Silence information regulator factor 1 (Sirt1) has been demonstrated to be a multi-functional regulator act as a deacetylase.It regulates in critical pathophysiologic pathways,including gene transcription,energy metabolism and senescence.It has been shown that Sirtl regulates the growth and development of myocardial tissue and the formation of blood vessels,also modulates the energy metabolism and oxidative stress of cardiovascular system and so forth.The transcriptional regulator may play a potential role in clinical application and research of cardiovascular diseases.

Objective To clone 3-dehydroquinate synthase cDNA from Echinacea angustifolia and investigate its tissue expression characteristics in various tissues.Methods By using homology cloning and RACE-PCR,the cDNA encoding 3-dehydroquinate synthase was amplified with cDNA library of cultured plantlets as the template.The specificity expression profile in different tissues including roots,stems, leaves,and flowers of E.angustifolia was investigated by semi-quantitative RT-PCR as well.Results The full length cDNA of 3-dehydroquinate synthase(named as EanaroB) had 1 424 bp with an open reading frame encoding 442 amino acids of protein and its EanaroB was around 80%homologous with the sequences of 3-dehydroquinate synthase from other plants.The sequence was reported to the GeneBank and coded as EU293857.The results of semi-quantitative RT-PCR indicated that the expression of EanaroB was detected in different tissues,while only the expression in leaves and flowers reached to a high level. Conclusion The cDNA encoding EU293857 from E.angustifolia is cloned and reported.This work underlays the first step for exploring the pathway of caffeic acid derivatives biosynthesis and improving the content of caffeic acid derivatives including phenylethanoid glycosides.

Cholestasis ; complications ; Consensus ; Humans ; Liver Diseases ; diagnosis ; etiology ; therapy ; Practice Guidelines as Topic

Objective To compare the short-term clinical effect of K-Rod pedicle dynamic fixation system and lumbar fusion for multi-ple segmental lumbar degeneration.Methods Between January 2010 and October 2012,56 patients with segmental lumbar degeneration who were accepted K-Rod pedicle dynamic fixation system and posterior lumbar interbody fusion were retrospectively reviewed.The 56 patients were divided into K-Rod and fusion group,28 patients with K-Rod pedicle dynamic fixation system and 28 cases with traditional lumbar fusion and internal fixation.Clinical assessments were based on comparion of Oswestry disability index score ,visual analogous scale (VAS),im-provement rate of low back pain,postoperative lumbar spine X-ray,average operation time and intraoperative blood loss of two groups.Results All of 56 cases were followed up for 12 to 24 months,the difference of Oswestry disability index score and VAS between two groups were not statistically significant(P >0.05),but the difference of average operation time,blood loss and the average length of stay(ALOS)were statis-tically significant(P <0.05),the K-Rod group was superior to the fusion group.Conclusion In the treatment of multiple segmental lumbar degeneration both K-Rod pedicle dynamic fixation system and traditional lumbar fusion can obtain satisfactory short-term clinical effect,but the K-Rod pedicle dynamic fixation system has advantages of less intraoperative blood loss,shorter operation time,postoperative small effects on lumbar spine mobility.

Objective To review the recent studies on the suppressing function of breast cancer metastasis suppressor 1 (BRMS1) in breast cancer metastasis. Methods The recent literatures on the mechanisms of BRMS1 in the breast cancer that were published in and abroad were reviewed and summarized. Results BRMS1, similar to the other anti-metastasis genes, only suppresses the metastasis of breast cancer cells but has nothing to do with the growth of tumor. BRMS1 could suppress metastasis of tumor cells by reestablishing both the homospecific and the heterospecific gap junctional intercellular comminications (GJIC) and by altering the expressions of relevant metastasis genes in the breast cancer. Conclusion Further studies on BRMS1 may be helpful to understand the metastasis of breast cancer, which may provide a new way for the diagnosis and treatment of breast cancer.

Fibrosis ; Humans ; Inflammation ; MicroRNAs ; Neoplasms

Objective To study the significance of acute pancreatitis AP complications by acid-base distur-bance. Methods The arterial blood gas, serum electrolytes parameter and clinical data of 85 patients were analyzed.Results Acid-base disturbance of different types commonly existed in the early stage of AP patients. There are sig-nificant statistical differences in the indices of PaO2, PaCO2, SaO2 between group MAP and group SAP( P < 0.05 or P < 0.01 ). Conclusion Acid-base disturbance are very common in the AP patients and this-will make the condition worse,they should be treated promptly.

