Objective To compare the cellular characteristics of the nucleus pulposus(NP) cells of young and adult rabbits.Methods The structure and arrangement of the NP cells from young and adult rabbits were investigated on both tissue and ultrastructure level by confocal laser scanning microscope and transmission electron microscope. Results The NP cells from the young rabbit discs,which were round and contained numerous large inclusion bodies,often formed clusters with a diameter of(266?167)??m and a density of(8.0?2.4) per high power(?40);The NP cells from the adult rabbit discs were round or oval shape and had no large size inclusion bodies,formed less clusters or were singly diffusedly distributed,with the diameter of clusters to be(94?42)??m(single cell excluded) and density to be(14.9?4.3) per high power(?40).Conclusion There were remarkable morphological differences in the cell architecture of nuclei pulposi between the young and adult rebbits.These features of the aging and degenerating disc contribute to decreased biological function.

Objective To study value of ultrasonography in early diabetic nephropathy.Methods 60 patients with early diabetic nephropathy were enrolled into the observation group and 30 healthy people were enrolled into the control group.Ultrasound was used to measure all renal artery segment that peak systolic velocity( PSV) ,end-diastolic flow velocity( EDV) ,and calculate the pulsatility index( PI) and resistance index( RI) .Serum high sensitivity C reactive protein( hs-CRP) was measured.Results In the control group,renal artery,segmental artery and the artery between the leaves of PSV were (66.1 ±12.1) cm/s,(44.3 ±11.3) cm/s,(27.9 ±9.1) cm/s.In the observation group,renal artery,segmental artery and the artery between the leaves of PSV were (53.6 ±9.2) cm/s,(38.1 ± 8.2)cm/s,(21.1 ±6.2) cm/s.Renal artery,segmental artery,interlobar artery PSV in the observation group were lower than those of the control group(t =3.964,4.016,2.726,all P<0.05).In the control group,renal artery, segmental artery and the artery between the leaves of EDV were (25.1 ±6.5)cm/s,(18.6 ±7.1)cm/s,(11.2 ± 5.6)cm/s and (11.2 ±5.4) cm/s,(9.6 ±4.1) cm/s,(6.2 ±3.5) cm/s in the observation group.Renal artery, segmental artery,interlobar artery EDV in the observation group were lower than those of the control group( t=4.972, 6.014,5.112,all P<0.05).Renal artery,segmental artery and the artery between the leaves of RI in the control group were (0.51 ±0.08),(0.42 ±0.06),(0.41 ±0.07) and (0.59 ±0.09),(0.59 ±0.07),(0.53 ±0.05) in the observation group.Renal artery,segmental artery,interlobar artery RI in the observation group were higher than those of the control group(t =2.061,2.916,3.142,all P <0.05).Renal artery,segmental artery and the artery between the leaves of PI in the control group were (0.82 ±0.16),(0.71 ±0.12),(0.62 ±0.09) and (0.91 ± 0.18),(0.82 ±0.11),(0.75 ±0.06) in the observation group.Renal artery,segmental artery,interlobar artery PI in the observation group were higher than those of the control group(t =3.716,2.612,2.468,all P <0.05). Conclusion Early diabetic nephropathy in renal blood flow is abnormal,ultrasonography can determine the severity.

Taking the characteristics of Indian students into account,teaching quality can be improved by these means:thorough preparation before class,various application for teaching,mutual and interactive communication and inspiration.

Objective To study the possibility of the exposed therapy of the male's perineum after operation.Methods 450 male patients with perineum surgical operation were chosen,250 among whom were adopted the exposed therapy ,200 were adopted the traditional tie-up therapy after the operation.Calculation with the statistic software SPSS 17.0 were adopted to observe the wound germ infection and heal circumstance,to statisfy the first grade heal rate and the wound germ infection rate.x2 examinations were used, too.Results The rate of the first grades healing of the exposed therapy group was obviously higher than the tie-up therapy.There was obvious difference.(x2 = 12.2,P <0.01).The rate of the germ infection of the exposed therapy set was closed to that of the tie-up therapy without obvious difference(x2 =0.06 ,P >0.05 ).Conclusion The rate of the wound germ infection would not increase if adopted the exposed therapy of the male's perineum after operation and the rate of the wound first grade heals leads to an obvious exaltation.

On the basis of the bioluminescent detection technology of the lux gene being a kind of new and progressing environmental detection technology,in this paper,the constitution,function,expression and regulation of the lux gene were introduced,moreover,the recent advance of its application in the study of environmental detection was reviewed

Objective To explore morphologic characterizatics of cellular processes from adult human nucleus pulposus cells. Methods The nucleus pulposus of adult human intervertebral disc were obtained from 8 patients(Thompson's grade Ⅰ-Ⅱ) and then the tissues specimens were carried out by frozen section and electron microscopic section as well as cell isolation and cultured,processes of nucleus pulposus cells were examined using light microscopy,laser scanning confocal microscopy and transmission electron microscopy. Results When examined at both the confocal and electron microscope level,all the cells possessed the processes and adjacent nucleus pulposus cells processes possessed a gap junction.The elongated and round cells were examined when NP cells became monolayer in vitro.The rate of elongated cells to round cells was 2.3 to 1.The elongated cells protruded along with the long axis of cell body without second processes.Dendritic processes of round cells protruded to all directions from the cell body with multiple-level processes.Conclusion Processes are one of the morphologic characteristics of intervertebral disc cells.The research on processes functions could be helpful to understand pathomechanism of intervertebral disc degradation.

