Septic encephalopathy( SE) is defined as diffuse brain dysfunction that related to systemic inflammatory response and without clinical or laboratory evidence of central nervous system infection.SE is one of the most common complications of patients with severe sepsis.Its pathogenesis is very complex and is not yet clear.The clinical manifestations of SE varied and have no specificity, mainly for changes of con-sciousness and mental status.The performance of electroencephalography is more sensitive,and is associated with prognosis.Somatosensory evoked potentials could display the damage of cortical or subcortical path-ways.Brain MRI has a more accurate assessment of the nature and the extent of brain damage.SE is a diag-nosis of exclusion.Before the diagnosis of SE,we need to exclude other encephalitis and encephalopathy.The morbidity and mortality of SE are high.It requires close attention,early detection and timely treatment.

Objective To explore the coping styles and family environment in patients with depression.Methods 90 patients with depression were evaluated with CSQ and FES-CV.Results There were significant differences in the family environment between depression group and normal control group(P＜0.01).The factor scores of seeking-help and solving question in depression group positively correlated with the factor scores of family intimacy and success(r=0.29,0.32,0.27,0.31,P＜0.05).The factor score of self-accusation in depression group negatively correlated with the factor score of affection expression(r=-0.27,P＜0.05).Conclusion The coping styles are associated to family environment in patients with depression.

Objective To verify the flow field performance of the air dynamic wind tunnel. Methods The performance of the flow field was evaluated by testing the flow speed of sample-point on the sample-transect of the tunnel. Results Among the flow range of the tunnel, the relative deviations of the speeds of each sample-point on the sample-transect were less than 10%. When the flow rate was set, the relative deviations of the sample-transect average speed in different test period were less than 10%. The ratio of average wind velocity of upper reach to down reach of the tunnel was between 0.92 and 1.03. Conclusion The flow field of the air dynamic wind tunnel is even, stable and coincident.

Four fresh corpses of infant are used in this research. Three-dimensional microvasculatures of the sinoatrial node and atrioventricular node were studied by means of the observation of vascular casts with SEM. The results showed that the vascular bed of the sinoatrial node was consisted of the microvascular networks. It was oval in shape. The central artery penetrated this node, through its longitudinal axis and divided progressively into arterioles and precapillary arterioles and finally, they branched into capillary networks at the superficial part of the node. The postcapillary venules were characterized by draining blood in accordance with area. The microvascular construction of atrioventricular node was an oblate microvascular network in shape. There was a layer of delicate capillary networks at its superficial part. In the depth of the capillary networks, the venous plexus which was thick and sinusoidal in shape was observed through the meshes. The artery of atrioventricular node entered the node from one side of it. In the node, the artery ramified by degrees to periphery into precapillary arterioles, which penetrated venous plexus and connected with capillary networks in the superficial part of the node. There were evidently narrow rings, whick were impression of the muscle sphincters at the origin of thep ostcapillary venules.

The clinical teaching of ICU is a new challenge.According to the problem encountered in the practice we should improve and better the teaching methods such as asking students to pay more attention to the combination of substructural subjects and critical medicine and having a clinical macrocosm thinking,good responsibility,and the "intensive" concept during their work so that student can learn more initiatively and get more as well.

Objective To evaluate the clinical value of the chemiluminescence immunoassay particles (CMIA)in the diagnosis of syphilis.Methods The serum specimens from 150 cases of syphilis in our hospital were selected as the observation group and con-temporaneous 150 serum samples from non-syphilis healthy people were selected as the control group.The toluidine red unheated serum test (TRUST),treponema pallidum particle agglutination test (TPPA)and CMIA were adopted to conduct the detection. The sensitivity,accuracy and specificity of the three kinds of method were calculated and their differences were compared and ana-lyzed.Results The sensitivity of TRUST and TPPA and CMIA was 65.3%,97.7% and 99.3% respectively,the specificity was 74.7%,97.3% and 100.0% respectively,the accuracy was 70.0%,97.0% and 99.7% respectively,the difference among three kinds of methods had statistical significance (P <0.05).The sensitivity,specificity and accuracy of TRUST were lower than those of CMIA and TPPA (P <0.017),there was no statistically significant difference between CMIA and TPPA (P >0.017).Conclu-sion CMIA is equivalent to TPPA in the sensitivity,specificity and accuracy,is superior to TRUST,and has the advantages of sim-ple operation,objective results and good repeatability.

