AIM: To investigate the protective mechanism of Danggui Buxue Decoction(Radix Astragali,Radix Angelieae Sinensis) on the kidney of diabetic rats. METHODS: The diabetic animal model was made by a single intraperitoneal injection of 60 mg/kg streptozotocin(STZ) and treated with Danggui Buxue Decoction(DBD) or Benazepril. Renal weight/body weight ratio, blood glucose and lipid, urinary albumin and ? 2-microglobin were measured. The renal pathological lesions were also investigated. The expression of TGF-? 1 mRNA in renal cortex as well as Angiotensin Ⅱ(AngⅡ) concentration of plasma and kidney tissue were determined by In situ hybridization and radioimmunoassay after 4,8 weeks of treatment. RESULTS:DBD had equivalent effects with Benazepril in protecting renal function and alleviating pathologic lesions. By means of inhibiting the expression of of TGF-? 1 mRNA. DBD had no effect on renal and plasmaAngⅡ. CONCLUSION:Danggui Buxue Decoction may alleviate kidney lesion and slow down the progression of DN by decreasing the renal expression of TGF-? 1 mRNA.

Objective To investigate the whole differentiating process of myogenic satellite cells in vitro,and the expression of N-ACh receptors.Methods Skeletal muscle satellite cells were isolated by trypsin digestion in vitro and purified by preferential attachment method.The cells were observed morphologically and identified by ?-sarcomeric actin,desmin and skeletal myosin antibodies.Contraction of myotubes was observed after adding ACh in the culture medium, N-AChRs in myotube were also be identified by bungarotoxin.Results Cultured satellite cells in vitro differentiated into myoblasts and fused to form myotubes later.These cells were positive by ?-sarcomeric actin, skeletal myosin and desmin antibodies' staining.Adding ACh directly in the medium stimulated the contraction of myotubes.No obvious expression of ACh receptors was observed on the surface of satellite cells.Cluster of receptors aggregated on myotubes from neonatal rats' satellite cells cultured in differentiation media.Conclusion The myotubes differentiated from myogenic satellite cells have the function of contraction.N-ACh receptors were synthesized gradually during the process of differentiation.

Aim The present study is designed to elucidate the protective effect of Polysaccharide from Spirulina Platensis(PSP) on dopaminergic neurons of MPTP-induced Parkinsons disease model in C57BL mice.Methods C57BL mice were divided into 5 groups:control group,MPTP group,PSP 200 mg?kg-1 plus MPTP group,PSP 400 mg?kg-1 plus MPTP group,and PSP 800 mg?kg-1 plus MPTP group.Immunohistochemistry assay,RT-PCR,and HPLC-ECD were used to detect the immunoreactivity of tyrosine hydroxylase(TH) and dopamine transporters(DAT),the expression of TH mRNA and DAT mRNA in substantia nigra(SN),the content of dopamine(DA) and its metabolites,i.e.3,4-dihydroxyphenyl acetic acid(DOPAC) and homovanilic acid(HVA) in striatum(Str).Results The result showed that,compared with control group,the TH and DAT immunoreactive stain in MPTP group was significantly decreased(P

Objective To explore the clinical effect of treating the ischemia of vertebral-basilar arteries by venous infusion of Yinxingdamo.Methods 116 cases of the patients suffering from the ischemia of vertebral-basilar arteries were divided randomly into two groups.In study group,66 patients were infused 20ml of Yinxingdamo injection added in 0.9% NS 250ml by I.V. q.d. for two weeks.In controlled group 50 patients were infused 20ml of compound danshen injection in 250ml 0.9% NS by I.V q.d. for two weeks.Results In study group,the symptoms of 55 cases were completely remissive,6 cases significantly improved,4 cases mildly improved,and no improvement was found in one case.In controlled group,the symptoms of 5 cases were completely remissive,18 cases significantly improved,16 cases mildly improved,and no improvement was found in 11 cases.In study group the total efficacy rate of 98.48% was significantly higher than that of controlled group which was 78%(P

Full ceramic restorations become more and more popular for their excellent properties. Bond strength between ceramic and composite resin cement is the key factor to success in clinical use. This paper illuminated the importance of ceramic surface treatments and reviewed recent progresses on ceramic surface treatments. The difference of bond strength caused by different dental materials was discussed too.

Objective To assess the velocity patterns of right ventricular long axis myocardial motion during isovolumic contraction phase in the patients with dilated cardiomyopathy(DCM) by Doppler tissue imaging(DTI).Methods Right ventricular systolic free wall myocardial(the place close to the tricuspid annular) motion velocities were examined by DTI on apical four-chamber view in 28 patients with DCM and 30 healthy volunteers.Peak positive and negative velocities(V_(IVC1),V_(IVC2)),velocity time integrals(VTI_(IVC1),VTI_(IVC2)),times(T_(IVC1),T_(IVC2)) of right ventricular free wall myocardial motion during isovolumic contraction,and the peak velocity(V_S),time(T_S),velocity time integral(VTI_S) of ejection wave(S) were measured.Right ventricular percent changes in area(RVPCA) on three different views were calculated.Results V_(IVC1),V_S,VTI_S and differential IVC(the difference between V_(IVC1) and V_(IVC2)) in the patients with DCM were significantly decreased than those in control group(P(0.05)).Conclusions The changes of DTI indices of right ventricular long axis myocardial motion during isovolumic contraction in the patients with DCM are characteristic,which could be a new method to evaluate right ventricular systolic function.

