ObjectiveTo investigate the related factors of the occurrence of nosocomial infection and nursing management in operating room for general surgery patients,and provide a scientific basis to reduce the incidence of nosocomial infection of the operating room. Methods304 patients with nosocomial infection from August 2009 to August 2010 in our hospital for surgery were set as the control group.151 cases from September 2010 to October 2011 were named as the intervention group.All cases were analyzed retrospectively.The nursing factors which might affect nosocomial infection in operation room were analyzed by univariate and multivariate logistic regression analysis. ResultsBy univariate analysis,eight operation room nursing factors related to nosocomial infection were screened out:the type of incision,operation time,the following surgery or not,into ICU time,preoperative use of antibiotics,visitors of operations or not,invasive operation.5 highly correlated factors were screened out by multi-factor logistic regression analysis:admission to the ICU time,incision type,operation time,preoperative use of antibiotics,then following surgery.The infection rate and prolapse rate of the control group were significantly higher than those of the intervention group. ConclusionsIn nursing work of the operation room,we can take a series of nursing management interventions to reduce the incidence of nosocomial infection of general surgery.