Binding of ~(125)I-HDL_3 to cultured skin fibroblasts from normal subjects and cffccts of HDL_3 on removal of the cellular cholesterol were studied. The results showed that ~(126)I-HDL_3 binding to HDL receptors did not require divalent cations and was not reduced by trysin treatment. Binding of ~(126)I-HDL_3 was enhanced about twofold (P

Objective: To compare cumulative dose technique with single dose technique for determining dose-re sponse relationship of rocuronium during balanced anesthesia. Method: Thirty-four ASA grade Ⅰ-Ⅱ patients, aged 23- 65 years, undergoing elective surgery,were randomly allocated to single dose group(n=24) or cumulative dose group (n=10). Single dose group was further divided into 4 subgroups receiving 150,200,250 or 300?g/kg of rocuronium re spectively. In cumulative dose group, the initial dose of 150 or 200 ?g/kg rocuronium was followed by a second dose de termined individually to produce about 90% neuromuscular block. The ulnar nerve was stimulated at the wrist to mea sure the accelography of thumb adduction. The relationship between probit-transformed depression of first twitch height of TOF and the logarithm dose of rocuronium was analysed using linear regression. Result: ED_50 and ED_95 of rocuronium were 193.6?69.1 and 367.2?72. 7?g/kg respectively in single dose group, and 213.0?55.1 and 418.5? 61.7?g/kg respectively in cumulative dose group. No statistically significant differences were found in the slope,inter cept,ED_50 and ED_95 values between two groups. Conclusion: The two-dose cumulative technique can be used as effec tively as traditional single dose technique in determining the dose-response relationship of rocuronium.

Semen Ziziphi spinosae ( SZS) , as a commonly used traditional Chinese medicine in clinic for insomnia therapy, is rich in pharmacological active ingredients such as saponins, flavonoids, alkaloids, oil and the other chemical compounds. The recent stud-ies indicated that some active ingredients in SZS exhibited a variety of activities including sedative hypnotics, antianxiety and anti-de-pression by regulating particular neurotransmitters such as 5-hydroxytryptamine (5-HT), gamma-amino butyric acid (GABA), norepi-nephrine, dopamine and glutamate. In accordance with the previous studies on pharmacological activities of SZS, this paper summa-rized and reviewed the applications of SZS in the treatment of central nervous system diseases, myocardial diseases and hepatic disea-ses, which might provide solid evidence for the application development of SZS.

Objective To investigate the correlation between the percentage of systolic stenosis of the mural coronary artery (MCA) and the length and depth of the myocardial bridge using dual-source computed tomography (DSCT). Methods Four hundred and fifty patients suspected of coronary artery disease (CHD) underwent dual-source computed tomography coronary angiography (DSCTCA). The images were analyzed by 2 radiologists independently. When consistency was obtained among the independent results, the diagnosis of MB-MCA could be confirmed. The length of MCA and depth of MB were measured. All data were reconstructed by every 5% R-R interval, the diameters of MCAs during the whole cardiac cycle were reviewed and measured, the phases were detected when the diameters of MCAs were maximal and minimal, the systolic stenosis rate of MCA was calculated,Pearson correlation analysis was used to analyze the relation between length, depth, and degree of systolic stenosis of the MCAs. Results One hundred and ninety-two sites of MB-MCA were found in 163 ( 36. 2% ) of 450 patients. Of the 192 sites, 72 were completely surrounded by myocardium. Among the 30 MCA sites chosen from these 72 sites, the minimal diameters were found at 30%--35% R-R reconstruction interval in 27 sites(90.0% ), and the maximal diameters were found at 70%--80% R-R reconstruction interval in 27 sites (90. 0% ). A significant correlation was not found between systolic stenesis and the length of the MB ( r = 0. 096, P > 0. 05 ) but was found with the depth of the MB ( r = 0. 675, P < 0. 01 ) of the MCA. Conclusion The minimal and maximal diameters of the MCA usually emerged in 30%--35% R-R reconstruction interval and 70%---80% R-R reconstruction interval on DSCTCA, respectively. The degree of systolic stenosis of MCA significantly correlates with MB depth but not length.

