ObjectiveTo observe the relationship between the mean platelet volume (MPV) and stent thrombosis(ST) in patients after percutaneous coronary interventional procedures (PCI),to determine a concise and high-risk indicator to predict thrombosis in the patients after PCI.MethodsTo determine the MPV from 229 patients after PCI.The patients with MPV significantly increased were sekected as the observation group,the patients with normal MPV were selected as the control group.The stsituation in the two groups of ST was observed.The values in the two grups were analyzed.ResultsThe ST rate of control group was 1.22％,which was lower than that of the observation group(6.15％ )( x2 =4.02,P ＜ 0.05 ).ConclusionThe value of MPV increased was a risk factor for patients with thrombosis after PCI.Taking effective and timely adjustment of antithrombotic treatment options had an important clinical significance to prevent ST occur in patients after PCI.

Objective To compare the traditional anterior transposition and anterior subcutaneous with blood supply of the ulnar nerve for the treatment of cubital tunnel syndrome.Methods Eighty cases with cubital tunnel syndrome were enrolled in the retrospective study from January 2009 to March 2013,40 cases (control group) were treated by traditional anterior transposition while the others (observation group) were treated by anterior subcutaneous with blood supply.According to the evaluation criteria of ulnar nerve,evaluated the function of patients and compared the scores of two groups and the improved rate of different level patients.Results The scores of two groups before surgery were (3.6 ± 0.4),(3.7 ± 0.5) scores,after surgery were (7.5 ± 0.9),(7.4 ± 0.6) scores respectively,there was significant difference between two groups before and after surgery (P ＜ 0.05),but there was no significant difference between two groups (P ＞0.05).The improved rate of observation group was 87.5％ (35/40),which was higher than that of control group [85.0％ (34/40)],but without statistical difference (P ＞ 0.05).In two groups the patients with middle scores showed no statistical significance in improved rate [90.0％ (27/30) vs.96.4％ (27/28)] (P ＞ 0.05).Two treatments showed a significant difference for the low scores patients 8/10 vs.7/12 (P ＜0.05).Conclusion Anterior subcutaneous with blood supply of the ulnar nerve is better than traditional anterior transposition for the gross cubital tunnel syndrome patients.

ObjectiveTo determine the dose of butorphanol at which 50％ and 95％ effective dose (ED50 and ED95) of patients inhibition uterine contraction pain on analgesic artificial abortion.Methods Twenty-six patients undergoing analgesic artificial abortion were sequentially given different doses butorphanol so that the ED50 and ED95 could be determined by up-and-down sequential test.Anesthetic depth was observed when giving propofol with 200 mg/min speed,uterine contraction pain at awaking immediately,10,20 min after awaleing.Data was analyzed by Probit regression analysis for calculating ED50 and ED95..ResultsButorphanol could restrain uterine contraction pain on analgesic artificial abortion and the ED50 was 246 μg with the 95％ confidence interval of 201 μg to 281 μg,the ED95 was 324 μg with the 95％ confidence interval of 287 μg to 548 μg.ConclusionED50 and ED95 value can be expected as a parameter to optimize analgesic artificial abortion.

After an evaluation system of intellectual property rights in tea industry was constructed according to the investigation of literature and networks , the development of intellectual property rights in tea industry of China from 2004 to 2013 was analyzed , which showed that although the comprehensive strength of intellectual property rights in tea industry of China has steadily increased , a number of problems still exist such as its copyright , trademark rights, geographical indications, intangible cultural heritage, and new plant strain rights.Further analysis of patent invention rights, practical patents, and outward appearance designs showed that the number of tea processing patent inventions is the largest with a stable patent right, followed by tea processing and tea sets, tea sets and tea pack-age.Suggestions were put forward for the development of intellectual property rights in tea industry of China.

Indicators for evaluation of Chinese medicine intellectual property rights evaluation system were selected interms of patent right, copyright, trademark right, geographical mark, protection of Chinese medicine, and intangible cultural heritage to construct a Chinese medicine intellectual property rights evaluation system which is consisted of 6 class A,15 class B and 18 class C indicators .Domestic data of Chinese medicine intellectual property rights were col-lected from 2004 to 2013,the weight of each evaluation indicator was determined using standard deviation method,and the evaluation system was studied according to the weighted TOPSIS method with its rationality and practicality validated.

