Type 2 Diabetes Mellitus(T2DM) is classified as two types according to somatotype,one is obesity or overweight,the other is normal or emaciation.It can also be classifi ed as two types according to symptoms,one is accompanied with typical syndromes(polyuria,polydipsia,polyphagia and weight loss),the other one is not.Generally speaking,the typical syndrome is not obvious among the patients with obesity or overweight,which is coincident with the theory about stuffy and fully sensation in the middle energizer and interior heat mentioned in emperor’s internal classic.So the therapeutic method is regulating the function of the middle energizer,evacuating full and stagnation,resolving phlegm and removing pathogenic heat.The typical syndrome is obvious among the patients with normal bodily form or emaciation,which is according with the traditional theory about defi ciency of yin and dryness-heat.So the therapeutic method is nourishing yin and clearing heat.The two types above are absolutely different.

QOL(quality of life) is not only one of the most important parts among the four clinical therapeutic evaluations,but also the main measurement tool to evaluate the clinical therapeutic efficiency of type 2 Diabetes Mellitus(T2DM).Based on the theory of traditional Chinese medicine,specific QOL assessment scale of TCM for T2DM should be made to evaluate the therapeutic efficiency.The assessment scale is a necessary to study T2DM of TCM.As far as TCM treating T2DM is concerned,it can also provide evidence of clinical therapeutic evaluation.

This anrticle analyses CT manifestation of 44 patients with obstructive jaundice demonstrated by surgical operation and pathological examination. It indicates that aocording to the distention of the biliary ductal system, the assured rate of the diagnosis may be attained to 100%. Aocording to the distensible range of the billiary ductal system, the assured rate of the diagnosis in the obstructive sites is 88.6 %. In combination with the disease history and the obstructive sites and the form changes of the obstructive sites, the assured rate of the diagnosis of the disease, causes is 81. 8%. This paper indicates that the malignant tumor is the main cause of the biliary ductal system obstruction. It aocounts for 72. 7%.

Objective To establish the determination method of Tubeimoside Ⅰ in Jiazhongxiao preparation and serum of rat. Methods RP-HPLC/UV method was used as follows:Diamonsil C18 column (250 mm?4.6 mm, 5 ?m) and Easyguard column, methanol-water (65∶35) as mobile phase with flow rate of 1.0 mL/min, UV detection at 214 nm. Results The mean recovery rates of Tubeimoside Ⅰ in preparation and serum of rat were (103.49?3.49)% and (104.70?4.69)% respectively. The linearity of Tubeimoside Ⅰ was shown in the range of 1.18～88.5 ?g/mL. Tubeimoside Ⅰ in preparation and serum of rat were 995.3 ?g/g and 3.19 ?g/mL. Conclusion The method was simple, reliable and rapid. It’s quite suitable to be used in the analysis of Tubeimoside Ⅰ in Jiazhongxiao preparation and serum of rat.

E2Fs transcription factors can control transcription of genes involved in cellular proliferation,differentiation and apoptosis.So far,in mammals,E2F family has seven identified members,E2F-1,E2F-2,E2F-3,(E2F-4),E2F-5,E2F-6 and E2F-7,and each of has special function.Recently,many investigations have demonstrated that E2F-1 has an apparently unique capability to induce apoptosis,and its functions relate to P53、P73、APAF-1、Caspase3 、Caspase7、BcL-2 family members、DIP、SIVA 、NF-?B factor and so on.According to the dependence on P53,molecular mechanisms of E2F-1 induced apoptosis are classified as both P53-dependent and P53-independent.Therefore,(E2F-1) is a novel target site of tumor genetherapy.

Objective: To discuss the purposes and methods of determining and employing operational intervention levels in preparedness and response for a nuclear emergency. Methods:According to the safety standards and technical reports issued by the International Atomic Energy Agency (IAEA) and combined with specific conditions in China, the operational intervention levels suitable for applying in China are analyzed. Results: The newly published IAEA technical report provided special operational intervention levels for emergency at a light water reactor. The recommended values are practical that our country can use for reference. Conclusion: It is suggested that the related branches for response to nuclear emergency in China establish feasible operational intervention levels by referring to the IAEA reports and taking into account the special conditions of nuclear facilities in China, in order to improve the capability of medical response to nuclear emergency.

BACKGROUND:It is discovered recently that miRNA is a new regulator that is able to have an impact on gene expression and miRNA contributes to proliferation, differentiation and self-renewal of pluripotent stem cels.
OBJECTIVE:To investigate the mechanism by which miR-155 regulates chondrogenic differentiation of bone marrow mesenchymal stem cels.
METHODS: Sixty healthy Sprague-Dawley aged 12 weeks were randomized into study group and control group, with 30 in each group. Under anesthesia, rats were sacrificed to harvest bone marrow of the lower limbs. Then bone marrow mesenchymal stem cels were isolated, cultured, and transfected with miR-155 mimics in the study group and a negative control sequence in the control group. After chondrogenic induction, RT-PCR was used to detect the expressions of Sox9, Colagen II, Aggrecan and Colagen X gene, and western blot assay to detect the expression of Sox9 and Runx2 proteins.
RESULTS AND CONCLUSION:Compared with the control group, the mRNA expressions of Sox9, Colagen II and Aggrecan were higher, but the mRNA expression of Colagen X was lower in the study group (P < 0.05); the protein expression of Sox9 was higher, but the protein expression of Runx2 was lower in the study group (P < 0.05). These findings indicate that miR-155 promotes the chondrogenic differentiation of bone marrow mesenchymal stem cels and moreover, it can suppress the hypertrophy trend of bone marrow mesenchymal stem cels differentiating into chondrocytes.

