Objective To analyze the clinical features of severe pneumonia complicated with congenital heart disease (CHD) . Methods The clinical data of 270 children patients with severe pneumonia complicated with CHD (CHD group) were collected to analyze the relative medical history ,etiology ,bacterial drug resistance and clinical outcomes .Moreover ,636 age-matched children pa-tients with pure severe pneumonia were selected as the control group .Results In the CHD group ,250 cases were infants and 35 ca-ses suffered from repeated pneumonia .Compared with the control group ,the CHD group was earlier in onset and more prone to re-peated pneumonia .Among 270 cases ,totally 220 cases were checked out the pathogens ,but no statistically significant difference in the detection rates of pathogens between the CHD group and the control group was found (P>0 .05);179 cases were infected by bacteria ,in which 126 cases were Gram-negative bacteria ,the positive rate of bacterial infection and the detection rate of Gram-nega-tive bacterial were relatively higher than those in the control group ,the difference was statistically significant (P<0 .05);95 cases were infected by virus ,with respiratory syncytial virus being the most common pathogen ,30 cases were infected by fungi ,with Can-dida albicans being predominant ,the infection rates of virus and fungus had no statistical difference between two groups (P>0 .05);in the drug sensitive test ,the detection rate of extended spectrum beta lactamase (ESBLs)-producing positive bacteria in the CHD group was higher than that in the control group ,the difference was statistically significant (P<0 .05) .Main Gram-negative bacteria Pneumonia klebsiella pneumonia subspecies ,Escherichia coli and haemophilus inf luenzae were highly sensitive to imipenem ,mero-penem ,levofloxacin and ciprofloxacin ;main Gram-positive bacteria Staphylococcus aureus and streptococcus pneumoniae were highly sensitive to vancomycin and gentamicin .Especially ,pneumonia klebsiella pneumonia subspecies ,Escherichia coli and haemophilus influenzaewere100% resistanttopenicillin.TheaveragelengthofhospitalstayintheCHDgroupwas (20.9±12.5)d,207cases developed the respiratory failure ,88 cases appeared the heart failure and 205 cases were effective in treatment ;compared with the control group ,the CHD group had a longer length of hospital stay ,higher occurrence rate of heart and respiratory failure and lower treatment effective rate (P<0 .05) .Conclusion Because of the earlier onset ,more prone to repeated pneumonia ,more sensitive to bacterial infection ,esp .Gram-negative bacteria ,more ESBLs production ,longer hospital stay and more prone to complications ,com-pared with the control group ,children with severe pneumonia complicated with CHD require active treatment .

Objective To investigate the effect of cold underwear on acute radioactive dermatitis in the cervical cancer patients undergoing radiotherapy.Methods Ninety-seven patients with stage Ⅱb and above Ⅱb cervical cancer receiving radiotherapy were divided into the control group (n =48) and the experiment group (n =49) according to the random digit table.In the control group,Orgotein was sprayed on the local skins and the experiment group was treated with wearing cold underwear for 20 minutes in addition to local spraying of Orgotein.The two groups were compared in terms of dermatitis on the early stage,middle stage and final stage.Result On the early stage there was no statistical significant difference between two groups on dermatitis (P＞0.05),but the dermatitis in the experiment group was statistically less than that in the control group at the middle stage and final stage (P＜0.01).Conclusions The cold underwear for the cervical cancer patients undergoing radiotherapy can effectively prevent dermatitis or reduce its severity.It is designed suitable for patients prom anatomical perspective and simple for application.

Objective: To study the effects of pharmaco-serum from rabbits administrated intragastrically with Fuzheng Jiedu decoction(FJD) on injured human bronchial epithelial(16HBE) cells exposed to nickel sulfate(NiSO4).Methods: Cultured 16HBE cells were treated with both NiSO4 and different doses of FJD pharmaco-serum in vitro.The detections of cell activities and viabilities were carried out.Meanwhile,activities of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) as well as maleic dialdehyde(MDA) contents were measured.Results: FJD pharmaco-serum could decrease mortalities and increase viabilities of 16HBE cells exposed to NiSO4.In cells co-treated with NiSO4 and FJD pharmaco-serum,the content of MDA was decreased,while GSH-Px and SOD activities were increased at the same time.High dose of FJD pharmaco-serum had the most dramatic effect.Conclusion: This study suggested that FJD could antagonize NiSO4-induced toxicity,which may be involved in its antioxidant function.

