According to the speciality of medical occupation and the form of professional ethics education (including its significance,content and method) received by medical personnel at school,this essay is to elaborate the life effect of professional ethics education in medical practice,which plays an important role in the improvement of public efficiency and economic profit,and the development of medical science in a healthy way.

According to the law,signing the operation agreement between patient and hospital before operation is one of the most important parts of legal treatment.It discusses the importance of the effect,error regions in applying and modeling the operation agreement before operation.It discusses the function of operation agreement in protecting both patients' and medical staff's right,reducing medical disputes and strengthening the confidence of patients against diseases from the point of view of ethics.At the same time it analyses how to perfect and regulate the content and procedure of the agreement and have a good relationship between doctors and patients.

0.05).⑵ With the increase of titers of anti-A(B) antibodies of IgG type, both the incidence and severity of HDN elevated (P

Objective To establish the technique of loading trehalose into human platelets including temperature, time and concentration of trehalose loading. Methods Intracellular trehalose concentration versus loading time curves at 37℃ and 16℃, mean plateletee volume(MPV) versus loading time curves, and loading concentration curves were investigated and compared. Then loading time and temperature for high loading efficiency of trehalose into human platelets were ascertained. Intracellular trehalose versus trehalose loading concentration curves were made, and loading efficiency was caculated. Results The trehalose loading efficiency of 4 hours at 37℃ was significantly higher than that at 16℃ (11.6% vs 5.6%). MPV at 16℃ increased 43.2% compared with at 37℃, but had no changes along with the change of loading time and concentration. When loading at 37℃, MPV change was positively related with loading time and concentration, and loading time and loading concentration had a cooperative effect. MPV had no changes in 1～4hours loading when trehalose loading concentration was under 50mM. MPV increased with the increase of loading time and concentration when trehalose loading concentration was above 50mM. When trehalose loading concentration was 45mM, loading efficiency at 37℃ reached 13.86% 4 hours aftr loading. Conclusion The basic parameter of trehalose loading technique of human platelets was loading temperature at 37℃, loading time for 4 hours, and loading concentration of 45mM.

0.01).After incubation with trehalose for 4h,the expression of CD62p increased,and it decreased when platelets activation inhibitor PGE-1 and adenosine were added to the incubation buffer.The expression of PAC-1 showed no difference among the three groups. Conclusion The preprocessing before platelets lyophilization has no significant effects on the function and activity of platelets when the platelets activation inhibitor is added.

Acupuncture Points ; Adult ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Electroacupuncture ; Female ; Humans ; Hyperprolactinemia ; therapy ; Young Adult

0.05).Conclusion Platelets maintain a good and stable quality at-80 ℃ and the-80 ℃ refrigerator may cryopreserve the platelets for a long time.

Objective To estimate the efficacy and safety of cryopreserved platelets in surgical patients.Methods 238 patients in recent years with hemorrhage or high risk of hemorrhage induced by thrombocytopenia or functional platelet defects during or after surgery, who had been transfused with cryopreserved platelets, were included in our study. The following items were recorded: bleeding time before and two hours after transfusion of cryopreserved platelets; the persist time of blood oozing from wound after transfusion; platelet count before and one hour after transfusion; platelet count before and immediately after surgery; and the number of patients with adverse transfusion reactions. The hemostatic efficacy was judged by the bleeding time and platelet count change.Results Bleeding times before and two hours after cryopreserved platelets transfusion were (9?3)min,(4?2)min respectively; blood oozing from wound stopped 0.5～2 hours after transfusion; the platelet level was elevated by(5～42)?10 9/L one hour after transfusion; the postoperative platelet count was decreased by(130?69)?10 9/L compared to preoperative count; four patients had urticaria and five had febrile reaction. The hemostatic efficacy was 95%(226/238) Conclusion Cryopreserved platelets function immediately to bring about the cessation of blood oozing from the wound,and elevation of peripheral blood platelet count.

Objective 1) To establish and optimize a flow cytometry method for detecting CD62p reexpression by preserved platelets.2) Daily determination of the CD62p expression by platelets stored at 22℃ to assess its value in evaluation of preserved platelet quality.Methods 1) Statistic methods including factorial experiment was applied to optimize the major conditions for sample management, and the feasible negative and positive control for FCM analysis of CD62p expression were check out.2) Thirteen donors' platelets including ten PRP and three apheresis concentrations were stored in a flatbed shaker at 22?2℃ for 12 days. The CD62p expression were detected daily. The correlation between CD62p expression and in vivo residual life span of 22℃ stored platelet were analyzed.Results 1) Satisfactory conditions for analysis CD62p anew expression by FCM were obtained. 2) The CD62p anew expression by stored platelets was correlated to the in vivo residual life span of the platelets with the correlation coefficients between 0.91 and 0.99.Conclusion 1) This FCM method is simple, sensitive, stable, and easy to carry. 2) Platelet CD62p expression can be used to monitor the function of stored platelets and has the potential in the study of new platelet preservation technique.

Objective To evaluate the efffects of leukocyte-depleted blood transfusion the protection of cardio pulmonary tissue and the reduction of postoperative bacterial infection.Methods Three hundred and forty-four pediatric patients were allocated to one of 3 groups based on transfusing different components of blood during open heart surgery:control group(n=109),routing blood transfusion group(RBT,n=113),leukocyte depleted blood transfusion group (LDBT,n=122). Blood samples were taken before operation,at 6h,and 24h after operation fir determination of creatininee kinase MB( CK MB),aspartate transaminase(AST),lactate dehydrogenase(LDH)and MDA. At the same time,we monitored changes of TPR and RI,and recorded changes of body temperature and days of utilizing antibiotics after operation.Results After operation serum concentrations of CK MB,AST, LDH and MDA significantly increased in all groups as compared to the values before operation,but the values of those in group LDBT were significantly loert than those in group RBT(P0.05).Conclusions Leukocyte depleted blood transfusion can effectively prevent the patients from cardion pulmonary ischemia-reperfusion injury and reduce postoperative bacterial infection in pediatric open heart surgery.