We used (H~3)-prazosin on rabbits to determine the changes of ?_1-adrenoceptor number (Bmax) and affinity (k_D) before and after ischemia, Within 15min of ischemia, we demonstrated that Bmax increased to twfold in ischeamic regions. The increase persisted for 30 min of ischemla and then began to fall at 45 min of ischemia. At the same time, in the nonischemic regions Bmax did not change. The results also showed that ischemia did not alter K_D markedly in both nonischeamic and ischeamic regions.

Objective To explore the differences in immune responses between Cricetulus barabensis and their albino mutant infected with Trichinella spiralis.Methods The physiological parameters of blood , expression levels of IL-2 protein and IL-6 gene in the spleen were analyzed.Results The level of immune cells and cytokines of Cricetulus barabensis was higher than that in the albino mutant .Conclusions Cricetulus barabensis is a suitable model animal for research on long-term latent infection such as infection with Trichinella spiralis.

Two recombinant plasmids pVAX/Sj23 and pVAX/mIL-18 containing Schistosoma japonicum 23 000 membrane protein (Sj23) and murine IL-18 were evaluated for their ability to induce immune responses and to protect against S. japonicum challenge in mice. All animals vaccinated with pVAX/Sj23 alone or plus pVAX/mIL-18 developed specific anti-SWAP (soluble worm antigen preparation) ELISA antibody and splenocyte proliferation response,and co-injection of pVAX/mIL-18 significantly increased the production of IFN-γ and IL-2 compared with pVAX/Sj23 alone, indicating that IL-18 enhances the Th1-dominant immune response. The challenge experiment showed that worm reduction rates in pVAX/Sj23 group compared with control group (pVAX1) was 26.5% and in the pVAX/Sj23 plus pVAX/mIL-18 group was 41.9% ,and the hepatic egg reduction rates were 42.7 and 49.6%,respectively. These results indicated that co-injection of an IL-18 plasmid with Sj23 DNA vaccine efficiently improves the protective effect against S. japonicum infection.

Patients with advanced hepatocellular carcinoma (HCC) often have poor prognosis, and early diagnosis and effective treatment measures can significantly improve the survival rate of patients. At present, alpha-fetoprotein (AFP) is the most widely used serum marker for the diagnosis of HCC in the world; however, no increase in AFP was found in some HCC patients. This article analyzes the application potential of AFP, hepatitis B core-related antigen, liquid biopsy technology, microRNA, long non-coding RNA, and exosomes in the early diagnosis of HCC and reviews related research advances, so as to provide a basis for exploring new methods for the early diagnosis of HCC.

The incurable chronic infection caused by hepatitis B virus (HBV) is the major health burden worldwide. Covalently closed circular DNA (cccDNA) exists in the nucleus of infected cells as a stable minichromosome, and when a new therapy realizes inactivation or eliminates persistent cccDNA in infected hepatocytes, the natural process of chronic infection and long-term antiviral therapy will no longer exist. This article introduces the methods targeting cccDNA, such as gene editing and epigenetic modification, so as to achieve the complete cure of HBV infection.

Objective:To analysis the status of the mental health and related factors of fire fighters in Tianjin Binhai New District, and to provide suggestions for their psychological health protection.Methods:399 fire fighters in Tianjin Binhai New District were selected as study subjects in Jan to April 2019. Depression symptoms were measured by the depression module of the Patient Health Questionnaire (PHQ-9) . The Chinese version of Efrort Reword Imbalance (ERI) Questionnnaire were used to investigate and evaluate their occupational stress. Chi-Square test was used to analysis Categorical data. Binary logistic regression model was used to analysis the ralated factors of depression.Results:Among the 399 fire fighters, 71.1% (280/394) were found high level of depression symptom. The detection rates of depression symptoms in the related influceing factors ERI、station、disease、life pressure、eating habits and sleep disorder occupational stress were difierent ( P<0.05) . Sleep disorder, life pressure and ERI occupational stress were risk factors for depressive symptoms ( OR=1.921, 95% CI=1.002-3.682; OR=2.852, 95% CI=1.561-5.212; OR=2.367, 95% CI=1.163-4.818, P<0.05) . Conclusion:The rate of depression of fire fighters is relatively higher. Government should pay attention to and take measures to improve the psychological condition of fire fighters.

Objective:To analysis the status of the mental health and related factors of fire fighters in Tianjin Binhai New District, and to provide suggestions for their psychological health protection.Methods:399 fire fighters in Tianjin Binhai New District were selected as study subjects in Jan to April 2019. Depression symptoms were measured by the depression module of the Patient Health Questionnaire (PHQ-9) . The Chinese version of Efrort Reword Imbalance (ERI) Questionnnaire were used to investigate and evaluate their occupational stress. Chi-Square test was used to analysis Categorical data. Binary logistic regression model was used to analysis the ralated factors of depression.Results:Among the 399 fire fighters, 71.1% (280/394) were found high level of depression symptom. The detection rates of depression symptoms in the related influceing factors ERI、station、disease、life pressure、eating habits and sleep disorder occupational stress were difierent ( P<0.05) . Sleep disorder, life pressure and ERI occupational stress were risk factors for depressive symptoms ( OR=1.921, 95% CI=1.002-3.682; OR=2.852, 95% CI=1.561-5.212; OR=2.367, 95% CI=1.163-4.818, P<0.05) . Conclusion:The rate of depression of fire fighters is relatively higher. Government should pay attention to and take measures to improve the psychological condition of fire fighters.

