1.Recent advances in perioperative pain management in orthopaedic surgery
Orthopedic Journal of China 2009;17(24):1873-1875
It is generally known that postoperative pain can cause many adverse clinical effects on the patients of orthopaedic surgery such as lack of exercises of the involved limb,atrophy of the related muscles,anchylosis,osteoparosis,et al,which will ultimately affect the patient's final recovery and living quality.This paper introduces some recent advanced theories about the orthopaedic postoperative pain diagnosis,evaluation and various kinds of treatments hoping to achieve more effective perioperative analgesia in orthopaedic surgery.
3.Clinical value of Holter monitor for clinical diagnosis and treatment of dilated cardiomyopathy
Chinese Journal of Primary Medicine and Pharmacy 2015;22(2):207-209
Objective To investigate the clinical value of Holter monitor(DCG) in the diagnosis and treatment of dilated cardiomyopathy (DCM).Methods According to NT-proBNP > 2 000pg/mL,left ventricular ejection fraction (LVEF) ≤40%,New York Heart Association (NYHA) rated Ⅲ-Ⅳ,67 patients with DCM were divided into the high risk group and nonhigh risk group.All kinds of arrhythmia patients proportion was compared between the two groups,in order to understand the risk factors of DCM and arrhythmias.Results In high risk group,18 cases had ventricular tachycardia,the incidence rate was 66.7%,which was significantly higher than that of non high risk group (2 cases,5.0%) (x2 =29.271,P < 0.01).While other arrhythmia,atrial premature beats,single premature ventricular contraction,atrial fibrillation and so on,had no statistical difference (P > 0.05).Conclusion The DCM patients with various types of arrhythmia,ventricular tachycardia in patients directly influence the severity and prognosis,Holter monitor has an irreplaceable role.
4.Antagonizing effect of diazepam on fipronil induced acute poisoning.
Zhong-Qiu LU ; Qiao-Meng QIU ; Guo-Xin HE
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(1):39-40
Animals
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Diazepam
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pharmacology
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Drug Antagonism
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Electroencephalography
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Female
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Male
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Pyrazoles
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poisoning
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toxicity
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Rats
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Rats, Sprague-Dawley
6.In vitro isolation and culture of human epidermal stem cells
Yuanlin ZEN ; Guohua XIN ; Zeliang QIU
Chinese Journal of Tissue Engineering Research 2008;12(43):8597-8600
BACKGROUND: How to acquire high-purity epidermal stem cells (ESCs) and maintain ESC phenotype and in vitro proliferation characteristics are urgently solved problems in the in vitro culture of ESCs.OBJECTIVE: To establish methods to in vitro isolate and culture human ESCs.DESIGN,TIME AND SETTING: Cell observation was performed in the Department of Bum,First Affiliated Hospital of Nanchang University.MATERIALS: Foreskin was obtained front 2-12-year-old patients who were subjected to circumcision.These patients did not suffer from urinary system infection.METHODS: Keratinocytes and fibroblasts were isolated from foreskin using Dispase Ⅱ and trypsin.ESCs were isolated by rapidly adhering to type Ⅳ collagen.The supematant fluid of fibroblasts of passages 2-3,which were in the exponential growth phase,was collected.After screening,the supematant fluid was mixed with serum-free medium of keratinocytes at the proportion of 1:1.Simultaneously,fetal bovine serum,epidermal growth factor,and bovine pituitary extract were added to prepare medium of ESCs,which was used for in vitro proliferation of human ESCs.Homologous keratinocytes were used as controls.MAIN OUTCOME MEASURES: After 2 passages,cloning efficiency and cloning sustaining time were calculated by plate clone formation assay.Expression levels of β 1 integrin and keratin 19 were detected by immunohistochemistry.RESULTS: The cloning efficiency of human ESCs was significantly increased,and the cloning sustaining time was significantly prolonged as compared with homologous keratinocytes cultured in the same batch (P < 0.01).lmmunohistochemistry results demonstrated that both β 1 integrin and keratin 19 were positive in the human ESCs.CONCLUSION: Keratinocytes could be successfully isolated by means of rapid adherence to type Ⅳ collagen and cultured with conditioned medium in vitro.
8.Cardiovascularpathology in China.
Chinese Journal of Pathology 2005;34(10):616-618
Animals
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Atherosclerosis
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etiology
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genetics
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pathology
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China
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Coronary Disease
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pathology
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Genes, p53
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Humans
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Point Mutation
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Risk Factors
10.Functional connectivity of EEG's delta-band in epilepsy interictal seizures based on Granger causality analysis
Hongyan ZHANG ; Zhijun QIU ; Xin TIAN
International Journal of Biomedical Engineering 2012;35(2):79-85
ObjectiveThe purpose of this research was to investigate functional connectivity of 16-channel electroencephalograph(EEG) in δ frequency band based on Granger causality analysis.MethodsThe experimental data was recorded at a sampling rate of 200 Hz from temporal lobe epilepsy (TLE) patients(6 left and 3 right TLE,and 9 normals as control group.Ten of EEG segments of 20 s length for three different states:epileptiform discharges (ED) state in interictal durations,non-ED state for TLE patients,and control state for the normal.The δ band components (1~4 Hz) were filtered from EEGs.The functional connection values Iδ between two EEG δ components were calculated separately by Granger causality analysis.The two EEG components were from inferoposterior temporal lobe (left:T5,right:T6) to frontal lobe (Fp1,Fp2,F3,F4,and parietal lobe (C3,C4) for three states.ResultsThe Iδ values for ED state was 0.1323±0.0329~0.1670±0.028 9,which was significantly higher than that of non-ED state (0.0300±0.0130~0.0420±0.0072) (P<0.05).The Iδ values for the control group (0.0153±0.0028~0.0193±0.0057) was much lower than that of ED state (P<0.01),and no obvious distinctions were observed compared with non-ED state at P=0.05 level.ConclusionThere is a stronger connection of EEG' s δ bands from the inferoposterior temporal lobe to frontal and parietal lobe for the ED state,and the over-discharges transmission is from inferoposterior temporal lobe to other brain regions.There is a weaker connection from the top temporal lobe to frontal and parietal lobe for non-ED state and control group,and the onset zones is not inferoposterior temporal lobe.