BACKGROUND:Recently, bone tissue engineering is highlighted from the nature source. OBJECTIVE:To explore the preparation of a cuttlebone-transformed hydroxyapatite scaffold as the novel bone substitution. METHODS: As raw materials, the cuttlebone appeared to have a hydrothermal reaction with diammonium phosphate under specific conditions, and the hydrothermal products were characterized respectively by X-ray diffraction, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy and scanning electron microscopy. RESULTS AND CONCLUSION:The results showed that the hydrothermal products were hydroxyapatite. The cuttlebone-transformed hydroxyapatite possessed good three-dimensional structure. Cytocompatibility of the cuttlebone-transformed hydroxyapatite with MG63 cels culturedin vitro were observed and we found the cuttlebone-transformed hydroxyapatite had no obvious effects on cellgrowth and no toxicity, which can be used as new bone tissue engineering scaffolds.

Benign symmetric lipomatosis is a rare disease and may appear as a huge tumor in the neck. Four benign symmetric lipomatosis associated with gigantic painless mass or neck motion limitation were reported. Operative technique of one-time radical resection or stage resection was used to remove these tumors. One patient had a postoperative complication of incision effusion and infection, and three patients had no significant complication. At more than one-year follow-up, the motion and appearance of a patient's neck returned to normal, and no recurrence was observed. The etiology, clinical manifestations, diagnosis, and treatment of the disease were discussed.
Humans ; Lipomatosis, Multiple Symmetrical ; Neck ; Recurrence

Early diagnosis of malignant lymphoma is often difficult since the clinical manifestation of the lymphoma occurred in the maxillofacial region is very similar to that of the squamous cell carcinoma.When the pathological diagnosis is not clear,the surgeon is easy to misdiagnose and lead to mistreatment.A patient visited the Affiliated Haikou Hospital of Xiangya School of Medicine,Central South Universily for gingival mass with an ipsilateral submaxillary enlargement.The clinical manifestation and preoperative MRI are very prone to squamous cell carcinoma with metastasis,so we did not take a preoperative pathological examination.The gingival mass was surgical removed firstly,but frozen pathological result showed that it was malignant small round cell tumor.Since the patient was diagnosed as high degree malignant of small round cell tumor and the submandibular region have been significantly metastasized,so we carried out the combined radical dissection of gingival,mandible and neck surgery.The postoperative pathological report was malignant lymphoma,suggesting that the patient was a case of misdiagnosis and mistreatment.This article draws lessons from misdiagnosis and provided experience for seeking improvement measures.