ObjectiveTo discuss the mechanism of promotion of gastrointestinal motility during multiple organ dysfunction syndrome ( MODS ) by Dachengqi decoction, by examining the expression of Bcl-2, Bax of mitochondrial pathway, and nuclear factor-κB (NF-κB) in smooth muscle of the small intestinal in rats.Methods According to the random number table, 100 healthy adult Wistar rats were divided into three groups: control group with 20 rats, model group with 40 rats, and Dachengqi decoction group with 40 rats. Rat model of MODS was reproduced by bacterial peritonitis induced by an injection of 1 mLEscherichia coli suspension (8×108 cfu/mL) into peritoneal cavity. The rats in control group were given 1 mL normal saline intraperitoneally. The rats in Dachengqi decoction group were given 10 mL/kg Dachengqi decoction by gavage, twice a day, before inoculation of the bacterial suspension. Twenty-four hours after modelling, rats in all groups were sacrificed by cervical vertebra luxation, and the upper small intestine was harvested to detect the protein expressions of Bcl-2, Bax, and NF-κB in smooth muscle tissue using immunohistochemical staining.Results In the control group, a large amount of Bcl-2 protein was expressed and it was distributed uniformly in small intestinal smooth muscle. On the other hand, a small amount of Bax and NF-κB protein was expressed, and they were also distributed uniformly. Compared with the control group, Bcl-2 protein was distributed only sparsely, and it was scattered in intestinal smooth muscle in blocks in the model group. The expression of Bcl-2 protein was obviously down-regulated [integral optical density (A) value: 7 115.3±1 797.2 vs. 22 085.5±4 892.2, P< 0.05], and this phenomenon was more prominent in circular muscle layer. Bax and NF-κB were densely distributed, and their expressions were upgraded obviously [Bax (A value): 33 802.6±5 778.0 vs. 7 984.4±1 804.5, NF-κB (A value): 2 465.9±664.8 vs. 1 572.6±256.0, bothP< 0.05]. This phenomenon was more outstanding in circular muscle layer. Compared with that of the model group, the expression of Bcl-2 protein was stronger obviously in intestinal smooth muscle in Dachengqi decoction group (A value: 12 458.6±2 491.1 vs. 7 115.3±1 797.2,P<0.05). The expressions of Bax and NF-κB were down-regulated obviously [Bax (A value): 12 529.2±2 018.5 vs. 33 802.6±5 778.0, NF-κB (A value): 1 843.1±373.6 vs. 2 465.9±664.8, bothP< 0.05], and the change was more obvious in circular muscle layer.Conclusions Dachengqi decoction may promote recovery of gastrointestinal motility through an increase of Bcl-2 expression in nuclear membrane, thus preventing translocation of Bax to mitochondrion, thereby reduces mitochondrial damage in MODS.

Objective To investigate onthe correlation between the seven single nucleotide polymorphisms and genesis of T2DM. Methods High‐resolution melting (HRM ) curve technology was used to detect thegenotype of the seven SNPs in 202 T2DM patients and 200 healthy volunteers. The relationship between susceptible gene polymorphism and T 2DM was analyzed. Results The frequency of five SNP loci rs8050136 ,rs13266634 ,rs7578597 ,rs864745 and rs7961581 showed significant differences between T2DM patients and controls ( P< 0.01 ). The OR of rs8050136 ,rs13266634 ,rs757859 and rs864745 was >1 ,while OR of rs7961581 was <1. There was no significant difference in rs10811661 and rs10923931 loci between T2DM patients and controls (OR=1). Conclusion The polymorphism of loci rs8050136 ,rs13266634 ,rs7578597 and rs864745 is associated with T2DM genesis in this study population , while rs10811661 and rs10923931 is not associated with T 2DM genesis ,and rs7961581 may be a protective factor for T2DM.