Objective To investigate the protective effect and mechanism of naloxone preconditioning ( NP) for rats with cerebral hemorrhage. Methods Totally 30 healthy male Sprague Dawley ( SD) rats were randomly separated into Sham operatopm group,intracerebral hemorrhage ( ICH) and NP treatment groups ( n =10) . Before the establishment of rat cerebral hemorrhage model,rats in the NP group were given an intraperitoneal injection of naloxone hydrochloride ( 2. 0 mg/kg) ,and those of ICH group received an injection of normal saline at same volume. Then,the ICH model was made by injecting 0. 4 IU collagen Ⅶ into caudate nucleus while. The brain tissue samples around cerebral hemorrhagic focus after ICH were collected. RT-PCR and Western blotting were used to detect the transcriptional level and protein level of aquaporin protein 4 ( AQP4) in the brain tissue,respectively. Meanwhile,immunohistochemistry was employed to determine the expression of matrix metalloproteinase 9 ( MMP-9) in the rat brain tissue. Results Brain water content in ICH group was larger than that in Sham group ( P

Objective To evaluate the clinical value of humeral antegrad e interlocking nails. Method 21 patients with humeral shaft fracture were treate d with open reduction and internal fixation with antegrade interlocking nail. Re sult The patients were followed up for 3 to 20 months (mean 14.6months). All pat ients acquired bone healing, and only one case had temporary injury of radius ne rve. Conclusion The humeral antegrade interlocking nail is an effective method t o treat humeral shaft fracture, with advantages of good elbow function and less complications of infection and injury of radius nerve.

Objective To understand the expression change of SFRP2 in human cervical cancer tissue and to investigate the effect of SFRP2 on cervical cancer cell proliferation.Methods The expression of SFRP2 in cervical cancer tissue was detected by using Western blot and qRT-PCR;the SFRP overexpressed human cervical cancer line was constructed by using lentivirus,the effect of SFRP2 on the proliferation of human cervical cancer cell line was analyzed by CCK-8 and plate cloning.The effect of SFRP2 on the expression of WNT pathway related proteins and genes in human cervical cancer cell was detected by Western Bolt and qRTPCR.Results Compared with paracancerous tissue,SFRP2 was lowly expressed in human cervical cancer tissue;overexpressed SFRP2 cervical cancer cell proliferation was inhibited;SFRP2 inhibiting cellular proliferation was occurred via WNT signal pathway.Conclusion The role of SFRP2 as a candidate gene for cervical cancer remains to be deeply studied.

Objective To explore the antitumor effect of interleukin 21(IL-21) after transfected into in HeLa cells in severe combined immunodeficiency(SCID) mice or human peripheral blood lymphocytes-SCID(hu-PBL-SCID) mice.Methods pcDNA4/HisMax-IL-21 was transfected into human cervical cancer cell line HeLa to produce HeLa-IL-21 cells.Totally 48 SCID mice were randomly and equally divided into SCID group(0.2 ml PBS,i.p.) and hu-PBL-SCID group(0.2 ml 2?107/ml hu-PBL,i.p.).Then every group was further divided into 3 subgroups(n=6) to receive a subcutemeous implantation of HeLa,HeLa-vector or HeLa-IL-21 cells,and the rest 6 mice were intraperitoneally injected with anti-asialo GM1 50 mg/animal 1 d before and 4 d after HeLa-IL-21 cells transplantation for NK cell depletion.The growth of the tumor mass was observed.Flow cytometry was used to detect CD8-and DX5-positive cells in hu-PBL from hu-PBL-SCID mice,and spleen NK cells from SCID mice or hu-PBL-SCID mice.The secretion of IFN-? and cytotoxicity of splenic cells from HeLa/HeLa-IL-21-bearing SCID mice or hu-PBL-SCID mice were detected with double sandwich ELISA assay and LDH assay respectively.Results Growth of HeLa-IL-21 tumors was significantly suppressed compared with that of HeLa cells,with a slow development and smaller volume.But this growth suppression was not observed in NK cell depletion groups,with SCID group more severe than hu-PBL-SCID group.hu-PBL-SCID mice bearing HeLa-IL-21 cells(16.55?4.53)had a higher toxicity in spleen cells than those SCID mice bearing HeLa-IL-21 cells(8.32?2.12) or HeLa cells(3.42?1.56)(P