Objective To develop a method for determining the concentration of ziprasidone in human plasma by using HPLC . Methods The drug concentration of plasma was analyzed in a reverse phase HPLC system C18 column(250 mm × 4 .6 mm ,5 μm);mobile phase consisted of 0 .2% ammonium triethyamine‐methanol(15∶85);the flow rate was1 .1 mL/min;the column temperature was 35°C .The detection wavelength was at 230 nm .Ethyl acetate was used as extracting solvent .Results A good linearity range was 8 .0-300 .0 ng/mL concentration of ziprasidone in plasma .The average recoveries of ziprasidone in low ,middle and high con‐centrations(8 .0 ,80 .0 ,350 .0 ng/mL) over 95% respectively ;the extraction recovery over 90% .The intra‐day and inter‐day varia‐tion(RSD)was less than10% (n=5) .The minimum detectable concentration of ziprasidone was 5 ng/mL .The regression equation was Y=0 .089 9X+0 .021 5 .r=0 .999 7(n=7) .Conclusion The method is fast ,accurate ,,sensitive and simple for clinical monito‐ring of ziprasidone in plasma .

Objective To study influence of psychological intervention on life quality of patients with non-erosive reflux disease(NERD) complicated with depression. Methods Patients with NERD and depression(95 cases) were divided randomly into the observation group(48 cases) and the control group(47 cases).The control group received oral administration of rabeprazole,10 mg,bid.The observation group was given psychological intervention based upon routine medication.Tbe reflux diagnostic questionnaire (RDQ), self-rating depression scale(SDS) and quality of life-index(QL-INDEX) scale were adopted for evaluation before and 8 weeks after treatment. Results The score of SDS was (41.58±6.85) in the observation group,which was lower than that of the control group,(50.42±9.26), after 8 weeks of treatment (P<0.01). The score of QL-INDEX was (8.56±1.38) in the observation group,which was higher than that of the con-trol group,(7.98±1.42), after 8 weeks of treatment (P<0.05). Conclusions Psychological intervention for patients with non-erosive reflux disease (NERD) complicated with depression can ameliorate their de-pression state and improve their life quality.

Objective To observe the expressions of microRNA-126 (miR-126) and vascular endothelial growth factor (VEGF) in gastric carcinoma tissues,and their associations with clinicopathologic features in gastric carcinoma.Methods Fifty cases of gastric carcinoma and matched tumor adjacent tissue specimens were collected from January 2012 to March 2014 in Shangqiu First People's Hospital of He' nan Province.Expression levels of miR-126 and VEGF were examined using reverse transcriptase-polymerase chain reaction.Their correlations and the relationships between them and the clinicopathologic features in gastric carcinoma were analyzed.Results The expression levels of miR-126 in the tumor tissues and in the adjacent tissues were 0.652 ± 0.102 and 0.379 ± 0.069,and the expression levels of VEGF in the two kinds of tissues were 1.523 ± 0.142 and 0.604 ± 0.152,with statistical differences (t =8.374,P =0.001;t =29.508,P =0.000).The expressions of miR-126 and VEGF were significantly positively correlated (r =0.735,P =0.001).The expressions of miR-126 and VEGF were associated with TNM stage (t =22.981,P =0.001;t =18.053,P =0.001),depth of tumor invasion (t =26.792,P =0.001;t =20.234,P =0.001) and tumor node metastasis (t=26.668,P=0.001;t=14.857,P=0.001).Conclusion miR-126 is high expressed in the gastric carcinoma tissue,which may up-regulate the expression of VEGF,and lead to the formation of new blood vessels,then to promote gastric carcinogenesis and the development process.

For comprehensive ability's cultivation of clinical graduate students,this article presents new and feasible methods in placing emphasis on cultivating teaching ability,in order to improve clinical graduate student's teaching ability.It also provides feasible experience and lessons for many other medical schools on graduate cultivating and management.

Objective:To discuss the clinical efficacy of minimally invasive locking plate and the traditional open reduction with internal fixation in the treatment of proximal humerus fractures.Methods:89 cases of patients with proximal humeral fractures were selected and divided into two groups according to different surgical methods.The observation group (45 cases) was given minimally invasive locking plate,while the control group (44 cases) was treated with the traditional open reduction and internal fixation.The operation time,hospitalization time,fracture healing time,intraoperative blood loss,Neer score and Constant-Murley score at 1 month after postoperatiion were compared between two groups.Results:The operation time,hospitalization,fracture healing time of observation group were significantly shorter than those of the control group,and the bleeding amount of observation group was less than that of the control group (P＜0.05).At 1 months after operation,the Constant-Murley scores of observation group were significantly better than those of the control group (P＜0.05).The For Neer scores,excellent rate of observation group (91.1％) were significantly higher than those of the control group (68.1％,P＜0.05).Conclusion:Compared with the traditional open reduction with internal fixation,minimally invasive locking plate was better,safer,faster and more effectively for promoting the recovery of shoulder function of patient with proximal humerus fractures.

