OBJECTIVE:To explore short-term efficacy and safety of early use of high-dose,medium-dose and low-dose ami-no acid in premature babies. METHODS:99 premature babies were selected and randomly divided into high-dose group,medi-um-dose group and low-dose group,with 33 cases in each group. 3 groups were given Amino acid injection,ivgtt,within 24 h af-ter birth,high-dose group was given 3 g/(kg·d),medium-dose group 2 g/(kg·d)and low-dose group 1 g/(kg·d);those dose in-creased by 0.5 g/(kg·d)day by day;predicted peak values of them were 3.5,3.5 and 3 g/(kg·d),respectively. Treatment courses of 3 groups lasted for 28 d. Health indexes,renal function indexes and blood indexes were observed in 3 groups. The occurrence of complications and ADR were recorded in 3 groups. RESULTS:2 cases withdrew from high-dose and low-dose groups. Hospital-ization stay,the time of body weight increasing to 2 500 g and the rate of body weight decreasing in high-dose group were signifi-cantly lower or shorter than in medium-dose and low-dose groups;the medium-dose group was significantly lower or shorter than the low-dose group,with statistical significance (P<0.05);the levels of creatinine,residual alkali,serum total bilirubin in high-dose group were significantly higher than medium-dose and low-dose groups,and the medium-dose group was significantly higher than the low-dose group,with statistical significance(P<0.05). The number of complications cases in high-dose group(11 cases)were significantly lower than in medium-dose group(20 cases)and low-dose group(26 cases),with statistical significance (P<0.05). No obvious ADR was found in 3 groups. CONCLUSIONS:High-dose of amino acid intravenous nutrition support in early stage can promote the recovery of nutrition state and healthy constitution in premature babies with good tolerance and safety.

OBJECTIVE:To prepare the curcumin solid lipid nanoparticles. METHODS:Microemulsion method was adopted to prepare curcumin solid lipid nanoparticles (SLN) and pseudo-ternary phase diagrams was conducted to optimize the preferable three-phase microemulsion factors. The optimal conditions were determined,and then hot microemulsion was dispersed in cold wa-ter to prepare SLN. After the preliminary screening of the factors (emulsifiers,lipid materials,amount of lipids,drug-to-lipid ra-tio,cold water phase temperature and the holding temperature of microemulsion)by single-factor test,with the index of encapsula-tion efficiency,the orthogonal test was conducted to optimize the formulation,followed by the verification test. RESULTS:The best microemulsion was produced at 65 ℃ with three phases consisting of stearic acid(oil phase),polysorbate 80(emulsifier)and ethanol (co-emulsifier) and the Km ratio of 1∶4. The optimal conditions for SLN were as follows as curcumin dosage of 50 mg, stearic acid of 0.5 g,cold water phase temperature of 2 ℃,holding temperature of microemulsion of 65 ℃. The encapsulation effi-ciency was 87.73% for solid lipid nanoparticles prepared,drug-loading rate was 7.72%,particle size was(156.9±2.2)nm,poly-dispersity coefficient was 0.480,average Zeta potential was -24.8 mV(RSD<2%,n=3). CONCLUSIONS:The microemulsion method to prepare SLN is simple and feasible.

Objective To investigate the effects of demethylating agent 5-Aza-2'-deoxycytidine (5-Aza-CdR) on the biological behaviors and the expression of RASSF1A of lung carcinoma cells H460. Methods Lung carcinoma cell line H460 were treated with 5-Aza-CdR. Cell proliferation was determined by MTT assay and colony forming test. The methylation status of RASSF1A gene was detected by PCR. The expression of RASSF1A protein was measured by Western blotting, and the cell cycle was analyzed by flow cytometry. Results With 5-Aza-CdR treatment, the proliferation speed of H460 lung carcinoma cells was slowed down and the colony formation rate of H460 cells was decreased significantly compared with control group (38.5 %, 27.5 % and 60.5 % in 5-Aza-CdR 5 and 10 μmol/L groups and control group, respectively, P <0.05). The methylation degree in the promoter of RASSF1A gene was decreased and the expression of RASSF1A protein was detected after 5-Aza-CdR treatment. 5-Aza-CdR induced G1 phase arrest of the H460 cells. Conclusion The hypermethylation of CpG island in the promoter of RASSF1A gene results in the loss of RASSF1A protein expression in human Lung carcinoma cell line. The demethylating agent 5-Aza-CdR could restore RASSF1A gene expression.These findings provide theoretic evidence for clinical treatment of human lung carcinoma with demethylation agent 5-Aza-CdR.

Objective To investigate the effect of experimental benzolism on marrow eryghrocyte micromucleus.Methods Fifty male KunMingmice(KM)were randomly divided into 5 groups:one control group and four experimental groups with 10 mice in each group.The mice were injected subcutaneously with sodium chloride in control group and benzene in experimental groups on every Monday,Wednesday and Friday for four weeks.Four experimental groups were injected with a dosage of 0.5 ml/kg(group B1),1.0 nd/kg(group B2),1.5 ml/kg(group B3) and 2.0 ml/ks(group B4),respectively.Before and after injection of sodium chloride or benzene weekly.weight was measured,and the alteration of erythrocyte micronucleus in peripheral blood and marrow were detected.Results ① Compared to the control group,several indices were partly changed in B1 and B2 groups,while all indices changed significantly in group B3 and B4 groups four weeks after injection(P<0.01).②The erythrocyte micronucleus rate of experimental mice in peripheral blood and marrow showed ascending tendency with the extension of benzene injection phase,peak emerged in the third week and decreased in the fourth week.③In the 4th week,compared to the control group,the alteration of erythrocyte micronucleus in marrow in all experimental groups were significantly increaged(P<0.05).Conclusions Benzolism may induce the increasing of micronucleus rate of mature erythrocyte in peripheral blood and erythrocyte in marrow.which can be used to detect status of being restrained in marrow erythrocyte series.