Objective To compare the efficacy of 10-day sequential therapy (including rabeprazole amoxillin clarithromycin tinidazole) and 7-day traditional trigeminy therapy (including rabeprazole amoxillin and clarithromycin) in patients with duodenobulbar ulcer and Hp infection. Methods Ninty-five patients with duodenobulbar ulcer and Hp infection were enrolled into the study and divided into two groups randomly:sequential therapy group and traditional trigeminy therapy group. Patients in the first group received 10-day sequential medications:rabeprazole 10 mg plus amoxillin 1 g for the first 5 days,followed by rabeprazole 10 mg plus clarithromycin 500 mg and tinidazole 500 mg for another 5 days;while in the second group patients received the standard 7-day traditional triple medications:rabeprazole 10 mg plus clarithromycin 500 mg and tinidazole 400 mg. All drugs were given twice daily. All patients received rabeprazole 10 mg daily following the two types of therapys for another four weeks. Hp statuses were assessed by rapid urease test and 14C urea breath test at baseline and 4 weeks after completion of the treatment. Ulcer cicatrization was assessed by gastroscope. Results The eradication rate of Hp infection was significantly higher in sequential group than triple group (89.5%vs. 70. 2%, P ＜ 0. 05 ), but we found no statistically significant difference in the comparison of the ulcer cicatrization rate between sequential group and triple group ( 87.5% vs. 82. 9%, P ＞ 0. 05 ). Conclusion Sequential therapy had a better Hp eradication effect than standard triple therapy, but the eradication rate was very close between the two therapys.

Objective To Study the correlation factors for diabetes pathogenesis of Uygur Nationality in Urumuqi.Methods A cluster sampling was done in 1002 Uygur people aged 25 to 74 years in a part of working units and living area in Urumuqi.All the people received 75g oral glucose tolerance test.Waist and hip circumference,blood pressure,height and body weight were measured.A questionnaire involved in the family history,diet,physical activity and the expenditure on diet was taken.Results(1)63% of the people was male,while 37% was female in our study.The constituent ratio of age was as following:25y～,23.08%;35y～,36.90%;45y～,19.64%;55y～,14.86%and 65y～,5.51%.(2)The prevalence of DM in the study was 14.69%,the abnormality of glucose metabolism was 29.89%.(3)The correlation factors of diabetes were family history of diabetes(OR 0.59),age(OR 0.83),high blood pressure(OR 0.912),waist-to-hip ratio(OR 3.851)and financial spending on diet(OR 5.279) in logistic regression analysis.Conclusions Diabetes-correlated factors are high financial spending on food especially meat products and little cellulose intake,high waist-to-hip ratio.To decrease the prevalence rate of diabetes in Uygur nationality,it is most important to change the diet habit and decrease intra-abdominal fat deposition

Objective To investigate the effects of aerobic exercise training on oxidative stress and ultrastructure of pancreas tissue in diabetic rats and the mechanism of protective effects of aerobic exercise training on pancreatic mitochondria in diabetic rats.Methods Thirty Sprague-Dawley rats were randomly divided into three groups:a normal control group,a diabetic model group and an exercise diabetic group,with 10 rats in each group.Type 2 diabetic rat models were established by intra-abdominal injection of streptozotocin (STZ).The success rate of establishing diabetic rat models was 85.7％.The normal control group and diabetic model group were not given exercise training.Exercise group was trained with 8 weeks of swimming of medium strength,eg.60 min/time,5 d/week. After training for 8 weeks, the activities of superoxide dismutase(SOD), glutathion peroxidase (GPX),nitric oxide synthase (NOS) and the contents of malondialdehyde (MDA) and nitric oxide (NO) in pancreatic mitochondria were observed respectively.The ultrastructures of pancreas tissue in rats were observed under electron microscope.Results After swimming training for 8 weeks,the activities of SOD and GPx in pancreatic mitochondria in exercise diabetic group elevated more obviously than those in diabetic model group ( P ＜ 0.05 ),while the activity of NOS and the contents of MDA and NO reduced obviously (P ＜ 0.05 ).Compared with diabetic model group,the ultrastructures of pancreas tissue in exercise diabetic group improved,and the histomorphology was similar to normal control group.Conclusions Aerobic exercise training can provide protective effects on pancreas in diabetic rats,and can enhance antioxidation ability to eliminate free radicals,improve the ultrastructures of pancreas tissue.