Objective To investigate the effects of erythropoietin (EPO) on myocardial apoptosis and protein kinase B (AKT) expression in rats of chronic heart failure (CHF). Methods Thirty male adult SD rats were randomly divided into two groups, sham-operated (Sham) group (n=6) and model (Model) group (n=24). The abdominal aortic coarctation was used to build CHF model. Sixteen survived rats after operation were randomly divided into two groups including EPO group and con-trol (Control) group. EPO group was received 3 000 U/kg EPO intraperitoneal injection 3 times/week for 4 weeks, and Sham group and Control group were received same volume of normal saline. The echocardiography was used to evaluate cardiac function after 4 weeks, 8 weeks and 12 weeks of treatment. After 12 weeks, all rats were sacrificed after 24 h fasting. The cell morphology and myocardial apoptosis were observed, and apoptosis index (AI) was calculated. Myocardial P-AKT/AKT pro-tein expression was detected by Western blot assay. Results Echocardiography showed that ventricular hypertrophy was found in model group after four weeks, heart failure 8 weeks. Compared with Control group, left ventricular ejection fraction (LVEF) was significantly higher after EPO intervention for 4 weeks (P < 0.05), systolic interventricular septum thickness (IVSs), end-systolic left ventricular posterior wall thickness (LVPWs), diastolic interventricular septum thickness (IVSd), af-ter left ventricular end-diastolic wall thickness (LVPWd) were significantly lower (P<0.05). The value of AI was significant-ly lower in EPO group than that of Control group (23.87%±1.45%vs 35.58%±2.81%, P<0.01). The OD value of P-AKT/AKT was significantly decreased in Control group (0.35±0.06) than that of Sham group (0.81±0.17), the value was significant-ly increased in EPO group (1.61±0.16) than that of Control group (P<0.01). Conclusion EPO can improve heart function, inhibit myocardial apoptosis,and promote pro-phosphorylation of AKT in rats with chronic heart failure.

Objective To explore the clinical efficacy of lateral osteotomy via the supra-fibular-head approach combined with lateral locking plating for simple posterolateral tibial plateau fractures.Methods From January 2014 to February 2017,11 patients with simple posterolateral tibial plateau fracture were treated at Department of Orthopaedics,General Hospital of Middle Military Command of PLA.They were 8 males and 3 females,aged from 21 to 55 years (average 41.8 years).In all of them the supra-fibular-head approach was used for lateral osteotomy according to the size and orientation of the fracture block.After the fracture was reset,a full bone graft was implanted under direct visualization,followed by fixation of the posterolateral bone mass with a lateral locking plate which was placed as far as possible above the fibular head.The patients were encouraged 6 weeks after surgery to do knee functional exercise within 90°.The therapeutic efficacy was evaluated at the final follow-up according to the Rasmussen's criteria for knee functions.Results The average operative time was 74 minutes (from 55 to 90 minutes);the average intraoperative blood loss was 70 mL (from 40 to 120 mL).All the patients were followed up for 12 to 35 months (average,17.5 months).No neurovascular injury or incision infection was observed.Bony union was achieved in all patients after 10 to 15 weeks (average,12.5 weeks).Follow-ups found no implant loosening/breakage,genu valgum,genu varum,fracture redisplacement,or knee instability.According to the Rasmussen's function evaluation of the knee joint,10 cases were rated as excellent and one as good.Conclusion Lateral osteotomy via the supra-fibular-head approach combined with lateral locking plating is a preferred treatment for simple posterolateral tibial plateau fractures.

Objective To investigate the characteristics of transient remission of islet function and its influence on long-term prognosis in different onset age patients with type 1 diabetes mellitus(T1DM).Methods 156 newly diagnosed T1DM patients who received treatment and completed follow-up were retrospectively chosen from January 2014 to January 2018.All patients were grouped according to the onset age including childhood group(n=18),adolescent group(n=108),adult group(n=30).The transient remission and retention rate of islet function of three groups were compared.The relationship between onset age and c-peptide level was analyzed.Results BMI,FC-P,and 2 hC-P levels in adolescent group were higher than those in childhood group(P<0.05).The positivity rates of protein tyrosine phosphatase and zinc transporter 8 antibodies in adult group were lower than those in childhood group(P<0.05).The incidence of transient remission of pancreatic function in adolescent group was higher than that in childhood and adult groups(P<0.05).The duration of transient remission of pancreatic function in adolescent and adult groups was lower than that in childhood group(P<0.05).The islet function retention rates in adolescent group after 1 and 4 years from onset were higher than those in childhood and adult groups(P<0.05).Generalized estimation equation analysis showed that the long-term FC-P and 2 hC-P in adolescent group were higher than those in childhood and adult groups(P<0.05).The FC-P and 2 hC-P levels in adult patients with previous transient remission were higher than those in childhood group(P<0.05).Conclusions Adolescent onset T1DM patients are more likely to have transient remission of islet function,while childhood onset patients have longer transient remission of islet function.In addition,the adult T1DM patients had the best long-term islet function in transient remission population.

