.CONCLUSION: TGN inhibits the growth and inva-sion of A549 cells and promotes the cell apoptosis by potentially inhibiting PI3K/Akt signaling pathway activation.There-fore, this study will provide a new target for the prevention and control of NSCLC.

Neonatal purulent meningitis is one of the severe infectious diseases of the central nervous system.Escherichia coli K1 (E.coli K1) strain is the most common Gram-negative bacteria which leads to neonatal purulent meningitis.The mechanism of E.coli K1 translocation of the blood-brain barrier(BBB) include the high degree of bacteremia,invasion of brain microvessel endothelia cell by E.coli K1,receptor-mediated pinocytosis transport for E.coli K1 and the living bacteria across the BBB.The current knowledge on the mechanisms underlying the successful E.coli K1 translocation of the BBB are summarized in this review.

Fatty acid synthase (FAS) can be regarded as a tumor-specific therapeutic target.High expression of FAS is found in many tumor tissues, and inhibition of its expression can induce tumor cell death.Recent researches has shown that FAS can be used not only as the target of tumor therapy, but also as a tumor marker.The serum FAS is higher in cancer patients.High expression of FAS is associated with tumor treatment tolerance, and some new inhibitors for FAS show good anti-cancer effects.