Objective To explore the distrbution characteristics of glutathione S-transferase T1 and M1 (GSTT1 and GSTM1) gene polymorphism of Daur population in Inner Mongolia region, and provide the relevant data for studying the genotype of minority ethnic group of the Inner Mongolia.Methods Genomic DNA was extracted from peripheral blood and the fragments of GSTM1 and GSTT1 was amplified by internal control allele specific PCR in 220 volunteers who had been residents in Inner Mongolia region. At the same time, the genotype of GSTT1 and GSTM1 was resolved by the density of 2.5% agarose gel electrophoresis.Results In the studied population, the frequencies of GSTM1 (-) and GSTT1 (-) were 50.8% and 71.4% respectively. The frequency of the individuals with combination of GSTM1 (-) and GSTT1 (-) was 31.4%.Conclusion The distribution of GSTM1 and GSTT1 displays gene polymorphism in Chinese Daur population, approximate to Mongolian population in the north of China, but there is significant difference between Chinese Daur population and Han people.

Objective To investigate the effects of hyaluronan (HA) and hyaluronidase (HAse) on the formation of hypertrophic scar in rabbit ear after injury. Methods Wound healing models were established on 18 adult rabbit ears, which were randomly divided into the HA treated group (Group A), the HAse treated group (Group B), the PBS treated group (Group C). The process of wound healing was observed grossly and histologically. The variation of the contents of hydroxyproline(HPr) at the stage of wound healing and post healing was measured. The relative height of scars were measured under microscope at the peaks of scar formation. Results ①The mean values of wound healing time of Group A, B and C was 11.7?0.6, 9.8?0.9, 10.8?1.0 days respectively. ②Compared with PBS controls and HAse treated group, the collagon fiber was slender and arranged in normal order in HA treated wound at early stage of wound healing. But there were no difference among them at the stage of scar formation. ③The contents of HPr were statistically lower in HA treated group than in controls at the stage of wound healing, but no difference at the stage of scar formation.The HPr contents in HAse treated group were higher than the others all the way. ④ The mean values of relative thickness of Group A, B and C were 1.55?0.35,1.89?0.39,1.59?0.29 respectively. Conclusion ①Exogenous HA on the wound surface can not affect the scar tissue proliferation;② HAse promotes the proliferation.

Radiotherapy is an effective mean of treatment of gastric cancer. Combination of radiotherapy and chemotherapy in gastric cancer preoperative, postoperative and palliatie treatments can increase the local control rate and the survival rate of gastric cancer patients. With the development of three-dimensional conformal and intensity modulated radiotherapy, the radiotherapy is being more extensively applied in the comprehensive treatment of gastric cancer.

Objective To compare the clinical effectiveness of ozone (O_3) nucleus pulposus ablation only with that of 03 nucleus pulposus ablation combined with intervertebral facet joint injection of compound Betamethasome in treating lumbar disk herniation accompanied with intervertebral facet arthritis. Methods Eighty patients with lumbar disk herniation and intervertebral facet arthritis were equally and randomly divided into two groups. Under CT guidance, O_3 nucleus pulposus ablation was performed in patients of group A(n = 40), while O_3 nucleus pulposus ablation combined with intervertebral facet joint injection of compound Betamethasome (0.5 ～ 1 ml) was carried out in patients of group B (n = 40). Using double blind method the therapeutic effectiveness was evaluated before and 1 week, 3, 6-months after the procedure by an Oswestry Low Back Pain Disability Questionnaire. Results One week after the procedure the effective rate of group A and group B was 65% and 82.5% respectively. Three and six months after the treatment, the effective rate was 75% and 70% respectively for group A, while it was 90% and 92.5% respectively for group B. The difference between two groups was significant (P < 0.05). Conclusion lntradiscal injection of O_3 combined with intervertebral facet joint injection of compound Betamethasome is an effective and safe treatment for lumbar disk herniation accompanied with intervertebral facet arthritis. It is worth popularizing this technique in clinical practice.

BACKGROUND:Ceramic-on-ceramic total hip arthroplasty can be applied to young patients requiring frequent activity and high quality of life.
OBJECTIVE:To explore the short-term efficiency of ceramic-on-ceramic total hip arthroplasty in young and middle-aged patients of femoral neck fractures.
METHODS:Fifty-one young and middle-aged patients under 50 years old with ceramic-on-ceramic total hip arthroplasty were selected to analyze the function of hip joints and the complication after operation.
RESULTS AND CONCLUSION:Al the patients were fol owed up for 6 months to 44 months. At the last fol ow-up, the average Harris score was 92.0±3.4 points with an excellent and good rate of 94.1%. No dislocation was observed and three cases exhibited mild limping. Imaging examination showed that, no prosthesis loosing, broken, sinking or radiolucent line around prosthesis was observed. Ceramic-on-ceramic total hip arthroplasty in young patients with femoral neck fractures can effectively decrease the osteolysis, prosthesis loosing and sinking caused by the debris of wearing, prolong the working life of the prosthesis, and improve the patient’s quality of life.