Objective To evaluate the CT value in diagnosis and differential diagnosis of splenic tumors.Methods 52 cases of splenic tumors were examined by plain and contrast-enhanced CT scan.27 cases of splenic tumors were confirmed by operation and pathology,25 cases were definited by clinical history and having typical imaging signs.Results There were 18 benign and 34 malignant tumors.Most of benign splenic tumors appeared as single or multiple low dense nodus with different size,one hemangioma was isodense.Calcifications could be showed in splenic hemangioma and lymphangioma.Homogeneous and marked enhancement occurred in hemangiomas at delayed-phase,no or slightly enhanced in the septum of splenic lymphangiomas,and the cysts had no enhanced.The malignant tumors of spleen were bigger in size mostly with unhomogeneous density,and unhomogeneous enhancement could be seen at contrast-enhanced CT scan.Uneven enlargement of spleen was commonly seen in patients of splenic lymphoma and the lesions were multiple in a most of cases,and had not enhancement obviously.Conclusion Splenic tumors can be diagnosed correctly in most patients based on the features of plain and contrast-enhanced CT in combination with clinical history and the other examinations.

Hepatitis C virus (HCV), one of the major pathogens of viral hepatitis, causes significant hazards in humans. Interferon treatment in combination with ribavirin is used as the first line clinical treatment for HCV infection. However, good response to this treatment has only been observed in few patients and repeated recurrence has also been reported frequently. Therefore, new antiviral agents and therapies are in urgent demand. Here, we report a newly constructed Escherichia coli RNase P based M1GS ribozyme that can specifically and efficiently target the core gene of HCV. The guide sequence (GS) of this M1IGS was designed according to the sequence of the core coding region of HCV genome. The GS was then covalently linked to the 3' terminus of M1 RNA, the catalytic subunit of RNase P from Escherichia coli. The specification of this sequence-specific ribozyme, M1GS, was then examined using an in vitro cleavage assay. The cytotoxicity and its activity in inhibition of HCV gene expression and viral proliferation were further studied in vivo. Our results show that the reconstructed M1GS ribozyme displayed obvious catalytic activity in cleaving target mRNAs fragment in vitro. Notable reduction in the expression of HCV core protein and a 1 000-fold reduction in viral growth were also observed in cultured HCV infected Huh7.5.1 cells expressing the functional M1GS ribozyme. This study demonstrated a direct evidence for the antiviral activity of the customized M1GS-HCV/C141 ribozyme, and thus provided a promising new strategy for clinical treatment of HCV infection.
Antiviral Agents ; pharmacology ; Escherichia coli ; genetics ; Genetic Engineering ; Hepacivirus ; genetics ; physiology ; RNA, Catalytic ; genetics ; pharmacology ; RNA, Guide ; genetics ; Ribonuclease P ; genetics ; Viral Core Proteins ; genetics

Objective: To observe the changes of blood coagulation factors and inflammatory factors and the regulatin effect of Omegaven.Methods: Thirty ICU patients were randomly allocated into two groups: Omegaven group and control group.Serum concentrations of NF-κB、PGF1α、Eselectin、sICAM-1、TAT、D-dimer、TMD、PF1+2 were measured before and after parenteral nutrition.Results: Patients in control group had significantly higher NF-κB、Eselectin、sICAM-1、TAT、D-dimer、TMD、PF1+2 concentration,lower PGF1α concentration.Patients treated with Omegaven had significantly lower NF-κB、Eselectin、sICAM-1、TAT、D-dimer、TMD、PF1+2 concentration,higher PGF1α concentration.Conclusion: Omegaven can suppress inflammatory reaction and improve coagulopathy.

目的调查特勤疗养人员社会适应的特点。方法以症状自评量表（SCL-90）中与社会适应有关的3个因子（人际关系、敌对、偏执）对558名特勤疗养人员进行测评。结果特勤疗养人员3个因子有阳性症状的百分比为人际关系7.2%、敌对9.3%、偏执6.5%；特勤疗养员的得分明显低于军人常模和地方常模（P<0.01）；海勤疗养员在人际关系、敌对因子上明显高于空勤疗养员（P<0.01），偏执因子无显著性差异（P>0.05）。结论特勤疗养员的社会适应状况良好。