Objective To investigate the impact of a peer support model on the mental health of caregivers and the perceived social support and psychiatric symptoms of the mental disorder patients under their care.Methods Patients with mental disorders undergoing long-term community-based rehabilitation and their primary caregivers were recruited for this study.A total of 44 pairs of eligible patients and caregivers were selected based on a 1∶1 matching ratio.Systematic peer support activi-ties were conducted exclusively for the caregivers.The General Health Questionnaire(GHQ)and the Symptom Checklist-90(SCL-90)were administered before and after the intervention to assess the mental health status of caregivers.The Perceived Social Support Scale(PSSS)and the Brief Psychiat-ric Rating Scale(BPRS)were employed to evaluate the patients'perceived social support and psychi-atric conditions before and after the intervention.Results A total of 44 valid questionnaires from car-egivers and 42 from patients were collected.The GHQ score and the total scores,the number of positive item,positive total scores,and positive mean scores of and SCL-90 of caregivers were significantly low-er after the intervention compared to pre-intervention(P＜0.05 or P＜0.001).For patients,the fami-ly support score,extracurricular support score,and total score of the PSSS were significantly higher post-intervention compared to pre-intervention(P＜0.05 or P＜0.001).Compared to pre-intervention,patients'scores on anxiety and depression,lack of energy,thought disturbance,activation,hostility and suspicion,and the total score on the BPRS were significantly lower after the caregiver peer sup-port intervention(P＜0.001).Conclusion The peer support model contributes to improving the mental health of caregivers for patients with mental disorders and enhancing the patients'psychiatric conditions.

Objective To investigate the impact of a peer support model on the mental health of caregivers and the perceived social support and psychiatric symptoms of the mental disorder patients under their care.Methods Patients with mental disorders undergoing long-term community-based rehabilitation and their primary caregivers were recruited for this study.A total of 44 pairs of eligible patients and caregivers were selected based on a 1∶1 matching ratio.Systematic peer support activi-ties were conducted exclusively for the caregivers.The General Health Questionnaire(GHQ)and the Symptom Checklist-90(SCL-90)were administered before and after the intervention to assess the mental health status of caregivers.The Perceived Social Support Scale(PSSS)and the Brief Psychiat-ric Rating Scale(BPRS)were employed to evaluate the patients'perceived social support and psychi-atric conditions before and after the intervention.Results A total of 44 valid questionnaires from car-egivers and 42 from patients were collected.The GHQ score and the total scores,the number of positive item,positive total scores,and positive mean scores of and SCL-90 of caregivers were significantly low-er after the intervention compared to pre-intervention(P＜0.05 or P＜0.001).For patients,the fami-ly support score,extracurricular support score,and total score of the PSSS were significantly higher post-intervention compared to pre-intervention(P＜0.05 or P＜0.001).Compared to pre-intervention,patients'scores on anxiety and depression,lack of energy,thought disturbance,activation,hostility and suspicion,and the total score on the BPRS were significantly lower after the caregiver peer sup-port intervention(P＜0.001).Conclusion The peer support model contributes to improving the mental health of caregivers for patients with mental disorders and enhancing the patients'psychiatric conditions.

Objective To establish a scientific training program that takes into account both anaerobic and aerobic training for pilots,and to explore the appropriate ratio of aerobic and anaerobic training.Methods According to the physical examination standards for pilots,a total of 16 healthy subjects aged 18-24 were selected from two batches.The two batches of subjects were trained with different aerobic and anaerobic ratios.Training period was 3 months.The changes in cardiopulmonary function of the subjects before and after training were evaluated using the cardiopulmonary function exercise testing system(CPET),and the changes in anaerobic capacity were evaluated using changes in strength as an indicator.Results After training,the weight load of the subjects in the two training programs,including barbell squats,leg flexion and hard pull,and barbell under 10RM and 3RM,was significantly increased(P<0.001),and there was no statistically significant difference in anaerobic strength growth between the two groups.The results of CPET showed that the maximum load,maximum heart rate,and respiratory quotient in the two groups were significantly increased after than before the training(P<0.01).The maximum load(Experiment group 1:29.12±19.69,Experiment group 2:72.00±46.24)and respiratory quotient(Experiment grouop 1:0.11±0.09,Experiment group 2:0.28±0.16)of the subjects in experiment group 2 before and after training were greater than those in experiment group 1.The difference was statistically significant(P<0.05).Conclusion The anaerobic and aerobic capacities of the subjects in the experiment group 2 are effectively improved,indicating that ratio of aerobic and anaerobic of the training scheme is better.