Objective To explore the effect of treadmill training on expression of glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) after cerebral ischemia-reperfusion in rats. Methods 30 male Wistar rats were randomly divided into sham group, model group and treadmill training group, with 10 rats in each group. The latter 2 groups were modeled with middle cerebral artery occlusion for 2 hours and reperfusion. The treadmill training group underwent treadmill exercise on the 3rd day after modeling for 12 days. Neurological function was evaluated with modified Neurological Severity Scores (mNSS). The neuronal pathological change in ischemic cortex was observed with HE staining. The expressions of GFAP and BDNF in cortex were determined by Western blotting. Results Com-pared with the model group, the mNSS scores decreased in the treadmill training group (F=9.931, P<0.01), the pathological damage in the ischemia cortex significantly lessened, and the expressions of GFAP (t=6.73) and BDNF (t=3.78) increased (P<0.05). Conclusion Treadmill training may increase the expression of GFAP and BDNF after cerebral ischemia-reperfusion in rats, and promote the recovery of neurologi-cal function.

Objective To investigate the clinical efficacy of acupuncture in treating subhealth.Methods One hundred and fifty-four patients with subhealth were allocated, using a random number table, to observation and control groups, 77 cases each. The control group received basic treatment by diet, exercise, rest and rest and psychological intervention and the observation group, acupuncture therapy in addition. Subhealth scale subscores and the 36-Item Short-Form Health Survey (SF-36) subscore were recorded and blood biochemical indicators were measured in the two groups before and after treatment. the clinical therapeutic effects were compared between the two groups.Results The total efficacy rate was 96.1% in the observation group and 83.1% in the control group; there was a statistically significant difference between the two groups (P<0.05). There were statistically significant pre-/post-treatment differences in subhealth scale subscores in the two groups (P<0.05). After treatment, sleep, digestion, urination, positive emotion, ability and social relation subscores were higher, and fatigue, discomfort and negative emotion subscores were lower in the observation group than in the control group (both P<0.05). There were statistically significant pre-/post-treatment differences in blood biochemical indicators in the two groups (P<0.05). After treatment, blood uric acid (UA), total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) were significantly lower and high-density lipoprotein cholesterol (HDL-C) was significantly higher in the observation group than in the control group (both P<0.05). The 36-Item Short-Form Health Survey (SF-36) subscore were higher in thetwo groups after treatment than before treatment (P<0.05). After treatment, physiological function, role-physical, role-emotional, body pain and general health subscore were significantly higher in the observation group than in the control group (P<0.05).Conclusions Acupuncture helps to improve subhealth status in population, regulate blood biochemical indicators and enhance the clinical therapeutic effect to raise the quality of life in the patients.

Objective:To explore the relationship between the latent classes of online emotion regulation among college students and the distribution difference of different types on variables such as gender,grade,ages of surfing the net and so on.Methods:Totally 421 college students were assessed with Online Emotion Regulation Questionnaire (OERQ).Latent class analysis was used to identify online emotion regulation classes,and then analyzed the distribution of latent classes among different genders,grades and ages of surfing the net.Results:Two latent classes of online emotion regulation were identified,including online emotion inhibiting/ignoring class (37.6％) and online emotion venting class (62.4％).The prevalence of online emotion venting class among girls (56.6％ vs.3.8％),sophomores (36.3％ vs.11.9％),juniors (23.4％ vs.3.1％),those with more than 36 months of surfing the net (31.6％ vs.18.8％) were significantly higher than the other of the respective kind.Whereas,the rate of inhibiting/ignoring class among boys (32％ vs.7.6％),freshmen (20％ vs.5％) were significantly higher.Conclusion:It suggests that online emotion regulation could be divided into two different latent classes and prevalence of each class is significantly different in different genders,grades,and ages of surfing the net.

