Objective To explore the mediation effect of self-esteem on the relationship between perfectionism and depression in college students.Methods Totally 292 college students completed Frost Multidimensional Perfectionism Scale (FMPS),Hewitt Multidimensional Perfectionism Scale (HMPS),Slaney Almost Perfect Scale-Revised (APS-R),Rosenberg Self-esteem Scale (SES) and Beck Depression Inventory (BDI).Results Maladaptive perfectionism was positively correlated with depression (r =0.54,P ＜ 0.01),and negatively correlated with self-esteem (r =-0.55,P ＜ 0.01).Adaptive perfectionism was not correlated with depression (r =-0.09,P ＞ 0.05),and was positively correlated with self-esteem (r =0.36,P ＜0.01).Self-esteem partially mediated the association between maladaptive perfectionism and depression.The partial mediation effect was significant (P ＜0.05).The mediation model fitted the data well (GFI =0.986,CFI =0.988,NFI =0.981,TLI =0.970,RMSEA =0.075).Conclusion Self-esteem has partially mediating effect on perfectionism and depression.

Objective To analyze the correlation between pressure ulcer during operation and operation time under lateral operation position.Methods 89 operation patients all adopted lateral position.Pressure ulcer during operation was observed and operation time was registered.Results The operations went on smoothly.Operation time lasted 1 to 8 hours.There were 42 operations exceeded 4 hours and pressure ulcer during operation was observed in 15 patients.Positive rate was 35.7％.There were 47 operations lasted less than 4 hours and pressure ulcer during operation was observed in 6 patients.Positive rate was 12.8％.Statistical differences were significant between the two groups.Conclusions For those operation patients with lateral position whose operation time would be longer,especially more than 4 hours,nursing intervention measures should be adopted to prevent pressure ulcer during operation.

Since 2015, US government announced the Precision Medicine Initiative, the concept of“precision medicine” is spreading rapidly, which has seemingly become the mainstream of future medicine.Laboratory medicine based on personalized information brought by the development of precision medicine will be an entirely new area, however its complexity is far beyond the current knowledge and laboratory medical scientists are encouraged to be actively involved in this emerging field.Opportunities and challenges may coexist, which mainly include: screening and evaluation of circulating biomarkers, selection and standardization of appropriate molecular diagnostic techniques, acquisition of high quality biological specimens and establishment of sample library, and comprehensive interpretation of the results in order to achieve and improve individualized precise prevention, diagnosis, and treatment for specific diseases and patients.

Thirty caaes diagnosed as lGA nephroparthyby kidney biopay were treated bassed on differential diagnosis of syndromes the cases were divided into several stages via lesion in the lung -kidney (initial stage )lesion in the spleen-kidney (stable atage )and lesion in the liver -kidney (stable atage)and relapsing stage, and were treated by Chinese medicaments accordingly. The results revealed that 10 cases were totally mitigated; 13 cases, markedly alleviated; 4 cases, ameliorated, with only 3 cases ineffective. The total effecitve rate was 90%.Thirty cases diagnosed as IgA nephropathy by kidney biopsy were treated based on differential diagnosis of syndromes. The cases were divided into several stages, viz. lesion in the lung -kidney ( initial stage) , lesion in the spleen - kidney (stable stage) and lesion in the liver -kidney (advanced stage) and relaps-

OBJECTIVE:To explore the general regulation and characteristics of adverse drug reactions(ADR)induced by ti-cagrelor,and provide reference for clinical rational drug use. METHODS:672 patients received ticagrelor in our hospital from Dec. 2013 to Jun. 2015 were followed-up,and the situation was analyzed and summarized after taking drug. RESULTS:In the 672 patients,78(11.6%)patients sutfered from 108 cases of ADR,the ratio of male to female was 5.5∶1,there was statistical signifi-cance (P<0.05);patients mostly aged 51-60 years (37.2%);the mainly involved organs/systems were respiratory system (41.7%),and skin and its appendages (38.0%),the main clinical manifestations were minor bleeding (78.7%),dyspnea (17.6%) and bradycardia (2.8%);no severe ADR was found. CONCLUSIONS:Ticagrelor had good safety and tolerability,but due to its shorter time to market in China and absense of evidence-based medicine for large-scale clinical trials in Asian population, clinic should strengthen medication monitoring to reduce the ADR and ensure drug safety.

The present paper has reviewed and researched on 112 gynecological records from volume 17 ofZhang-Yuqing’s medical records, and has found that there were 9 common methods of treating gynecological diseases, namely dredging and regulatingqi and blood, adjusting qi to nourish ying, soothing the liver wood, harmonizing the liver and spleen (stomach), tonifying spleen and kidney, nourishing liver and kidney, regulating vital and conception vessels, eliminating dampness and phlegm, and nourishing Yin and clearing away heat.The treatment of gynecological diseases reflects the holistic concept and dialectical concept, and the valuable experience still has a high theoretical value, as well as clinical value, which is worth exploring and discussing.