Objective To compare the clinical effect of digital orthopedic three-dimensional visualization technology combined with image-based computer navigation and simple image-based computer navigation for percutaneous screw fixation of acetabulum anterior column fractures.Methods A retrospectivecase-control analysis was made on 19 cases undergone percutaneous screw fixation of acetabular anterior column fractures under image-based computer navigation from January 2015 to 2016 March.There were 12 males and 7 females,aged from 21 to 66 years (mean,39.3 years).AO fracture classification was A3 type in 17 cases and B1 type in 2.Based on the application of three-dimensional digital programming,the cases were assigned to two groups:group A (n =9),virtual three-dimensional model was reconstructed and the virtual screw were inserted to uninjured side by software Mimics and group B (n =10),patients were only prepared for routine preoperative preparation.Time of anterior column screw insertion,intraoperative bleeding,intraoperative fluoroscopy frequency,fracture reduction and Majeed score were compared between the two groups.Results All cases were followed up for mean 8.4 months (range,3-12 months).There were no significant differences between groups A and group B in iutraoperative bleeding [(14.1 ± 3.0) ml,(15.1 ± 2.2) ml],good to excellent rate of reduction (89％,80％),good to excellent rate of Majeed score (89％,80％) (P ＞ 0.05).Time of anterior column screw insertion [(22.4-± 3.4) min] and intraoperative fluoroscopy frequency [(24.9 ± 3.8)times] in group A were significantly less than those[(29.4 ± 4.5)min,(30.5 ± 5.8)times] in group B (P ＜ 0.05).Conclusion Digital orthopedic three-dimensional visualization technology is associated with shortened time of anterior column screw insertion and reduced intraoperative fluoroscopy frequency,indicating an effective adjuvant technique for percutaneous screw fixation of acetabulum anterior column under navigation.

AIM: To investigate the effects of antagonistic peptide specifically binding to the second extracellular loop of CC chemokine receptor 5 (CCR5) on inflammatory cell infiltration and TNF-α expression in lung tissues of asthmatic mice.METHODS: The asthmatic model of BALB/c mice was induced by ovalbumin (OVA) and the optimal sensitization concentration of OVA was screened.After modeling, the mice were intervened by gradual concentrations of antagonistic peptide via tail-vein injection.The pathocytological analysis and grading were performed in the lung tissues with HE staining.The expression of TNF-α at mRNA and protein levels in the lung tissues was determined by real-time PCR and Western blot.RESULTS: The optimal concentration of OVA was 500 mg/L (0.1 mL) as this concentration of OVA stably induced moderate degree of inflammation in the BALB/c mice.Treatment with different concentrations (1.5 g/L, 2.5 g/L and 3.5 g/L) of antagonistic peptide at 0.2 mL through tail-vein injection inhibited the expression of TNF-α, and markedly reduced the degree of inflammation in the lung tissues.The optimal concentration of antagonistic peptide was 2.5 g/L as the lung inflammation degree in 2.5 g/L group alleviated by 2 grades, and the number of inflammatory cells was also significantly reduced.Moreover, the mRNA expression abundance of TNF-α was nearly decreased by 90%, and the protein expression of TNF-α was decreased by 70% compared with model group.Meanwhile, the use of antagonistic peptide at 2.5 g/L before OVA stimulation confirmed the preventive function to some degree.In this group, the lung inflammation degree alleviated by 1 grade, and the expression of TNF-α at both mRNA and protein levels decreased by nearly 50%.CONCLUSION: The antagonistic peptide of CCR5 effectively inhibits the expression of TNF-α and relieves the inflammation in the asthmatic mouse lung tissues in a concentration-dependent manner.

The inheritance of prestigious TCM doctors' experiences is not only important for improving the level of health protection and developing the academic level of TCM in our country, but also an urgent research topic that need to be worked on. In this paper, the contents and methods of research on the inheritance of prestigious TCM doctors' experiences are discussed. There are three main research issues: the clinical experiences, the academic thoughts, and the ethics of prestigious TCM doctors. And when doing research on the methods, these problems should be paid attention to: study prestigious TCM doctors' clinical experiences, academic thoughts and ethics at the same time; start the research with trying to find a doctor's own character by comparison; understand the origin and development of a doctor's academic thought from the culture background; pay attention to the process of making tacit knowledge explicit; do more researches on the evaluation, promotion and application of prestigious TCM doctors' experiences based on EBM (evidence-based medicine).