BACKGROUND: Some researches report that diffusion-weighted imaging (DWI) can appear abnormal signal in minutes after cerebral ischemia.OBJECTIVE: To investigate the manifestation and regularity of diffusion coefficient in middle cerebral artery occlusion (MCAO) of rats after cerebral ischemia.DESIGN: Randomized controlled study.SETTING: Neurological Department of Clinical College of ChongqingMedical University.MATERIALS: The experiment was conducted at the Radiological Department of the First Affiliated Hospital of Chongqing Medical University from April to July 2004. Totally 18 adult Wistar rats with clean grade were randomly divided into three groups: control group, sham operation group and cerebral ischemia group with 6 rats in each group.METHODS: The origin of right middle cerebral artery was blocked with filament into right internal carotid. In the sham-operated group, the filament was just inserted into the common carotid artery not into the origin of middle cerebral artery, rats in normal control group were not treated with anything after anesthesia. MRI experiments were performed on a 1.5 T GE signa Highspeed NMR spectrometer to obtain DWI and ADC values at the time-point of 7, 15, 30, 60 minutes, 3,6, 12, 24 hours, 3, 7, 15 days, 1 and 2 months after operation.MAIN OUTCOME MEASURES: Changes of ADCs in infarct hemisphere and contralateral hemisphere.RESULTS: There was no significant change in MRI T1-and T2-weighted imaging in rats at 7 minutes post-infarction, but abnormal high intensity was emerged during the period of 1 hour to 3 hours, and enlarged over time. Finally, the infarction area located the right basal ganglia and the frontal-parietal lobes at 12 hours and increased less significantly later.Focal abnormal high signal was noted in DWI 7 minutes after cerebral ischemic insult, and the ADC values was decreased at the same time,reduced gradually after significant recover at 30 minutes, dropped to the lowest level at 12 hours and again increased at 24 hours, since then all the ADC values were remained high throughout follow-up, which increased by 5 times at 2 months than that at 12 hours. Correspondingly,rADC values showed the contrary pattern of evolution.CONCLUSION: It is reflected that ADC is highly sensitive not only to onset of cerebral ischemia but also to chronic cerebral ischemia.

Cytochrome P450 is one of the microsomal mixed function oxidase,and plays an important role in the metabolism of exogenous and endogenous material.Lots of domestic and overseas relative literatures were red,the article summarized the research progress of traditional Chinese medicine affecting the metabolism of CYP in recent years.Eight main drug-metabolizing enzymes were respectively introduced,including CYP1A1,CYP1A2,CYP2A6,CYP2C,CYP2D6,CYP2E1,CYP3A4 and CYP3A4.It is important to research CYP450 for drug screening,individual administration.

Medical charts of tuberculotic patients with secondary autoimmune hemolytic anemia (AIHA) admitted to Peking Union Medical College Hospital (PUMCH)from 2006 to 2015 were retrospectively reviewed.Four cases with complete information of laboratory tests,imaging findings and bone marrow results were included.Therapy regimens and outcomes of survival were followed by phone call if needed.Ages at diagnosis of AIHA were 58-80 years old,hemoglobin levels were 31-73 g/L,Coomb tests were negative in all cascs.Thcrapy of cortical steroid did not work,while anti-TB therapy (including isoniazid,rifampicin,ethambutol) significantly improved hemoglobin to normal level in three cases during six to nine months.One case died within one week,for progressive TB to central neurological system.As one of rarc complications of TB,AIHA may be fatal.Early diagnosis and appropriately anti-TB therapy is helpful for good outcomes.

ObjectiveTo analyse the changes of electrolytes and electrolyte disturbances within 72 h in asphyxia neonates. MethodsTwenty-three severe-asphyxia neonates (asphyxia group) and 27 mildasphyxia neonates(control group) were selected.Venous blood samples of 2 ml were drawn at 0-24 h, 25-48h and 49-72 h respectively. The changes of electrolytes(such as plasma sodium, potassium, chlorine,calcium and magnesium) and the incidence of hyponatremia,hypokalemia,hypocalcaemia and hypomagnesemia were compared between two groups. ResultsThe levels of plasma sodium, calcium at 0-24 h, 25-48 h and 49-72 h in asphyxia group were lower than those in control group (P ＜ 0.05). The levels of plasma magnesium at 25-48 h and 49-72 h in asphyxia group were lower than those in control group (P ＜ 0.05 ), but there was no significant difference at 0-24 h (P ＞ 0.05 ). The levels of plasma potassium at 49-72 h in asphyxia group was lower than that in control group(P＜ 0.05). There were no obvious changes in plasma chlorine, phosphorus in two groups (P＞0.05). Hyponatremia, hypokalemia, hypocalcaemia was happened within 72 h in asphyxia neonates especially at 25-48 h, but hypomagnesemia was more at 25-48 h and 49-72 h. ConclusionElectrolyte disturbances can appear not only in 24 h, but also in different periods after asphyxia, therefore, it is necessary to maintain homeostasis by taking corresponding measures according to the changes of blood gas and the changes of electrolytes.