There are many problems about multiple breast cancer (MBC),such as whether it results from intramammary spread of a single tumor, or from two or more separate neoplastic events? Whether MBC has inferior outcome compared with unicentric lesions? Whether MBC is suit for conservative surgery and the sentinel lymph node biopsy, and so on. Thus: We reviewed the prospect for diagnosis and therapy of MBC.

Objective To study the mRNA and protein expressions of the neurofilament in the region of acute spinal cord injury (SCI) of rats after olfactory ensheating cells (OECs) transplantation combined with methyprednisolone administration and investigate the molecular mechanisms of OECs transplantation combined with methyprednisolone administration in promoting the recovery of the spinal cord.Methods Acute spinal cord injury was established in SD rats ( T10 ) by using NYU instrument. The rats were randomly divided into control group, SCI group, DF12 group, OECs transplantation group,methyprednisolone administration group and OECs + methyprcdnisolone group. The mRNA and protein expressions of the neurofilament in the SCI regions of rats in different groups at different time points were detected by using immunohistochemistry, RT-PCR and Western blotting. Results A significant increase of mRNA and protein expressions of the neurofilament could be found in the other five groups compared with the control group at days 7, 14 and 28 after SCI. The mRNA and protein expressions of the neurofilament in the injury region of the OECs group, the methyprednisolone group and the OECs + MP group were more significantly increased than that of the SCI group and the DF12 group. The expression of the neurofilament in the injury region of the OECs + MP group was more significantly increased than that of the OECs group and the MP group ( P ＜ 0. 05). Conclusions OECs transplantation or methyprednisolone administration can induce the mRNA and protein expressions of the neurofilament. Meanwhile, OECs transplantation combined with methyprednisolone administration can significantly increase the mRNA and protein expressions of the neurofilament, as may be one of mechanisms promoting spinal cord repair.

The authors adopted an auto control experimental research on 18 male adult dogs with 36 sides of femoral heads and necks which were divided into two groups. They were separately made models of old fracture of femoral neck and avascular necrosis of famoral head. Two wecks later, The free fibula with microvascular an astomosis was tranplanted. All experimental dogs were examined with X-ray, pathohitology, electron microscopy, 99~mTc-MDP scanning and tetracycling labeling after operation.The experiment showed that the free fibular graft with mierovascular anastomosis could provided a new system of vascula supply to the injured femoral head. The hard free fibula and the metallic internal fixation were inserted in femoral head and neck. After the Operation, in the fracture area the fibula formed a bone-bridge which not only diminished the stress force but also prevented rotation displacement of the bone fracture The periosteum of the transplanted fibula also took part in the repair course of avascular nerosis of femoral head.

Objective To study the effect of recombinant human erythropoietin (rHuEPO) on in aged patients undergoing maintenance hemodialysis. Methods Blood pressure and its mechanism 52 elderly patients undergoing maintenance hemodialysis divided into two groups, HBP group (23 cases with hypertension) and NBP group(29 cases without hypertension). The plasma vasoactive substances were measured by radioimmunological methods. Fifteen cases in NBP group as EPO group were given a bolus of venous injection of rHuEPO (3 000 IU), others in NBP group (14 cases) were injected 0.9% saline as control group. The plasma vasoactive substances were assayed before and 1, 3, 6, 9, 12, 18, 24, 30, 44 hours after therapy with or without rHuEPO. Results (1) The plasma dopamine (DA) in HBP group were significantly higher than that of NBP group. (2) Endothelin levels increased transiently but DA levels increased steadily in EPO group. Conclusions The study suggests that the effect of rHuEPO induced on hypertension in the elderly patients undergoing maintenance hemodialysis is associated with the increasing plasma DA.

Objective To explore the indications of physiotherapy and operative treatment for gluteal muscles contracture, and discuss the principles and necessity of classification in order to facilitate clinical management. Methods The standards of classification of gluteal muscles contracture were based on the signs and pathological examination, It consisted of three stages upon our standards. 210 patients could be divided into stage Ⅰof 23 cases, stage Ⅱand Ⅲ of 187 cases. For the patients of stageⅠ, physiotherapy was performed; However, all cases of stageⅡand Ⅲ, operative treatment was adopted by small superomedial incision above the greater trochanter. Furthermore, in the cases of stage Ⅱ, tight fibrotic gluteal muscles and thick contracted fascia lata should be released; For stage Ⅲ lesion, the release should be extended to the capsule of hip, and incision of the posterior capsule was necessary. Results In the group of stageⅠ, The symptoms and signs were relieved by maneuver , the rate of excellent and good results was 39%; while in the group of stageⅡand Ⅲ, it was 97% by operative treatment. Conclusion The staging system of gluteal muscles contracture could demonstrate exactly the severity degree of the disease, and is useful in choosing therapeutic method. Non-operatiive method can obtain satisfactory results in about 1/3 cases of stage Ⅰof gluteal muscles contracture; operative method is indicated for the patients of stage Ⅱand Ⅲ.

Objective To discuss the therapeutic effect of fracture operation on multiple bone injuries. Methods The patients with fractures were treated with 3 different methods, ie, emergency, selective and conservative treatments. Early complications such as acute respiratory distress syndrome (ARDS),fat embolism syndrome (FES),duration in insive care unit and mortality rate were compared with the long-term complications of fractures so as to evaluate the curative effect of 3 different methods on multiple bone injuries. Results Among 91 cases (ISS≥30), one died and 2 resulted in FES. Mortality rate, short-term and long-term complications and mean ICU duration were reduced significantly in the emergency treatmentgroup compared with the other two groups ( P