Objective:To explore the correlation between fibrinogen levels and chemotherapy effect of platinum drugs in ovarian cancer patients. Methods:Totally 91 ovarian cancer patients and 95 patients with benign ovarian tumor were involved in our retrospec-tive study, and the associations between preoperative fibrinogen levels and clinic pathologic parameters in the patients were analyzed. Results:High fibrinogen levels were observed in 36.26％ (33/91) of the patients with ovarian cancer ( >4.0 g·L-1), which was significantly higher than that in the patients with benign tumor(P<0. 05). The high fibrinogen levels in ovarian cancer patients were associated with the higher FIGO stage and poor chemo-response, while were not associated with the patients' age, CA-125 levels and histological type and grade (P>0. 05). More importantly, no correlation was observed between high blood fibrinogen and CA-125 lev-els (P>0.05). Chemotherapy resistance was more in the patients with high fibrinogen levels than that in the other patients(P <0. 05). The OR value of high fibrinogen levels was 3. 571, 95％ CI was 1. 276-10. 000, which suggested high fibrinogen level is a dangerous factor for chemotherapy resistance. Conclusion:The study suggested that elevated fibrinogen level was a dangerous factor for chemotherapy response, which has a potential role in the poor prognosis of ovarian cancer patients independent of CA-125.

Animals ; Cytokines ; physiology ; Humans ; Immune System ; drug effects ; physiology ; Melatonin ; pharmacology ; Opioid Peptides ; physiology ; Receptors, Melatonin ; physiology ; Stress, Physiological ; immunology ; T-Lymphocytes ; drug effects ; physiology

BACKGROUND The effect of lipid-regulating therapy has been affirmed definitely in the first and second grade prevention of coronary arteriosclerotic cardiopathy, and Ginkgo biloba extract has a certain effect to lower blood lipid.OBJECTIVE: To investigate the effect of new Ginkgo biloba extract recipe (NGBR) on the blood-lipid level and the degree of hepa tic fatty degeneration in experimental hyperlipidemic rats. DESIGN:A randomized and controlled trial.SETTING: Department of Cardiology , Jinshan Hospital of Fudan University; Suzhou Institute of Traditional Chinese Medicine, Department of Pharmacology, Soochow University,MATERIALS: A total of 60 SD rats of clean grade, after one week adaptive feeding, were randomly divided into six groups: normal control group, hyperlipidemic model group, NGBR 20 mg/(kg·d), 40 mg/(kg·d)and 80 mg/(kg·d) groups, as well as lipanthyl 25 mg/(kg·d)group, with 10 in each group. 10METHODS: The rats in lipanthyl 25 mg/(kg·d), NGBR 20 mg/(kg·d), 40 mg/(kg·d) and 80 mg/(kg·d) groups were by gavage administrated 10 mL/kg, at 9:00-10:00 every morning; while the rats in normal control group and hyperlipidemic model group were given distilled water of the same volume. Except for the rats in normal control group, the rats in other five groups were by gavage given fatty emulsion 10 mL/kg, at 1:00-2:00every afternoon for 28 days consecutively. On the 29th day, all the rats were tested on serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), apolipoprotein AI (apoAI) and apolipoprotein B (apoB); at the same time the lives of the rats were taken out for pathological.examination of the degree of hepatic fatty degeneration. MAIN OUTCOME MEASURES:The blood-lipid changes and the degree of hepatic fatty degeneration of rats in each group RESULTS:Totally 60 rats involved all entered the final result analysis. ① Comparison of blood-lipid changes of rats in each group: The levels of TC and low density lipoprotein cholesterol (LDL-C) in hyperlipidemic model group were obviously increased, HDL-C level was decreased, as compared with those in normal control group there was a significant difference (P < 0.01); but the TG level was only slightly increased (P > 0.05). In the three NGBR groups the levels of TC, TG and LDL-C were obviously decreased(P < 0.05 or 0.01 ), and the levels of HDL-C, apoAI and apoB were increased to a certain grade, with in the NGBR 80 mg/(kg·d)group the difference being obvious(P < 0.05). ② Comparison of the degree of hepatic fatty degeneration of rats in each group: In pathological examination, the liver of rats in hyperlipidemic model group presented diffuse fat vacuoles; in NGBR 40 and 80 mg/(kg·d)groups, the degeneration levels reduced to a given degree, especially obvious in NGBR 80 mg/(kg· d)group. CONCLUSION:NGBR can effectively correct the lipid metabolism in hyperlipidemia, with a good preventive role to hyperlipidemia induced by feeding fatty emulsion.

