Objective To study the changes of expression levels of vascular endothelial growth factor(VEGF) and its receptors(FLT-1,FLK-1) mRNA in equal area of penumbra in diabetes rats with cerebral infarction(DMCI).Methods Middle cerebral artery occlusion(MACO) model was made in diabetes rats.The expression levels of VEGF and FLT-1,FLK-1 mRNA in equal area of penumbra were measured with situ hybridization in different times after MACO(1 h,3 h,6 h,12 h,24 h).The difference was compared between DMCI group and no diabetes cerebral infarction(NDMCI) group.Results The expression level of VEGF mRNA was lower in DMCI group than that of NDMCI group at every time point(P

Objective: To get the information about freshmen's mental health and it relationship with sex, city and countryside difference, and to provide the reference for effective mental health education and consultation. Methods: The situation of 1351 freshmen's mental health was surveyed by using UPI. Results: ①11.91% of the freshmen had serious mental derangement, 26.15% of them had a little psychological problem. ②The distinct difference existed not only between male and female, but also between city and countryside. The mental health of male students surpassed the female students, the city students surpassed the countryside students. Conclusion: The situation of medical freshmen's mental health should not be neglected, and its influence factors are various. Mental health education and consultation should be pointed to the students.

OBJECTIVE: To evaluate application of antibiotics and non-steroidal anti-inflammatory drugs in 3 hospitals of Guang’an and find out the reason of unreasonable use of drug. METHODS: 120,000 prescriptions were randomly collected from inpatient and outpatient departments of 3 hospitals from Jan. 2005 to Jan. 2008, which included 20 000 pediatrics prescriptions. The DDDs and DUI of antibiotics and non-steroidal anti-inflammatory drugs were analyzed statistically. RESULTS: The DUI of 10 kinds of antibiotics and 3 kinds of non-steroidal anti-inflammatory drugs were above 1, which indicated that drug abuse occurred. CONCLUSION: Physicians’ prescribing behavior lack medical rationality and the situation of drug feeding hospital is still critical in primary hospitals.

Objective] To analyse the academic thought of Professor Li Guixian, the famous veteran doctor of TCM and academic experience instructor of treating ulcerative colitis. [Methods] In-depth analyse the pathogenesis of ulcerative colitis, explore the academic connotation of Professor Li Guixian treatment of ulcerative colitis based on traditional Chinese medicine theory, according to the ancient classics. [Results] Professor Li Guixian puts forward the viewpoint that pathogenesis in the process of evolution of ulcerative colitis has a great relationship with the fundamental of spleen and stomach, symptoms of damp and heat-toxin, the outline of Qi and blood ,not only in line with the characteristics of the occurrence and prognosis of the disease but also has its theoretical basis ,combined with her many years of clinical experience. [Conclusion] The way of “invigorating spleen to help transport and transform the essence of water and grain, regulating the function of qi and blood, removing the muddle of damp and heat-toxin”of treating ulcerative colitis has clinical significance.

Objective To observe the clinical efficacy of Santeng Tongbi pills on rheumatoid arthritis (RA),evaluate the safety and explore its mechanism. Methods Double-blind and double dummy design method was used. 107 cases of RA were randomly divided into Santeng Tongbi pills group (the treatment group,53 cases) and Zhengqing Fengtongning group (the control group,54 cases). The Ritchie index,health assessment questionnaire (HAQ),arthritis influenced index (AIMS),patinents' assessment to pain (VAS form),acute phase reactant,Chinese medical pattern grade,the hemorheology state and so on were retrospectively analyzed. Results There was significantly difference between RA patients and healthy people on the hemorheology state (P

BACKGROUND: Hepatic fibrosis is a reversible disease, interfering in course of disease promptly can decrease hepatic cirrhosis and fatal complication. Hepatic stellate cells (HSCs) is a key factor in pathogenesy of hepatic fibrosis.OBJECTIVE: To investigate the effects of Shugan granule on HSCs activation and trans-membrane signal transduction stimulated by transforming growth factor beta 1 (TGF-β1) in rats, and to explore the anti-fibrosis mechanism.DESIGN, TIME AND SETTING: Controlled observational trials based on cytology were performed in the Central Laboratory of Molecules, Luzhou Medical College between June 2008 and February 2009. MATERIALS: HSC-T6 cell line was purchased from Institute of Liver Diseases, Shanghai University of Traditional Chinese Medicine, its phenotype was the activated hepatic stellate cells. Shugan granule was offered by Drug Institute, Affiliated Hospital of Luzhou Medical College at a Batch No. 20071120.METHODS: The influence of different concentrations (0.01, 0.02, 0.04, 0.08 g/L) of Shugan granule on HSCs proliferation was determined by MTT. 0.01 g/L was defined as the dose of Shugan granule contributing no influence on cell proliferation. HSCs were cultured in a culture plate and then divided into 4 groups: control group without management, TGF-β1 group with 5 μg/L TGF-β1 solution in culture medium, Shugan granule group with 0.01 g/L Shugan granule in a culture medium, and TGF-β1 + Shugan granule group with 5 μg/L TGF-β1 solution and 0.01 g/L Shugan granule in culture medium. MAIN OUTCOME MEASURES: Morphological features of HSCs were detected by microscopic. Immunohistochemical method was used to detect the expression of Smad3 and Smad7 in HSCs. RT-PCR was applied to observe the HSCs activation and trans-membrane signal transduction. RESULTS: ①The cell morphology of TGF-β1 group was similar with that in the control group, and the extension was more obvious. In the TGF-β1 + Shugan granule group, the cell morphology was close to that in TGF-β1 group. There was no karyopyknosis or apoptosis observed in each group. ②Immunohistochemical method showed the expression of Smad3 and Smad7 in control groups were increased. TGF-β1 could slightly increase the expression of Smad3 and Smad7 (P < 0.05), while Shugan granule group and TGF-β1 + Shugan granule group increased the expression of Smad7 significantly, accounting for 1.99 times compared with control group (P < 0.01). ③RT-PCR result showed that Shugan granule could increase the expression of Smad7 (P < 0.05), but the expression of Smad3 was not regulated. 5 μg/L TGF-β1 could up-regulate the expression of Smad3 and Smad7 (P < 0.05). In the TGF-β1 + Shugan granule group, Smad7 expression was increased by 101% (P < 0.05), but Smad3 transcriptional level was not changed(P > 0.05). CONCLUSION: ①TGF-β can stimulate the gene expression of Smad3 and Smad7, it also obtain a balance of feedback regulation mechanism between R-Smads and I-Smads. ②Shugan granule may prevent and cure hepatic fibrosis through decreasing the proliferation of HSCs in a dose-dependent manner. ③Shugan granule can inhibit the TGF-β-Smad signaling pathway through increasing the expression of Smad7.