OBJECTIVE:To investigate the knowledge of parents on children's drug-use safety in a community in order to provide basis for development of children's drug-use safety education.METHODS:From Jul.to Dec.in 2008,parents who took their children aged from 3 months old to 6 years old to our center to receive child care examination or preventive injections were enrolled.The self-made questionnaire surveys were answered by subjects on the spot.The survey involved subjects' general information,basic knowledge on drug-use safety and main source of the knowledge,etc.RESULTS & CONCLUSIONS:382 pieces of questionnaire were delivered and 380 pieces of valid questionnaire were returned.The correct rate of parents' answers about drug-use safety was 71.5%.Parents who had higher academic qualifications were superior to those who had lower academic qualifications in respect of correct rate (P

AIM:To supply the theoretic evidences of elongating the lifetime of neuron-like cells differentiated from adult rat mesenchymal stem cells, we investigated the relationship between the differentiation and apoptosis in the process of induction. METHODS: The mesenchymal stem cells(MSCs) were isolated primarily from rat bone marrow, and purified by passage culture. The 5th passage of MSCs was induced by ?-mercaptoethanol and all-trans-retinoic acid (ATRA). After 1 h, 3 h and 5 h of induction, the cells were stained immunocytochemically with anti-MAP-2 and anti-GFAP antibodies, respectively. In addition to counting the ratio of neuron-like cells in MSCs, DAPI staining was employed to identify whether the differentiated cells have an apoptotic morphological changes. The ratio of apoptotic cells at 1 h, 3 h and 5 h after induction were detected by flow cytometry (FCM). RESULTS:Neuron-like cells appeared among MSCs and could be identified by MAP-2 staining 30 min after MSCs being treated by ?-mercaptoethanol and ATRA. However, 5 h after induction, some of these neuron-like cells began to float and die. With DAPI staining, the nuclei of some dying cells were shown to be condensed and later fragmented. The ratios of cells undergoing apoptosis at 1 h,3 h and 5 h after induction by ?-mercaptoethanol and ATRA were 1.5%?6.1%?21.5% and 1.5%?1.7%?12.3%, respectively . CONCLUSIONS:1. ?-mercaptoethanol and ATRA had the different ability that induced MSCs to differentiate to neuron-like cells. 2. Apoptosis was also initiated in the process of differentiation, and there is positive correlation between the ratio of differentiation and apoptosis.

Triple-negative breast cancer ( TNBC ) is a subtype of breast cancer,and it is characterized by an aggressive clinical course with a poor prognosis.Treatment for TNBC has attracted much attention in recent years,however,there is no standard treatment for TNBC in clinical setting.The pCR rates of neoadjuvant chemotherapy in TNBC ranges from 12％ to 48％ in the current published data,and it is higher than that in other types of breast cancer,however,the fluctuating range of the TNBC ’s pCR is large in literature.Although the administration of adjuvant chemotherapy for early TNBC is controversial,the regimen without anthracyclines is reported to be suitable for early TNBC patients.Standard cytotoxic agents including taxanes and anthracyclines are still the main choices for TNBC salvage treatment,and the combination with gemcitabine or capecitabine may improve the overall survival.Poly (ADP-ribose) polymerase (PARP) is a new molecular target for TNBC in ongoing studies.Further research on the target-inhibitors such as BSI-201and Olaparib will provide more effective choices to clinical treatment.

OBJECTIVE To investigate the apoptosis of hepatocytes in arsenic poisoning rats caused by coal-burning and explore the effects of p53-induced mitochondrial apoptotic pathway on arsenic liver injury.METHODS Wistar rats were fed with 164.74 pp m arsenic conta minated grain at the levels of 15%,30% and 60% (arsenic contents were 25,50 and 100 mg·kg -1 ,respectively)for 90 d. The arsenic contents of urine and hair,apoptosis of hepatocytes and mRNA expression of p53,Bax and Bcl2 in peripheral blood and hepatocytes were evaluated.At the sa me ti me,protein expression of p53, Bax and Bcl2 in hepatocytes were analyzed.RESULTS The arsenic contents of urine and hair increased with the elevation of arsenic dose.The apoptotic rate of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 16.49 ±2.06 and 15.83 ±1 .28,respectively which were significantly higher than the control group and arsenic 25 mg·kg -1 group (9.00 ±0.59 and 9.27 ±0.36,respectively,P <0.05).p53 mRNA expression of peripheral blood in arsenic 100 mg·kg -1 group was 2.69 ±1 .84 while p53 mRNA expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .63 ±0.28, 1 .91 ±0.38 and 1 .71 ±0.18,respectively which were significantly higher than the control group (0.86 ± 0.15 and 1 .22 ±0.12,respectively,P<0.05).Bax mRNA expression of peripheral blood in arsenic 50 and 100 mg·kg -1 group were 1 .36 ±0.30 and 1 .94 ±0.65 while Bax mRNA expression of hepatocytes in arsenic 100 mg·kg -1 group was 1 .34 ±0.23 which were significantly higher than the control group (0.77 ±0.15 and 0.84 ±0.34,respectively,P<0.05).Bcl2 mRNA expression of hepatocytes in arse-nic 100 mg·kg -1 group was 0.98 ±0.50 which was significantly lower than the control group (2.14 ± 1 .15,P<0.05).p53 protein expression of hepatocytes in arsenic 25,50 and 100 mg·kg -1 group were 1 .06 ±0.56,1 .15 ±0.77 and 0.74 ±0.27,respectively while Bax protein expression of hepatocytes in arsenic 50 and 100 mg·kg -1 group were 0.74 ±0.43 and 0.69 ±0.37 which were significantly higher than the control group (0.36 ±0.1 9 and 0.25 ±0.09,respectively,P<0.05).CONCLUSION Arsenic can induce hepatocytes apoptosis and p53-mediated mitochondrial apoptotic pathway may be involved in the develop ment of rat liver injury in arsenic poisoning rats caused by coal-burning.