Objective To evaluate the diagnostic value of prostate imaging reporting and data system version 1 (PI-RADS V1) and version 2 (PI-RADS V2) for detection of prostate cancer (PCa) in the transition zone (TZ).Methods Seventy-seven patients with suspicious lesions in TZ on mpMRI were scored according to the PI-RADS system (V1 and V2) before MR-TRUS fusion guided biopsy prospectively.In all of the patients with suspicious tumors,respectively at least one lesion with a PI-RADS V1 assessment category of ≥3,was selected for biopsy.Independent sample t test was used to compare scores of PI-RADS V1 and V2 between PCa and benign prostatic hyperplasia (BPH).The diagnostic performance of PI-RADS V 1 and V2 for detection of PCa in the transition zone was compared by analyzing ROC basing on the results of MR-TRUS fusion guided biopsy.Results A cohort of 77 patients was performed including 31 cases of PCa (32 cores) and 46 cases of BPH (51 cores).PCa (V1:1 1.50±2.79;V2:4.28±0.99) had significantly higher scores of both PI-RADS V1 and PI-RADS V2 than BPH(V1:7.51± 1.63;V2∶2.61 ±0.67) (P＜0.05).Using a PI-RADS V1 score cut-off ≥ 11,sensitivity and specificity in group PCa and BPH were calculated,which were 68.8％(22/32) and 96.1％(49/51) with a area under curve of 0.869;using a PI-RADS V2 score cut-off ≥4,which were 75.0％ (24/32) and 90.2％ (46/51) with a area under curve of 0.888,respectively.Conclusions PI-RADS system can indicate the likelihood of PCa of suspicious lesions in TZ on Mp-MRI.PI-RADS V2 perform better than V 1 for the assessment of prostate cancer in TZ.

Skeleton metabolic diseases such as osteoporosis and fracture have posed an detrimental impact on the elderly, which is a primary cause of paralysis and even death in patients. Osteoblast and osteoclast are the two major parts in the regulation of bone homeostasis and imbalance of these two cells, which may result in dysfunction of bone metabolism. Recent researches indicated that bone homeostasis was primarily regulated by endocrine, paracrine, and local mechanical processes. However, increasing evidences have indicated that the significant role of nerve system may involve in bone metabolism via both central and peripheral pathways. Neuropeptide Y(NPY), a neurotransmitter that belongs to a family of peptides,serves as a critical hinge connecting nerve system and skeleton system. Several studies have suggested that NPY generated by both central and peripheral nerve system could regulate bone homeostasis and that NPY-energic nerve fibers distributed on bone surface and in intramedullary cavity and NPY receptors located at osteoblast, chondrocyte, and osteocytes also provide a basis for nerve-skeleton metabolic pathways. NPY can directly regulate osteoprogenitor, involving in the production and differentiation of osteoblast and osteoclast. In addition, as a pivotal molecular of energy homeostasis, NPY may affect glucose and fat homeostasis. Studies of animal models also have further indicated energy metabolism may directly or indirectly participate in the regulation of bone mass. Therefore, further researches on the relationship between NPY and bone homeostasis may facilitate to unveil the central and peripheral regulatory effect of NPY on bone homeostasis and provide a new sight for the treatment of skeleton metabolism-related diseases in the future.

Objective:To summarize the imaging characteristics of primary intestinal lymphoma (PIL).Methods:As a cross-sectional study, a total of 103 consecutive patients diagnosed as PIL by pathology were retrospectively enrolled at Affiliated Shandong Provincial Hospital of Shandong First Medical University between January 2013 to December 2023. Ninety-four patients underwent CT plain and enhanced scans, and the remaining 9 patients only underwent MRI examination. Imaging features were evaluated, including the location and shape of the tumor, the shape of the intestinal wall and lumen, abdominal lymph nodes, the density or signal intensity of the tumor, and the degree and mode of enhancement after the enhancement scan.Results:The most frequent location of PIL was the ileum (43.7%, 45/103), followed by the ascending colon (20.4%, 21/103). Ninety-one patients had a single lesion (88.3%), while the other 12 patients had multiple lesions (11.7%). Diffuse large B-cell lymphoma was the most common histopathological type of the PIL (65.0%, 67/103), followed by mucosa-associated lymphoid tissue lymphoma (9.7%, 10/103). Generally, PIL classically presented intestinal wall thickening (81.6%, 84/103) and exophytic mass (28.2%, 29/103). Aneurysmal dilatation of the lumen appeared in 19 patients. Nineteen tumors (18.4%) grew out of the lumen, and perforation was shown in 3 patients. Bulky mesenteric or retroperitoneal adenopathy occurred in 28 patients (27.2%). Seventy-two tumors (69.9%) showed homogeneous enhancement, and 75 (72.8%) showed mild-to-moderate enhancement. In DWI images, all 9 tumors showed significant diffusion limitation.Conclusions:The most frequent location of PIL was the ileum. The main imaging characteristics were segmental intestinal wall thickening, which showed mild-to-moderate homogeneous enhancement and restricted diffusion.