This paper reviewed the present situation of acupoints and meridians issue from the angle of acupuncture and the infrasonic sound. Researches regarding the acupuncture process are studied from the angle of energy. The lifting-inserting method in acupuncture is considered as a way of inputting energy directly, and entwisting needle is considered as a way of inputting energy indirectly. As a result, the two ways caused meridian material (glue flexible body) oscillating. By the frequency of infrasonic wave, the energy is transmitted through the meridian lines, and the magnifying process is induced. Accordingly, the energy result of the body will be transmitted with acupuncture energy. At last, the dynamics equation and the energy equation analysis are given, from the angles of classic and modern physics, shows that the acupuncture function effect is the combined action effect produced by nervous system which stimulated by infrasonic sound energy.

OBJECTIVE:To investigate the relationship between therapeutic effects of N-acetylcysteine(NAC) on animals with fulminant hepatic failure and contents of plasma amino-acids METHODS:Plasma amino-acids were analysed as their precolumn derivatives with O-phthol-dialdehyde by reversed-phase high-performance liquid chromatographic separation and fluorescence detection RESULTS:NAC could decrease the contents of plasma amino-acids,increase the ratio of BCAA/AAA and decrease the mortality of fulminant hepatic failure animals CONCLUSION:Therapeutic effects of NAC on animals with fulminant hepatic failure were related to its effect on the contents of plasma amino-acids

E2Fs transcription factors can control transcription of genes involved in cellular proliferation,differentiation and apoptosis.So far,in mammals,E2F family has seven identified members,E2F-1,E2F-2,E2F-3,(E2F-4),E2F-5,E2F-6 and E2F-7,and each of has special function.Recently,many investigations have demonstrated that E2F-1 has an apparently unique capability to induce apoptosis,and its functions relate to P53、P73、APAF-1、Caspase3 、Caspase7、BcL-2 family members、DIP、SIVA 、NF-?B factor and so on.According to the dependence on P53,molecular mechanisms of E2F-1 induced apoptosis are classified as both P53-dependent and P53-independent.Therefore,(E2F-1) is a novel target site of tumor genetherapy.

Colorectal neoplasms always present with thickness of the intestinal wall or a soft tissue mass in the enteric cavity. Multi-slice computed tomography (MSCT) with high spatial resolution and advanced post-processing techniques can demonstrate the above signs of the tumor, and the invasive signs of adjacent structures and lymph node metastasis. Combined with three dimensional reformation images, MSCT shows a higher sensitivity than that of double contrast barium enema and electronic colonoscope. MSCT is promising in the diagnosis and preoperative evaluation of colorectal neoplasms.

Objective To study the therapeutic effect of arbutin combined with glycyrrhizin on immune-inflammation. Methods The effect of arbutin combined with glycyrrhizin on adjuvant arthritis and on oxzolone induced mice ear swelling were investigated. PGE2 level in inflammatory tissue and LTB4 content from peritoneal macrophage of rat treated with arbutin and glycyrrhizin were determined. Results Arbutin combined with glycyrrhizin could inhibit Freund's complete adjuvant induced rat primary and secondary foot swelling. The combined group could also inhibit oxazolone induced mice ear swelling and decrease CD4+ T cell in blood. Compared with control group,PGE2 level in combined group was remarkable lower,and there was no difference between combined group and GL 400 mg/kg group. Arbutin combined with glycyrrhizin could inhibit LTB4 release from rat peritoneal macrophage. Conclusion Arbutin and glycyrrhizin has synergetic therapeutic effect on immune-inflammation.

Objective To investigate the role of the classification of Lauren in staging of gastric carcinoma and preoperative assessment using spiral CT. Methods Forty eight patients, 32 men and 16 women, who were 24～78 years old, with histologically and endoscopically proven gastric carcinoma, underwent spiral CT scans in prone position, with drug induced hypotonia and water filling. To induce gastric wall hypotonia and to maintain it during the entire examination, 10～15 mg of 654 2 was administered iv before basal scanning. All patinets underwent 2D, 3D and axial imaging using spiral CT, and 46 patients underwent dual phase contrast within one breathhold each. All patients with gastric carcinoma underwent staging and preoperative assessment by two radiologists according to the classification of Lauren. Results Spiral CT correctly detected all tumours. In our series, gastric carcinoma was of the diffuse type in 33 cases, of intestinal type in 15 cases; first stage in 2 cases, second stage in 18 cases, third stage in 15 cases and forth stage in 13 cases. There were 40 patients being performed operation, gastrectomy was performed in 33 patients and laparotomy was performed in 7 patients. The staging and preoperative assessment accuracy was 85.4% and 95.0% respectively, overstaging was 10.4%, and understaging was 4.2% according to the classification of Lauren. Correct rate of spiral CT detected lymph nodes (N1,N2) was 74.1% and 82.7% respectively. There are about 18 patienes whose arteries were invaded by tumors. With dual phase contrast, spiral CT can get well in detecting tumour, especially in staging gastric carcinoma in artery phase. Conclusions The classification of Lauren is recommended for staging of gastric carcinoma and preoperative assessment using spiral CT.