Objective To determine the susceptibility of Ureaplasma urealyticum(Uu) to 7 kinds of antimicrobial agents, to compare the result with tetracycline resistant determinant(tetM) detected by PCR technique.Methods Forty two Uu isolates were subcultured for 3 generations and then cultured with drugs with a series of concentrations from 64?g/ml ～ 0 06?g/ml. MIC was determined when the color of culture medium did not change from yellow to red in 72h culture. The tetM determinant in the isolates was detected by PCR with specific primers, and PCR products were digested by Taq 1 restrictive endonuclease. Results The antimicrobial activity of tetracyclines to Uu was low. No Uu strains resistant simultaneously to 3 groups of antibiotics were found in the study. Detection rate of tetM was 71 4% with correspondent digested fragments. Conclusion The susceptibility of Uu isolates to tetracycline group was low, but higher to macrolides and fluoroquinolones. Uu strains carrying tetM determinant might become drug resistant ones which should be monitored and followed up with MIC determination.

Objective To investigate the curative efficacy of fracture with closed reduction and percutaneous Kirschner wire fixation of the simple lateral injury for humeral supracondylar fracture of Gartland type Ⅱ and type Ⅲ in children by the emergency treatment and selective surgery. Methods From March 2010 to March 2012,sixty children with Gartland type Ⅱ and type Ⅲ fresh humeral supracondylar fracture were operated in Xianyang Center Hospital. Six-ty children were randomly divided into two groups:group A by emergency operation 8 hours after injury with closed re-duction method and lateral percutaneous Kirschner wire fixation only,group B initially treated with plast fixation,then undergoing surgery after 3 - 5 days of injury with the closed reduction and percutaneous pin fixation. The operation du-ration,frequency of intraoperative image intensifier,postoperative swelling,pain lasting time after injury and hospitaliza-tion cost were investigated in two groups. Results The average operation duration of group A was(18. 5 ± 12. 3)min, and that in group B was(20. 1 ± 15. 3)min,and there was a statistically significant difference between two groups(P ﹤0. 05). The frequency of intraoperative image intensifier was 6. 2 times on the average in group A,but 7. 3 times in group B,and there was a statistically significant difference between two groups(P ﹤ 0. 05). The postoperative swelling rate on 3,5,7 days after injury in group B was significantly higher than that of group A,and there was a statistically sig-nificant difference(all P ﹤ 0. 05). The duration of pain after injury in group A was(3. 44 ± 1. 23)days,but(5. 26 ± 1. 36)days in group B,which was significantly different;the nerve function recovery time of group A was(36. 5 ± 7. 0) d,and it was(36. 2 ± 7. 0)d in group B,which was not significantly different(P ﹥ 0. 05). The average hospitalization time and cost in group A was less than that in group B,which was significantly different(P ﹤ 0. 05). Finally there was no statistically significant difference in elbow joint function between two groups of children(P ﹥ 0. 05). Conclusions The emergency operation of closed reduction and percutaneous Kirschner wire fixation for the treatment of simple lateral injury in children with Gartland type Ⅱ and type Ⅲ fracture has the advantages over the selective surgery,including shorter operation time,less contact of X - ray intraoperatively,light postoperative swelling,short duration of pain in chil-dren,as well as the shortened hospitalization time,reduced costs,especially good efficacy for joint function recovery.

Objective To evaluate the relationship between relative perfusion rate assessed with power Doppler imaging (PDI) and microvessel density (MVD), as well as expression of vascular endothelial growth factor (VEGF) in bladder carcinoma. Methods The transrectal ultrasound (TRUS) blood flow signal of bladder carcinoma was preoperatively detected with color Doppler imaging in 45 patients, and the relative perfusion rates were obtained for analysis. MVD and the expression of VEGF of excised tumor were assessed immunohistochemically. Results There were correlation between relative perfusion rate and MVD, the expression of VEGF. MVD and the expression of VEGF were related to pathologic grade and the invasiveness of tumor tissues. The expression of VEGF in bladder carcinoma was positively correlated to the tumor interstitial vascular density. Conclusion Combination of PDI and immunohistochemical parameters is useful for evaluating the angiogensis of bladder carcinoma from a different point of view.