Objective To improve the understanding of osteoarticular pain presented as rheumatic syndromes of cancers and malignant skeleton metastasis associated. Methods To analyse the clinical data of 32 patients initially manifested as osteoarticular pain. Results Forteen patients of 32 cases was diagnosed as cancer associated rheumatic syndromes, characterized by gradually deteriorated chronic recurrent vague pain. The effect of non-steroid anti-inflammatory drugs (NSAIDs) and glucocorticoid was not satisfactory. Pain subsided after removal of carcinous mass. Eighteen patients had malignant skeleton metastasis, vague pain at onset, but gradually progressed to be, severe persistent pain. NSAIDs and glucocorticoid were ineffective. Narcotic analgesics could produce short time relief. The nature and distribution of tumour were comparatively extensive. The clinical misdiagnosis rate was high. Conclusion Clinical doctors often neglect the coexistence of tumor because they are not familiar with the relation between osteoarticular pain and tumor. So patients who is over sixty and is not sensitive to commonly used analgesics and glucocorticoid with osteoarticular muscular pain should be considered the possibility of malignant tumor after excluding diffuse connective tissue disease.

Objective To investigate the role of nursing intervention in the prevention of premature rupture of the membrane infection. Methods 131 patients with premature rupture of the membrane were divided into the control group(65 cases) and the experimental group(66 cases), they adopted routine nursing method and nursing intervention respectively. The duration of pregnancy, fetal condition and laboratory tests for infection were compared between two groups. Results No significant differences were found between the two groups in terms of age, duration of pregnancy, newborn weight. Significant differences were observed in Apgar score, newborn death between two groups. Conclusions Monitoring for signs of premature rupture of the membrane such as infection and laboratory tests are important for the prevention of infeclion, if infection occurs, delivery may be necessary to prevent further complications.

Objective:To explore distinguishing value of serum aldosterone level for different types of atrial fibrilla-tion (AF) .Methods:A total of 153 AF patients were selected ,including 99 patients with paroxysmal AF (paroxys-mal AF group) and 54 patients with persistent AF (persistent AF group) .Serum aldosterone concentration was measured ,and its correlation with two types of AF was analyzed .Results:Compared with patients with paroxysmal AF ,there was significant rise in serum aldosterone level [ (234.3 ± 69.6) pg/ml vs .(302.7 ± 78.3) pg/ml , P<0.01] in patients with persistent AF .Correlation analysis indicated that aldosterone level was positively correlated with atrial diameter and left ventricular ejection fraction (r=0.896 ,0.831 ,P<0.01 both) .Conclusion:Serum al-dosterone level is helpful to distinguishing paroxysmal atrial fibrillation and persistent atrial fibrillation ,and posses-ses certain guiding significance for outcome .

Objective To explore the risk factors of ventilator-associated pneumonia(VAP)in neonatal patients.Methods A retrospective study was conducted on the 82 neonates who had ever received mechanical ventilation for more than 48 hours,non-conditional logistic regression analysis was performed to determine independent predictors for VAP.Results By logistic regression analysis,the following risk factors independently predicted VAP:gestational age(OR = 6.209,95%CI =2.112～16.835),duration of mechanical ventilation(OR =5.842,95%CI = 1.366～14.936),times of endotracheal suctioning(OR =4.764,95%CI = 1.416～14.736),re-intubation(OR = 6.190,95%CI = 1.669～16.019),gastric tubes retention(OR =6.794,95%CI = 1.928～19.160),treatment with CNS suppressant(OR = 4.297,95%CI = 1.002～15.216).Conclusion The neonatal VAP occoured for multiple facors,the rsik factors include gestational age,duration of mechanical ventilation,endotracheal suctioning,re-intubadon,gastric tubes retention and treatment with CNS suppressant.