Objective To investigate the short-term outcome of vacuum sealing drainage (VSD) in the treatment of infection early after tibiofibular fracture surgery.Methods A retrospective analysis was performed on 19 patients sustaining early infection after tibiofibular fracture surgery from December 2012 to December 2014.The patients consisted of 13 males and 6 females,aged from 26 to 57 years [(37.0 ± 5.3) years].There were 4 cases of isolate tibial fracture,2 isolate fibular fracture,and 13 tibial and fibular fracture.Open fractures were noted in 13 cases and close fractures in 6 cases.Infection occurred 14-21 days [(18.0 ± 3.3) days] after open reduction and internal fixation and was treated with gentamicin and VSD.Parameters were monitored dynamically after operation,including white blood cell count (WBC),C-reactive protein (CRP),erythrocyte sedimentation rate (ESR) and limb perimeter.Results The wound was basically debrided 7-10 days after VSD.Eighteen cases had one-stage wound closure with instrumentation preservance,and one case with progressed infection from soft tissue to bone marrow had instrumentation removal and wound suture.VSD decreased markers of inflammation including WBC,CRP,and ESR as well as limb perimeter.A follow-up of 6-24 month [(11.0 ± 2.3)months] showed fracture healing,without complications of detectable deformity growth,dysfunction,or chronic osteomyelitis.Conclusion VSD results in sufficient wound drain,reduction of swelling,decreased levels in CRP and ESR and controlled wound infection,which facilitates wound closure.

AIM: The effects of selenium dioxide (SeO_2) on proliferation, apoptosis, intracellular reactive oxygen species (ROS) and Ca~(2+) levels in three leukemia cell lines NB4, K562 and HL-60 were investigated. METHODS: Three leukemia cell lines were treated with 3-30 ?mol/L SeO_2. Flow cytometry was used to detect apoptosis rate, and analyze the changes of ROS and Ca~(2+) level within cells. RESULTS: SeO_2 at 10 and 30 ?mol/L inhibited proliferation in three leukemia cell lines. Treatment with 30 ?mol/L SeO_2 for 48 h induced 54.0%, 46.5%, 49.6% apoptosis in NB4, K562, and HL-60 cells, respectively, and also markedly decreased ROS and Ca~(2+) levels among three cell lines. The rate of ROS positive cells in NB4 and HL-60 decreased with the increase in SeO_2 concentrations. ROS was clearly reduced with 30 ?mol/L SeO_2 in K562. Ca~(2+) levels were tardily declined with 10, 30 ?mol/L SeO_2 in NB4 and HL-60 cells. Ca~(2+) levels were clearly reduced with 30 ?mol/L SeO_2 in K562. CONCLUSION: SeO_2 induces apoptosis in three leukemia cells. The declines of intracellular ROS and Ca~(2+) levels are involved in apoptosis induced by SeO_2.

Objective To discuss the clinical effects of internal fixation with pubic nails and sacroiliac screws aided by 3D navigation for pelvic fractures of Tile type B2 or B3. Methods A total of 27 patients with pelvic fracture of Tile type B2 or B3 received operative treatment in our hospital from De-cember 2013 to December 2016. They were 15 men and 12 women, aged from 20 to 68 years ( average, 39. 2 years ) . According to the Tile classification, 12 cases were type B2. 1, 8 type B2. 2, and 7 type B3. Of them, 20 were unilateral pubic rami fractures and 7 bilateral pubic rami fractures; there were 3 dislocations of the sacroiliac joint, 17 fractures of the sacral Denis regionⅠ, and 7 fractures of the sacral Denis regionⅡ. All the 27 cases were treated by internal fixation with retrograde pubic nails and sacroiliac screws aided by 3D navi-gation. Quality of fracture reduction, functional recovery of the pelvis and postoperative complications were recorded to evaluate the clinical effects. Results Totally 26 out of the 27 patients were followed up for 6 to 18 months ( average, 11. 4 months ) , with one lost to the follow-up. According to the Matta scoring, the pelvic reduction was evaluated as excellent in 20, as good in 4 and as fair in 2 cases, yielding an excellent and good rate of 92. 3％. All the fractures united after 12 to 20 weeks ( average, 13. 8 weeks ) . According to the Majeed scoring at the final follow-ups, the pelvic function was evaluated as excellent in 20, as good in 3 and as fair in 3 cases, giving an excellent and good rate of 88. 5％. Screw loosening happened in one patient who had ambulated too early. No permanent pain, wound infection, neurovascular injury, heterotopic ossification or thrombosis occurred. Conclusion For the treatment of pelvic fractures of Tile type B2 or B3, internal fixation with pubic nails and sacroiliac screws aided by 3D navigation can lead to satisfactory short-term outcomes due to advantages of limited operative invasion, short operation time and limited intraoperative blood loss.

BACKGROUND:It is discovered recently that miRNA is a new regulator that is able to have an impact on gene expression and miRNA contributes to proliferation, differentiation and self-renewal of pluripotent stem cels.
OBJECTIVE:To investigate the mechanism by which miR-155 regulates chondrogenic differentiation of bone marrow mesenchymal stem cels.
METHODS: Sixty healthy Sprague-Dawley aged 12 weeks were randomized into study group and control group, with 30 in each group. Under anesthesia, rats were sacrificed to harvest bone marrow of the lower limbs. Then bone marrow mesenchymal stem cels were isolated, cultured, and transfected with miR-155 mimics in the study group and a negative control sequence in the control group. After chondrogenic induction, RT-PCR was used to detect the expressions of Sox9, Colagen II, Aggrecan and Colagen X gene, and western blot assay to detect the expression of Sox9 and Runx2 proteins.
RESULTS AND CONCLUSION:Compared with the control group, the mRNA expressions of Sox9, Colagen II and Aggrecan were higher, but the mRNA expression of Colagen X was lower in the study group (P < 0.05); the protein expression of Sox9 was higher, but the protein expression of Runx2 was lower in the study group (P < 0.05). These findings indicate that miR-155 promotes the chondrogenic differentiation of bone marrow mesenchymal stem cels and moreover, it can suppress the hypertrophy trend of bone marrow mesenchymal stem cels differentiating into chondrocytes.