BACKGROUND:In recent years, the technical parameters about hurdle athletes are mainly obtained through video analysis and DLT algorithm. However, the gait and surface electromyography (sEMG) characteristics during normal walking are little reported. OBJECTIVE: To explore the changes of the gait and lower limb sEMG signals relative to gait period in hurdle athletes. METHODS:Eight male professional hurdlers were selected to perform gait and lower limb sEMG tests on the trail, and the differences in gait and sEMG signals were analyzed by mathematical statistics. RESULTS AND CONCLUSION: The gait parameters of hurdlers showed no significant differences (except step length). In the total gait cycle, along with the gait changing, the right and left side muscles of the same name moved alternately. The median frequency and average power frequency of the tapping leg in the lower limb muscles were greater than those of the swinging leg (except biceps femoris, tibialis anterior and lateral gastrocnemius), but the mean EMG and EMG integral values of the tapping leg were smaller than those of the swinging leg. At the stand phase, the median frequency and average power frequency of the tapping leg in the lower limb muscles were greater than those of the swinging leg (except tibialis anterior), but the mean EMG and EMG integral values of the tapping leg were smaller than those of the swinging leg (except soleus). At the swing phase, the median frequency and average power frequency of the tapping leg in the lower limb muscles were greater than those of the swinging leg (except tibialis anterior and lateral gastrocnemius), but the mean EMG and EMG integral values of the tapping leg were smaller than those of the swinging leg (except soleus). To conclude, there are different degrees of differences in the frequency domain and time domain of the lower limb muscles between tapping and swinging legs. Additionally, the muscle strength of the tapping leg is less than that of the swinging leg.

The main purpose of this study is to observe and analyze the characteristics and the clinical significance of long-term echocardiogram data in patients after mechanical aortic valve replacement operation. Fifty-five cases were recruited over 5 years after mechanical aortic valve replacement operation, with complete echocardiogram examination profiles. The 55 subjects were divided into predominate aortic stenosis group and predominate aortic regurgitation group, based on preoperative examinations. And another way of division was also carried out that according to the effective orifice area index (EOAI), the subjects were divided into three groups, i. e. , severe prosthesis-patient mismatch (PPM) group, moderate PPM group, and non PPM group. Characteristics of long-term echocardiogram data of the subjects were analyzed. Patients with aortic stenosis showed significantly decreased thickness of intraventricular septum and left ventricular posterior wall about half a year after operation (P < 0.05). Half a year after operation, patients with aortic regurgitation showed decreased left ventricular internal dimension diastole and left ventricular internal dimension systole, and increased ejection fraction (P < 0.05). Differences in mean aortic valve pressure gradient, forward blood flow across the aortic valves, and ejection fraction were not significant among those with severe PPM, moderate PPM, and non PPM groups (P > 0.05). Number of cases of ascending aorta dimension increased as time goes on and pathogenesis of other valve diseases. A conclusion could be drawn that the heart function of patients with aortic valve disease could be improved after mechanical aortic valve replacement operation, but pathologies in other valves and ascending aorta might be increased, and therefore periodical postoperative echocardiogram evaluation with long-term pharmaceutical therapy could be necessary.
Aortic Valve Stenosis ; surgery ; Echocardiography ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation ; Heart Ventricles ; anatomy & histology ; Humans ; Postoperative Period ; Systole ; Treatment Outcome

Objective: To observe the effects of vitamin C(VC)on the electrophysiological property of myocardial cells of the rats fed with low selenium and high cadmium fodder. Method: Eight groups of rats were fed separately with: normal (2 groups), high Cd, high Cd+high VC, low Se+high Cd, low Se+high Cd+high VC, high Se+high Cd and high Se+high Cd+high VC fodder for 14 w. With intracellular microelectrode technique, the electrophysiological changes of rats were observed. Results:Compared with the control, there were no changes in high Cd+high VC group and in high Se+high Cd+high VC group. However,in high Cd group, RP and APA decreased, APD50 and APD90 prolonged; in low Se+high Cd group ,RP and APA decreased, APD50 and APD90 prolonged significantly; in low Se+high Cd+high VC group and high Se+high Cd group, though RP still decreased, no change had been detected on APA and APD. Conclusion: Se deficiency and simultaneous Cd overabundance may change the electrophysiological properties of myocardial cells of the rats significantly. However, VC may protect myocardial cells against the injury effectively.

This study examined the effect of P85 (a pluronic block copolymer) and microbubble (MB) ultrasound contrast agents under ultrasound irradiation on gene transfection and expression. The pEGFP plasmids that can encode enhanced green fluorescent protein (pEGFP) served as a report gene and were mixed with different concentrations of MB/0.05% (w/v) P85. Then the plasmids were transfected into human hepatoma G2 (HepG2) cells. The HepG2 cells treated with MB/P85 or without treatment were exposed to ultrasound (US parameters: 1 MHz, 1.0 W/cm(2), 20 s, 20% duty cycle). Twenty-four hours later, the transfection efficiency was assessed by fluorescence microscopy and fluorescence activated cell sorting (FACS) analysis. The cell viability was evaluated by Trypan blue exclusion test. The results showed that the gene transfection efficiency in HepG2 cells under ultrasound irradiation was significantly higher than that without ultrasound irradiation. HepG2 cells in the MB or P85 group in the absence of ultrasound expressed less amount of green fluorescent protein. The expression efficiency reached (22.14±3.06)% and the survival rate was as high as (55.73±3.32)% in the 30% MB plus P85 group. It was concluded that MB and P85 in the presence of ultrasound can enhance gene transfection and expression.