Objective To study the correlation between multi-slice CT perfusion imaging and angiogenesis in hepatocellular carcinoma(HCC) induced by hypoxia inducible factor-1?(HIF-1?).Methods Multi-slice CT perfusion data were obtained in 34 cases of HCC and 11 cases of hepatic hemangioma identified by operation and pathology.Positive expression of HIF-1? and vascular endothelial growth factor(VEGF) protein of the lesions in all cases and surrounding normal hepatic tissue were investigated with SP immunohistochemistry.Microvessel density(MVD) was determined by anti-CD34 immunostaining.The correlation between multi-slice CT perfusion data and positive expression of HIF-1?,VEGF and CD34 was investigated simultaneously.Results Hepatic arterial perfusion(HAP) and hepatic arterial perfusion index(HAI) in HCC were higher than that of hepatic hemangioma and surrounding normal hepatic tissue(P

Objective To study the situation of pathogenic bacteria of neonate infection in hospital,and to help shepherd clinical doctors use antibiotic rationally.Methods MIC and K-B methods were used to test 1098 specimens.Results 142 pathogenic bacterias were isolated.According to our materials S.aureus was the main pathogenic bacteria and the next were S.haemolyticus,S.epidermidis,E.coli,E.cloacae and K.pneumoniae respectively.The MRSA-positive rate in 28 strains of S.aureus was 54%;ESBLs-positive in E.coli was 21.4%;ESBLs-positive in K.pneumoniae was 20.0%.Now the drugs of sensitivity for Grgm positive were vancomycin(0%),clindamycin(8.4%) and cipnofloxacin(12.0%).The drugs of sensitivity for Grgm negtive rods were imipenem(3.6%),cipnofloxacin(7.1%) and amikacin(12.5%).Conclusion It is instructive to use antibiotic rationally for controlling neonate infection in hospital.

The effect of rapepollen on the level of serum cholesterol, triglyceride and fatty acids were investigated experimentally in rats. Results revealed that the rapepollen had potencies to lower cholesterol and triglyceride and alter the composition of serum fatty acids such as decreasing oleic acid and increasing archidonic acid in the rapepollen fed group as compared with control.

Objective:To investigate the clinical efficacy and safety of Reduning injections in the treatment of infantile rotavirus enteritis. Methods:Totally 102 cases of infantile rotavirus enteritis in our hospital from June 2012 to June 2013 were randomly divided into two groups, the control group had 50 patients, and the treatment group had 52 cases. Both groups were given conventional symp-tomatic treatment, the control group was given 10 mg·kg-1 ribavirin injections in 100ml glucose injections for intravenous infusion, qd, and the treatment group was given 0. 5 ml·kg-1 Reduning injections in 100ml glucose injections for intravenous infusion, qd. Ev-ery 3-day was a course of treatment. The clinical curative effect of the two groups was observed, and the clinical symptoms, hospitaliza-tion time and adverse reactions were also studied. Results:After the treatment, the total effective rate in the control group was 70. 0%, while that in the treatment group was 92. 3%, and there was significant difference between the two groups (P<0. 05). The clinical symptom disappearance time and hospitalization time in the treatment group were significantly lower than those in the control group with statistically significant difference (P<0. 05). During the treatment, the control group had 5 cases of leucopenia and 2 cases of skin rash, and the patients all recovered after withdrawal of the drug, while the treatment group showed no obvious adverse reaction. Con-clusion:The curative effect of Reduning injections in the treatment of infantile rotavirus enteritis is promising, which can effectively improve the clinical symptoms, shorten the course of disease and reduce adverse reactions with the value of clinical application.

AIM:To investigate the effect of small incision non-phacoemulsification cataract surgery on corneal endothelial cell.METHODS:Totally 140 cataract patients (152 eyes) treated in our hospital from August 2013 to March 2016 were selected.All of the patients underwent small incision non-phacoemulsification cataract surgery.Before surgery and at 1wk, 1mo and 3mo after surgery, we measured the corneal endothelium of upper cornea, the central cornea and the lower part of cornea.The proportion and density of hexagonal cells, loss rate and cell area in corneal endothelial cells were calculated and compared.RESULTS:Postoperatively 36 eyes had posterior elastic layer folds, aqueous flare, which disappeared after treatment.After surgery, corneal incision was found to have mild corneal edema.No other complications were found.A total of 128 patients (137 eyes) were followed up for a total of up to 3mo, with a loss of follow-up in 12 patients (15 eyes).After surgery, corneal endothelial cell density of the upper part of the cornea, the central cornea and the lower part of cornea, was statistically significantly different with each other (F=3.526, P=0.031), the difference in different time points was statistically significant (F=4.111, P=0.018).The postoperative corneal endothelial cell loss rate was statistically significant different between the three groups (F=3.526, P=0.031), at different time pionts (F=4.111, P=0.018).There was statistical significance of percentage of hexagonal cells between the three groups (F=4.348, P=0.014), the difference was significantly different between different time points (F=4.964, P=0.008).There was statistical significance of corneal endothelial cell area between the three groups (F=3.180, P=0.044), the difference was significantly different between the time points (F=3.511, P=0.032).CONCLUSION:After small incision non-phacoemulsification cataract surgery, corneal endothelial cell density decreased, hexagonal cell proportion decreased, corneal cell area expanded.

This article mainly summarized the safety and effectiveness of SonoVue (LUMASON) in pediatric intravenous application for contrast-enhanced ultrasound examination.