Objective To study the multicomponent in vivo dynamic process in Chuanxiong Rhizoma;To elaborate in vivo metabolic profiling. Methods HPLC was used to establish the fingerprint of aqueous extract of Chuanxiong Rhizoma, and multicomponent changes were detected at the same time. Closed-loop intestine method was used to study the multicomponent changes of oral administration of Chuanxiong Rhizoma after stomach-intestine-liver process. Results Totally 17 components were detected in the fingerprint of aqueous extract of Chuanxiong Rhizoma and they were basically stable in the digestive juice. For in vivo metabolism, 4 components were metabolized by intestinal flora;3 components were metabolized by liver;2 new components were the metabolites of intestinal flora;1 component was the metabolite of liver. Conclusion Multicomponent sequential metabolism and closed-loop intestine method were used to clarify that multicomponent metabolic profiling was feasible, and it could provide experimental basis for the metabolism of traditional Chinese medicine.

Objective To investigate the early diagnosis and treatment of primary gastrointestinal malignant lymphoma( PGIML). Methods The clinical materials of 33 cases with PGIML from 1997 to 2005 were retrospectively studied. Results The diagnosis rate of gastroenteroscopy was 44. 4% . The most common immune phenotype was B-cell lineage (90. 9% ). Of the 33 PGIML patients, 30 were treated with operation, 2 with H. poli elimination and 1 gave up treatment. Among the operation cases and operation plus chemotherapy cases 19/21 cases (90. 5% )and 6/9 cases (66. 7% ) survived more than 2 years respectively, the other 6 cases died. Conclusion It is hard to diagnose PGIML in early stage. The effectiveness of surgery on treating PGIML should be further confirmed by profound prospective randomized trials.

Objective: To study the role of vascular endothelial growth factor (VEGF) during angiogenesis in oral squamous cell carcinoma (SCC).Methods:Surgical specimens from 45 patients with oral SCC were examined for VEGF expression and microvessel density(MVD) by immunocytochemical staining. Anti-VEGF polyclonal antibody was used to determine VEGF expression and anti-CD 34 monoclonal antibody was used to determine MVD. Results:①VEGF protein was mainly found in cytoplasm of cancer cells and in few endothelial cells. Stained microvessels were mainly distributed in the regions of the connective tissue near the cancer nest;②there was a close positive correlation between MVD and VEGF expression;③SCC with high MVD or high VEGF level was mostly found with metastasis in cervical lymph node (P

Objective:o prepare Danshensu liposomes and investigate drug release characteristics in vitro. Methods: Danshensu liposomes were prepared by a reverse-phase evaporation method. The encapsulation efficiency was used as the index, an orthogonal test was adopted to investigate the effect of concentration of soybean lecithin, ratio of lipid-Danshensu and pH value of solution on the preparation procedure of Danshensu liposomes. The particle size of the liposomes was also investigated by a transmission electron micro-scope ( TEM) . The concentration of Danshensu was determined by HPLC, and the difference of release characteristics in Danshensu li-posomes and Danshensu solution was measured by a dialysis method. Results:The optimum preparation technology was as follows:the concentration of soybean lecithin was 40 mg·ml-1 ,the ratio of drug-lipid was 1: 10,and the pH value of solution was 6. 6. The mor-phology of the prepared liposomes showed spheric structure with uniform diameter, and the average particle size was ( 174 ± 36 ) nm and the encapsulation efficiency was 38. 9%. The linear range of Danshensu was 2. 0-20. 0 mg·L-1(r=0. 9984). The drug release of liposomes in vitro was slower than that of free Danshensu solution in 24 h. Conclusion:Danshensu liposomes with fine morphology have sustained release property.