Objective To investigate the effects of patient controlled epidural analgesic(PCEA) used ropivacaine and different analgesic drugs on recovery of postoperative intestinal peristalsis.Methods 105 patients of abdominal surgery undergoing selection operating,ASA Ⅰ,Ⅱ grade.Every section was randomly divided into three groups according to the content of drugs.Morphine group:0.125％ ropivacaine + 0.005％ morphine + 0.005％ droperidol.Fentanyl group:0.125％ ropivacaine +0.0005％ fentanyl +0.005％ droperidol.Control group is not used PCEA.All patients were taken PCEA for 36 hours.The pain scores of visual analog scale ( VAS),sedative scores of Ramsay scale,respiratory and hemodynamic paremeters,and side effects were recorded at 4,8,12,24,36 hours postoperatively.The time of the recovery of intestinal peristalsis was also recorded.Results Good analgesic effect could be found in morphine group and fentanyl group,the VAS grades were significantly lower in morphine group and fentanyl group than that in control group( P ＜ 0.01 ).The time of the recovery of intestinal peristalsis was shorter in fentanyl group than that in morphine group and control group respectively ( P ＜ 0.05 ).Conclusion Compound solution with low concentration of ropivacaine and morphine or fentanyl can be used for postoperative analgesia,and ropivacaine combined with fentanvl can shorten the time of the recovery of intestinal peristalsis.

Objective To explore the feasibility of the small figure-L unilateral transternal incision for the treatment of anterior mediastinal tumor and cyst. Methods Eighteen patients with anterior mediastinal tumor or cyst were enrolled in the study. Under general anesthesia, after a 8 to 10 cm middle skin incision was made, a unilateral figure-L partial sternotomy on the side occupied by the tumors was made in the third or forth intercestals space,taking into account the tumors size and location. Tumors were resected with excellent exposure. Results All but one tumors were completely resected during the operation. In 3 cases,tumors encroached on the adjacent lung tissue,and lung tissue was partially resected. In 2 cases, tumors invaded the pericardium, and the pericardium was partially removed.Mean operating time was 120 minutes( 80～140 minutes). The mean time of hospital stay was 10 days after the operation, and there was no complications. All patients were alive with a mean follow-up period of 24 months ( 8～36 months). Conclusion The figure-L unilateral sternotomy is an effective and useful minimally invasive approach for anterior mediastinal tumors.

The technology of treating tumors utilizing electric fields is a newly rising therapeutic method for carcinomas,which shows the features of high efficiency,no remanent toxicity,being easily regulated and high applicability.However,neither the anti-tumor mechanisms are well studied nor the parameters for praetical ap-plication is defined.Therefore the technology has become a brightened spot already,the major methods of which include direct current electric fields.pulsed electric fields and tumor treating fields(TT-Fields).

The incidence of gastrointestinal diseases increases every year.Etiology and pathogenesis of gastrointestinal diseases have been not yet elucidated,such as inflammatory bowel disease,gastrointestinal cancers,and allergic diseases.In recent years,the role of mucin genes expression in gastrointestinal diseases has been paying much attention to.Studies have shown that mucins are the main component of mucus in the digestive tract,and a major factor in the formation of the gastrointestinal barrier.Mucins are related to gastrointestinal epithelium,intestinal microflora and inflammatory factors.Abnormal expression of gastrointestinal mucin genes,especially MUC1 and MUC2,are related to Gastrointestinal inflammation and cancer.Currently gastrointestinal mucin genes expression contribute to exploring the diagnosis,treatment and prognosis of digestive diseases.This study is to summarize the progress in gastrointestinal mucin genes expression.