Objective To design reasonable informatization model of nursing human resource allocation and to apply it to clinical nursing so as to make nursing human resource allocation scientific and reasonable.Methods The time table was designed according to the program of direct nursing and indirect nursing. It was tested in five surgical departments between January 19th 2016 and January 23rd 2016 and five internal medicine between January 24th 2016 and January 28th 2016. The situation before and after allocation were compared with the questionnaires of quality of nursing, patients' satisfaction and nurses' satisfaction. Results The nursing human resource allocation model built with information technology could export the hours of direct nursing in real time and configure nursing staff reasonably in line with the hours of nursing. The quality of nursing, patients' satisfaction and nurses' job satisfaction after the reasonable allocation were higher than those before allocation with statistically significant differences (t=-29.646, -5.783, -2.369;P<0.05). Conclusions The nursing human resource allocation designed by the research is with real time. Besides, it can improve the quality of nursing, patients' satisfaction and nurses' job satisfaction.