OBJECTIVE To investigate the apoptosis of hepatocytes in arsenic poisoning rats caused by coal-burning and explore the effects of p53-induced mitochondrial apoptotic pathway on arsenic liver injury.METHODS Wistar rats were fed with 164.74 pp m arsenic conta minated grain at the levels of 15%,30% and 60% (arsenic contents were 25,50 and 100 mg·kg -1 ,respectively)for 90 d. The arsenic contents of urine and hair,apoptosis of hepatocytes and mRNA expression of p53,Bax and Bcl2 in peripheral blood and hepatocytes were evaluated.At the sa me ti me,protein expression of p53, Bax and Bcl2 in hepatocytes were analyzed.RESULTS The arsenic contents of urine and hair increased with the elevation of arsenic dose.The apoptotic rate of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 16.49 ±2.06 and 15.83 ±1 .28,respectively which were significantly higher than the control group and arsenic 25 mg·kg -1 group (9.00 ±0.59 and 9.27 ±0.36,respectively,P <0.05).p53 mRNA expression of peripheral blood in arsenic 100 mg·kg -1 group was 2.69 ±1 .84 while p53 mRNA expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .63 ±0.28, 1 .91 ±0.38 and 1 .71 ±0.18,respectively which were significantly higher than the control group (0.86 ± 0.15 and 1 .22 ±0.12,respectively,P<0.05).Bax mRNA expression of peripheral blood in arsenic 50 and 100 mg·kg -1 group were 1 .36 ±0.30 and 1 .94 ±0.65 while Bax mRNA expression of hepatocytes in arsenic 100 mg·kg -1 group was 1 .34 ±0.23 which were significantly higher than the control group (0.77 ±0.15 and 0.84 ±0.34,respectively,P<0.05).Bcl2 mRNA expression of hepatocytes in arse-nic 100 mg·kg -1 group was 0.98 ±0.50 which was significantly lower than the control group (2.14 ± 1 .15,P<0.05).p53 protein expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .06 ±0.56,1 .15 ±0.77 and 0.74 ±0.27,respectively while Bax protein expression of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 0.74 ±0.43 and 0.69 ±0.37 which were significantly higher than the control group (0.36 ±0.1 9 and 0.25 ±0.09,respectively,P<0.05).CONCLUSION Arsenic can induce hepatocytes apoptosis and p53-mediated mitochondrial apoptotic pathway may be involved in the develop ment of rat liver injury in arsenic poisoning rats caused by coal-burning.

Objective In previous experiment we proved that rat marrow stromal cells(MSCs) could be induced to differentiate to neuron\|like cells by ? mercaptoethanol and all\|trans\|retinoic acid(ATRA), but the neuron\|like cells showed apoptosis sooner after the differentiation.In this study, we used several sets of combinations of anti\|apoptotic reagents and inductive reagents to induce the differentiation of MSCs,to prevent the differentiated cells from apoptosis and to elongate the lifetime of the neuron\|like cells which might be use in clinic. Methods The MSCs were isolated primarily from bone marrow of adult rat,and purified by passage culture.The 5th passage of MSCs was induced by single or combinative induction,which included RA,Melatonin(10 -9 mol/L), bFGF,bFGF+RA,Melatonin+RA,Melatonin+bFGF.At 1?h,5?h and 24?h of induction, the ratio of neuron\|like cells and the numbers of survived neuron\|like cells among 6 groups were compared.In addition,immunocytochemical stainings with anti\|MAP\|2 and GFAP were also performed on cells of 5?h of induction. Results Neuron\|like cells were all MAP\|2 positive,but GFAP negative.The ratios of neuron\|like cells in MSCs at 1?h after induced by six combinations of reagents were 0 24,0 28,0,0,0 44,0 64;at 5?h were 0 56,0 28,0,0,0 76,0 83 and at 24?h were 0 10,0 27,0,0 14,0 35,0 59 respectively.The ratios of survived neuron\|like cells of differentiated cells at 24?h after induction by RA,Melatonin,Melatonin+RA and Melatonin+bFGF were 0 08,0 37,0 05,0 51 respectively.Conclusions\ 1.bFGF can't induce MSCs into neuron\|like cells independently.Moreover,it suppressed the differentiation effects of RA,while enhancing the effects of MT.2.MT can induce MSCs differentiating into neuron\|like cells and has the anti\|apoptotic effects on the induced neuron\|like cells.3.When MT and bFGF were added together,not only the ratio of neuron\|like cell in MSCs was increased, the lifetime of neuron\|like cells was also elongated.

Aim The present study is designed to elucidate the protective effect of Polysaccharide from Spirulina Platensis(PSP) on dopaminergic neurons of MPTP-induced Parkinsons disease model in C57BL mice.Methods C57BL mice were divided into 5 groups:control group,MPTP group,PSP 200 mg?kg-1 plus MPTP group,PSP 400 mg?kg-1 plus MPTP group,and PSP 800 mg?kg-1 plus MPTP group.Immunohistochemistry assay,RT-PCR,and HPLC-ECD were used to detect the immunoreactivity of tyrosine hydroxylase(TH) and dopamine transporters(DAT),the expression of TH mRNA and DAT mRNA in substantia nigra(SN),the content of dopamine(DA) and its metabolites,i.e.3,4-dihydroxyphenyl acetic acid(DOPAC) and homovanilic acid(HVA) in striatum(Str).Results The result showed that,compared with control group,the TH and DAT immunoreactive stain in MPTP group was significantly decreased(P

Objective To explore the clinical effect of treating the ischemia of vertebral-basilar arteries by venous infusion of Yinxingdamo.Methods 116 cases of the patients suffering from the ischemia of vertebral-basilar arteries were divided randomly into two groups.In study group,66 patients were infused 20ml of Yinxingdamo injection added in 0.9% NS 250ml by I.V. q.d. for two weeks.In controlled group 50 patients were infused 20ml of compound danshen injection in 250ml 0.9% NS by I.V q.d. for two weeks.Results In study group,the symptoms of 55 cases were completely remissive,6 cases significantly improved,4 cases mildly improved,and no improvement was found in one case.In controlled group,the symptoms of 5 cases were completely remissive,18 cases significantly improved,16 cases mildly improved,and no improvement was found in 11 cases.In study group the total efficacy rate of 98.48% was significantly higher than that of controlled group which was 78%(P