MircoRNAs (miRNAs) are widely existing inorganisms, non-coding small RNA. MircoR-NAs play important role in cell proliferation, apoptosis, stress resistance and fat metabolism. In vitro studies indicate that miRNAs are important factors in platinum resistance of ovarian cancer cell lines. However, the pathway of platinum resistance of ovarian cancers is complex. Studies find miRNA expression play important roles in the sensitivity of platinum resistance of ovarian cancer cell lines , and can be used as an early alert for platinum resistance. Changing the expression of miRNA even can reverse the platinum resistance . But the regular role of miRNAs in target genes network is still unconfirmed. Thus, more studies about miRNA in vivo and animal tumor models are needed to identify the function of miRNAs in platinum resistance of ovarian cancer.

Objective To investigate the expression and clinical signification of tumor M2 pyruvate kinase in pa-tients with CRC, and to try to find a reliable and atraumatic method to predict the progress and pronosis in the CRC. Methods ELISA method was used to determine the serum levels of tM2-PK in 68 patients with CRC,40 pa-tients with colorectal polyps and 40 cases of normal subjects; immunohistochemistry ( IHC ) methods were respec-tively used to detect the expression of tM2-PK in tissues. Results ① The level of serum tM2-PK in patients with CRC was significantly higher than the group of patients with polyps and the normal subjects ( P<0.05 ) . The serum tM2-PK levels was closely correlated to tumor size and Duke stage.②The positive rate of tissue tM2-PK was signif-icantly higher in CRC than in colorectal polyps and normal subjects ( P<0.05 ) . Tissue tM2-PK levels was closely correlated to tumor size,Duke stage and differentiation grade. ③Serum tM2-PK levels was correlated to tissue tM2-PK levels (r=0.357,P=0.003). Conclusion Combined detection of expression of tM2-PK can provide the basis theory for the diagnosis and prognosis evaluation of CRC.

At present,the main treatment methods of solid tumor are radiotherapy and ehemotherapy.Traditional radiotherapy and chemotherapy have certain limitations because of resistance and toxicity.High dose chemoradiotherpy with hematopoietie stem cell transplantation(HSCT)support can reduce resistance and toxicity,enhance the effectiveness of treatment.But there are still high relapse rate after transplantation and short survival period.How to strengthen the following treatment is worth of further study.This review will focus on the feasibility,treatment time,therapeutic effect and research advancement of HSCT for solid tumor.

Objective To survey the semen quality of healthy adult men in the Liaoning area.Methods We included 400 male volunteers of different ages who underwent a premarital examination and reproductive health checkup in 2015 for semen sample collection.The inclusion criteria were as follows:men who underwent unified semen analysis,those who answered the questionnaire for analysis of overall semen quality,and those who were aged 18-25,26-30,and 31-40 years.Results Among the 400 male volunteers,the consistent standard rate only reached 40.5％ according to the World Health Organization-specified reference values for testicular volume,semen volume,semen concentration,total energy,forward movement,livability,normal appearance,pH,liquefaction time,DNA fragmentation index,and high DNA staining index.The differences between the three groups were statistically significant (P ＜ 0.05).The results of the 18-to 25-year age group were better than those of the other groups.Conclusion Healthy adults' semen quality in the Liaoning area has declined in recent years;therefore,male reproductive health education should be seriously considered.

OBJECTIVE:To observe the efficacy and safety of pidotimod combined with triamcinolone acetonide in the treat-ment of oral lichen planus(OLP). METHODS:Data of 60 patients with OLP were retrospectively analyzed and divided into obser-vation group(35 cases)and control group(25 cases)by different medication. Control group was treated with triamcinolone aceton-ide 1 ml+2% Lidocaine injection 1 ml,embrocated in the local lesion area according to lesion size,3 times a day;observatioh group was additionally treated with Pidotimod dispersible tablets 800 mg between meals based on the treatment of control group, twice a day,and then changed to 800 mg,orally,once a day after 2 weeks. The treatment course was 4 weeks. The clinical effica-cy and incidence of adverse reactions in 2 groups were observed. RESULTS:After 1 and 3 month(s),the total effective rates in ob-servation group were significantly higher than control group,the difference was statistically significant(P<0.05). There was no sig-nificant difference in the incidence of adverse reactions between 2 groups(P>0.05). CONCLUSIONS:Pidotimod combined with triamcinolone acetonide has significant efficacy in the treatment of OLP,with good safety.