The main purpose of this study is to observe and analyze the characteristics and the clinical significance of long-term echocardiogram data in patients after mechanical aortic valve replacement operation. Fifty-five cases were recruited over 5 years after mechanical aortic valve replacement operation, with complete echocardiogram examination profiles. The 55 subjects were divided into predominate aortic stenosis group and predominate aortic regurgitation group, based on preoperative examinations. And another way of division was also carried out that according to the effective orifice area index (EOAI), the subjects were divided into three groups, i. e. , severe prosthesis-patient mismatch (PPM) group, moderate PPM group, and non PPM group. Characteristics of long-term echocardiogram data of the subjects were analyzed. Patients with aortic stenosis showed significantly decreased thickness of intraventricular septum and left ventricular posterior wall about half a year after operation (P < 0.05). Half a year after operation, patients with aortic regurgitation showed decreased left ventricular internal dimension diastole and left ventricular internal dimension systole, and increased ejection fraction (P < 0.05). Differences in mean aortic valve pressure gradient, forward blood flow across the aortic valves, and ejection fraction were not significant among those with severe PPM, moderate PPM, and non PPM groups (P > 0.05). Number of cases of ascending aorta dimension increased as time goes on and pathogenesis of other valve diseases. A conclusion could be drawn that the heart function of patients with aortic valve disease could be improved after mechanical aortic valve replacement operation, but pathologies in other valves and ascending aorta might be increased, and therefore periodical postoperative echocardiogram evaluation with long-term pharmaceutical therapy could be necessary.
Aortic Valve Stenosis ; surgery ; Echocardiography ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation ; Heart Ventricles ; anatomy & histology ; Humans ; Postoperative Period ; Systole ; Treatment Outcome

Objective To study the factors effecting on flow cytometric sperm chromatin structure assay.Methods The SCSA explores the metachromatic properties of acridine orange (AO) and flow cytometry to monitor the susceptibility of sperm chromatin DNA to acid induced denaturation in situ because of the low pH treatment. Results COMP?t, ?t, SD?t value were different upon on the storage methods (①sperm was preserved in 4℃, ②sperm was cryopreservated after dilution ③sperm was cryopreservated). Results show that the second method above made the least artificial injury to sperm DNA. It did not affect the results if the samples were quickly thawed in a 37℃ water bath and detected immediately and the detect current velocity was lower than 300 cell/sec. The intra -CV was 7 28% and the internal CV was 8 92%. Mean and standard deviations of COMP?t were 8 7?11 0% in 511 healthy men. Because data present right skew distribution, the reference range of COMP?t is

Objective To prepare VP1 protein vaccine of Coxsackievirus A16(CA16) and evalu-ate immunngenicity the subunit vaccines of Coxsackievirus (VP1), and to establish foundation for studying CA16 vaccine. Methods CA16 VP1 was amplified by RT-PCR and cloned into pFastBac HT A plasmid, recombinated with Bacmid DNA by transposition reaction and then transfected Sf9 cell, mixed with adjuvant AI(OH)_3. After immunization BALB/c mice, evaluating immune effectiveness after booster injections 2 weeks. Results The expressed protein was analyzed by SDS-PAGE and Western blot, mice immunized with CA16 (VP1) both induced specific IgG antibody and neutralization antibody. The best immunization antigen was 20 μg, IgG antibody was 1: 1600, neutralization antibody was 1:250, typical Th1/Th2 immune response was determined by lymphocyte proliferation assay and cytokine analysis. Conclusion The CA16 VP1 gene was cloned successfully and expressed in Sf9 insect cells, CA16 VP1 protein vaccine induced both humoral and cellular immune response, to lay solid foundation for further study on CA16 vaccine.

Objective To investigate the relationship between hepatitis B virus (HBV) genotype,the mutation in basic core promoter(BCP)region/pre-core(Pre-C)region and the incidence of hepatocellular carcinoma(HCC) in Fusui county of Guangxi Zhuang Autonomous Region (Guangxi),a area with high incidence of HCC. Methods In this case-control study,53 HCC patients and 70 asymptomatic HBV carriers were enrolled. Blood samples were collected from them for serum separation and HBV DNA extraction. The DNA sequences of the S region and BCP/Pre-C region of HBV was determined by direct sequencing following nested-PCR amplification. The relationship between the genotype,gene mutation of HBV and the incidence of HCC was analyzed. Results The mutation rates of the A1762T/G1764A in the BCP region and the T1858C in the Pre-C region of HBV were significantly higher in HCC group than in control group(94.3%vs. 75.7%,P=0.006;50.9%vs. 31.4%,P=0.029). The mutation rate of A1775G was significantly higher in control group (28.6%) than in HCC group (13.2%)(P=0.041). Multiple logistic regression analysis indicated that A1762T/G1764A and T1858C mutations are the risk factors for the development of HCC (OR=5.459,95%CI:1.397- 21.332,P=0.015;OR=3.881,95%CI:1.462-10.305,P=0.006). A1775G is the protective factor in the development of HCC(OR=0.192,95%CI:0.059-0.622,P=0.006). Conclusion The present investigation showed that BCP A1762T/G1764A,A1775G and Pre-C T1858C mutations are correlated with the incidence of HCC in Fusui county of Guangxi.

Objective To study effects of salmon calcitonin and Heng Gu bone healing reagent on vertebral osteoporotic fracture(OPF).Methods From Nov.2007 to Dec.2009,82 cases of vertebral OPF were treated.These cases were randomly divided into treatment group and control group.The treatment group(42 cascs)were treated with salmon calcitonin and Heng Gu bone healing reagent.The control group(40 cases)received salmon calcitonin only.Pain relief of the 2 groups wag compared.Results Before treatment,the 82 patients were scored 6-9 points by visual analogue scales(VAS)and pain scores of the 2 groups were similar (P>0.05).3 days,5 days,8 days and 15 days after treatment,VAS scores of the 2 groups were significantly different(P<0.01).Compared to the control group,the treatment group had pain relief in shorter time and their bone mineral density Was better showed by the 3-month-later review.Conclusion The combination therapy of salmon calcitonin and Heng Gu bone healing reagent in treating vertebral OPF has better effect of pain relief and bone formation,which is a safe and effective method.