Objective To investigate the changes and relationships between the serum endogenously produced interleukin-10(IL-10),nitric oxide synthase(NOS) and inducible nitric oxide synthase(iNOS) in acute myocardial ischemia and reperfusion.Methods Forty-two rats were randomly divided into two groups: myocardial ischemia and reperfusion group(the control group) and methylprednisolone treatment group.The rats in the control group and methylprednisolone treatment group received intravenous injection of placebo and methylprednisolone before ischemia respectively.The plasma IL-10,NOS and iNOS were detected at 0.5 h after ischaemia and 0.5h,2 h after reperfusion,respectively.Result The plasma IL-10,NOS and iNOS gradually increased at 0.5 h after ischaemia and 0.5h,2 h after reperfusion in the two groups.The levels of plasma IL-10 were significantly higher in the methylprednisolone treatment group in different time points than those in the control(all P

Two new neolignans and one new lignan (1-3) were obtained from the roots of Paeonia lactiflora. Their structures were unambiguously elucidated based on extensive spectroscopic analysis, single-crystal X-ray crystallography, and the calculated and experimental electronic circular dichroism (ECD) spectra. Compound 1 was a racemic mixture and successfully resolved into the anticipated enantiomers via chiral-phase HPLC. Compound 3 demonstrated moderate inhibitory activity against human carboxylesterase 2A1 (hCES2A1) with an IC₅₀ value of 7.28 ± 0.94 μmol·^-1.
Chromatography, High Pressure Liquid ; Humans ; Lignans/chemistry* ; Paeonia ; Plant Roots/chemistry* ; Stereoisomerism