Objective:To observe the clinical effect of warm needling moxibustion at Zhongji (CV 3) and Zusanli (ST 36) in treatment of urinary retention after gynecological surgery. ＜br> Methods: Forty patients with urinary retention after gynecological surgery were randomly divided into two groups by the randomized method, 20 cases in each group. Patients in the treatment group were treated by warm needling moxibustion at Zhongji (CV 3) and Zusanli (ST 36). Patients in the control group were treated by inductive urination. The therapeutic effects of the two groups were observed after one course of treatment. ＜br> Results:The total effective rate was 95.0%in the treatment group and 70.0% in the control group. The difference in the total effective rate between the two groups was statistically significant (P＜0.05). ＜br> Conclusion: Warm needling moxibustion at Zhongji (CV 3) and Zusanli (ST 36) is more effective than inductive urination in treating urinary retention after gynecological surgery.

Objective To explore the effects of Qiuxieling associate with Licorzinc therapy on infantile rotavirus enteritis. Methods Infants with rotavirus enteritis were divided into 4 groups (symptomatic and supportive treatment group,Licorzinc group,Qiuxieling group,and Qiuxieling combined with Licorzinc group).The curative effect was analyzed.Results The total ef-fective rates of symptomatic and supportive treatment group,Licorzinc group,Qiuxieling group,and Qiuxieling combined with Licorzinc group were 77.5%,82.5%,85.0% and 92.5%.The average disease courses of symptomatic and supportive treatment group,Licorzinc group,Qiuxieling group,and Qiuxieling combined with Licorzinc group were (6.44 ± 0.79 ),(5.82 ± 0.55 ), (5.66±0.50)and (4.78 ±0.50)days.The curative effects were significantly higher in Qiuxieling combined with Licorzinc group compared to symptomatic and supportive treatment group,Licorzinc group and Qiuxieling group (P <0.05).Conclusion Qiuxiel-ing and Licorzinc were significantly effective in the treatment of infantile rotavirus diarrhea,and can short the disease course.

Objective To analyze the color Doppler ultrasonography of trauma-caused deep venous free-floating thrombus(FFT)of lower limbs,discuss the relations between sonography and the prognosis. Methods Clinical data and color Doppler ultrasound examination results of 130 cases with trauma-caused deep venous FFT of lower limbs were retrospectively analysed.Univariate analysis was conducted to identify the statistically significant factors of patients' prognosis,non-conditional logistic regression analysis was performed to analyse the independent prognostic factors of FFT.Results The non-conditional logistic regression analysis showed that the position of FFT,thrombosis edge definition,floating degree and the volume of detouring blood around the thrombus were independent factors of patients'prognosis(P <0.05). The FFT prognosis of iliofemoral vein was more likely to be fixed than the FFT prognosis of popliteal vein. The FFT prognosis with blurring edges was more likely to be fixed.The more obvious the floating degree of FFT,the higher chance that the FFT prognosis is recanalized.More detouring blood flow around FFT indicated more possibilities that the prognosis could be recanalized.Conclusions The color Doppler ultrasound is able to evaluate the relations between the deep venous free-floating thrombus of lower limbs and prognosis of the patients,which is beneficial for early detection and early treatment,as well as reducing mortality and disability resulted from deep venous thrombus during the orthopaedic perioperative period.

Objective To observe the effect of interleukin-1β(IL-1β) on expression of Matrix Metalloproteinases 13 (MMP-13) in rat chondrocytes and its regulation of miR-27b. Methods Chondrocytes were extracted from 7 Wistar male rats. Expression of MMP-13 were examined by Western blot at 0 h, 24 h, 48 h after IL-1βstimulation. Differential miRNAs expression profiles were examined by miRNAs microarray. The most obviously down-regulated miRNAs were confirmed by quantitative Real-time PCR. Targeted regulation relationship between miR-27b and MMP-13 was set up by Luciferase re?porter gene experiments. Results Expression of MMP-13 in rat chondrocytes was increased at a timely dependent manner upon IL-1βstimulation(P<0.05);Microarray revealed 36 miRNAs whose expression changed, among which 6(miR-27b, miR-31, miR-26a, miR-26b, miR-23, miR-204)were especially obvious. Real-time PCR confirmed that miR-27b was the one whose expression level were most down-regulated. Transient co-transfection of miR-27b mimics with luciferase expres?sion plasmids resulted in significant repression of luciferase activity in rat chondrocytes (P < 0.05). Conclusion IL-1βstimulation result in down-regulation of miR-27b and up-regulation of MMP-13 expression. MiR-27b and MMP-13 show targeted regulation relationship.

With the progress of genetic studies,it has been found that long non-coding RNA( LncRNA) in many regulations which play a key role in development of tumor.Its own individual pieces can also have differ-ent adjustive effects;some LncRNA can even have different regulation and controlling effect in a variety of tumor. How the newly discovered LncRNA expression in colorectal cancer?This review mainly outlines the research de-velopment of LncRNA in colerectal cancer in brief.