Objective To explore effects of water extract of radix salviae miltiorrhizae on renal injury in rats exposed to cadmium and its possible mechanism.Methods Twenty male and female healthy Wistar rats,respectively,at age of 6 weeks were randomly divided into blank group (n =8),and model group (n =32) after adaptive feeding for 1 week,given intraperitoneal injection of saline for 5 mL/(kg · d) and intraperitoneal injection of cadmium chloride solution for 2.8 mg/(kg · d).Continuously building for 4 weeks,the general situation of rats was observed,and the rat kidney function and kidney tissue pathological changes were detected.After successful molding,the model group was randomly divided into medicine group,negative and positive and blank control groups.The medicine group was given Salvia miltiorrhiza water extract of high dose of 2.7g/(kg · d),Danshen aqueous extract of low dose 1.35 g/(kg · d) by gavage,positive control group was given sodium selenite for 0.05 mg/kg · d by gavage,the negative and blank control groups were given the same mount of saline for 2.7 mL/(kg · d).After 10 weeks of treatment,serun and urine was collected,and kidney tissue was to be detected.Results Salvia miltiorrhiza water extraction liquid of low and high dose group had less 24 h urine volume than negative control group,and serum superoxide dismutases (SOD) increased significantly,Malondialdehyde (MDA) decreased significantly,total antioxidant capacity (T-AOC) increased significantly,blood urea nitrogen,urinary beta β2-MG content and serum creatinine levels was decreased.Pathological section revealed that renal proximal convoluted tubules epithelial cells was turbid,swelling,degeneration and necrosis in the negative control group.Salvia miltiorrhiza water extraction liquid of low and high dose group had lower serum cortical cadmium content than the negative control group,Salvia miltiorrhiza water extraction liquid of low and high dose group had lower apoptosis rate than the negative control group.Conclusion The salvia miltiorrhiza water solution can accelerate the metabolism of cadmium,and effectively interfere with cadmium induced renal injury,delay chronic renal failure,and protect the kidney.

Objective To explore effects of water extract of radix salviae miltiorrhizae on renal injury in rats exposed to cadmium and its possible mechanism.Methods Twenty male and female healthy Wistar rats,respectively,at age of 6 weeks were randomly divided into blank group (n =8),and model group (n =32) after adaptive feeding for 1 week,given intraperitoneal injection of saline for 5 mL/(kg · d) and intraperitoneal injection of cadmium chloride solution for 2.8 mg/(kg · d).Continuously building for 4 weeks,the general situation of rats was observed,and the rat kidney function and kidney tissue pathological changes were detected.After successful molding,the model group was randomly divided into medicine group,negative and positive and blank control groups.The medicine group was given Salvia miltiorrhiza water extract of high dose of 2.7g/(kg · d),Danshen aqueous extract of low dose 1.35 g/(kg · d) by gavage,positive control group was given sodium selenite for 0.05 mg/kg · d by gavage,the negative and blank control groups were given the same mount of saline for 2.7 mL/(kg · d).After 10 weeks of treatment,serun and urine was collected,and kidney tissue was to be detected.Results Salvia miltiorrhiza water extraction liquid of low and high dose group had less 24 h urine volume than negative control group,and serum superoxide dismutases (SOD) increased significantly,Malondialdehyde (MDA) decreased significantly,total antioxidant capacity (T-AOC) increased significantly,blood urea nitrogen,urinary beta β2-MG content and serum creatinine levels was decreased.Pathological section revealed that renal proximal convoluted tubules epithelial cells was turbid,swelling,degeneration and necrosis in the negative control group.Salvia miltiorrhiza water extraction liquid of low and high dose group had lower serum cortical cadmium content than the negative control group,Salvia miltiorrhiza water extraction liquid of low and high dose group had lower apoptosis rate than the negative control group.Conclusion The salvia miltiorrhiza water solution can accelerate the metabolism of cadmium,and effectively interfere with cadmium induced renal injury,delay chronic renal failure,and protect the kidney.

ObjectiveTo investigate the application value of negative pressure sucking technique in the elderly after nasal septal reconstruction. MethodsForty elderly patients after nasal septal reconstruction were divided into two groups randomly, with twenty patients in each. The cases in observing group received negative pressure sucking technique while those in controlling group received nasal packing with vaseline gauze following the surgery. The amount of bleeding of the first 24 hours after operation, the comfort degrees evaluated by using visual analogue scale (VAS),hematoma of nasal septum, edema in nasal cavity and duration of hospitalization after operation were compared between the two groups. ResultsThe amount of bleeding of the first 24 hours afteroperation was more in observing group than in controlling group[(14.2±8.1)ml vs. (7.2±4.1)ml, P<0.05]. The VAS score and the number of cases who felt discomfortable moderately or severely were significantly less in observing group than in controlling group at 6 h, 12 h and 24 h after operation(all P<0.05). The nasal cavity gap were significantly wider in observing group at the 3rd day after operation(P=0.001), and the duration of hospitalization after operation was significantly shorter in observing group [(4.5±0.9) d] than in controlling group [(5.42±0.9) d](P=0.042)Conclusions Negative pressure sucking technique applied in the elderly after nasal septal reconstruction can significantly relieve the distress of patients, reduce the edema in nasal cavity and shorten the duration of hospitalization, but does not increase the risk of hemorrhage.